◆◆◆IE_0008 detail◆◆◆

Code name	IE_0008
Locus	AT3G01140
Template	IT_0008 (HR2112)
Donor Vector	pDONR207
Forward primer	6644 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGGGCAGATCGCCATGTTG)
Reverse primer	6655 (GGGGACCACTTTGTACAAGAAAGCTGGGTCGAACATCGTCGCGGAATCGGACGGTGAA)
Alignment with template seq.	1st	-	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列corresponding:ooshima一致	2nd	-
comment
sequence	>IE_0008_1035bp ATGGGCAGATCGCCATGTTGTGATAAGGCCGGGTTGAAGAAAGGGCCTTGGACTCCAGAAGAGGATCAGAAACTTTTGGCTTATATTGAAGAACATGGCC ATGGAAGCTGGCGTTCTTTGCCTGAGAAAGCCGGTCTCCAAAGGTGTGGAAAGAGTTGCAGACTCAGATGGACTAACTACCTAAGACCTGACATCAAGAG AGGCAAATTCACTGTACAAGAAGAACAAACCATCATTCAACTCCACGCTCTCCTCGGAAACAGGTGGTCAGCGATTGCAACTCATTTACCAAAGAGGACA GACAACGAGATCAAGAACTACTGGAACACACACTTGAAGAAACGTCTGATCAAAATGGGGATAGATCCAGTGACTCACAAGCACAAAAACGAGACTCTTT CGTCTTCCACAGGACAATCAAAGAACGCAGCCACGCTTAGTCATATGGCTCAATGGGAGAGTGCAAGACTCGAAGCTGAAGCAAGGCTAGCTAGAGAATC AAAGCTTCTCCATTTACAGCATTACCAAAACAATAACAACCTTAACAAATCAGCAGCTCCTCAACAACATTGCTTCACTCAAAAAACATCAACAAACTGG ACTAAACCAAACCAAGGAAACGGAGACCAACAGCTTGAATCTCCGACATCGACGGTGACATTCTCTGAGAATCTTCTGATGCCTTTAGGAATCCCTACGG ATAGCAGCAGAAATAGAAACAATAACAACAATGAGTCCTCGGCGATGATTGAATTGGCCGTATCTTCGTCAACCTCCTCCGATGTGAGTCTGGTCAAAGA ACATGAACACGACTGGATTAGGCAGATCAACTGTGGTAGTGGAGGAATAGGAGAAGGATTCACGAGTCTATTGATCGGTGATTCGGTCGGCCGGGGTTTA CCCACCGGGAAAAACGAAGCGACGGCGGGCGTGGGGAATGAGAGTGAGTATAACTACTATGAGGATAACAAGAATTACTGGAATAGCATTCTCAACTTGG TTGATTCTTCACCGTCCGATTCCGCGACGATGTTC >IE_0008_translated MGRSPCCDKAGLKKGPWTPEEDQKLLAYIEEHGHGSWRSLPEKAGLQRCGKSCRLRWTNYLRPDIKRGKFTVQEEQTIIQLHALLGNRWSAIATHLPKRT DNEIKNYWNTHLKKRLIKMGIDPVTHKHKNETLSSSTGQSKNAATLSHMAQWESARLEAEARLARESKLLHLQHYQNNNNLNKSAAPQQHCFTQKTSTNW TKPNQGNGDQQLESPTSTVTFSENLLMPLGIPTDSSRNRNNNNNESSAMIELAVSSSTSSDVSLVKEHEHDWIRQINCGSGGIGEGFTSLLIGDSVGRGL PTGKNEATAGVGNESEYNYYEDNKNYWNSILNLVDSSPSDSATMF
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1032/1164 (88.7%)（アミノ酸配列で343/388 (88.4%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT3G01 ATGCCAATCCATGTCAGAGACCGGGAAAAGGGGCGCTTACAGAACCTAAACAGAGACATCTTCTGTTGTGTTTCTCCATCTATATATCAGAGTGACGCCA IE_000 ---------------------------------------------------------------------------------------------------- 110 120 130 140 150 160 170 180 190 200 AT3G01 AGAGAGCAGCTTTTGTGATCATACTGATCATGATCATATCGCCATGTTGTGATAAGGCCGGGTTGAAGAAAGGGCCTTGGACTCCAGAAGAGGATCAGAA ::: :: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 -----------------------------ATGGGCAGATCGCCATGTTGTGATAAGGCCGGGTTGAAGAAAGGGCCTTGGACTCCAGAAGAGGATCAGAA 10 20 30 40 50 60 70 210 220 230 240 250 260 270 280 290 300 AT3G01 ACTTTTGGCTTATATTGAAGAACATGGCCATGGAAGCTGGCGTTCTTTGCCTGAGAAAGCCGGTCTCCAAAGGTGTGGAAAGAGTTGCAGACTCAGATGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 ACTTTTGGCTTATATTGAAGAACATGGCCATGGAAGCTGGCGTTCTTTGCCTGAGAAAGCCGGTCTCCAAAGGTGTGGAAAGAGTTGCAGACTCAGATGG 80 90 100 110 120 130 140 150 160 170 310 320 330 340 350 360 370 380 390 400 AT3G01 ACTAACTACCTAAGACCTGACATCAAGAGAGGCAAATTCACTGTACAAGAAGAACAAACCATCATTCAACTCCACGCTCTCCTCGGAAACAGGTGGTCAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 ACTAACTACCTAAGACCTGACATCAAGAGAGGCAAATTCACTGTACAAGAAGAACAAACCATCATTCAACTCCACGCTCTCCTCGGAAACAGGTGGTCAG 180 190 200 210 220 230 240 250 260 270 410 420 430 440 450 460 470 480 490 500 AT3G01 CGATTGCAACTCATTTACCAAAGAGGACAGACAACGAGATCAAGAACTACTGGAACACACACTTGAAGAAACGTCTGATCAAAATGGGGATAGATCCAGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 CGATTGCAACTCATTTACCAAAGAGGACAGACAACGAGATCAAGAACTACTGGAACACACACTTGAAGAAACGTCTGATCAAAATGGGGATAGATCCAGT 280 290 300 310 320 330 340 350 360 370 510 520 530 540 550 560 570 580 590 600 AT3G01 GACTCACAAGCACAAAAACGAGACTCTTTCGTCTTCCACAGGACAATCAAAGAACGCAGCCACGCTTAGTCATATGGCTCAATGGGAGAGTGCAAGACTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 GACTCACAAGCACAAAAACGAGACTCTTTCGTCTTCCACAGGACAATCAAAGAACGCAGCCACGCTTAGTCATATGGCTCAATGGGAGAGTGCAAGACTC 380 390 400 410 420 430 440 450 460 470 610 620 630 640 650 660 670 680 690 700 AT3G01 GAAGCTGAAGCAAGGCTAGCTAGAGAATCAAAGCTTCTCCATTTACAGCATTACCAAAACAATAACAACCTTAACAAATCAGCAGCTCCTCAACAACATT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 GAAGCTGAAGCAAGGCTAGCTAGAGAATCAAAGCTTCTCCATTTACAGCATTACCAAAACAATAACAACCTTAACAAATCAGCAGCTCCTCAACAACATT 480 490 500 510 520 530 540 550 560 570 710 720 730 740 750 760 770 780 790 800 AT3G01 GCTTCACTCAAAAAACATCAACAAACTGGACTAAACCAAACCAAGGAAACGGAGACCAACAGCTTGAATCTCCGACATCGACGGTGACATTCTCTGAGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 GCTTCACTCAAAAAACATCAACAAACTGGACTAAACCAAACCAAGGAAACGGAGACCAACAGCTTGAATCTCCGACATCGACGGTGACATTCTCTGAGAA 580 590 600 610 620 630 640 650 660 670 810 820 830 840 850 860 870 880 890 900 AT3G01 TCTTCTGATGCCTTTAGGAATCCCTACGGATAGCAGCAGAAATAGAAACAATAACAACAATGAGTCCTCGGCGATGATTGAATTGGCCGTATCTTCGTCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 