◆◆◆IE_0022 detail◆◆◆

Code nameIE_0022
LocusAT2G46510
TemplateIT_0022 (cDNA)
Donor VectorpDONR207
Forward primer5821 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGAATATGAGTGATTTAGGTTGGGATG)
Reverse primer5822 (GGGGACCACTTTGTACAAGAAAGCTGGGTCTATATCACCAGAGACCTGTGAACTAGAAG)
Alignment with template seq.1st-2nd-
Alignment with TAIR7CDSアミノ酸配列corresponding:nakata一致-
commentbHLH017new, JAM1, 既存のはエラーがあったため
sequence 
>IE_0022_1698bp
ATGAATATGAGTGATTTAGGTTGGGATGATGAAGATAAATCGGTGGTTAGTGCTGTTTTAGGGCATTTAGCTTCTGATTTTCTTCGAGCAAACTCTAATT
CGAATCAGAATCTCTTTCTTGTTATGGGAACTGATGATACTCTGAATAAGAAGCTCTCTAGTCTCGTTGATTGGCCAAACTCGGAGAATTTCAGCTGGAA
CTACGCTATTTTCTGGCAACAAACCATGTCTAGATCCGGACAACAAGTCTTAGGTTGGGGAGATGGGTGTTGTCGAGAGCCTAATGAGGAAGAGGAATCA
AAAGTTGTTAGGTCTTATAATTTTAACAACATGGGGGCAGAGGAAGAGACATGGCAAGATATGAGGAAGAGAGTGTTGCAGAAGCTTCATAGGTTGTTTG
GTGGATCTGATGAAGACAATTATGCTTTGAGCTTAGAGAAAGTTACTGCTACTGAGATTTTCTTCTTAGCTTCCATGTATTTCTTCTTCAATCACGGTGA
AGGCGGTCCTGGGAGGTGTTATTCTTCAGGGAAACATGTGTGGCTCTCTGATGCAGTTAACTCTGAGTCTGACTATTGTTTCAGGTCTTTTATGGCGAAA
TCTGCGGGAATCAGAACGATCGTTATGGTTCCTACTGATGCTGGTGTTCTTGAGCTTGGTTCTGTTTGGTCTTTGCCTGAAAACATTGGCTTGGTTAAGT
CTGTTCAAGCTTTGTTCATGAGGAGAGTTACGCAACCAGTAATGGTGACTTCAAACACTAACATGACTGGAGGGATTCACAAGCTTTTCGGGCAGGATTT
GAGTGGAGCTCACGCGTATCCTAAGAAGCTCGAAGTGAGAAGAAACTTGGATGAGAGATTCACTCCTCAAAGTTGGGAAGGCTATAATAACAATAAAGGT
CCAACATTTGGTTACACACCTCAGAGGGATGATGTGAAAGTGCTAGAGAATGTGAATATGGTTGTAGATAATAACAATTACAAGACGCAGATTGAGTTTG
CGGGATCATCAGTTGCTGCTTCTTCGAATCCATCTACAAACACTCAGCAAGAAAAATCAGAATCTTGTACAGAGAAAAGACCAGTGAGCTTGTTAGCAGG
AGCAGGAATAGTTTCTGTTGTTGATGAGAAGAGACCGAGAAAGAGAGGGAGAAAGCCTGCAAACGGAAGAGAAGAGCCATTGAACCATGTGGAAGCTGAG
AGGCAGAGACGCGAGAAGCTTAACCAAAGATTCTACGCTTTACGATCAGTTGTTCCAAACATTTCTAAAATGGACAAGGCTTCTCTACTTGGAGACGCAA
TTTCTTACATCAAAGAGCTTCAAGAGAAAGTCAAGATAATGGAAGATGAAAGAGTAGGAACAGATAAGAGCTTATCAGAATCAAACACAATAACAGTAGA
AGAAAGTCCAGAAGTTGACATTCAAGCTATGAATGAAGAGGTTGTTGTAAGAGTAATCTCGCCTTTGGATTCACATCCAGCTTCAAGAATCATACAAGCA
ATGAGAAACTCAAATGTTAGTCTAATGGAGGCTAAGTTATCATTAGCTGAAGACACAATGTTTCACACTTTTGTGATAAAGTCTAACAACGGGTCGGATC
CATTGACGAAAGAGAAGCTTATAGCAGCGTTTTACCCCGAGACCAGCTCGACGCAACCGCCATTGCCTTCTTCTAGTTCACAGGTCTCTGGTGATATA

>IE_0022_translated
MNMSDLGWDDEDKSVVSAVLGHLASDFLRANSNSNQNLFLVMGTDDTLNKKLSSLVDWPNSENFSWNYAIFWQQTMSRSGQQVLGWGDGCCREPNEEEES
KVVRSYNFNNMGAEEETWQDMRKRVLQKLHRLFGGSDEDNYALSLEKVTATEIFFLASMYFFFNHGEGGPGRCYSSGKHVWLSDAVNSESDYCFRSFMAK
SAGIRTIVMVPTDAGVLELGSVWSLPENIGLVKSVQALFMRRVTQPVMVTSNTNMTGGIHKLFGQDLSGAHAYPKKLEVRRNLDERFTPQSWEGYNNNKG
PTFGYTPQRDDVKVLENVNMVVDNNNYKTQIEFAGSSVAASSNPSTNTQQEKSESCTEKRPVSLLAGAGIVSVVDEKRPRKRGRKPANGREEPLNHVEAE
RQRREKLNQRFYALRSVVPNISKMDKASLLGDAISYIKELQEKVKIMEDERVGTDKSLSESNTITVEESPEVDIQAMNEEVVVRVISPLDSHPASRIIQA
MRNSNVSLMEAKLSLAEDTMFHTFVIKSNNGSDPLTKEKLIAAFYPETSSTQPPLPSSSSQVSGDI
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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