| Code name | IE_0103 | |||
|---|---|---|---|---|
| Locus | AT5G06250 | |||
| Template | IT_0103 (cDNA) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | 8414 (GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGTCAGTCAACCATTACTCCACA) | |||
| Reverse primer | 8415 (GGGGACCACTTTGTACAAGAAAGCTGGGTCTAAAAGAGTTAAAATTACCATGTTA) | |||
| Alignment with template seq. | 1st | - | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列corresponding:kushida一致 | - | ||
| comment | ||||
| sequence |
>IE_0103_801bp ATGTCAGTCAACCATTACTCCACAGACCACCACCACACTCTCTTGTGGCAGCAACAGCAACACCGCCACACCACCGACACATCGGAGACAACCACCACCG CCACATGGCTCCACGACGACCTAAAAGAGTCACTCTTCGAGAAGTCTCTCACACCAAGCGACGTCGGGAAACTCAACCGCCTCGTCATACCAAAACAACA CGCAGAGAAATACTTCCCTCTCAATGCCGTCCTAGTCTCCTCTGCTGCTGCTGACACGTCATCTTCGGAGAAAGGGATGCTTCTAAGCTTTGAAGACGAG TCAGGCAAGTCATGGAGGTTCAGATACTCTTACTGGAACAGCAGTCAAAGCTATGTCTTGACTAAAGGATGGAGCAGATTAGTCAAAGACAAACAGCTCG ATCCAGGCGACGTTGTTTTCTTCCAACGACACCGTTCTGATTCTAGGAGACTCTTCATTGGCTGGCGCAGACGTGGACAAGGCTCCTCATCCTCCGTCGC GGCCACTAACTCCGCCGTGAATACGAGTTCTATGGGAGCTCTTTCTTATCATCAAATCCACGCCACTAGAGCCGCCGTAGCAACAGCGGCTGAGACTCAC AGCACACCGTCGTCTTCCGTCGTCGGGAGCTCAAGGACGGTGAGGCTTTTCGGTGTGAATCTGGAGTGTCAAATGGATGAAAACGACGGAGATGATTCTG TTGCAGTTGCCACCACCGTTGAATCTCCCGACGGTTACTACGGCCAAAACATGTACTATTATTACTCTCATCCTCATAACATGGTAATTTTAACTCTTTT A >IE_0103_translated MSVNHYSTDHHHTLLWQQQQHRHTTDTSETTTTATWLHDDLKESLFEKSLTPSDVGKLNRLVIPKQHAEKYFPLNAVLVSSAAADTSSSEKGMLLSFEDE SGKSWRFRYSYWNSSQSYVLTKGWSRLVKDKQLDPGDVVFFQRHRSDSRRLFIGWRRRGQGSSSSVAATNSAVNTSSMGALSYHQIHATRAAVATAAETH STPSSSVVGSSRTVRLFGVNLECQMDENDGDDSVAVATTVESPDGYYGQNMYYYYSHPHNMVILTLL |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 800/801 (99.9%)（アミノ酸配列で266/267 (99.6%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT5G06 ATGTCAGTCAACCATTACTCCACAGACCACCACCACACTCTCTTGTGGCAGCAACAGCAACACCGCCACACCACCGACACATCGGAGACAACCACCACCG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_010 ATGTCAGTCAACCATTACTCCACAGACCACCACCACACTCTCTTGTGGCAGCAACAGCAACACCGCCACACCACCGACACATCGGAGACAACCACCACCG
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT5G06 CCACATGGCTCCACGACGACCTAAAAGAGTCACTCTTCGAGAAGTCTCTCACACCAAGCGACGTCGGGAAACTCAACCGCCTCGTCATACCAAAACAACA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_010 CCACATGGCTCCACGACGACCTAAAAGAGTCACTCTTCGAGAAGTCTCTCACACCAAGCGACGTCGGGAAACTCAACCGCCTCGTCATACCAAAACAACA
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270 280 290 300
AT5G06 CGCAGAGAAATACTTCCCTCTCAATGCCGTCCTAGTCTCCTCTGCTGCTGCTGACACGTCATCTTCGGAGAAAGGGATGCTTCTAAGCTTTGAAGACGAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_010 CGCAGAGAAATACTTCCCTCTCAATGCCGTCCTAGTCTCCTCTGCTGCTGCTGACACGTCATCTTCGGAGAAAGGGATGCTTCTAAGCTTTGAAGACGAG
210 220 230 240 250 260 270 280 290 300