TCTTCTGATGCCTTTAGGAATCCCTACGGATAGCAGCAGAAATAGAAACAATAACAACAATGAGTCCTCGGCGATGATTGAATTGGCCGTATCTTCGTCA 680 690 700 710 720 730 740 750 760 770 910 920 930 940 950 960 970 980 990 1000 AT3G01 ACCTCCTCCGATGTGAGTCTGGTCAAAGAACATGAACACGACTGGATTAGGCAGATCAACTGTGGTAGTGGAGGAATAGGAGAAGGATTCACGAGTCTAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 ACCTCCTCCGATGTGAGTCTGGTCAAAGAACATGAACACGACTGGATTAGGCAGATCAACTGTGGTAGTGGAGGAATAGGAGAAGGATTCACGAGTCTAT 780 790 800 810 820 830 840 850 860 870 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT3G01 TGATCGGTGATTCGGTCGGCCGGGGTTTACCCACCGGGAAAAACGAAGCGACGGCGGGCGTGGGGAATGAGAGTGAGTATAACTACTATGAGGATAACAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 TGATCGGTGATTCGGTCGGCCGGGGTTTACCCACCGGGAAAAACGAAGCGACGGCGGGCGTGGGGAATGAGAGTGAGTATAACTACTATGAGGATAACAA 880 890 900 910 920 930 940 950 960 970 1110 1120 1130 1140 1150 1160 AT3G01 GAATTACTGGAATAGCATTCTCAACTTGGTTGATTCTTCACCGTCCGATTCCGCGACGATGTTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 GAATTACTGGAATAGCATTCTCAACTTGGTTGATTCTTCACCGTCCGATTCCGCGACGATGTTC 980 990 1000 1010 1020 1030 10 20 30 40 50 60 70 80 90 100 AT3G01 MPIHVRDREKGRLQNLNRDIFCCVSPSIYQSDAKRAAFVIILIMIISPCCDKAGLKKGPWTPEEDQKLLAYIEEHGHGSWRSLPEKAGLQRCGKSCRLRW : :::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 -------------------------------------------MGRSPCCDKAGLKKGPWTPEEDQKLLAYIEEHGHGSWRSLPEKAGLQRCGKSCRLRW 10 20 30 40 50 110 120 130 140 150 160 170 180 190 200 AT3G01 TNYLRPDIKRGKFTVQEEQTIIQLHALLGNRWSAIATHLPKRTDNEIKNYWNTHLKKRLIKMGIDPVTHKHKNETLSSSTGQSKNAATLSHMAQWESARL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 TNYLRPDIKRGKFTVQEEQTIIQLHALLGNRWSAIATHLPKRTDNEIKNYWNTHLKKRLIKMGIDPVTHKHKNETLSSSTGQSKNAATLSHMAQWESARL 60 70 80 90 100 110 120 130 140 150 210 220 230 240 250 260 270 280 290 300 AT3G01 EAEARLARESKLLHLQHYQNNNNLNKSAAPQQHCFTQKTSTNWTKPNQGNGDQQLESPTSTVTFSENLLMPLGIPTDSSRNRNNNNNESSAMIELAVSSS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 EAEARLARESKLLHLQHYQNNNNLNKSAAPQQHCFTQKTSTNWTKPNQGNGDQQLESPTSTVTFSENLLMPLGIPTDSSRNRNNNNNESSAMIELAVSSS 160 170 180 190 200 210 220 230 240 250 310 320 330 340 350 360 370 380 AT3G01 TSSDVSLVKEHEHDWIRQINCGSGGIGEGFTSLLIGDSVGRGLPTGKNEATAGVGNESEYNYYEDNKNYWNSILNLVDSSPSDSATMF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_000 TSSDVSLVKEHEHDWIRQINCGSGGIGEGFTSLLIGDSVGRGLPTGKNEATAGVGNESEYNYYEDNKNYWNSILNLVDSSPSDSATMF 260 270 280 290 300 310 320 330 340

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