| Code name | IE_0054 | |||
|---|---|---|---|---|
| Locus | AT1G25250 | |||
| Template | IT_0054 (total cDNA) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | 8440 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGGAGCTGACGCAACCCATAAGA) | |||
| Reverse primer | 8441 (GGGGACCACTTTGTACAAGAAAGCTGGGTCCTCGCATTCTCCTTCAGTTGTCGCC) | |||
| Alignment with template seq. | 1st | - | 2nd | - |
| Alignment with TAIR7CDS | ã¢ããé ¸é åcorresponding:kigoshiä¸è´ | - | ||
| comment | スタート位置をずらした。 | |||
| sequence |
>IE_0054_1155bp ATGATACATTACGAACAAAACAACAACCTTCAGAACCTTCCATCTTCATCTTCCAACGATCTTCTTCTTGGCATCAATGGAGCTGACGCAACCCATAAGA GAAAACGGAGACCCGCAGGGACACCAGATCCAGATGCAGAGGTGGTATCTTTGTCACCAAGAACGTTACTAGAATCAGATCGGTACGTGTGTGAGATCTG CAACCAAGGGTTTCAGAGAGATCAGAATCTACAGATGCATAGAAGACGACATAAAGTGCCATGGAAGCTTCTGAAGAGAGACAAGAAGGATGAGGAAGTG AGGAAGAGAGTCTACGTGTGTCCTGAGCCAACATGTCTCCACCATGACCCGTGCCACGCTCTTGGAGATCTGGTCGGAATCAAAAAGCACTTCCGCCGTA AACACAGTGTCCACAAGCAATGGGTTTGCGAGAGATGCTCCAAAGGCTATGCAGTTCAATCTGACTACAAAGCCCATCTCAAGACCTGCGGTTCACGCGG TCACTCCTGCGACTGTGGCCGCGTTTTCTCCAGGGTTGAGAGTTTCATAGAGCATCAAGATACTTGCACCATCCGGCAACCTCAACCCACCAACCACCGT CATCTACAGCAGCACACGATGGGCCTTGACGCTCCATCAAGAACCACTTCGACAGCAAGCTTCGGCCCTTTATTGCATGGACTTCCTTTGTTAAGACCTC CACGACCGTCCAATCAACATTCACCAGCTTTTGCTTACCCTTTCAATGCTTCATCAGCTCCTTTCGAAAGCCTCGAACTTCAACTATCCATAGGAATGGC AAGAACATCAGCACAAGCGAGGCACAACGAAAAGAGAGAGACGAGTTTAACAAAGGAGAGAGCGAACGAGGAGGCAAGGAAGGCAGAGGAAACGAGACAA GAAGCGAAAAGGCAGATAGAGATGGCAGAAAAGGATTTTGAGAAAGCTAAGAGAATAAGGGAAGAAGCAAAAACCGAGTTGGAGAAGGCTCACGTTGTTA GAGAAGAAGCAATCAAGAGGATTAATGCAACAATGATGGAGATCACTTGCCACTCTTGCAAGCAACTATTTCAGCTGCCTGTTACGGCTGATGAGAGTAC GTCGTCTCTTGTTATGAGTTACGTTTCCTCGGCGACAACTGAAGGAGAATGCGAG >IE_0054_translated MIHYEQNNNLQNLPSSSSNDLLLGINGADATHKRKRRPAGTPDPDAEVVSLSPRTLLESDRYVCEICNQGFQRDQNLQMHRRRHKVPWKLLKRDKKDEEV RKRVYVCPEPTCLHHDPCHALGDLVGIKKHFRRKHSVHKQWVCERCSKGYAVQSDYKAHLKTCGSRGHSCDCGRVFSRVESFIEHQDTCTIRQPQPTNHR HLQQHTMGLDAPSRTTSTASFGPLLHGLPLLRPPRPSNQHSPAFAYPFNASSAPFESLELQLSIGMARTSAQARHNEKRETSLTKERANEEARKAEETRQ EAKRQIEMAEKDFEKAKRIREEAKTELEKAHVVREEAIKRINATMMEITCHSCKQLFQLPVTADESTSSLVMSYVSSATTEGECE |
|||
| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1079/1162 (92.9%)（アミノ酸配列で347/391 (88.7%)）一致しました。
10 20
AT1G25 ----------------------------------------------------------------------------ATGGAGCTGACGCAACCCATAAGA
::::::::::::::::::::::::
IE_005 ATGATACATTACGAACAAAACAACAACCTTCAGAACCTTCCATCTTCATCTTCCAACGATCTTCTTCTTGGCATCAATGGAGCTGACGCAACCCATAAGA
10 20 30 40 50 60 70 80 90 100
