◆◆◆IE_0024 detail◆◆◆

Code nameIE_0024
LocusAT2G22770
TemplateIT_0024 (cDNA)
Donor VectorpDONR207
Forward primer7342 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGGATGATTCAAGCTTTATGGA)
Reverse primerOperon6C1 (ggggaccactttgtacaagaaagctgggttTTCAGCTAACGCAACTCTTATGTTCTTCAC)
Alignment with template seq.1st-2nd-
Alignment with TAIR7CDSアミノ酸配列corresponding:nakata一致-
commentNAI1new, アノテーションが変わって、N末側が長くなったため
sequence 
>IE_0024_960bp
ATGGATGATTCAAGCTTTATGGATTTGATGATCGACACTGACGAGTATCTGATCGATGACTGGGAATCCGATTTCCCGATATGCGGGGAAACTAACACCA
ACCCCGGTTCCGAGTCTGGATCTGGAACCGGGTTTGAGTTGCTAGCAGAAAGACCAACGAAGCAAATGAAAACCAACAACAACATGAATTCAACATCTTC
TTCTCCGTCGTCTTCTTCTTCTTCTGGTTCTCGCACGTCACAAGTGATCTCATTTGGGTCTCCAGACACGAAAACCAACCCGGTCGAGACATCTTTGAAC
TTCTCCAACCAAGTAAGCATGGATCAGAAAGTGGGGTCGAAAAGAAAAGATTGTGTTAACAATGGAGGAAGAAGAGAACCACATCTATTGAAAGAACATG
TTTTGGCTGAACGAAAACGTCGACAAAAGCTCAACGAGCGTTTGATTGCTCTCTCTGCTCTTCTTCCTGGCCTCAAAAAGACGGACAAGGCAACAGTTCT
TGAAGATGCCATCAAACATTTGAAACAACTTCAAGAGCGGGTAAAGAAGCTGGAGGAGGAGCGAGTGGTGACCAAGAAAATGGATCAATCAATTATATTG
GTGAAGAGATCTCAAGTGTATTTAGATGATGATTCTTCATCTTATTCTTCTACTTGCTCTGCTGCGTCTCCTCTGTCTTCTTCTTCGGACGAAGTTTCGA
TCTTCAAACAAACAATGCCTATGATCGAAGCCCGAGTTTCAGACAGAGATTTGTTGATTAGAGTCCATTGTGAGAAGAACAAAGGGTGTATGATTAAGAT
CTTAAGTTCCTTGGAGAAGTTTCGTCTTGAAGTAGTCAATAGCTTCACTTTACCATTTGGAAATTCAACTCTCGTTATAACCATTCTCACTAAGATGGAC
AACAAATTTTCTCGACCAGTTGAAGAAGTGGTGAAGAACATAAGAGTTGCGTTAGCTGAA

>IE_0024_translated
MDDSSFMDLMIDTDEYLIDDWESDFPICGETNTNPGSESGSGTGFELLAERPTKQMKTNNNMNSTSSSPSSSSSSGSRTSQVISFGSPDTKTNPVETSLN
FSNQVSMDQKVGSKRKDCVNNGGRREPHLLKEHVLAERKRRQKLNERLIALSALLPGLKKTDKATVLEDAIKHLKQLQERVKKLEEERVVTKKMDQSIIL
VKRSQVYLDDDSSSYSSTCSAASPLSSSSDEVSIFKQTMPMIEARVSDRDLLIRVHCEKNKGCMIKILSSLEKFRLEVVNSFTLPFGNSTLVITILTKMD
NKFSRPVEEVVKNIRVALAE
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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