◆◆◆IE_0093 detail◆◆◆

Code name	IE_0093
Locus	AT4G08150
Template	IT_0093 (HR1900G)
Donor Vector	pDONR207
Forward primer	9047 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGGAAGAATACCAGCAT)
Reverse primer	6947 (GGGGACCACTTTGTACAAGAAAGCTGGGTCTGGACCGAGACGATAAGGTCCATC)
Alignment with template seq.	1st	-	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列corresponding:sakamoto一致	2nd	-
comment
sequence	>IE_0093_1104bp ATGGAAGAATACCAGCATGACAACAACAATAATAATAGTAGCAATTATGGTCCTGGTTACAATAATACTAACAACAACAATCATCACCACCAACACATGT TGTTTCCACATATGAGCTCTCTTCTCCCTCAAACAACCGAGAATTGCTTCCGATCTGATCATGATCAACCCAACAACAACAACAACCCATCTGTTAAATC TGAAGCTAGCTCCTCAAGAATCAATCATTACTCCATGTTAATGAGAGCCATCCACAATACTCAAGAAGCTAACAACAACAACAATGACAACGTAAGCGAT GTTGAAGCCATGAAGGCTAAAATCATTGCTCATCCTCACTACTCTACCCTCCTACAAGCTTACTTGGACTGCCAAAAGATTGGAGCTCCACCTGATGTGG TTGATAGAATTACGGCGGCACGGCAAGACTTTGAGGCTCGACAACAGCGGTCAACACCGTCTGTCTCTGCCTCCTCTAAAGACCCGGAGTTAGATCAATT CATGGAAGCATACTGTGACATGTTGGTTAAATATCGTGAGGAGCTAACAAGGCCCATTCAGGAAGCAATGGAGTTTATACGTCGTATTGAATCTCAGCTT AGCATGTTGTGTCAGAGTCCCATTCACATCCTCAACAATCCTGATGGGAAGAGTGACAATATGGGATCATCAGACGAAGAACAAGAGAATAACAGCGGAG GGGAAACAGAATTACCGGAAATAGACCCGAGGGCCGAAGATCGGGAACTCAAGAACCATTTGCTGAAGAAGTATAGTGGATACTTAAGCAGTTTGAAGCA AGAACTATCCAAGAAGAAAAAGAAAGGTAAACTTCCTAAAGAAGCACGGCAGAAGCTTCTCACGTGGTGGGAGTTGCATTACAAGTGGCCATATCCTTCT GAGTCAGAGAAGGTAGCGTTGGCGGAATCAACGGGGTTAGATCAGAAACAAATCAACAATTGGTTCATAAACCAAAGAAAGCGTCACTGGAAACCATCTG AAGACATGCAGTTCATGGTGATGGATGGTCTGCAGCACCCGCACCACGCAGCTCTGTACATGGATGGTCATTACATGGGTGATGGACCTTATCGTCTCGG TCCA >IE_0093_translated MEEYQHDNNNNNSSNYGPGYNNTNNNNHHHQHMLFPHMSSLLPQTTENCFRSDHDQPNNNNNPSVKSEASSSRINHYSMLMRAIHNTQEANNNNNDNVSD VEAMKAKIIAHPHYSTLLQAYLDCQKIGAPPDVVDRITAARQDFEARQQRSTPSVSASSKDPELDQFMEAYCDMLVKYREELTRPIQEAMEFIRRIESQL SMLCQSPIHILNNPDGKSDNMGSSDEEQENNSGGETELPEIDPRAEDRELKNHLLKKYSGYLSSLKQELSKKKKKGKLPKEARQKLLTWWELHYKWPYPS ESEKVALAESTGLDQKQINNWFINQRKRHWKPSEDMQFMVMDGLQHPHHAALYMDGHYMGDGPYRLGP
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1103/1194 (92.4%)（アミノ酸配列で367/398 (92.2%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G08 ATGGAAGAATACCAGCATGACAACAGCACCACTCCTCAAAGAGTAAGTTTCTTGTACTCTCCAATCTCTTCTTCCAACAAAAACGATAACACAAGTGATA ::::::::::::::::::: IE_009 ATGGAAGAATACCAGCATG--------------------------------------------------------------------------------- 10 110 120 130 140 150 160 170 180 190 200 AT4G08 CCAACAACAACAACAACAATAATAATAGTAGCAATTATGGTCCTGGTTACAATAATACTAACAACAACAATCATCACCACCAACACATGTTGTTTCCACA ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_009 ---------ACAACAACAATAATAATAGTAGCAATTATGGTCCTGGTTACAATAATACTAACAACAACAATCATCACCACCAACACATGTTGTTTCCACA 20 30 40 50 60 70 80 90 100 110 210 220 230 240 250 260 270 280 290 300 AT4G08 TATGAGCTCTCTTCTCCCTCAAACAACCGAGAATTGCTTCCGATCTGATCATGATCAACCCAACAACAACAACAACCCATCTGTTAAATCTGAAGCTAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_009 TATGAGCTCTCTTCTCCCTCAAACAACCGAGAATTGCTTCCGATCTGATCATGATCAACCCAACAACAACAACAACCCATCTGTTAAATCTGAAGCTAGC 120 130 140 150 160 170 180 190 200 210 310 320 330 340 350 360 370 380 390 400 AT4G08 TCCTCAAGAATCAATCATTACTCCATGTTAATGAGAGCCATCCACAATACTCAAGAAGCTAACAACAACAACAATGACAACGTAAGCGATGTTGAAGCCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_009 TCCTCAAGAATCAATCATTACTCCATGTTAATGAGAGCCATCCACAATACTCAAGAAGCTAACAACAACAACAATGACAACGTAAGCGATGTTGAAGCCA 220 230 240 250 260 270 280 290 300 310 410 420 430 440 450 460 470 480 490 500 AT4G08 TGAAGGCTAAAATCATTGCTCATCCTCACTACTCTACCCTCCTACAAGCTTACTTGGACTGCCAAAAGATTGGAGCTCCACCTGATGTGGTTGATAGAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_009 TGAAGGCTAAAATCATTGCTCATCCTCACTACTCTACCCTCCTACAAGCTTACTTGGACTGCCAAAAGATTGGAGCTCCACCTGATGTGGTTGATAGAAT 320 330 340 350 360 370 380 390 400 410 510 520 530 540 550 560 570 580 590 600 AT4G08 TACGGCGGCACGGCAAGACTTTGAGGCTCGACAACAGCGGTCAACACCGTCTGTCTCTGCCTCCTCTAGAGACCCGGAGTTAGATCAATTCATGGAAGCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::: IE_009 TACGGCGGCACGGCAAGACTTTGAGGCTCGACAACAGCGGTCAACACCGTCTGTCTCTGCCTCCTCTAAAGACCCGGAGTTAGATCAATTCATGGAAGCA 420 430 440 450 460 470 480 490 500 510 610 620 630 640 650 660 670 680 690 700 AT4G08 TACTGTGACATGTTGGTTAAATATCGTGAGGAGCTAACAAGGCCCATTCAGGAAGCAATGGAGTTTATACGTCGTATTGAATCTCAGCTTAGCATGTTGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_009 TACTGTGACATGTTGGTTAAATATCGTGAGGAGCTAACAAGGCCCATTCAGGAAGCAATGGAGTTTATACGTCGTATTGAATCTCAGCTTAGCATGTTGT 520 530 540 550 560 570 580 590 600 610 710 720 730 740 750 760 770 780 790 800 AT4G08 GTCAGAGTCCCATTCACATCCTCAACAATCCTGATGGGAAGAGTGACAATATGGGATCATCAGACGAAGAACAAGAGAATAACAGCGGAGGGGAAACAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_009 GTCAGAGTCCCATTCACATCCTCAACAATCCTGATGGGAAGAGTGACAATATGGGATCATCAGACGAAGAACAAGAGAATAACAGCGGAGGGGAAACAGA 620 630 640 650 660 670 680 690 700 710 810 820 830 840 850 860 870 880 890 900 AT4G08 ATTACCGGAAATAGACCCGAGGGCCGAAGATCGGGAACTCAAGAACCATTTGCTGAAGAAGTATAGTGGATACTTAAGCAGTTTGAAGCAAGAACTATCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_009 ATTACCGGAAATAGACCCGAGGGCCGAAGATCGGGAACTCAAGAACCATTTGCTGAAGAAGTATAGTGGATACTTAAGCAGTTTGAAGCAAGAACTATCC 