◆◆◆IE_0049 detail◆◆◆

Code name	IE_0049
Locus	AT4G21550
Template	IT_0049 (total cDNA)
Donor Vector	pDONR207
Forward primer	8404 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGCTTTCCTCTTCATCCATGTCA)
Reverse primer	8405 (GGGGACCACTTTGTACAAGAAAGCTGGGTCCATGGAGCTTGTGGTGGTGGTGTCT)
Alignment with template seq.	1st	-	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列corresponding:kigoshi一致	2nd	-
comment
sequence	>IE_0049_2145bp ATGCTTTCCTCTTCATCCATGTCATCGTCTTCGTTATCTGCTAGATTTTGCTTCAACCACGAATGTTTTGAGTTCAAACTCGACCATTGCCGTCCTGGTT GGCGTCTCCGTTCTGGCGATTTCGTCGACCTCTGTGATCGTTGCGCCTCTGCGTATGAACAAGGAAAGTTTTGTGACGTATTTCACCAAAGGGCTTCTGG ATGGAGGTGTTGTGAGTCATGTGGAAAGCGAATTCATTGTGGATGTATTGCGTCTGCTTCTGCTTATACGTTAATGGATGCTGGAGGAATTGAGTGTTTG GCTTGCGCAAGAAAAAAGTTTGCTTTGGGACCCAATTTTTCGCCATCACCGTCCTTTCTTTTCCAGTCTCCAATTTCTGAGAAATTCAAGGACTTGTCGA TCAACTGGAGTTCTTCAACTAGATCAAATCAGATAAGCTACCAGCCTCCTAGTTGTTTAGATCCTTCAGTTCTGCAGTTTGATTTTCGTAATCGTGGAGG TAATAATGAATTTAGTCAACCTGCTTCTAAAGAAAGGGTCACTGCATGTACCATGGAGAAAAAAAGGGGAATGAATGACATGATAGGGAAATTAATGAGT GAAAACTCGAAACATTATAGAGTATCTCCTTTTCCAAATGTCAATGTATATCACCCACTGATCTCACTAAAGGAGGGTCCATGTGGGACACAGCTTGCGT TTCCTGTGCCAATTACAACACCAATTGAGAAAACTGGTCACTCTAGACTAGATGGAAGTAATTTATGGCACACACGTAATTCTTCTCCGCTAAGTCGCTT ACACAATGACTTGAATGGTGGAGCAGACTCGCCATTCGAAAGCAAAAGTCGTAATGTGATGGCTCATCTTGAGACGCCAGGAAAATATCAGGTGGTTCCA CGATTTTGGCCCAAGGTTTCATATAAAAACCAAGTTCTGCAGAATCAATCAAAAGAATCTGAATCCGTTGTCACTCCTCTCTTTGAGAAGATCCTGAGTG CAACTGATACTGGAAAGCGGCTGGTCCTGCCAAAGAAATATGCAGAGGCTTTCTTGCCCCAACTTTCCCACACCAAAGGTGTGCCTCTCACAGTACAGGA CCCAATGGGTAAAGAGTGGAGATTTCAGTTTCGCTTTTGGCCCAGCAGCAAAGGCAGAATATATGTTCTAGAGGGAGTAACTCCATTCATACAGACCTTG CAGTTGCAGGCTGGTGATACAGTCATATTCAGTCGATTAGATCCAGAAAGGAAGTTAATTTTGGGATTCAGAAAGGCTTCGATTACTCAATCGTCTGATC AGGCTGATCCGGCTGATATGCATTCACCGTTTGAAGTTAAGAAGTCTGCTTATATAACTAAAGAAACCCCAGGAGTAGAATGTTCCTCTGGTAAGAAGAA AAGCAGCATGATGATTACAAGAAGTAAACGTCAAAAAGTTGAAAAGGGAGACGACAACTTGCTGAAGCTAACATGGGAAGAAGCTCAAGGATTTCTCCTG CCTCCTCCCAACCTCACTCCATCTAGAGTTGTGATAGAGGATTACGAGTTTGAGGAGTATGAGGAGGCTCCAATCATCGGGAAGCCTACTGATGTTGCAG GTTTCAGATCAACGTGCACTGAAGTTGAAGGACTGTTGATATCACCAACAACCACAAAGCACCCACGACATAGAGATGGCTGTACTTGCATCATTTGCAT ACAATCCCCAAGTGGGATAGGTCCTAAACACGACCGATGCTGTTCTTGTGCAGTCTGCGACACTAACAAACGCCGTAGGCGTTCCCTGTTGCTACGGAGG GAAAAGAAGCAGATGGAGAAAGAAGACAATGCGCGCAAGTTGCTCGAGCAACTGAATTCTGATAATGGACTTCACCAATCTGCTAACAACAGTGAGAATC