◆◆◆IE_0057 detail◆◆◆

Code name	IE_0057
Locus	AT4G32010
Template	IT_0057 (total cDNA)
Donor Vector	pDONR207
Forward primer	8372 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGGAGTCAATAAAGGTTTGCATG)
Reverse primer	8373 (GGGGACCACTTTGTACAAGAAAGCTGGGTCGTTCACAGGATCATGAGCTCCATTT)
Alignment with template seq.	1st	-	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列corresponding:kigoshi一致	2nd	-
comment
sequence	>IE_0057_2328bp ATGGAGTCAATAAAGGTTTGCATGAACGCACTGTGCGGAGCGGCCTCTACGTCGGGCGAGTGGAAAAAAGGCTGGCCTATGCGATCCGGTGATTTAGCTT CTCTCTGCGATAAGTGTGGGTGTGCATACGAGCAATCCATATTCTGTGAAGTGTTCCATGCCAAGGAATCTGGTTGGAGAGAGTGTAATTCATGTGACAA GCGTCTTCACTGTGGATGCATTGCTTCTAGATTTATGATGGAGCTTCTAGAGAATGGTGGTGTTACCTGTATAAGTTGCGCCAAGAAATCCGGACTAATT TCTATGAATGTGAGCCATGAATCTAACGGTAAGGACTTCCCCTCATTTGCTTCAGCAGAGCATGTAGGCAGTGTTCTTGAGAGGACAAATCTCAAGCACT TGCTTCACTTTCAAAGAATCGACCCCACTCATTCTTCTCTTCAAATGAAACAAGAAGAATCGCTGCTTCCTTCCAGCCTAGATGCTCTTAGACACAAAAC TGAAAGGAAAGAATTGTCTGCACAGCCAAACTTGAGCATTTCACTTGGACCTACGCTTATGACAAGCCCATTTCATGATGCTGCTGTTGATGACAGAAGT AAGACTAATTCGATTTTCCAACTGGCCCCTCGGTCCAGGCAGCTGCTTCCAAAACCTGCAAATTCAGCTCCCATTGCTGCTGGCATGGAGCCTAGTGGGA GCCTGGTGTCACAGATTCATGTCGCTCGGCCTCCTCCAGAAGGTCGCGGGAAGACCCAATTGCTTCCCCGTTACTGGCCTAGGATTACTGACCAAGAGCT GCTGCAATTATCTGGACACTCAAATTCCAAAATTATACCACTCTTTGAAAAAGTTCTGAGTGCGAGCGATGCGGGTCGTATTGGTCGACTGGTTCTTCCG AAAGCATGTGCAGAGGCATATTTCCCCCCTATATCTCTACCCGAGGGTCTCCCGTTAAAGATACAAGACATAAAAGGGAAAGAATGGGTGTTCCAGTTCA GGTTTTGGCCTAATAATAACAGCAGGATGTACGTTTTGGAGGGTGTGACTCCTTGCATACAGTCCATGCAGTTGCAAGCTGGTGACACTGTAACATTCAG CCGTACAGAACCTGAAGGAAAACTCGTAATGGGATACCGTAAAGCGACGAACTCTACAGCGACACAGATGTTCAAGGGAAGCAGTGAACCCAATCTGAAC ATGTTTTCCAACAGCTTGAATCCGGGATGTGGTGACATCAATTGGTCTAAACTAGAGAAGTCTGAGGACATGGCAAAGGATAACTTATTTCTTCAGTCGT CCTTAACTTCTGCTAGGAAACGGGTTCGGAACATTGGGACTAAGAGCAAGCGTCTGCTCATTGATAGCGTAGATGTTCTGGAACTGAAAATAACTTGGGA GGAGGCACAGGAGCTGTTGCGGCCTCCCCAATCCACCAAACCCAGCATCTTTACGCTGGAAAATCAAGATTTTGAAGAATATGACGAACCACCAGTTTTC GGGAAGAGGACCCTTTTTGTCTCACGTCAAACAGGGGAACAAGAGCAATGGGTGCAGTGTGATGCTTGTGGGAAATGGCGACAGCTGCCGGTGGATATTC TTCTTCCACCAAAGTGGTCGTGCTCTGATAATCTCTTGGATCCTGGCAGGTCTTCATGTTCCGCACCTGATGAACTCTCTCCAAGAGAACAGGATACACT TGTCCGGCAGAGCAAAGAGTTCAAAAGGAGGAGACTGGCATCATCAAACGAAAAGCTAAACCAGTCGCAGGATGCATCTGCTCTGAATAGTTTAGGAAAT GCAGGCATCACCACAACCGGTGAACAGGGGGAAATCACGGTTGCAGCCACGACCAAGCATCCAAGACACCGGGCAGGGTGTTCGTGCATCGTCTGCAGCC