◆◆◆IE_0081 detail◆◆◆

Code nameIE_0081
LocusAT3G16350
TemplateIT_0081 (cDNA)
Donor VectorpDONR207
Forward primer8725 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGACTCGTCGGTGTTCGCATTGT)
Reverse primer8726 (GGGGACCACTTTGTACAAGAAAGCTGGGTCGATAGCCTGAATCGCGCTGTTGCCT)
Alignment with template seq.1st-2nd-
Alignment with TAIR7CDSアミノ酸配列corresponding:kushida一致-
comment
sequence 
>IE_0081_1161bp
ATGACTCGTCGGTGTTCGCATTGTAGCAACAATGGGCACAATTCACGCACGTGTCCAACGCGTGGTGGTGGCACGTGCGGTGGAAGTGGCGGAGGAGGAG
GAGGTGGTGGTGGAGGAGGGTCTGGTTCCTCCTCCGCCGTGAAGTTATTTGGTGTGAGGTTAACGGATGGCTCGATTATTAAAAAGAGTGCGAGTATGGG
TAATCTCTCGGCATTGGCTGTTGCGGCGGCGGCGGCAACGCACCACCGTTTATCTCCGTCGTCTCCTCTGGCGACGTCAAATCTTAATGATTCGCCGTTA
TCGGATCATGCCCGATACTCTAATTTGCATCATAATGAAGGGTATTTATCTGATGATCCTGCTCATGGTTCTGGGTCTAGTCACCGTCGTGGTGAGAGGA
AGAGAGGTGTTCCTTGGACTGAAGAGGAACATAGACTATTCTTAGTCGGTCTTCAGAAACTCGGGAAAGGAGATTGGCGCGGTATTTCGAGAAACTATGT
AACGTCAAGAACTCCTACACAAGTGGCTAGTCATGCTCAAAAGTATTTTATTCGACATACTAGTTCAAGCCGCAGGAAAAGACGGTCTAGCCTCTTCGAC
ATGGTTACAGATGAGATGGTAACCGATTCATCGCCAACACAGGAAGAGCAGACCTTAAACGGTTCCTCTCCAAGCAAGGAACCTGAAAAGAAAAGCTACC
TTCCTTCACTTGAGCTCTCACTCAATAATACCACAGAAGCTGAAGAGGTCGTAGCCACGGCGCCACGACAGGAAAAATCTCAAGAAGCTATAGAACCATC
AAATGGTGTTTCACCAATGCTAGTCCCGGGTGGCTTCTTTCCTCCTTGTTTTCCAGTGACTTACACGATTTGGCTCCCTGCGTCACTTCACGGAACAGAA
CATGCCTTAAACGCTGAGACTTCTTCTCAGCAGCATCAGGTCCTAAAACCAAAACCTGGATTTGCTAAAGAACGTGTGAACATGGACGAGTTGGTCGGTA
TGTCTCAGCTTAGCATAGGAATGGCGACAAGACACGAAACCGAAACTTCCCCTTCCCCGCTATCTTTGAGACTAGAGCCCTCAAGGCCATCAGCGTTTCA
CTCGAATGGCTCGGTTAATGGTGCAGATTTGAGTAAAGGCAACAGCGCGATTCAGGCTATC

>IE_0081_translated
MTRRCSHCSNNGHNSRTCPTRGGGTCGGSGGGGGGGGGGGSGSSSAVKLFGVRLTDGSIIKKSASMGNLSALAVAAAAATHHRLSPSSPLATSNLNDSPL
SDHARYSNLHHNEGYLSDDPAHGSGSSHRRGERKRGVPWTEEEHRLFLVGLQKLGKGDWRGISRNYVTSRTPTQVASHAQKYFIRHTSSSRRKRRSSLFD
MVTDEMVTDSSPTQEEQTLNGSSPSKEPEKKSYLPSLELSLNNTTEAEEVVATAPRQEKSQEAIEPSNGVSPMLVPGGFFPPCFPVTYTIWLPASLHGTE
HALNAETSSQQHQVLKPKPGFAKERVNMDELVGMSQLSIGMATRHETETSPSPLSLRLEPSRPSAFHSNGSVNGADLSKGNSAIQAI
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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