ATGAGCGTCATGCTTCGCCTTTGAAAGTTCAATTAGATTTGAACTTCAAACCAGAAAAGGACGAAGAATCTCTCCCTGGTTCTAACAAAACTACCAAGAG CGAGACTCTGCCTCATGATGACACAGTAAAGTCTAGCTTCACGTCACCAAGCTCATCAAGTGCTCATAGCCAAAATAACAAGGAAGATGAAGGAAAACTC AAGACAACCACAGAAATTGCAGACACCACCACCACAAGCTCCATG >IE_0049_translated MLSSSSMSSSSLSARFCFNHECFEFKLDHCRPGWRLRSGDFVDLCDRCASAYEQGKFCDVFHQRASGWRCCESCGKRIHCGCIASASAYTLMDAGGIECL ACARKKFALGPNFSPSPSFLFQSPISEKFKDLSINWSSSTRSNQISYQPPSCLDPSVLQFDFRNRGGNNEFSQPASKERVTACTMEKKRGMNDMIGKLMS ENSKHYRVSPFPNVNVYHPLISLKEGPCGTQLAFPVPITTPIEKTGHSRLDGSNLWHTRNSSPLSRLHNDLNGGADSPFESKSRNVMAHLETPGKYQVVP RFWPKVSYKNQVLQNQSKESESVVTPLFEKILSATDTGKRLVLPKKYAEAFLPQLSHTKGVPLTVQDPMGKEWRFQFRFWPSSKGRIYVLEGVTPFIQTL QLQAGDTVIFSRLDPERKLILGFRKASITQSSDQADPADMHSPFEVKKSAYITKETPGVECSSGKKKSSMMITRSKRQKVEKGDDNLLKLTWEEAQGFLL PPPNLTPSRVVIEDYEFEEYEEAPIIGKPTDVAGFRSTCTEVEGLLISPTTTKHPRHRDGCTCIICIQSPSGIGPKHDRCCSCAVCDTNKRRRRSLLLRR EKKQMEKEDNARKLLEQLNSDNGLHQSANNSENHERHASPLKVQLDLNFKPEKDEESLPGSNKTTKSETLPHDDTVKSSFTSPSSSSAHSQNNKEDEGKL KTTTEIADTTTTSSM
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1990/2297 (86.6%)（アミノ酸配列で649/763 (85.1%)）一致しました。 10 20 30 40 AT4G21 -------------------------------------ATG--AGATACTTTTGCTCTTACTAC-----TTCTTGATTGTAAAC-------ATGCATACAT :: ::: ::::::: :: :: :: :::: : :::: ::: : : IE_004 ATGCTTTCCTCTTCATCCATGTCATCGTCTTCGTTATCTGCTAGA---TTTTGCTTCAACCACGAATGTT-TTGAGTTCAAACTCGACCATTGCCGTCCT 10 20 30 40 50 60 70 80 90 50 60 70 80 90 100 110 120 AT4G21 G-----------TGTTCTG---------------TCTTTGATGGGTTTATTTTTGGAAAACATCTCTGCGTATGAACAAGGAAAGTTTTGTGACGTATTT : :::::: ::: :::: : ::: : ::::::::::::::::::::::::::::::::::::: IE_004 GGTTGGCGTCTCCGTTCTGGCGATTTCGTCGACCTCTGTGATCG--------TTG-----CGCCTCTGCGTATGAACAAGGAAAGTTTTGTGACGTATTT 100 110 120 130 140 150 160 170 180 130 140 150 160 170 180 190 200 210 220 AT4G21 CACCAAAGGGCTTCTGGATGGAGGTGTTGTGAGTCATGTGGAAAGCATTTCGTGCAGCGAATTCATTGTGGATGTATTGCGTCTGCTTCTGCTTATACGT ::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::: IE_004 CACCAAAGGGCTTCTGGATGGAGGTGTTGTGAGTCATGTGGAA------------AGCGAATTCATTGTGGATGTATTGCGTCTGCTTCTGCTTATACGT 190 200 210 220 230 240 250 260 270 230 240 250 260 270 280 AT4G21 TAATGGATGCTGGAGGAATTGAGTGTTTGGCTTGCGCAAGAAAAAAGTTTGCTTT---------------------------------------GTCTCC ::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::: IE_004 