AACCACCGAGCGGAAAAGGCAAACACAAGCCGTCATGCACTTGCACTGTGTGCGAGGCAGTGAAGAGACGATTCAGGACGCTCATGCTGCGGAAGCGGAA CAAAGGAGAGGCAGGACAGGCAAGCCAGCAGGCGCAGTCACAGTCAGAGTGCAGGGACGAGACAGAAGTGGAGAGCATTCCAGCGGTTGAACTAGCCGCA GGGGAAAACATCGACTTGAACTCAGACCCGGGGGCTTCCCGAGTAAGCATGATGAGGCTTCTCCAAGCTGCAGCGTTTCCTCTGGAAGCATATCTGAAAC AAAAGGCTATTTCCAATACAGCAGGAGAACAGCAAAGCAGTGATATGGTCAGCACAGAACACGGTTCGTCCTCAGCCGCACAAGAAACTGAGAAAGACAC AACAAATGGAGCTCATGATCCTGTGAAC >IE_0057_translated MESIKVCMNALCGAASTSGEWKKGWPMRSGDLASLCDKCGCAYEQSIFCEVFHAKESGWRECNSCDKRLHCGCIASRFMMELLENGGVTCISCAKKSGLI SMNVSHESNGKDFPSFASAEHVGSVLERTNLKHLLHFQRIDPTHSSLQMKQEESLLPSSLDALRHKTERKELSAQPNLSISLGPTLMTSPFHDAAVDDRS KTNSIFQLAPRSRQLLPKPANSAPIAAGMEPSGSLVSQIHVARPPPEGRGKTQLLPRYWPRITDQELLQLSGHSNSKIIPLFEKVLSASDAGRIGRLVLP KACAEAYFPPISLPEGLPLKIQDIKGKEWVFQFRFWPNNNSRMYVLEGVTPCIQSMQLQAGDTVTFSRTEPEGKLVMGYRKATNSTATQMFKGSSEPNLN MFSNSLNPGCGDINWSKLEKSEDMAKDNLFLQSSLTSARKRVRNIGTKSKRLLIDSVDVLELKITWEEAQELLRPPQSTKPSIFTLENQDFEEYDEPPVF GKRTLFVSRQTGEQEQWVQCDACGKWRQLPVDILLPPKWSCSDNLLDPGRSSCSAPDELSPREQDTLVRQSKEFKRRRLASSNEKLNQSQDASALNSLGN AGITTTGEQGEITVAATTKHPRHRAGCSCIVCSQPPSGKGKHKPSCTCTVCEAVKRRFRTLMLRKRNKGEAGQASQQAQSQSECRDETEVESIPAVELAA GENIDLNSDPGASRVSMMRLLQAAAFPLEAYLKQKAISNTAGEQQSSDMVSTEHGSSSAAQETEKDTTNGAHDPVN
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 2328/2340 (99.5%)（アミノ酸配列で775/780 (99.4%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G32 ATGGAGTCAATAAAGGTTTGCATGAACGCACTGTGCGGAGCGGCCTCTACGTCGGGCGAGTGGAAAAAAGGCTGGCCTATGCGATCCGGTGATTTAGCTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 ATGGAGTCAATAAAGGTTTGCATGAACGCACTGTGCGGAGCGGCCTCTACGTCGGGCGAGTGGAAAAAAGGCTGGCCTATGCGATCCGGTGATTTAGCTT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G32 CTCTCTGCGATAAGTGTGGGTGTGCATACGAGCAATCCATATTCTGTGAAGTGTTCCATGCCAAGGAATCTGGTTGGAGAGAGTGTAATTCATGTGACAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 CTCTCTGCGATAAGTGTGGGTGTGCATACGAGCAATCCATATTCTGTGAAGTGTTCCATGCCAAGGAATCTGGTTGGAGAGAGTGTAATTCATGTGACAA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G32 GCGTCTTCACTGTGGATGCATTGCTTCTAGATTTATGATGGAGCTTCTAGAGAATGGTGGTGTTACCTGTATAAGTTGCGCCAAGAAATCCGGACTAATT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 GCGTCTTCACTGTGGATGCATTGCTTCTAGATTTATGATGGAGCTTCTAGAGAATGGTGGTGTTACCTGTATAAGTTGCGCCAAGAAATCCGGACTAATT 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT4G32 