720 730 740 750 760 770 780 790 800 810 910 920 930 940 950 960 970 980 990 1000 AT4G08 AAGAAGAAAAAGAAAGGTAAACTTCCTAAAGAAGCACGGCAGAAGCTTCTCACGTGGTGGGAGTTGCATTACAAGTGGCCATATCCTTCTGAGTCAGAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_009 AAGAAGAAAAAGAAAGGTAAACTTCCTAAAGAAGCACGGCAGAAGCTTCTCACGTGGTGGGAGTTGCATTACAAGTGGCCATATCCTTCTGAGTCAGAGA 820 830 840 850 860 870 880 890 900 910 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT4G08 AGGTAGCGTTGGCGGAATCAACGGGGTTAGATCAGAAACAAATCAACAATTGGTTCATAAACCAAAGAAAGCGTCACTGGAAACCATCTGAAGACATGCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_009 AGGTAGCGTTGGCGGAATCAACGGGGTTAGATCAGAAACAAATCAACAATTGGTTCATAAACCAAAGAAAGCGTCACTGGAAACCATCTGAAGACATGCA 920 930 940 950 960 970 980 990 1000 1010 1110 1120 1130 1140 1150 1160 1170 1180 1190 AT4G08 GTTCATGGTGATGGATGGTCTGCAGCACCCGCACCACGCAGCTCTGTACATGGATGGTCATTACATGGGTGATGGACCTTATCGTCTCGGTCCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_009 GTTCATGGTGATGGATGGTCTGCAGCACCCGCACCACGCAGCTCTGTACATGGATGGTCATTACATGGGTGATGGACCTTATCGTCTCGGTCCA 1020 1030 1040 1050 1060 1070 1080 1090 1100 10 20 30 40 50 60 70 80 90 100 AT4G08 MEEYQHDNSTTPQRVSFLYSPISSSNKNDNTSDTNNNNNNNNSSNYGPGYNNTNNNNHHHQHMLFPHMSSLLPQTTENCFRSDHDQPNNNNNPSVKSEAS ::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_009 MEEYQHD------------------------------NNNNNSSNYGPGYNNTNNNNHHHQHMLFPHMSSLLPQTTENCFRSDHDQPNNNNNPSVKSEAS 10 20 30 40 50 60 70 110 120 130 140 150 160 170 180 190 200 AT4G08 SSRINHYSMLMRAIHNTQEANNNNNDNVSDVEAMKAKIIAHPHYSTLLQAYLDCQKIGAPPDVVDRITAARQDFEARQQRSTPSVSASSRDPELDQFMEA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::.:::::::::: IE_009 SSRINHYSMLMRAIHNTQEANNNNNDNVSDVEAMKAKIIAHPHYSTLLQAYLDCQKIGAPPDVVDRITAARQDFEARQQRSTPSVSASSKDPELDQFMEA 80 90 100 110 120 130 140 150 160 170 210 220 230 240 250 260 270 280 290 300 AT4G08 YCDMLVKYREELTRPIQEAMEFIRRIESQLSMLCQSPIHILNNPDGKSDNMGSSDEEQENNSGGETELPEIDPRAEDRELKNHLLKKYSGYLSSLKQELS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_009 YCDMLVKYREELTRPIQEAMEFIRRIESQLSMLCQSPIHILNNPDGKSDNMGSSDEEQENNSGGETELPEIDPRAEDRELKNHLLKKYSGYLSSLKQELS 180 190 200 210 220 230 240 250 260 270 310 320 330 340 350 360 370 380 390 AT4G08 KKKKKGKLPKEARQKLLTWWELHYKWPYPSESEKVALAESTGLDQKQINNWFINQRKRHWKPSEDMQFMVMDGLQHPHHAALYMDGHYMGDGPYRLGP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_009 KKKKKGKLPKEARQKLLTWWELHYKWPYPSESEKVALAESTGLDQKQINNWFINQRKRHWKPSEDMQFMVMDGLQHPHHAALYMDGHYMGDGPYRLGP 280 290 300 310 320 330 340 350 360

このページのTOPに戻る