TAATGGATGCTGGAGGAATTGAGTGTTTGGCTTGCGCAAGAAAAAAGTTTGCTTTGGGACCCAATTTTTCGCCATCACCGTCCTTTCTTTTCCAGTCTCC 280 290 300 310 320 330 340 350 360 370 290 300 310 320 330 340 350 360 370 380 AT4G21 AATTTCTGAGAAATTCAAGGACTTGTCGATCAACTGGAGTTCTTCAACTAGATCAAATCAGATAAGCTACCAGCCTCCTAGTTGTTTAGATCCTTCAGTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 AATTTCTGAGAAATTCAAGGACTTGTCGATCAACTGGAGTTCTTCAACTAGATCAAATCAGATAAGCTACCAGCCTCCTAGTTGTTTAGATCCTTCAGTT 380 390 400 410 420 430 440 450 460 470 390 400 410 420 430 440 450 460 470 480 AT4G21 CTGCAGTTTGATTTTCGTAATCGTGGAGGTAATAATGAATTTAGTCAACCTGCTTCTAAAGAAAGGGTCACTGCATGTACCATGGAGAAAAAAAGGGGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 CTGCAGTTTGATTTTCGTAATCGTGGAGGTAATAATGAATTTAGTCAACCTGCTTCTAAAGAAAGGGTCACTGCATGTACCATGGAGAAAAAAAGGGGAA 480 490 500 510 520 530 540 550 560 570 490 500 510 520 530 540 550 560 570 580 AT4G21 TGAATGACATGATAGGGAAATTAATGAGTGAAAACTCGAAACATTATAGAGTATCTCCTTTTCCAAATGTCAATGTATATCACCCACTGATCTCACTAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 TGAATGACATGATAGGGAAATTAATGAGTGAAAACTCGAAACATTATAGAGTATCTCCTTTTCCAAATGTCAATGTATATCACCCACTGATCTCACTAAA 580 590 600 610 620 630 640 650 660 670 590 600 610 620 630 640 650 660 670 680 AT4G21 GGAGGGTCCATGTGGGACACAGCTTGCGTTTCCTGTGCCAATTACAACACCAATTGAGAAAACTGGTCACTCTAGACTAGATGGAAGTAATTTATGGCAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 GGAGGGTCCATGTGGGACACAGCTTGCGTTTCCTGTGCCAATTACAACACCAATTGAGAAAACTGGTCACTCTAGACTAGATGGAAGTAATTTATGGCAC 680 690 700 710 720 730 740 750 760 770 690 700 710 720 730 740 750 760 770 780 AT4G21 ACACGTAATTCTTCTCCGCTAAGTCGCTTACACAATGACTTGAATGGTGGAGCAGACTCGCCATTCGAAAGCAAAAGTCGTAATGTGATGGCTCATCTTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 ACACGTAATTCTTCTCCGCTAAGTCGCTTACACAATGACTTGAATGGTGGAGCAGACTCGCCATTCGAAAGCAAAAGTCGTAATGTGATGGCTCATCTTG 780 790 800 810 820 830 840 850 860 870 790 800 810 820 830 840 850 860 870 AT4G21 AGACGCCAGGAAAATATCAGGTGGTTCCACGATTTTGGCCCAAGGTTTCATATAAAAACCAAGTTCTGCAGAATCAATCAAAAGA----GTATCCTT--- ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: : :::: : IE_004 AGACGCCAGGAAAATATCAGGTGGTTCCACGATTTTGGCCCAAGGTTTCATATAAAAACCAAGTTCTGCAGAATCAATCAAAAGAATCTGAATCCGTTGT 880 890 900 910 920 930 940 950 960 970 880 890 900 910 920 930 940 950 960 970 AT4G21 --CTTCTCTTATAGATACTACATTGGAATATAACTTT---AAGATCCTGAGTGCAACTGATACTGGAAAGCGGCTGGTCCTGCCAAAGAAATATGCAGAG :: :::: :::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 CACTCCTCT------------------------CTTTGAGAAGATCCTGAGTGCAACTGATACTGGAAAGCGGCTGGTCCTGCCAAAGAAATATGCAGAG 980 990 1000 1010 1020 1030 1040 980 990 1000 1010 1020 1030 1040 1050 1060 1070 AT4G21 GCTTTCTTGCCCCAACTTTCCCACACCAAAGGTGTGCCTCTCACAGTACAGGACCCAATGGGTAAAGAGTGGAGATTTCAGTTTCGCTTTTGGCCCAGCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 GCTTTCTTGCCCCAACTTTCCCACACCAAAGGTGTGCCTCTCACAGTACAGGACCCAATGGGTAAAGAGTGGAGATTTCAGTTTCGCTTTTGGCCCAGCA 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 AT4G21 GCAAAGGCAGAATATATGTTCTAGAGGGAGTAACTCCATTCATACAGACCTTGCAGTTGCAGGCTGGTGATACAGTCATATTCAGTCGATTAGATCCAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 GCAAAGGCAGAATATATGTTCTAGAGGGAGTAACTCCATTCATACAGACCTTGCAGTTGCAGGCTGGTGATACAGTCATATTCAGTCGATTAGATCCAGA 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 AT4G21 AAGGAAGTTAATTTTGGGATTCAGAAAGGCTTCGATTACTCAATCGTCTGATCAGGCTGATCCGGCTGATATGCATTCACCGTTTGAAGTTAAGAAGTCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 AAGGAAGTTAATTTTGGGATTCAGAAAGGCTTCGATTACTCAATCGTCTGATCAGGCTGATCCGGCTGATATGCATTCACCGTTTGAAGTTAAGAAGTCT 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 AT4G21 GCTTATATAACTAAAGAAACCCCAGGAGTAGAATGTTCCTCTGGTAAGAAGAAAAGCAGCATGATGATTACAAGAAGTAAACGTCAAAAAGTTGAAAAGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 GCTTATATAACTAAAGAAACCCCAGGAGTAGAATGTTCCTCTGGTAAGAAGAAAAGCAGCATGATGATTACAAGAAGTAAACGTCAAAAAGTTGAAAAGG 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 AT4G21 GAGACGACAACTTGCTGAAGCTAACATGGGAAGAAGCTCAAGGATTTCTCCTGCCTCCTCCCAACCTCACTCCATCTAGAGTTGTGATAGAGGATTACGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 GAGACGACAACTTGCTGAAGCTAACATGGGAAGAAGCTCAAGGATTTCTCCTGCCTCCTCCCAACCTCACTCCATCTAGAGTTGTGATAGAGGATTACGA 1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1480 1490 1500 1510 1520 1530 1540 1550 1560 1570 AT4G21 GTTTGAGGAGTATGAGGATTTGGCTGTAGTTTCTCAAGTTTCTTCTGGAATACAAGATTATTTCATGATATTAAAAATAACCTGTTGTTCATATACACAT :::::::::::::::: IE_004 GTTTGAGGAGTATGAG------------------------------------------------------------------------------------ 1550 1560 1580 1590 1600 1610 1620 1630 1640 1650 1660 AT4G21 TGGGTGCTAGAAGTTGAGGCTCCAATCATCGGGAAGCCTACTGATGTTGCAG------GATCAACGTGCACTGAAGTTGAAGGACTGTTGATATCACCAA ::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::: IE_004 ---------------GAGGCTCCAATCATCGGGAAGCCTACTGATGTTGCAGGTTTCAGATCAACGTGCACTGAAGTTGAAGGACTGTTGATATCACCAA 1570 1580 1590 1600 1610 1620 1630 1640 1670 1680 1690 1700 1710 1720 1730 1740 1750 1760 AT4G21 CAACCACAAAGCACCCACGACATAGAGATGGCTGTACTTGCATCATTTGCATACAATCCCCAAGTGGGATAGGTCCTAAACACGACCGATGCTGTTCTTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 CAACCACAAAGCACCCACGACATAGAGATGGCTGTACTTGCATCATTTGCATACAATCCCCAAGTGGGATAGGTCCTAAACACGACCGATGCTGTTCTTG 1650 1660 1670 1680 1690 1700 1710 1720 1730 1740 1770 1780 1790 1800 1810 1820 1830 1840 1850 1860 AT4G21 TGCAGTCTGCGACACTAACAAACGCCGTAGGCGTTCCCTGTTGCTACGGAGGGAAAAGAAGCAGATGGAGAAAGAAGACAATGCGCGCAAGTTGCTCGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 TGCAGTCTGCGACACTAACAAACGCCGTAGGCGTTCCCTGTTGCTACGGAGGGAAAAGAAGCAGATGGAGAAAGAAGACAATGCGCGCAAGTTGCTCGAG 1750 1760 1770 1780 1790 1800 1810 1820 1830 1840 1870 1880 1890 1900 1910 1920 1930 1940 1950 1960 AT4G21 CAACTGAATTCTGATAATGGACTTCACCAATCTGCTAACAACAGTGAGAATCATGAGCGTCATGCTTCGCCTTTGAAAGTTCAATTAGATTTGAACTTCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 CAACTGAATTCTGATAATGGACTTCACCAATCTGCTAACAACAGTGAGAATCATGAGCGTCATGCTTCGCCTTTGAAAGTTCAATTAGATTTGAACTTCA 1850 1860 1870 1880 1890 1900 1910 1920 1930 1940 1970 1980 1990 2000 2010 2020 2030 2040 2050 2060 AT4G21 AACCAGAAAAGGACGAAGAATCTCTCCCTGGTTCTAACAAAACTACCAAGAGCGAGACTCTGCCTCATGATGACACAGTAAAGTCTAGCTTCACGTCACC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 AACCAGAAAAGGACGAAGAATCTCTCCCTGGTTCTAACAAAACTACCAAGAGCGAGACTCTGCCTCATGATGACACAGTAAAGTCTAGCTTCACGTCACC 1950 1960 1970 1980 1990 2000 2010 2020 2030 2040 2070 2080 2090 2100 2110 2120 2130 2140 2150 2160 AT4G21 AAGCTCATCAAGTGCTCATAGCCAAAATAACAAGGAAGATGAAGGAAAACTCAAGACAACCACAGAAATTGCAGACACCACCACCACAAGCTCCATG ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 AAGCTCATCAAGTGCTCATAGCCAAAATAACAAGGAAGATGAAGGAAAACTCAAGACAACCACAGAAATTGCAGACACCACCACCACAAGCTCCATG 2050 2060 2070 2080 2090 2100 2110 2120 2130 2140 10 20 30 40 50 60 70 AT4G21 ------------MRYFCSYYFLIVNMHTCVLSLM-----------GLFLE----NISAYEQGKFCDVFHQRASGWRCCESCGKHFVQRIHCGCIASASAY :: : : . : :.. ::::::::::::::::::::::::::: ::::::::::::: IE_004 MLSSSSMSSSSLSARFCF-------NHECFEFKLDHCRPGWRLRSGDFVDLCDRCASAYEQGKFCDVFHQRASGWRCCESCGK----RIHCGCIASASAY 10 20 30 40 50 60 70 80 80 90 100 110 120 130 140 150 160 AT4G21 