◆◆◆IE_0101 detail◆◆◆

Code name	IE_0101
Locus	AT4G38900
Template	IT_0101 (cDNA)
Donor Vector	pDONR207
Forward primer	9264 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGGGTGATACAGAGAAGTGTAACAGTGA)
Reverse primer	9255 (GGGGACCACTTTGTACAAGAAAGCTGGGTCTTCATTTGATTCAGATTTTGTTGCCATGGT)
Alignment with template seq.	1st	-	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列corresponding:kigoshi一致	2nd	-
comment
sequence	>IE_0101_1641bp ATGGGTGATACAGAGAAGTGTAACAGTGATATGATCCAGAGACTTCATTCATCTTTCGGCACTACTTCTTCTTCCATTCCCAAAAATCCCATTTCTCAGC TCGATTTAAACCCTAATTTCATCCGCTCATCAGCTCCTCAATTCTCCAAGCCTTTCAGTGACAGTGGCAAACGAATCGGTGTTCCTCCGTCGCACCCCAA CTTAATCCCACCGACTTCTCCGTTTTCTCAGATCCCGACCACCCGACAACCCGGTTCGCATAATTTTAACCCGGGAGGAGCTAATCATTCACGGTCAATG TCACAGCCCAACTCTTTCTTCTCTTTTGACTCCTTACCTCCGTTAAGCCCTTCTCCGTTTCGAGATCACGATGTTTCAATGGAGGATAGAGATTCCGGCG TGTTTAACAGCAACCATTCGTTGCCTCCATCGCCGTTCACGAGGTGTAATTCGACCTCTTCTAGCTCCTTGAGAGTCGGTGAGAGTTTACCTCCGAGAAA GTCTCATAGACGCTCCAACAGTGATATCCCCAGTGGGTTTAATTCGATGCCTTTGATCCCTCCGAGACCATTGGAGAGGTCTTTTTCTGGTGGGGAGTGT GCTGATTGGTCAAAGTCTAATCCTTTTGTGAAGAAGGAATCGAGCTGCGAAAGGGAAGGTGTCGGAGAGAGAGAAGCTATGGATGATCTCTTCTCAGCAT ATATGAATCTTGAAAACATTGATGTGTTGAACTCCTCTGAAGCTGATGATAGCAAGAACGGTAATGAGAATAGGGATGATATGGAGAGCAGCAGAGCAAG CGGGACCAAGACTAACGGTAGTGATACGGAAGGAGAGAGCAGCAGTGTCAATGAGAGTGCCAATAATAATATGAATTCTTCTGGTGAAAAGAGAGAGAGC GTGAAGAGAAGAGCGGCTGGAGGAGATATTGCTCCTACCACCAGACATTACAGGAGTGTTTCAGTGGACAGTTGTTTCATGGAGAAGTTGTCTTTTGGTG ATGAATCTCTAAAGCCGCCTCCTTCTCCTGGATCTATGTCAAGGAAAGTTTCCCCTACCAATTCGGTTGATGGGAACTCGGGTGCTGCTTTTAGCATCGA GTTCAATAACGGTGAGTTTACTGCAGCGGAAATGAAGAAGATCATGGCAAATGATAAACTAGCAGAGATGGCCATGTCTGACCCTAAACGTGTCAAAAGA ATCTTAGCGAACCGTCAATCCGCAGCACGGTCAAAGGAGAGGAAGATGCGGTACATAGTAGAATTGGAACACAAAGTGCAGACTCTTCAGACCGAGGCTA CCACATTGTCTGCTCAGCTCACACTTTTGCAGCGCGATATGATGGGGTTGACAAATCAGAACAATGAGCTTAAGTTCCGGCTTCAAGCAATGGAGCAACA AGCGCGTCTTCGCGATGCTCTGAACGAAGCACTGAATGGAGAAGTCCAGCGACTGAAACTGGCAATCGGTGAGAGCAGTCAGAACGAATCAGAGAGATCA AAGATGCAATCACTCAACGCTGAGATGTTCCAGCAACTCAACATCAGCCAGTTAAGACAGCAGCCACAACAGATGCAGCAACAGTCTCATCAGCAGAACC ACCAGAATGGAACCATGGCAACAAAATCTGAATCAAATGAA >IE_0101_translated MGDTEKCNSDMIQRLHSSFGTTSSSIPKNPISQLDLNPNFIRSSAPQFSKPFSDSGKRIGVPPSHPNLIPPTSPFSQIPTTRQPGSHNFNPGGANHSRSM SQPNSFFSFDSLPPLSPSPFRDHDVSMEDRDSGVFNSNHSLPPSPFTRCNSTSSSSLRVGESLPPRKSHRRSNSDIPSGFNSMPLIPPRPLERSFSGGEC ADWSKSNPFVKKESSCEREGVGEREAMDDLFSAYMNLENIDVLNSSEADDSKNGNENRDDMESSRASGTKTNGSDTEGESSSVNESANNNMNSSGEKRES VKRRAAGGDIAPTTRHYRSVSVDSCFMEKLSFGDESLKPPPSPGSMSRKVSPTNSVDGNSGAAFSIEFNNGEFTAAEMKKIMANDKLAEMAMSDPKRVKR ILANRQSAARSKERKMRYIVELEHKVQTLQTEATTLSAQLTLLQRDMMGLTNQNNELKFRLQAMEQQARLRDALNEALNGEVQRLKLAIGESSQNESERS KMQSLNAEMFQQLNISQLRQQPQQMQQQSHQQNHQNGTMATKSESNE