TCTATGAATGTGAGCCATGAATCTAACGGTAAGGACTTCCCCTCATTTGCTTCAGCAGAGCATGTAGGCAGTGTTCTTGAGAGGACAAATCTCAAGCACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 TCTATGAATGTGAGCCATGAATCTAACGGTAAGGACTTCCCCTCATTTGCTTCAGCAGAGCATGTAGGCAGTGTTCTTGAGAGGACAAATCTCAAGCACT 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT4G32 TGCTTCACTTTCAAAGAATCGACCCCACTCATTCTTCTCTTCAAATGAAACAAGAAGAATCGCTGCTTCCTTCCAGCCTAGATGCTCTTAGACACAAAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 TGCTTCACTTTCAAAGAATCGACCCCACTCATTCTTCTCTTCAAATGAAACAAGAAGAATCGCTGCTTCCTTCCAGCCTAGATGCTCTTAGACACAAAAC 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT4G32 TGAAAGGAAAGAATTGTCTGCACAGCCAAACTTGAGCATTTCACTTGGACCTACGCTTATGACAAGCCCATTTCATGATGCTGCTGTTGATGACAGAAGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 TGAAAGGAAAGAATTGTCTGCACAGCCAAACTTGAGCATTTCACTTGGACCTACGCTTATGACAAGCCCATTTCATGATGCTGCTGTTGATGACAGAAGT 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT4G32 AAGACTAATTCGATTTTCCAACTGGCCCCTCGGTCCAGGCAGCTGCTTCCAAAACCTGCAAATTCAGCTCCCATTGCTGCTGGCATGGAGCCTAGTGGGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 AAGACTAATTCGATTTTCCAACTGGCCCCTCGGTCCAGGCAGCTGCTTCCAAAACCTGCAAATTCAGCTCCCATTGCTGCTGGCATGGAGCCTAGTGGGA 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT4G32 GCCTGGTGTCACAGATTCATGTCGCTCGGCCTCCTCCAGAAGGTCGCGGGAAGACCCAATTGCTTCCCCGTTACTGGCCTAGGATTACTGACCAAGAGCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 GCCTGGTGTCACAGATTCATGTCGCTCGGCCTCCTCCAGAAGGTCGCGGGAAGACCCAATTGCTTCCCCGTTACTGGCCTAGGATTACTGACCAAGAGCT 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT4G32 GCTGCAATTATCTGGACAGTATCCTCATCTCTCAAATTCCAAAATTATACCACTCTTTGAAAAAGTTCTGAGTGCGAGCGATGCGGGTCGTATTGGTCGA :::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 GCTGCAATTATCTGGACA------------CTCAAATTCCAAAATTATACCACTCTTTGAAAAAGTTCTGAGTGCGAGCGATGCGGGTCGTATTGGTCGA 810 820 830 840 850 860 870 880 910 920 930 940 950 960 970 980 990 1000 AT4G32 CTGGTTCTTCCGAAAGCATGTGCAGAGGCATATTTCCCCCCTATATCTCTACCCGAGGGTCTCCCGTTAAAGATACAAGACATAAAAGGGAAAGAATGGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 CTGGTTCTTCCGAAAGCATGTGCAGAGGCATATTTCCCCCCTATATCTCTACCCGAGGGTCTCCCGTTAAAGATACAAGACATAAAAGGGAAAGAATGGG 890 900 910 920 930 940 950 960 970 980 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT4G32 TGTTCCAGTTCAGGTTTTGGCCTAATAATAACAGCAGGATGTACGTTTTGGAGGGTGTGACTCCTTGCATACAGTCCATGCAGTTGCAAGCTGGTGACAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 