TLMDAGGIECLACARKKFAL-------------SPISEKFKDLSINWSSSTRSNQISYQPPSCLDPSVLQFDFRNRGGNNEFSQPASKERVTACTMEKKR :::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 TLMDAGGIECLACARKKFALGPNFSPSPSFLFQSPISEKFKDLSINWSSSTRSNQISYQPPSCLDPSVLQFDFRNRGGNNEFSQPASKERVTACTMEKKR 90 100 110 120 130 140 150 160 170 180 170 180 190 200 210 220 230 240 250 260 AT4G21 GMNDMIGKLMSENSKHYRVSPFPNVNVYHPLISLKEGPCGTQLAFPVPITTPIEKTGHSRLDGSNLWHTRNSSPLSRLHNDLNGGADSPFESKSRNVMAH :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 GMNDMIGKLMSENSKHYRVSPFPNVNVYHPLISLKEGPCGTQLAFPVPITTPIEKTGHSRLDGSNLWHTRNSSPLSRLHNDLNGGADSPFESKSRNVMAH 190 200 210 220 230 240 250 260 270 280 270 280 290 300 310 320 330 340 350 360 AT4G21 LETPGKYQVVPRFWPKVSYKNQVLQNQSKEYPSSLIDTTLEYNFKILSATDTGKRLVLPKKYAEAFLPQLSHTKGVPLTVQDPMGKEWRFQFRFWPSSKG :::::::::::::::::::::::::::::: :.. : :::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 LETPGKYQVVPRFWPKVSYKNQVLQNQSKE-SESVVTPLFE---KILSATDTGKRLVLPKKYAEAFLPQLSHTKGVPLTVQDPMGKEWRFQFRFWPSSKG 290 300 310 320 330 340 350 360 370 380 370 380 390 400 410 420 430 440 450 460 AT4G21 RIYVLEGVTPFIQTLQLQAGDTVIFSRLDPERKLILGFRKASITQSSDQADPADMHSPFEVKKSAYITKETPGVECSSGKKKSSMMITRSKRQKVEKGDD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 RIYVLEGVTPFIQTLQLQAGDTVIFSRLDPERKLILGFRKASITQSSDQADPADMHSPFEVKKSAYITKETPGVECSSGKKKSSMMITRSKRQKVEKGDD 390 400 410 420 430 440 450 460 470 480 470 480 490 500 510 520 530 540 550 AT4G21 NLLKLTWEEAQGFLLPPPNLTPSRVVIEDYEFEEYEDLAVVSQVSSGIQDYFMILKITCCSYTHWVLEVEAPIIGKPTDVAG--STCTEVEGLLISPTTT :::::::::::::::::::::::::::::::::::: ::::::::::::: :::::::::::::::: IE_004 NLLKLTWEEAQGFLLPPPNLTPSRVVIEDYEFEEYE---------------------------------EAPIIGKPTDVAGFRSTCTEVEGLLISPTTT 490 500 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 AT4G21 KHPRHRDGCTCIICIQSPSGIGPKHDRCCSCAVCDTNKRRRRSLLLRREKKQMEKEDNARKLLEQLNSDNGLHQSANNSENHERHASPLKVQLDLNFKPE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 KHPRHRDGCTCIICIQSPSGIGPKHDRCCSCAVCDTNKRRRRSLLLRREKKQMEKEDNARKLLEQLNSDNGLHQSANNSENHERHASPLKVQLDLNFKPE 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 710 720 AT4G21 KDEESLPGSNKTTKSETLPHDDTVKSSFTSPSSSSAHSQNNKEDEGKLKTTTEIADTTTTSSM ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_004 KDEESLPGSNKTTKSETLPHDDTVKSSFTSPSSSSAHSQNNKEDEGKLKTTTEIADTTTTSSM 660 670 680 690 700 710

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