◆◆◆IE_0001 detail◆◆◆

Code name	IE_0001
Locus	AT4G37780
Template	IT_0001 (total cDNA)
Donor Vector	pDONR221
Forward primer	7830 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGGCGGTGAAGAAAGGGCCATGGTCGAC)
Reverse primer	7831 (GGGGACCACTTTGTACAAGAAAGCTGGGTCCTCATTATCGTATAGAGGATAATCAATGAA)
Alignment with template seq.	1st	-	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列corresponding:kigoshi一致	2nd	-
comment
sequence	>IE_0001_888bp ATGGCGGTGAAGAAAGGGCCATGGTCGACGGAGGAAGATGCCGTGCTTAAGTCTTACATCGAAAAACACGGCACCGGCAACAACTGGATTTCCCTCCCTC AGAGAATTGGGATAAAGAGGTGCGGGAAGAGTTGTCGTTTGAGATGGCTTAATTACTTGAGGCCTAACTTAAAGCATGGAGGCTTCACCGATGAAGAAGA TTACATCATTTGCAGCCTTTACATTACTATTGGAAGCAGGTGGTCTATCATTGCTTCACAATTACCGGGAAGAACAGACAACGACATCAAAAACTATTGG AACACGAGGCTGAAGAAGAAGCTATTGAGCAAGCAAGGGAAGGCATTTCATCAACAACTTAATGTCAAATTTGAGCGTGGAACAACATCATCATCATCGA GTCAGAACCAGATCCAAATCTTTCATGATGAGAACACCAAATCGAACCAAACATTATATAATCAAGTGGTGGATCCATCAATGAGAGCTTTTGCCATGGA AGAACAAAGCATGATCAAGAATCAGATATTGGAACCATTTTCTTGGGAACCAAACAAGGTTTTGTTTGATGTTGATTATGATGCAGCTGCTTCATCTTAT CATCATCATGCATCCCCATCATTGAACTCCATGAGCAGTACTAGTAGTATTGGTACTAATAATTCATCTTTACAAATGTCTCACTACACCGTCAATCACA ATGATCATGATCAACCAGATATGTTCTTTATGGACGGGTTTGAGAATTTCCAGGCCGAGCTATTTGATGAGATAGCCAACAACAACACGGTAGAAAATGG CTTTGACGGAACCGAGATCCTGATCAATAACAACTACTTGGATCACGATATTAGCTCCTTCATTGATTATCCTCTATACGATAATGAG >IE_0001_translated MAVKKGPWSTEEDAVLKSYIEKHGTGNNWISLPQRIGIKRCGKSCRLRWLNYLRPNLKHGGFTDEEDYIICSLYITIGSRWSIIASQLPGRTDNDIKNYW NTRLKKKLLSKQGKAFHQQLNVKFERGTTSSSSSQNQIQIFHDENTKSNQTLYNQVVDPSMRAFAMEEQSMIKNQILEPFSWEPNKVLFDVDYDAAASSY HHHASPSLNSMSSTSSIGTNNSSLQMSHYTVNHNDHDQPDMFFMDGFENFQAELFDEIANNNTVENGFDGTEILINNNYLDHDISSFIDYPLYDNE
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 888/915 (97.0%)（アミノ酸配列で296/305 (97.0%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G37 ATGGGAAGAGCACCGTGCTGCGACAAGATGGCGGTGAAGAAAGGGCCATGGTCGACGGAGGAAGATGCCGTGCTTAAGTCTTACATCGAAAAACACGGCA ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 ---------------------------ATGGCGGTGAAGAAAGGGCCATGGTCGACGGAGGAAGATGCCGTGCTTAAGTCTTACATCGAAAAACACGGCA 10 20 30 40 50 60 70 110 120 130 140 150 160 170 180 190 200 AT4G37 CCGGCAACAACTGGATTTCCCTCCCTCAGAGAATTGGGATAAAGAGGTGCGGGAAGAGTTGTCGTTTGAGATGGCTTAATTACTTGAGGCCTAACTTAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 CCGGCAACAACTGGATTTCCCTCCCTCAGAGAATTGGGATAAAGAGGTGCGGGAAGAGTTGTCGTTTGAGATGGCTTAATTACTTGAGGCCTAACTTAAA 80 90 100 110 120 130 140 150 160 170 210 220 230 240 250 260 270 280 290 300 AT4G37 GCATGGAGGCTTCACCGATGAAGAAGATTACATCATTTGCAGCCTTTACATTACTATTGGAAGCAGGTGGTCTATCATTGCTTCACAATTACCGGGAAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 GCATGGAGGCTTCACCGATGAAGAAGATTACATCATTTGCAGCCTTTACATTACTATTGGAAGCAGGTGGTCTATCATTGCTTCACAATTACCGGGAAGA 180 190 200 210 220 230 240 250 260 270 310 320 330 340 350 360 370 380 390 400 AT4G37 ACAGACAACGACATCAAAAACTATTGGAACACGAGGCTGAAGAAGAAGCTATTGAGCAAGCAAGGGAAGGCATTTCATCAACAACTTAATGTCAAATTTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 