TGTTCCAGTTCAGGTTTTGGCCTAATAATAACAGCAGGATGTACGTTTTGGAGGGTGTGACTCCTTGCATACAGTCCATGCAGTTGCAAGCTGGTGACAC 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT4G32 TGTAACATTCAGCCGTACAGAACCTGAAGGAAAACTCGTAATGGGATACCGTAAAGCGACGAACTCTACAGCGACACAGATGTTCAAGGGAAGCAGTGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 TGTAACATTCAGCCGTACAGAACCTGAAGGAAAACTCGTAATGGGATACCGTAAAGCGACGAACTCTACAGCGACACAGATGTTCAAGGGAAGCAGTGAA 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT4G32 CCCAATCTGAACATGTTTTCCAACAGCTTGAATCCGGGATGTGGTGACATCAATTGGTCTAAACTAGAGAAGTCTGAGGACATGGCAAAGGATAACTTAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 CCCAATCTGAACATGTTTTCCAACAGCTTGAATCCGGGATGTGGTGACATCAATTGGTCTAAACTAGAGAAGTCTGAGGACATGGCAAAGGATAACTTAT 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT4G32 TTCTTCAGTCGTCCTTAACTTCTGCTAGGAAACGGGTTCGGAACATTGGGACTAAGAGCAAGCGTCTGCTCATTGATAGCGTAGATGTTCTGGAACTGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 TTCTTCAGTCGTCCTTAACTTCTGCTAGGAAACGGGTTCGGAACATTGGGACTAAGAGCAAGCGTCTGCTCATTGATAGCGTAGATGTTCTGGAACTGAA 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT4G32 AATAACTTGGGAGGAGGCACAGGAGCTGTTGCGGCCTCCCCAATCCACCAAACCCAGCATCTTTACGCTGGAAAATCAAGATTTTGAAGAATATGACGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 AATAACTTGGGAGGAGGCACAGGAGCTGTTGCGGCCTCCCCAATCCACCAAACCCAGCATCTTTACGCTGGAAAATCAAGATTTTGAAGAATATGACGAA 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 AT4G32 CCACCAGTTTTCGGGAAGAGGACCCTTTTTGTCTCACGTCAAACAGGGGAACAAGAGCAATGGGTGCAGTGTGATGCTTGTGGGAAATGGCGACAGCTGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 CCACCAGTTTTCGGGAAGAGGACCCTTTTTGTCTCACGTCAAACAGGGGAACAAGAGCAATGGGTGCAGTGTGATGCTTGTGGGAAATGGCGACAGCTGC 1490 1500 1510 1520 1530 1540 1550 1560 1570 1580 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 AT4G32 CGGTGGATATTCTTCTTCCACCAAAGTGGTCGTGCTCTGATAATCTCTTGGATCCTGGCAGGTCTTCATGTTCCGCACCTGATGAACTCTCTCCAAGAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 CGGTGGATATTCTTCTTCCACCAAAGTGGTCGTGCTCTGATAATCTCTTGGATCCTGGCAGGTCTTCATGTTCCGCACCTGATGAACTCTCTCCAAGAGA 1590 1600 1610 1620 1630 1640 1650 1660 1670 1680 1710 1720 1730 1740 1750 1760 1770 1780 1790 1800 AT4G32 ACAGGATACACTTGTCCGGCAGAGCAAAGAGTTCAAAAGGAGGAGACTGGCATCATCAAACGAAAAGCTAAACCAGTCGCAGGATGCATCTGCTCTGAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 ACAGGATACACTTGTCCGGCAGAGCAAAGAGTTCAAAAGGAGGAGACTGGCATCATCAAACGAAAAGCTAAACCAGTCGCAGGATGCATCTGCTCTGAAT 