30 40 50 60 70 80 90 100 110 120
AT1G25 GAAAACGGAGACCCGCAGGGACACCAGCTAACAGATCCAGATGCAGAGGTGGTATCTTTGTCACCAAGAACGTTACTAGAATCAGATCGGTACGTGTGTG
:::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 GAAAACGGAGACCCGCAGGGACAC-------CAGATCCAGATGCAGAGGTGGTATCTTTGTCACCAAGAACGTTACTAGAATCAGATCGGTACGTGTGTG
110 120 130 140 150 160 170 180 190
130 140 150 160 170 180 190 200 210 220
AT1G25 AGATCTGCAACCAAGGGTTTCAGAGAGATCAGAATCTACAGATGCATAGAAGACGACATAAAGTGCCATGGAAGCTTCTGAAGAGAGACAAGAAGGATGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 AGATCTGCAACCAAGGGTTTCAGAGAGATCAGAATCTACAGATGCATAGAAGACGACATAAAGTGCCATGGAAGCTTCTGAAGAGAGACAAGAAGGATGA
200 210 220 230 240 250 260 270 280 290
230 240 250 260 270 280 290 300 310 320
AT1G25 GGAAGTGAGGAAGAGAGTCTACGTGTGTCCTGAGCCAACATGTCTCCACCATGACCCGTGCCACGCTCTTGGAGATCTGGTCGGAATCAAAAAGCACTTC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 GGAAGTGAGGAAGAGAGTCTACGTGTGTCCTGAGCCAACATGTCTCCACCATGACCCGTGCCACGCTCTTGGAGATCTGGTCGGAATCAAAAAGCACTTC
300 310 320 330 340 350 360 370 380 390
330 340 350 360 370 380 390 400 410 420
AT1G25 CGCCGTAAACACAGTGTCCACAAGCAATGGGTTTGCGAGAGATGCTCCAAAGGCTATGCAGTTCAATCTGACTACAAAGCCCATCTCAAGACCTGCGGTT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 CGCCGTAAACACAGTGTCCACAAGCAATGGGTTTGCGAGAGATGCTCCAAAGGCTATGCAGTTCAATCTGACTACAAAGCCCATCTCAAGACCTGCGGTT
400 410 420 430 440 450 460 470 480 490
430 440 450 460 470 480 490 500 510 520
AT1G25 CACGCGGTCACTCCTGCGACTGTGGCCGCGTTTTCTCCAGGGTTGAGAGTTTCATAGAGCATCAAGATACTTGCACCATCCGGCAACCTCAACCCACCAA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 CACGCGGTCACTCCTGCGACTGTGGCCGCGTTTTCTCCAGGGTTGAGAGTTTCATAGAGCATCAAGATACTTGCACCATCCGGCAACCTCAACCCACCAA
500 510 520 530 540 550 560 570 580 590
530 540 550 560 570 580 590 600 610 620
AT1G25 CCACCGTCATCTACAGCAGCACACGATGGGCCTTGACGCTCCATCAAGAACCACTTCGACAGCAAGCTTCGGCCCTTTATTGCATGGACTTCCTTTGTTA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 CCACCGTCATCTACAGCAGCACACGATGGGCCTTGACGCTCCATCAAGAACCACTTCGACAGCAAGCTTCGGCCCTTTATTGCATGGACTTCCTTTGTTA
600 610 620 630 640 650 660 670 680 690
630 640 650 660 670 680 690 700 710 720
AT1G25 AGACCTCCACGACCGTCCAATCAACATTCACCAGCTTTTGCTTACCCTTTCAATGCTTCATCAGCTCCTTTCGAAAGCCTCGAACTTCAACTATCCATAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 AGACCTCCACGACCGTCCAATCAACATTCACCAGCTTTTGCTTACCCTTTCAATGCTTCATCAGCTCCTTTCGAAAGCCTCGAACTTCAACTATCCATAG
700 710 720 730 740 750 760 770 780 790
730 740 750 760 770 780 790 800 810 820
