◆◆◆IE_0025 detail◆◆◆

Code name	IE_0025
Locus	AT3G17860
Template	IT_0025 (cDNA)
Donor Vector	pDONR207
Forward primer	5259 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGGAGAGAGATTTTCTCGG)
Reverse primer	8130 (GGGGACCACTTTGTACAAGAAAGCTGGGTCAAGAAGTGTTGCGTACCTTTCTTTGCGT)
Alignment with template seq.	1st	-	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列corresponding:nakata一致	2nd	-
comment	JAZ3.4
sequence	>IE_0025_957bp ATGAACTGGTCTTTCTCAAACAAAGTATCAGCTTCTTCTTCTCAGTTTCTATCCTTCAGGCCAACTCAAGAAGATAGACATAGAAAGTCTGGAAATTATC ATCTTCCTCACTCTGGTTCCTTCATGCCATCATCAGTAGCTGATGTTTATGATTCAACCCGCAAAGCTCCTTACAGTTCTGTACAGGGAGTGAGGATGTT CCCTAATTCCAATCAACACGAAGAAACTAACGCAGTTTCCATGTCGATGCCGGGTTTCCAGTCTCATCATTATGCACCAGGAGGAAGAAGCTTCATGAAC AATAACAATAACTCACAACCTTTGGTAGGAGTTCCTATCATGGCACCTCCAATTTCAATCCTTCCTCCTCCAGGTTCCATTGTAGGGACTACTGATATTA GATCTTCTTCCAAGCCAATAGGTTCACCTGCGCAGTTGACGATCTTTTATGCCGGTTCAGTTTGTGTTTACGATGACATATCTCCTGAAAAGGCAAAGGC GATAATGTTGCTAGCTGGGAACGGTTCCTCTATGCCTCAAGTCTTTTCGCCGCCTCAAACTCATCAACAAGTGGTCCATCATACTCGTGCCTCTGTCGAT TCTTCAGCTATGCCTCCTAGCTTCATGCCTACAATATCTTATCTTAGCCCTGAAGCTGGAAGTAGCACAAACGGACTCGGAGCCACAAAAGCGACAAGAG GCTTGACGTCAACATATCACAACAACCAAGCTAATGGATCCAATATTAACTGCCCAGTACCAGTTTCTTGTTCTACCAATGTAATGGCTCCAACAGTGGC ATTACCTCTGGCTCGCAAAGCATCCCTGGCTAGGTTTTTAGAGAAACGCAAAGAAAGGGTCACGAGCGTATCCCCATATTGCTTAGACAAGAAGTCATCG ACAGATTGTCGCAGATCAATGTCTGAATGCATTAGTTCTTCTCTCAGCTCTGCAACC >IE_0025_translated MNWSFSNKVSASSSQFLSFRPTQEDRHRKSGNYHLPHSGSFMPSSVADVYDSTRKAPYSSVQGVRMFPNSNQHEETNAVSMSMPGFQSHHYAPGGRSFMN NNNNSQPLVGVPIMAPPISILPPPGSIVGTTDIRSSSKPIGSPAQLTIFYAGSVCVYDDISPEKAKAIMLLAGNGSSMPQVFSPPQTHQQVVHHTRASVD SSAMPPSFMPTISYLSPEAGSSTNGLGATKATRGLTSTYHNNQANGSNINCPVPVSCSTNVMAPTVALPLARKASLARFLEKRKERVTSVSPYCLDKKSS TDCRRSMSECISSSLSSAT
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 957/1056 (90.6%)（アミノ酸配列で319/352 (90.6%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT3G17 ATGGAGAGAGATTTTCTCGGGTTGGGTTCGAAAAATTCTCCGATCACTGTCAAGGAGGAAACCAGCGAAAGCTCTAGAGATTCAGCTCCCAACAGAGGAA : IE_002 ---------------------------------------------------------------------------------------------------A 110 120 130 140 150 160 170 180 190 200 AT3G17 TGAACTGGTCTTTCTCAAACAAAGTATCAGCTTCTTCTTCTCAGTTTCTATCCTTCAGGCCAACTCAAGAAGATAGACATAGAAAGTCTGGAAATTATCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 TGAACTGGTCTTTCTCAAACAAAGTATCAGCTTCTTCTTCTCAGTTTCTATCCTTCAGGCCAACTCAAGAAGATAGACATAGAAAGTCTGGAAATTATCA 10 20 30 40 50 60 70 80 90 100 210 220 230 240 250 260 270 280 290 300 AT3G17 TCTTCCTCACTCTGGTTCCTTCATGCCATCATCAGTAGCTGATGTTTATGATTCAACCCGCAAAGCTCCTTACAGTTCTGTACAGGGAGTGAGGATGTTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 TCTTCCTCACTCTGGTTCCTTCATGCCATCATCAGTAGCTGATGTTTATGATTCAACCCGCAAAGCTCCTTACAGTTCTGTACAGGGAGTGAGGATGTTC 110 120 130 140 150 160 170 180 190 200 310 320 330 340 350 360 370 380 390 400 AT3G17 CCTAATTCCAATCAACACGAAGAAACTAACGCAGTTTCCATGTCGATGCCGGGTTTCCAGTCTCATCATTATGCACCAGGAGGAAGAAGCTTCATGAACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 CCTAATTCCAATCAACACGAAGAAACTAACGCAGTTTCCATGTCGATGCCGGGTTTCCAGTCTCATCATTATGCACCAGGAGGAAGAAGCTTCATGAACA 210 220 230 240 250 260 270 280 290 300 410 420 430 440 450 460 470 480 490 500 AT3G17 ATAACAATAACTCACAACCTTTGGTAGGAGTTCCTATCATGGCACCTCCAATTTCAATCCTTCCTCCTCCAGGTTCCATTGTAGGGACTACTGATATTAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 