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1641/1659 (98.9%)（アミノ酸配列で547/553 (98.9%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G38 ATGGGTGATACAGAGAAGTGTAACAGTGATATGATCCAGAGACTTCATTCATCTTTCGGCACTACTTCTTCTTCCATTCCCAAAAATCCCATTTCTCAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 ATGGGTGATACAGAGAAGTGTAACAGTGATATGATCCAGAGACTTCATTCATCTTTCGGCACTACTTCTTCTTCCATTCCCAAAAATCCCATTTCTCAGC 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G38 TCGATTTAAACCCTAATTTCATCCGCTCATCAGCTCCTCAATTCTCCAAGCCTTTCAGTGACAGTGGCAAACGAATCGGTGTTCCTCCGTCGCACCCCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 TCGATTTAAACCCTAATTTCATCCGCTCATCAGCTCCTCAATTCTCCAAGCCTTTCAGTGACAGTGGCAAACGAATCGGTGTTCCTCCGTCGCACCCCAA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G38 CTTAATCCCACCGACTTCTCCGTTTTCTCAGATCCCGACCACCCGACAACCCGGTTCGCATAATTTTAACCCGGGAGGAGCTAATCATTCACGGTCAATG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 CTTAATCCCACCGACTTCTCCGTTTTCTCAGATCCCGACCACCCGACAACCCGGTTCGCATAATTTTAACCCGGGAGGAGCTAATCATTCACGGTCAATG 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT4G38 TCACAGCCCAACTCTTTCTTCTCTTTTGACTCCTTACCTCCGTTAAGCCCTTCTCCGTTTCGAGATCACGATGTTTCAATGGAGGATAGAGATTCCGGCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 TCACAGCCCAACTCTTTCTTCTCTTTTGACTCCTTACCTCCGTTAAGCCCTTCTCCGTTTCGAGATCACGATGTTTCAATGGAGGATAGAGATTCCGGCG 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT4G38 TGTTTAACAGCAACCATTCGTTGCCTCCATCGCCGTTCACGAGGTGTAATTCGACCTCTTCTAGCTCCTTGAGAGTCGGTGAGAGTTTACCTCCGAGAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 TGTTTAACAGCAACCATTCGTTGCCTCCATCGCCGTTCACGAGGTGTAATTCGACCTCTTCTAGCTCCTTGAGAGTCGGTGAGAGTTTACCTCCGAGAAA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT4G38 GTCTCATAGACGCTCCAACAGTGATATCCCCAGTGGGTTTAATTCGATGCCTTTGATCCCTCCGAGACCATTGGAGAGGTCTTTTTCTGGTGGGGAGTGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 GTCTCATAGACGCTCCAACAGTGATATCCCCAGTGGGTTTAATTCGATGCCTTTGATCCCTCCGAGACCATTGGAGAGGTCTTTTTCTGGTGGGGAGTGT 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT4G38 GCTGATTGGTCAAAGTCTAATCCTTTTGTGAAGAAGGAATCGAGCTGCGAAAGGGAAGGTGTCGGAGAGAGAGAAGCTATGGATGATCTCTTCTCAGCAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 GCTGATTGGTCAAAGTCTAATCCTTTTGTGAAGAAGGAATCGAGCTGCGAAAGGGAAGGTGTCGGAGAGAGAGAAGCTATGGATGATCTCTTCTCAGCAT 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT4G38 