AT1G25 GAATGGCAAGAACATCAGCACAAGCGAGGCACAACGAAAAGAGAGAGACGAGTTTAACAAAGGAGAGAGCGAACGAGGAGGCAAGGAAGGCAGAGGAAAC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 GAATGGCAAGAACATCAGCACAAGCGAGGCACAACGAAAAGAGAGAGACGAGTTTAACAAAGGAGAGAGCGAACGAGGAGGCAAGGAAGGCAGAGGAAAC
800 810 820 830 840 850 860 870 880 890
830 840 850 860 870 880 890 900 910 920
AT1G25 GAGACAAGAAGCGAAAAGGCAGATAGAGATGGCAGAAAAGGATTTTGAGAAAGCTAAGAGAATAAGGGAAGAAGCAAAAACCGAGTTGGAGAAGGCTCAC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 GAGACAAGAAGCGAAAAGGCAGATAGAGATGGCAGAAAAGGATTTTGAGAAAGCTAAGAGAATAAGGGAAGAAGCAAAAACCGAGTTGGAGAAGGCTCAC
900 910 920 930 940 950 960 970 980 990
930 940 950 960 970 980 990 1000 1010 1020
AT1G25 GTTGTTAGAGAAGAAGCAATCAAGAGGATTAATGCAACAATGATGGAGATCACTTGCCACTCTTGCAAGCAACTATTTCAGCTGCCTGTTACGGCTGATG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 GTTGTTAGAGAAGAAGCAATCAAGAGGATTAATGCAACAATGATGGAGATCACTTGCCACTCTTGCAAGCAACTATTTCAGCTGCCTGTTACGGCTGATG
1000 1010 1020 1030 1040 1050 1060 1070 1080 1090
1030 1040 1050 1060 1070 1080
AT1G25 AGAGTACGTCGTCTCTTGTTATGAGTTACGTTTCCTCGGCGACAACTGAAGGAGAATGCGAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 AGAGTACGTCGTCTCTTGTTATGAGTTACGTTTCCTCGGCGACAACTGAAGGAGAATGCGAG
1100 1110 1120 1130 1140 1150
10 20 30 40 50 60 70
AT1G25 ---------------------------MELT--QPIRENGDPQGHQLTDPDAEVVSLSPRTLLESDRYVCEICNQGFQRDQNLQMHRRRHKVPWKLLKRD
. : . : : : ::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 MIHYEQNNNLQNLPSSSSNDLLLGINGADATHKRKRRPAGTP------DPDAEVVSLSPRTLLESDRYVCEICNQGFQRDQNLQMHRRRHKVPWKLLKRD
10 20 30 40 50 60 70 80 90
80 90 100 110 120 130 140 150 160 170
AT1G25 KKDEEVRKRVYVCPEPTCLHHDPCHALGDLVGIKKHFRRKHSVHKQWVCERCSKGYAVQSDYKAHLKTCGSRGHSCDCGRVFSRVESFIEHQDTCTIRQP
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 KKDEEVRKRVYVCPEPTCLHHDPCHALGDLVGIKKHFRRKHSVHKQWVCERCSKGYAVQSDYKAHLKTCGSRGHSCDCGRVFSRVESFIEHQDTCTIRQP
100 110 120 130 140 150 160 170 180 190
180 190 200 210 220 230 240 250 260 270
AT1G25 QPTNHRHLQQHTMGLDAPSRTTSTASFGPLLHGLPLLRPPRPSNQHSPAFAYPFNASSAPFESLELQLSIGMARTSAQARHNEKRETSLTKERANEEARK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 QPTNHRHLQQHTMGLDAPSRTTSTASFGPLLHGLPLLRPPRPSNQHSPAFAYPFNASSAPFESLELQLSIGMARTSAQARHNEKRETSLTKERANEEARK
200 210 220 230 240 250 260 270 280 290
280 290 300 310 320 330 340 350 360
AT1G25 AEETRQEAKRQIEMAEKDFEKAKRIREEAKTELEKAHVVREEAIKRINATMMEITCHSCKQLFQLPVTADESTSSLVMSYVSSATTEGECE
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_005 AEETRQEAKRQIEMAEKDFEKAKRIREEAKTELEKAHVVREEAIKRINATMMEITCHSCKQLFQLPVTADESTSSLVMSYVSSATTEGECE
300 310 320 330 340 350 360 370 380
|
|||