310 320 330 340 350 360 370 380 390 400
AT5G06 TCAGGCAAGTCATGGAGGTTCAGATACTCTTACTGGAACAGCAGTCAAAGCTATGTCTTGACTAAAGGATGGAGCAGATTTGTCAAAGACAAACAGCTCG
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::
IE_010 TCAGGCAAGTCATGGAGGTTCAGATACTCTTACTGGAACAGCAGTCAAAGCTATGTCTTGACTAAAGGATGGAGCAGATTAGTCAAAGACAAACAGCTCG
310 320 330 340 350 360 370 380 390 400
410 420 430 440 450 460 470 480 490 500
AT5G06 ATCCAGGCGACGTTGTTTTCTTCCAACGACACCGTTCTGATTCTAGGAGACTCTTCATTGGCTGGCGCAGACGTGGACAAGGCTCCTCATCCTCCGTCGC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_010 ATCCAGGCGACGTTGTTTTCTTCCAACGACACCGTTCTGATTCTAGGAGACTCTTCATTGGCTGGCGCAGACGTGGACAAGGCTCCTCATCCTCCGTCGC
410 420 430 440 450 460 470 480 490 500
510 520 530 540 550 560 570 580 590 600
AT5G06 GGCCACTAACTCCGCCGTGAATACGAGTTCTATGGGAGCTCTTTCTTATCATCAAATCCACGCCACTAGAGCCGCCGTAGCAACAGCGGCTGAGACTCAC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_010 GGCCACTAACTCCGCCGTGAATACGAGTTCTATGGGAGCTCTTTCTTATCATCAAATCCACGCCACTAGAGCCGCCGTAGCAACAGCGGCTGAGACTCAC
510 520 530 540 550 560 570 580 590 600
610 620 630 640 650 660 670 680 690 700
AT5G06 AGCACACCGTCGTCTTCCGTCGTCGGGAGCTCAAGGACGGTGAGGCTTTTCGGTGTGAATCTGGAGTGTCAAATGGATGAAAACGACGGAGATGATTCTG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_010 AGCACACCGTCGTCTTCCGTCGTCGGGAGCTCAAGGACGGTGAGGCTTTTCGGTGTGAATCTGGAGTGTCAAATGGATGAAAACGACGGAGATGATTCTG
610 620 630 640 650 660 670 680 690 700
710 720 730 740 750 760 770 780 790 800
AT5G06 TTGCAGTTGCCACCACCGTTGAATCTCCCGACGGTTACTACGGCCAAAACATGTACTATTATTACTCTCATCCTCATAACATGGTAATTTTAACTCTTTT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_010 TTGCAGTTGCCACCACCGTTGAATCTCCCGACGGTTACTACGGCCAAAACATGTACTATTATTACTCTCATCCTCATAACATGGTAATTTTAACTCTTTT
710 720 730 740 750 760 770 780 790 800
AT5G06 A
:
IE_010 A
10 20 30 40 50 60 70 80 90 100
AT5G06 MSVNHYSTDHHHTLLWQQQQHRHTTDTSETTTTATWLHDDLKESLFEKSLTPSDVGKLNRLVIPKQHAEKYFPLNAVLVSSAAADTSSSEKGMLLSFEDE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_010 MSVNHYSTDHHHTLLWQQQQHRHTTDTSETTTTATWLHDDLKESLFEKSLTPSDVGKLNRLVIPKQHAEKYFPLNAVLVSSAAADTSSSEKGMLLSFEDE
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT5G06 SGKSWRFRYSYWNSSQSYVLTKGWSRFVKDKQLDPGDVVFFQRHRSDSRRLFIGWRRRGQGSSSSVAATNSAVNTSSMGALSYHQIHATRAAVATAAETH
:::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_010 SGKSWRFRYSYWNSSQSYVLTKGWSRLVKDKQLDPGDVVFFQRHRSDSRRLFIGWRRRGQGSSSSVAATNSAVNTSSMGALSYHQIHATRAAVATAAETH
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260
AT5G06 STPSSSVVGSSRTVRLFGVNLECQMDENDGDDSVAVATTVESPDGYYGQNMYYYYSHPHNMVILTLL
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_010 STPSSSVVGSSRTVRLFGVNLECQMDENDGDDSVAVATTVESPDGYYGQNMYYYYSHPHNMVILTLL
210 220 230 240 250 260
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