ATAACAATAACTCACAACCTTTGGTAGGAGTTCCTATCATGGCACCTCCAATTTCAATCCTTCCTCCTCCAGGTTCCATTGTAGGGACTACTGATATTAG 310 320 330 340 350 360 370 380 390 400 510 520 530 540 550 560 570 580 590 600 AT3G17 ATCTTCTTCCAAGCCAATAGGTTCACCTGCGCAGTTGACGATCTTTTATGCCGGTTCAGTTTGTGTTTACGATGACATATCTCCTGAAAAGGCAAAGGCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 ATCTTCTTCCAAGCCAATAGGTTCACCTGCGCAGTTGACGATCTTTTATGCCGGTTCAGTTTGTGTTTACGATGACATATCTCCTGAAAAGGCAAAGGCG 410 420 430 440 450 460 470 480 490 500 610 620 630 640 650 660 670 680 690 700 AT3G17 ATAATGTTGCTAGCTGGGAACGGTTCCTCTATGCCTCAAGTCTTTTCGCCGCCTCAAACTCATCAACAAGTGGTCCATCATACTCGTGCCTCTGTCGATT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 ATAATGTTGCTAGCTGGGAACGGTTCCTCTATGCCTCAAGTCTTTTCGCCGCCTCAAACTCATCAACAAGTGGTCCATCATACTCGTGCCTCTGTCGATT 510 520 530 540 550 560 570 580 590 600 710 720 730 740 750 760 770 780 790 800 AT3G17 CTTCAGCTATGCCTCCTAGCTTCATGCCTACAATATCTTATCTTAGCCCTGAAGCTGGAAGTAGCACAAACGGACTCGGAGCCACAAAAGCGACAAGAGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 CTTCAGCTATGCCTCCTAGCTTCATGCCTACAATATCTTATCTTAGCCCTGAAGCTGGAAGTAGCACAAACGGACTCGGAGCCACAAAAGCGACAAGAGG 610 620 630 640 650 660 670 680 690 700 810 820 830 840 850 860 870 880 890 900 AT3G17 CTTGACGTCAACATATCACAACAACCAAGCTAATGGATCCAATATTAACTGCCCAGTACCAGTTTCTTGTTCTACCAATGTAATGGCTCCAACAGTGGCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 CTTGACGTCAACATATCACAACAACCAAGCTAATGGATCCAATATTAACTGCCCAGTACCAGTTTCTTGTTCTACCAATGTAATGGCTCCAACAGTGGCA 710 720 730 740 750 760 770 780 790 800 910 920 930 940 950 960 970 980 990 1000 AT3G17 TTACCTCTGGCTCGCAAAGCATCCCTGGCTAGGTTTTTAGAGAAACGCAAAGAAAGGGTCACGAGCGTATCCCCATATTGCTTAGACAAGAAGTCATCGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 TTACCTCTGGCTCGCAAAGCATCCCTGGCTAGGTTTTTAGAGAAACGCAAAGAAAGGGTCACGAGCGTATCCCCATATTGCTTAGACAAGAAGTCATCGA 810 820 830 840 850 860 870 880 890 900 1010 1020 1030 1040 1050 AT3G17 CAGATTGTCGCAGATCAATGTCTGAATGCATTAGTTCTTCTCTCAGCTCTGCAACC :::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 CAGATTGTCGCAGATCAATGTCTGAATGCATTAGTTCTTCTCTCAGCTCTGCAACC 910 920 930 940 950 10 20 30 40 50 60 70 80 90 100 AT3G17 MERDFLGLGSKNSPITVKEETSESSRDSAPNRGMNWSFSNKVSASSSQFLSFRPTQEDRHRKSGNYHLPHSGSFMPSSVADVYDSTRKAPYSSVQGVRMF ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 ---------------------------------MNWSFSNKVSASSSQFLSFRPTQEDRHRKSGNYHLPHSGSFMPSSVADVYDSTRKAPYSSVQGVRMF 10 20 30 40 50 60 110 120 130 140 150 160 170 180 190 200 AT3G17 PNSNQHEETNAVSMSMPGFQSHHYAPGGRSFMNNNNNSQPLVGVPIMAPPISILPPPGSIVGTTDIRSSSKPIGSPAQLTIFYAGSVCVYDDISPEKAKA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 PNSNQHEETNAVSMSMPGFQSHHYAPGGRSFMNNNNNSQPLVGVPIMAPPISILPPPGSIVGTTDIRSSSKPIGSPAQLTIFYAGSVCVYDDISPEKAKA 70 80 90 100 110 120 130 140 150 160 210 220 230 240 250 260 270 280 290 300 AT3G17 IMLLAGNGSSMPQVFSPPQTHQQVVHHTRASVDSSAMPPSFMPTISYLSPEAGSSTNGLGATKATRGLTSTYHNNQANGSNINCPVPVSCSTNVMAPTVA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 IMLLAGNGSSMPQVFSPPQTHQQVVHHTRASVDSSAMPPSFMPTISYLSPEAGSSTNGLGATKATRGLTSTYHNNQANGSNINCPVPVSCSTNVMAPTVA 170 180 190 200 210 220 230 240 250 260 310 320 330 340 350 AT3G17 LPLARKASLARFLEKRKERVTSVSPYCLDKKSSTDCRRSMSECISSSLSSAT :::::::::::::::::::::::::::::::::::::::::::::::::::: IE_002 LPLARKASLARFLEKRKERVTSVSPYCLDKKSSTDCRRSMSECISSSLSSAT 270 280 290 300 310

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