ACAGACAACGACATCAAAAACTATTGGAACACGAGGCTGAAGAAGAAGCTATTGAGCAAGCAAGGGAAGGCATTTCATCAACAACTTAATGTCAAATTTG 280 290 300 310 320 330 340 350 360 370 410 420 430 440 450 460 470 480 490 500 AT4G37 AGCGTGGAACAACATCATCATCATCGAGTCAGAACCAGATCCAAATCTTTCATGATGAGAACACCAAATCGAACCAAACATTATATAATCAAGTGGTGGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 AGCGTGGAACAACATCATCATCATCGAGTCAGAACCAGATCCAAATCTTTCATGATGAGAACACCAAATCGAACCAAACATTATATAATCAAGTGGTGGA 380 390 400 410 420 430 440 450 460 470 510 520 530 540 550 560 570 580 590 600 AT4G37 TCCATCAATGAGAGCTTTTGCCATGGAAGAACAAAGCATGATCAAGAATCAGATATTGGAACCATTTTCTTGGGAACCAAACAAGGTTTTGTTTGATGTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 TCCATCAATGAGAGCTTTTGCCATGGAAGAACAAAGCATGATCAAGAATCAGATATTGGAACCATTTTCTTGGGAACCAAACAAGGTTTTGTTTGATGTT 480 490 500 510 520 530 540 550 560 570 610 620 630 640 650 660 670 680 690 700 AT4G37 GATTATGATGCAGCTGCTTCATCTTATCATCATCATGCATCCCCATCATTGAACTCCATGAGCAGTACTAGTAGTATTGGTACTAATAATTCATCTTTAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 GATTATGATGCAGCTGCTTCATCTTATCATCATCATGCATCCCCATCATTGAACTCCATGAGCAGTACTAGTAGTATTGGTACTAATAATTCATCTTTAC 580 590 600 610 620 630 640 650 660 670 710 720 730 740 750 760 770 780 790 800 AT4G37 AAATGTCTCACTACACCGTCAATCACAATGATCATGATCAACCAGATATGTTCTTTATGGACGGGTTTGAGAATTTCCAGGCCGAGCTATTTGATGAGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 AAATGTCTCACTACACCGTCAATCACAATGATCATGATCAACCAGATATGTTCTTTATGGACGGGTTTGAGAATTTCCAGGCCGAGCTATTTGATGAGAT 680 690 700 710 720 730 740 750 760 770 810 820 830 840 850 860 870 880 890 900 AT4G37 AGCCAACAACAACACGGTAGAAAATGGCTTTGACGGAACCGAGATCCTGATCAATAACAACTACTTGGATCACGATATTAGCTCCTTCATTGATTATCCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 AGCCAACAACAACACGGTAGAAAATGGCTTTGACGGAACCGAGATCCTGATCAATAACAACTACTTGGATCACGATATTAGCTCCTTCATTGATTATCCT 780 790 800 810 820 830 840 850 860 870 910 AT4G37 CTATACGATAATGAG ::::::::::::::: IE_000 CTATACGATAATGAG 880 10 20 30 40 50 60 70 80 90 100 AT4G37 MGRAPCCDKMAVKKGPWSTEEDAVLKSYIEKHGTGNNWISLPQRIGIKRCGKSCRLRWLNYLRPNLKHGGFTDEEDYIICSLYITIGSRWSIIASQLPGR ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 ---------MAVKKGPWSTEEDAVLKSYIEKHGTGNNWISLPQRIGIKRCGKSCRLRWLNYLRPNLKHGGFTDEEDYIICSLYITIGSRWSIIASQLPGR 10 20 30 40 50 60 70 80 90 110 120 130 140 150 160 170 180 190 200 AT4G37 TDNDIKNYWNTRLKKKLLSKQGKAFHQQLNVKFERGTTSSSSSQNQIQIFHDENTKSNQTLYNQVVDPSMRAFAMEEQSMIKNQILEPFSWEPNKVLFDV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 TDNDIKNYWNTRLKKKLLSKQGKAFHQQLNVKFERGTTSSSSSQNQIQIFHDENTKSNQTLYNQVVDPSMRAFAMEEQSMIKNQILEPFSWEPNKVLFDV 100 110 120 130 140 150 160 170 180 190 210 220 230 240 250 260 270 280 290 300 AT4G37 DYDAAASSYHHHASPSLNSMSSTSSIGTNNSSLQMSHYTVNHNDHDQPDMFFMDGFENFQAELFDEIANNNTVENGFDGTEILINNNYLDHDISSFIDYP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 DYDAAASSYHHHASPSLNSMSSTSSIGTNNSSLQMSHYTVNHNDHDQPDMFFMDGFENFQAELFDEIANNNTVENGFDGTEILINNNYLDHDISSFIDYP 200 210 220 230 240 250 260 270 280 290 AT4G37 LYDNE ::::: IE_000 LYDNE

このページのTOPに戻る