1690 1700 1710 1720 1730 1740 1750 1760 1770 1780 1810 1820 1830 1840 1850 1860 1870 1880 1890 1900 AT4G32 AGTTTAGGAAATGCAGGCATCACCACAACCGGTGAACAGGGGGAAATCACGGTTGCAGCCACGACCAAGCATCCAAGACACCGGGCAGGGTGTTCGTGCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 AGTTTAGGAAATGCAGGCATCACCACAACCGGTGAACAGGGGGAAATCACGGTTGCAGCCACGACCAAGCATCCAAGACACCGGGCAGGGTGTTCGTGCA 1790 1800 1810 1820 1830 1840 1850 1860 1870 1880 1910 1920 1930 1940 1950 1960 1970 1980 1990 2000 AT4G32 TCGTCTGCAGCCAACCACCGAGCGGAAAAGGCAAACACAAGCCGTCATGCACTTGCACTGTGTGCGAGGCAGTGAAGAGACGATTCAGGACGCTCATGCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 TCGTCTGCAGCCAACCACCGAGCGGAAAAGGCAAACACAAGCCGTCATGCACTTGCACTGTGTGCGAGGCAGTGAAGAGACGATTCAGGACGCTCATGCT 1890 1900 1910 1920 1930 1940 1950 1960 1970 1980 2010 2020 2030 2040 2050 2060 2070 2080 2090 2100 AT4G32 GCGGAAGCGGAACAAAGGAGAGGCAGGACAGGCAAGCCAGCAGGCGCAGTCACAGTCAGAGTGCAGGGACGAGACAGAAGTGGAGAGCATTCCAGCGGTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 GCGGAAGCGGAACAAAGGAGAGGCAGGACAGGCAAGCCAGCAGGCGCAGTCACAGTCAGAGTGCAGGGACGAGACAGAAGTGGAGAGCATTCCAGCGGTT 1990 2000 2010 2020 2030 2040 2050 2060 2070 2080 2110 2120 2130 2140 2150 2160 2170 2180 2190 2200 AT4G32 GAACTAGCCGCAGGGGAAAACATCGACTTGAACTCAGACCCGGGGGCTTCCCGAGTAAGCATGATGAGGCTTCTCCAAGCTGCAGCGTTTCCTCTGGAAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 GAACTAGCCGCAGGGGAAAACATCGACTTGAACTCAGACCCGGGGGCTTCCCGAGTAAGCATGATGAGGCTTCTCCAAGCTGCAGCGTTTCCTCTGGAAG 2090 2100 2110 2120 2130 2140 2150 2160 2170 2180 2210 2220 2230 2240 2250 2260 2270 2280 2290 2300 AT4G32 CATATCTGAAACAAAAGGCTATTTCCAATACAGCAGGAGAACAGCAAAGCAGTGATATGGTCAGCACAGAACACGGTTCGTCCTCAGCCGCACAAGAAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 CATATCTGAAACAAAAGGCTATTTCCAATACAGCAGGAGAACAGCAAAGCAGTGATATGGTCAGCACAGAACACGGTTCGTCCTCAGCCGCACAAGAAAC 2190 2200 2210 2220 2230 2240 2250 2260 2270 2280 2310 2320 2330 2340 AT4G32 TGAGAAAGACACAACAAATGGAGCTCATGATCCTGTGAAC :::::::::::::::::::::::::::::::::::::::: IE_005 TGAGAAAGACACAACAAATGGAGCTCATGATCCTGTGAAC 2290 2300 2310 2320 10 20 30 40 50 60 70 80 90 100 AT4G32 MESIKVCMNALCGAASTSGEWKKGWPMRSGDLASLCDKCGCAYEQSIFCEVFHAKESGWRECNSCDKRLHCGCIASRFMMELLENGGVTCISCAKKSGLI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 MESIKVCMNALCGAASTSGEWKKGWPMRSGDLASLCDKCGCAYEQSIFCEVFHAKESGWRECNSCDKRLHCGCIASRFMMELLENGGVTCISCAKKSGLI 