ATATGAATCTTGAAAACATTGATGTGTTGAACTCCTCTGAAGCTGATGATAGCAAGAACGGTAATGAGAATAGGGATGATATGGAGAGCAGCAGAGCAAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 ATATGAATCTTGAAAACATTGATGTGTTGAACTCCTCTGAAGCTGATGATAGCAAGAACGGTAATGAGAATAGGGATGATATGGAGAGCAGCAGAGCAAG 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT4G38 CGGGACCAAGACTAACGGTAGTGATACGGAAGGAGAGAGCAGCAGTGTCAATGAGAGTGCCAATAATAATATGAATTCTTCTGGTGAAAAGAGAGAGAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 CGGGACCAAGACTAACGGTAGTGATACGGAAGGAGAGAGCAGCAGTGTCAATGAGAGTGCCAATAATAATATGAATTCTTCTGGTGAAAAGAGAGAGAGC 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT4G38 GTGAAGAGAAGAGCGGCTGGAGGAGATATTGCTCCTACCACCAGACATTACAGGAGTGTTTCAGTGGACAGTTGTTTCATGGAGAAGTTGTCTTTTGGTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 GTGAAGAGAAGAGCGGCTGGAGGAGATATTGCTCCTACCACCAGACATTACAGGAGTGTTTCAGTGGACAGTTGTTTCATGGAGAAGTTGTCTTTTGGTG 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT4G38 ATGAATCTCTAAAGCCGCCTCCTTCTCCTGGATCTATGTCAAGGAAAGTTTCCCCTACCAATTCGGTTGATGGGAACTCGGGTGCTGCTTTTAGCATCGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 ATGAATCTCTAAAGCCGCCTCCTTCTCCTGGATCTATGTCAAGGAAAGTTTCCCCTACCAATTCGGTTGATGGGAACTCGGGTGCTGCTTTTAGCATCGA 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT4G38 GTTCAATAACGGTGAGTTTACTGCAGCGGAAATGAAGAAGATCATGGCAAATGATAAACTAGCAGAGATGGCCATGTCTGACCCTAAACGTGTCAAAAGG ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 GTTCAATAACGGTGAGTTTACTGCAGCGGAAATGAAGAAGATCATGGCAAATGATAAACTAGCAGAGATGGCCATGTCTGACCCTAAACGTGTCAAA--- 1110 1120 1130 1140 1150 1160 1170 1180 1190 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT4G38 AATGATCCTCTTTTCAGAATCTTAGCGAACCGTCAATCCGCAGCACGGTCAAAGGAGAGGAAGATGCGGTACATAGTAGAATTGGAACACAAAGTGCAGA ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 ---------------AGAATCTTAGCGAACCGTCAATCCGCAGCACGGTCAAAGGAGAGGAAGATGCGGTACATAGTAGAATTGGAACACAAAGTGCAGA 1200 1210 1220 1230 1240 1250 1260 1270 1280 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT4G38 CTCTTCAGACCGAGGCTACCACATTGTCTGCTCAGCTCACACTTTTGCAGCGCGATATGATGGGGTTGACAAATCAGAACAATGAGCTTAAGTTCCGGCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 CTCTTCAGACCGAGGCTACCACATTGTCTGCTCAGCTCACACTTTTGCAGCGCGATATGATGGGGTTGACAAATCAGAACAATGAGCTTAAGTTCCGGCT 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT4G38 TCAAGCAATGGAGCAACAAGCGCGTCTTCGCGATGCTCTGAACGAAGCACTGAATGGAGAAGTCCAGCGACTGAAACTGGCAATCGGTGAGAGCAGTCAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 TCAAGCAATGGAGCAACAAGCGCGTCTTCGCGATGCTCTGAACGAAGCACTGAATGGAGAAGTCCAGCGACTGAAACTGGCAATCGGTGAGAGCAGTCAG 