| Code name | IE_0044 | |||
|---|---|---|---|---|
| Locus | AT2G01200 | |||
| Template | IT_0044 (total cDNA) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | 8410 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGGACCCAAACACACCTGCAGAC) | |||
| Reverse primer | 8411 (GGGGACCACTTTGTACAAGAAAGCTGGGTCAAAGGGAAGAAGAGCATCGTTTCTT) | |||
| Alignment with template seq. | 1st | - | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列corresponding:kigoshi一致 | - | ||
| comment | AT2G01200.2, Splicing variant: IE_0045 | |||
| sequence |
>IE_0044_573bp ATGGACCCAAACACACCTGCAGACTTCTTCAAAGGTTCTTCCAAGTTTCATACATATTACTCACAGACCAAAAAGGGTGGTGGGGTAATCGATCTAGGCC TCAGCCTTAGGACCATACAACATGAAACTTACCTCCCACCGGCGCGAATGATAGGTCTCGACGGGTATGGAGAGCTCATAGACTGGTCGCAGCCCAGCTA TAACAGCATTACACAGTTGAAGAGTGAGGACACTGGACACCAAAGACTTGCCCAAGGATATTACAATAATGAAGGAGAGAGCAGAGGAAAATATGCTTAC GTAAAGGTAAATCTGGATGGCCTAGTGGTAGGGCGCAAGGTTTGCCTTGTTGATCAAGGAGCTTACGCAACTCTTGCTCTTCAGCTCAATGATATGTTTG GGATGCAGACCGTGTCGGGATTGAGGTTGTTCCAGACTGAGTCTGAGTTCTCTTTGGTCTACAGAGACAGAGAAGGCATTTGGAGGAATGTTGGGGATGT TCCATGGAAGGAGTTTGTCGAAAGCGTGGATCGGATGCGAATCGCAAGAAGAAACGATGCTCTTCTTCCCTTT >IE_0044_translated MDPNTPADFFKGSSKFHTYYSQTKKGGGVIDLGLSLRTIQHETYLPPARMIGLDGYGELIDWSQPSYNSITQLKSEDTGHQRLAQGYYNNEGESRGKYAY VKVNLDGLVVGRKVCLVDQGAYATLALQLNDMFGMQTVSGLRLFQTESEFSLVYRDREGIWRNVGDVPWKEFVESVDRMRIARRNDALLPF |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 429/573 (74.9%)（アミノ酸配列で135/194 (69.6%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT2G01 ATGGACCCAAACACACCTGCAGACTTCTTCAAAGGTTCTTCCAAGTTTCATACATATTACTCACAGACCAAAAAGGGTGGTGGGGTAATCGATCTAGGCC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_004 ATGGACCCAAACACACCTGCAGACTTCTTCAAAGGTTCTTCCAAGTTTCATACATATTACTCACAGACCAAAAAGGGTGGTGGGGTAATCGATCTAGGCC
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT2G01 TCAGCCTTAGGACCATACAACATGAAACTTACCTCCCACCGGCGCGAATGATAGGTCTCGACGGGTATGGAGAGCTCATAGACTGGTCGCAGCCCAGCTA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_004 TCAGCCTTAGGACCATACAACATGAAACTTACCTCCCACCGGCGCGAATGATAGGTCTCGACGGGTATGGAGAGCTCATAGACTGGTCGCAGCCCAGCTA
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270 280 290 300
AT2G01 TAACAGCATTACACAGTTGAAGAGTGAGGACACTGGACACCAAAGACTTGCCCAAGGATATTACAATAATGAAGGAGAGAGCAGAGGAAAATATGCTTAC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_004 TAACAGCATTACACAGTTGAAGAGTGAGGACACTGGACACCAAAGACTTGCCCAAGGATATTACAATAATGAAGGAGAGAGCAGAGGAAAATATGCTTAC
210 220 230 240 250 260 270 280 290 300
310 320 330 340 350 360 370 380 390
AT2G01 GTAAAGGTAAATCTGGATGGCCTAGTGGTAGGGCGCAAGGTTTGCCTTGTTGATCAAGGAGCTTACGCAACTCTTGCTCTTCAGCTCAATGATATGTTT-
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_004 GTAAAGGTAAATCTGGATGGCCTAGTGGTAGGGCGCAAGGTTTGCCTTGTTGATCAAGGAGCTTACGCAACTCTTGCTCTTCAGCTCAATGATATGTTTG
310 320 330 340 350 360 370 380 390 400
400 410 420
AT2G01 -------GACCGTGTCGGGATTGAGGTTGTTCCAGAC---------------------------------------------------------------
::::::::::::::::::::::::::::::
IE_004 GGATGCAGACCGTGTCGGGATTGAGGTTGTTCCAGACTGAGTCTGAGTTCTCTTTGGTCTACAGAGACAGAGAAGGCATTTGGAGGAATGTTGGGGATGT
410 420 430 440 450 460 470 480 490 500
AT2G01 -------------------------------------------------------------------------
IE_004 TCCATGGAAGGAGTTTGTCGAAAGCGTGGATCGGATGCGAATCGCAAGAAGAAACGATGCTCTTCTTCCCTTT
510 520 530 540 550 560 570
10 20 30 40 50 60 70 80 90 100
AT2G01 MDPNTPADFFKGSSKFHTYYSQTKKGGGVIDLGLSLRTIQHETYLPPARMIGLDGYGELIDWSQPSYNSITQLKSEDTGHQRLAQGYYNNEGESRGKYAY
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_004 MDPNTPADFFKGSSKFHTYYSQTKKGGGVIDLGLSLRTIQHETYLPPARMIGLDGYGELIDWSQPSYNSITQLKSEDTGHQRLAQGYYNNEGESRGKYAY
10 20 30 40 50 60 70 80 90 100
110 120 130 140
AT2G01 VKVNLDGLVVGRKVCLVDQGAYATLALQLNDMFDRVGIEVVPD---------------------------------------------------
::::::::::::::::::::::::::::::::: :.. :
IE_004 VKVNLDGLVVGRKVCLVDQGAYATLALQLNDMF---GMQTVSGLRLFQTESEFSLVYRDREGIWRNVGDVPWKEFVESVDRMRIARRNDALLPF
110 120 130 140 150 160 170 180 190
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