| Code name | IE_0027 | |||
|---|---|---|---|---|
| Locus | AT5G13220 | |||
| Template | IT_0027 (cDNA) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | 5267 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGTCGAAAGCTACCATAGAAC) | |||
| Reverse primer | 8128 (GGGGACCACTTTGTACAAGAAAGCTGGGTCATCTCTCCTTGCGCTTCTCGAGAAAACG) | |||
| Alignment with template seq. | 1st | - | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列corresponding:nakata一致 | - | ||
| comment | JAZ10.4 | |||
| sequence |
>IE_0027_501bp ATGTCGAAAGCTACCATAGAACTCGATTTCCTCGGACTTGAGAAGAAACAAACCAACAACGCTCCTAAGCCTAAGTTCCAGAAATTTCTCGATCGCCGTC GTAGTTTCCGAGATATTCAAGGTGCGATTTCGAAAATCGATCCGGAGATTATCAAATCGCTGTTAGCTTCCACTGGAAACAATTCCGATTCATCGGCTAA ATCTCGTTCGGTTCCGTCTACTCCGAGGGAAGATCAGCCTCAGATCCCGATTTCTCCGGTCCACGCGTCTCTCGCCAGGTCTAGTACCGAACTCGTTTCG GGAACTGTTCCTATGACGATTTTCTACAATGGAAGTGTTTCAGTTTTCCAAGTGTCTCGTAACAAAGCTGGTGAAATTATGAAGGTCGCTAATGAAGCAG CATCTAAGAAAGACGAGTCGTCGATGGAGACAGATCTTTCGATCTTCCCATCGCAAGGAGAAAGTCACTGCAACGTTTTCTCGAGAAGCGCAAGGAGAGA T >IE_0027_translated MSKATIELDFLGLEKKQTNNAPKPKFQKFLDRRRSFRDIQGAISKIDPEIIKSLLASTGNNSDSSAKSRSVPSTPREDQPQIPISPVHASLARSSTELVS GTVPMTIFYNGSVSVFQVSRNKAGEIMKVANEAASKKDESSMETDLSIFPSQGESHCNVFSRSARRD |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 501/591 (84.8%)（アミノ酸配列で151/199 (75.9%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT5G13 ATGTCGAAAGCTACCATAGAACTCGATTTCCTCGGACTTGAGAAGAAACAAACCAACAACGCTCCTAAGCCTAAGTTCCAGAAATTTCTCGATCGCCGTC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_002 ATGTCGAAAGCTACCATAGAACTCGATTTCCTCGGACTTGAGAAGAAACAAACCAACAACGCTCCTAAGCCTAAGTTCCAGAAATTTCTCGATCGCCGTC
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT5G13 GTAGTTTCCGAGATATTCAAGGTGCGATTTCGAAAATCGATCCGGAGATTATCAAATCGCTGTTAGCTTCCACTGGAAACAATTCCGATTCATCGGCTAA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_002 GTAGTTTCCGAGATATTCAAGGTGCGATTTCGAAAATCGATCCGGAGATTATCAAATCGCTGTTAGCTTCCACTGGAAACAATTCCGATTCATCGGCTAA
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270 280 290 300
AT5G13 ATCTCGTTCGGTTCCGTCTACTCCGAGGGAAGATCAGCCTCAGATCCCGATTTCTCCGGTCCACGCGTCTCTCGCCAGGTCTAGTACCGAACTCGTTTCG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_002 ATCTCGTTCGGTTCCGTCTACTCCGAGGGAAGATCAGCCTCAGATCCCGATTTCTCCGGTCCACGCGTCTCTCGCCAGGTCTAGTACCGAACTCGTTTCG
210 220 230 240 250 260 270 280 290 300
310 320 330 340 350 360 370 380 390 400
AT5G13 GGAACTGTTCCTATGACGATTTTCTACAATGGAAGTGTTTCAGTTTTCCAAGTGTCTCGTAACAAAGCTGGTGAAATTATGAAGGTCGCTAATGAAGCAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_002 GGAACTGTTCCTATGACGATTTTCTACAATGGAAGTGTTTCAGTTTTCCAAGTGTCTCGTAACAAAGCTGGTGAAATTATGAAGGTCGCTAATGAAGCAG
310 320 330 340 350 360 370 380 390 400
410 420 430 440 450 460 470 480 490 500
AT5G13 CATCTAAGAAAGACGAGTCGTCGATGGAGACAGATCTTTCGGTAATTCTTCCGACCACTCTAAGACCAAAGCTCTTTGGCCAGAATCTAGAAGGAGATCT
:::::::::::::::::::::::::::::::::::::::: :::::
IE_002 CATCTAAGAAAGACGAGTCGTCGATGGAGACAGATCTTTC-------------------------------------------------------GATCT
410 420 430 440
510 520 530 540 550 560 570 580 590
AT5G13 TCCCATCGCAAGGAGAAAGTCACTGCAACGTTTTCTCGAGAAGCGCAAGGAGAGATTAGTATCAACATCTCCTTACTATCCGACATCGGCC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_002 TCCCATCGCAAGGAGAAAGTCACTGCAACGTTTTCTCGAGAAGCGCAAGGAGAGAT-----------------------------------
450 460 470 480 490 500
10 20 30 40 50 60 70 80 90 100
AT5G13 MSKATIELDFLGLEKKQTNNAPKPKFQKFLDRRRSFRDIQGAISKIDPEIIKSLLASTGNNSDSSAKSRSVPSTPREDQPQIPISPVHASLARSSTELVS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_002 MSKATIELDFLGLEKKQTNNAPKPKFQKFLDRRRSFRDIQGAISKIDPEIIKSLLASTGNNSDSSAKSRSVPSTPREDQPQIPISPVHASLARSSTELVS
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190
AT5G13 GTVPMTIFYNGSVSVFQVSRNKAGEIMKVANEAASKKDESSMETDLSVILPT--TLRPKLFGQNLEGDLPIARRKSLQRFLEKRKERLVSTSPYYPTSA
::::::::::::::::::::::::::::::::::::::::::::::: : :. .: .. :
IE_002 GTVPMTIFYNGSVSVFQVSRNKAGEIMKVANEAASKKDESSMETDLS-IFPSQGESHCNVFSRSARRD-------------------------------
110 120 130 140 150 160
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