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 AT4G38 AACGAATCAGAGAGATCAAAGATGCAATCACTCAACGCTGAGATGTTCCAGCAACTCAACATCAGCCAGTTAAGACAGCAGCCACAACAGATGCAGCAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 AACGAATCAGAGAGATCAAAGATGCAATCACTCAACGCTGAGATGTTCCAGCAACTCAACATCAGCCAGTTAAGACAGCAGCCACAACAGATGCAGCAAC 1490 1500 1510 1520 1530 1540 1550 1560 1570 1580 1610 1620 1630 1640 1650 AT4G38 AGTCTCATCAGCAGAACCACCAGAATGGAACCATGGCAACAAAATCTGAATCAAATGAA ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 AGTCTCATCAGCAGAACCACCAGAATGGAACCATGGCAACAAAATCTGAATCAAATGAA 1590 1600 1610 1620 1630 1640 10 20 30 40 50 60 70 80 90 100 AT4G38 MGDTEKCNSDMIQRLHSSFGTTSSSIPKNPISQLDLNPNFIRSSAPQFSKPFSDSGKRIGVPPSHPNLIPPTSPFSQIPTTRQPGSHNFNPGGANHSRSM :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 MGDTEKCNSDMIQRLHSSFGTTSSSIPKNPISQLDLNPNFIRSSAPQFSKPFSDSGKRIGVPPSHPNLIPPTSPFSQIPTTRQPGSHNFNPGGANHSRSM 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G38 SQPNSFFSFDSLPPLSPSPFRDHDVSMEDRDSGVFNSNHSLPPSPFTRCNSTSSSSLRVGESLPPRKSHRRSNSDIPSGFNSMPLIPPRPLERSFSGGEC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 SQPNSFFSFDSLPPLSPSPFRDHDVSMEDRDSGVFNSNHSLPPSPFTRCNSTSSSSLRVGESLPPRKSHRRSNSDIPSGFNSMPLIPPRPLERSFSGGEC 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G38 ADWSKSNPFVKKESSCEREGVGEREAMDDLFSAYMNLENIDVLNSSEADDSKNGNENRDDMESSRASGTKTNGSDTEGESSSVNESANNNMNSSGEKRES :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 ADWSKSNPFVKKESSCEREGVGEREAMDDLFSAYMNLENIDVLNSSEADDSKNGNENRDDMESSRASGTKTNGSDTEGESSSVNESANNNMNSSGEKRES 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT4G38 VKRRAAGGDIAPTTRHYRSVSVDSCFMEKLSFGDESLKPPPSPGSMSRKVSPTNSVDGNSGAAFSIEFNNGEFTAAEMKKIMANDKLAEMAMSDPKRVKR ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 VKRRAAGGDIAPTTRHYRSVSVDSCFMEKLSFGDESLKPPPSPGSMSRKVSPTNSVDGNSGAAFSIEFNNGEFTAAEMKKIMANDKLAEMAMSDPKRVK- 310 320 330 340 350 360 370 380 390 410 420 430 440 450 460 470 480 490 500 AT4G38 NDPLFRILANRQSAARSKERKMRYIVELEHKVQTLQTEATTLSAQLTLLQRDMMGLTNQNNELKFRLQAMEQQARLRDALNEALNGEVQRLKLAIGESSQ ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 -----RILANRQSAARSKERKMRYIVELEHKVQTLQTEATTLSAQLTLLQRDMMGLTNQNNELKFRLQAMEQQARLRDALNEALNGEVQRLKLAIGESSQ 400 410 420 430 440 450 460 470 480 490 510 520 530 540 550 AT4G38 NESERSKMQSLNAEMFQQLNISQLRQQPQQMQQQSHQQNHQNGTMATKSESNE ::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_010 NESERSKMQSLNAEMFQQLNISQLRQQPQQMQQQSHQQNHQNGTMATKSESNE 500 510 520 530 540

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