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G32 SMNVSHESNGKDFPSFASAEHVGSVLERTNLKHLLHFQRIDPTHSSLQMKQEESLLPSSLDALRHKTERKELSAQPNLSISLGPTLMTSPFHDAAVDDRS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 SMNVSHESNGKDFPSFASAEHVGSVLERTNLKHLLHFQRIDPTHSSLQMKQEESLLPSSLDALRHKTERKELSAQPNLSISLGPTLMTSPFHDAAVDDRS 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G32 KTNSIFQLAPRSRQLLPKPANSAPIAAGMEPSGSLVSQIHVARPPPEGRGKTQLLPRYWPRITDQELLQLSGQYPHLSNSKIIPLFEKVLSASDAGRIGR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::: IE_005 KTNSIFQLAPRSRQLLPKPANSAPIAAGMEPSGSLVSQIHVARPPPEGRGKTQLLPRYWPRITDQELLQLSGH----SNSKIIPLFEKVLSASDAGRIGR 210 220 230 240 250 260 270 280 290 310 320 330 340 350 360 370 380 390 400 AT4G32 LVLPKACAEAYFPPISLPEGLPLKIQDIKGKEWVFQFRFWPNNNSRMYVLEGVTPCIQSMQLQAGDTVTFSRTEPEGKLVMGYRKATNSTATQMFKGSSE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 LVLPKACAEAYFPPISLPEGLPLKIQDIKGKEWVFQFRFWPNNNSRMYVLEGVTPCIQSMQLQAGDTVTFSRTEPEGKLVMGYRKATNSTATQMFKGSSE 300 310 320 330 340 350 360 370 380 390 410 420 430 440 450 460 470 480 490 500 AT4G32 PNLNMFSNSLNPGCGDINWSKLEKSEDMAKDNLFLQSSLTSARKRVRNIGTKSKRLLIDSVDVLELKITWEEAQELLRPPQSTKPSIFTLENQDFEEYDE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 PNLNMFSNSLNPGCGDINWSKLEKSEDMAKDNLFLQSSLTSARKRVRNIGTKSKRLLIDSVDVLELKITWEEAQELLRPPQSTKPSIFTLENQDFEEYDE 400 410 420 430 440 450 460 470 480 490 510 520 530 540 550 560 570 580 590 600 AT4G32 PPVFGKRTLFVSRQTGEQEQWVQCDACGKWRQLPVDILLPPKWSCSDNLLDPGRSSCSAPDELSPREQDTLVRQSKEFKRRRLASSNEKLNQSQDASALN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 PPVFGKRTLFVSRQTGEQEQWVQCDACGKWRQLPVDILLPPKWSCSDNLLDPGRSSCSAPDELSPREQDTLVRQSKEFKRRRLASSNEKLNQSQDASALN 500 510 520 530 540 550 560 570 580 590 610 620 630 640 650 660 670 680 690 700 AT4G32 SLGNAGITTTGEQGEITVAATTKHPRHRAGCSCIVCSQPPSGKGKHKPSCTCTVCEAVKRRFRTLMLRKRNKGEAGQASQQAQSQSECRDETEVESIPAV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 SLGNAGITTTGEQGEITVAATTKHPRHRAGCSCIVCSQPPSGKGKHKPSCTCTVCEAVKRRFRTLMLRKRNKGEAGQASQQAQSQSECRDETEVESIPAV 600 610 620 630 640 650 660 670 680 690 710 720 730 740 750 760 770 780 AT4G32 ELAAGENIDLNSDPGASRVSMMRLLQAAAFPLEAYLKQKAISNTAGEQQSSDMVSTEHGSSSAAQETEKDTTNGAHDPVN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_005 ELAAGENIDLNSDPGASRVSMMRLLQAAAFPLEAYLKQKAISNTAGEQQSSDMVSTEHGSSSAAQETEKDTTNGAHDPVN 700 710 720 730 740 750 760 770

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