| Code name | IE_01A03 | |||
|---|---|---|---|---|
| Locus | AT1G10585 | |||
| Template | IT_01A03 (HR0478) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_01A03 (ggggacaagtttgtacaaaaaagcaggcttcATGATCCAATTGAAAGAGAATGTAAATT) | |||
| Reverse primer | IPR_01A03 (ggggaccactttgtacaagaaagctgggttTGCTTCAAAATATATATATCCATAGATGAT) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列67.40%一致 | - | ||
| comment | 1st:Templateの配列とは一致 | |||
| sequence |
>IE_01A03_366bp ATGATCCAATTGAAAGAGAATGTAAATTATTTGAAAGAGAAGAAAAGGACATTGTTACAAGGAGAACTCGGGAATCTCTACGAAGGGTCGTTTCTTCTAC CCAAACTCAGTATTCGTTCGCGGGATTCGACCATAGAAATGAATCTGATCATGGATCTAAACATGAAAAGAGTAATGTTACACGAGCTTGTGAGTATTTT TGAAGAAGAAGGAGCTCAAGTAATGAGTGCTAATCTTCAGAACTTGAATGATAGGACCACTTACACAATCATAGCCCAGGCTATCATTAGTCGGATTGGC ATTGATCCATCAAGGATAGAAGAGAGAGTACGGAAAATCATCTATGGATATATATATTTTGAAGCA >IE_01A03_translated MIQLKENVNYLKEKKRTLLQGELGNLYEGSFLLPKLSIRSRDSTIEMNLIMDLNMKRVMLHELVSIFEEEGAQVMSANLQNLNDRTTYTIIAQAIISRIG IDPSRIEERVRKIIYGYIYFEA |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 366/543 (67.4%)（アミノ酸配列で122/181 (67.4%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT1G10 ATGGGGAGAGCAAGAGAAATAGGAGAAGGAAACTCATCGTCGTTAAGGGAACAACGAAACCTCAGAGAGAAGGATCGAAGGATGCGCATGAAACATCTCT
IE_01A ----------------------------------------------------------------------------------------------------
110 120 130 140 150 160 170 180 190 200
AT1G10 TCTCTATACTTTCTTCTCATGTTTCTCCCACTCGCAAGTTACCAGTGCCTCACCTTATAGATCAAGCGACATCATACATGATCCAATTGAAAGAGAATGT
:::::::::::::::::::::::
IE_01A -----------------------------------------------------------------------------ATGATCCAATTGAAAGAGAATGT
10 20
210 220 230 240 250 260 270 280 290 300
AT1G10 AAATTATTTGAAAGAGAAGAAAAGGACATTGTTACAAGGAGAACTCGGGAATCTCTACGAAGGGTCGTTTCTTCTACCCAAACTCAGTATTCGTTCGCGG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01A AAATTATTTGAAAGAGAAGAAAAGGACATTGTTACAAGGAGAACTCGGGAATCTCTACGAAGGGTCGTTTCTTCTACCCAAACTCAGTATTCGTTCGCGG
30 40 50 60 70 80 90 100 110 120
310 320 330 340 350 360 370 380 390 400
AT1G10 GATTCGACCATAGAAATGAATCTGATCATGGATCTAAACATGAAAAGAGTAATGTTACACGAGCTTGTGAGTATTTTTGAAGAAGAAGGAGCTCAAGTAA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01A GATTCGACCATAGAAATGAATCTGATCATGGATCTAAACATGAAAAGAGTAATGTTACACGAGCTTGTGAGTATTTTTGAAGAAGAAGGAGCTCAAGTAA
130 140 150 160 170 180 190 200 210 220
410 420 430 440 450 460 470 480 490 500
AT1G10 TGAGTGCTAATCTTCAGAACTTGAATGATAGGACCACTTACACAATCATAGCCCAGGCTATCATTAGTCGGATTGGCATTGATCCATCAAGGATAGAAGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01A TGAGTGCTAATCTTCAGAACTTGAATGATAGGACCACTTACACAATCATAGCCCAGGCTATCATTAGTCGGATTGGCATTGATCCATCAAGGATAGAAGA
230 240 250 260 270 280 290 300 310 320
510 520 530 540
AT1G10 GAGAGTACGGAAAATCATCTATGGATATATATATTTTGAAGCA
:::::::::::::::::::::::::::::::::::::::::::
IE_01A GAGAGTACGGAAAATCATCTATGGATATATATATTTTGAAGCA
330 340 350 360
10 20 30 40 50 60 70 80 90 100
AT1G10 MGRAREIGEGNSSSLREQRNLREKDRRMRMKHLFSILSSHVSPTRKLPVPHLIDQATSYMIQLKENVNYLKEKKRTLLQGELGNLYEGSFLLPKLSIRSR
:::::::::::::::::::::::::::::::::::::::::
IE_01A -----------------------------------------------------------MIQLKENVNYLKEKKRTLLQGELGNLYEGSFLLPKLSIRSR
10 20 30 40
110 120 130 140 150 160 170 180
AT1G10 DSTIEMNLIMDLNMKRVMLHELVSIFEEEGAQVMSANLQNLNDRTTYTIIAQAIISRIGIDPSRIEERVRKIIYGYIYFEA
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01A DSTIEMNLIMDLNMKRVMLHELVSIFEEEGAQVMSANLQNLNDRTTYTIIAQAIISRIGIDPSRIEERVRKIIYGYIYFEA
50 60 70 80 90 100 110 120
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