| Code name | IE_01B02 | |||
|---|---|---|---|---|
| Locus | AT1G59530 | |||
| Template | IT_01B02 (HR1432) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_01B02 (ggggacaagtttgtacaaaaaagcaggcttcATGTTTTATACTAATATGGAGACGGTTC) | |||
| Reverse primer | IPR_01B02 (ggggaccactttgtacaagaaagctgggttAATCTCGAGCGTTGTGATGTAATTTACACT) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列98.60%一致 | - | ||
| comment | 1st:Templateの配列とは一致 | |||
| sequence |
>IE_01B02_444bp ATGTTTTATACTAATATGGAGACGGTTCATAACTGCTTCAATGATGCTACGATAACCGGTGATGAAATCATTGATATATTGGCGTTTCTTCAATCAGACG AGTCGGACAATCCAAGCGGTATAAACGAGGTAGTACCGGTGGATGATAAGAAGAGAAGACGGACGATGTCAAACCGGGAATCGGCCAAGAGATCGAGGAT GAAAAAGAAGAAACGTTTTGAAGAGTTAACTGAAGAGGTGAACCGGTTAAACATAAGAAATCAGGAGCTGAAAAACCGGTTAGCTAATGTTGTTAGTTGT GGTAATTTTATGTCTAGGGAGAATAACCGGTTGAAAACCGAATCGGTTTGCCTTGAGATAAGGCTTTTAGAGTTGTACCGTTTTTTAGTGGCCATGCAAT CGCCAATCTCAACGAGTGTAAATTACATCACAACGCTCGAGATT >IE_01B02_translated MFYTNMETVHNCFNDATITGDEIIDILAFLQSDESDNPSGINEVVPVDDKKRRRTMSNRESAKRSRMKKKKRFEELTEEVNRLNIRNQELKNRLANVVSC GNFMSRENNRLKTESVCLEIRLLELYRFLVAMQSPISTSVNYITTLEI |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 442/444 (99.5%)（アミノ酸配列で146/148 (98.6%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT1G59 ATGTTTTATACTAATATGGAGACGGTTCATAACTGCTTCAATGATGCTACGATAACCGGTGATGAAATCATTGATATATTGGCGTTTCTTCAATCAGACG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01B ATGTTTTATACTAATATGGAGACGGTTCATAACTGCTTCAATGATGCTACGATAACCGGTGATGAAATCATTGATATATTGGCGTTTCTTCAATCAGACG
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT1G59 AGTCGGACAATCCAAGCGGTATAAACGAGGTAGTACCGGTGGATGATAAGAAGAGAAGACGGACGATATCAAACCGGGAATCGGCCAAGAGATCGAGGAT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::
IE_01B AGTCGGACAATCCAAGCGGTATAAACGAGGTAGTACCGGTGGATGATAAGAAGAGAAGACGGACGATGTCAAACCGGGAATCGGCCAAGAGATCGAGGAT
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270 280 290 300
AT1G59 GAAAAAGAAGAAACGTTTTGAAGAGTTAACTGAAGAGGTGAACCGGTTAAACATAAGAAATCAGGAGCTGAAAAACCGGTTAGCTAATGTTGTTAGTTGT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01B GAAAAAGAAGAAACGTTTTGAAGAGTTAACTGAAGAGGTGAACCGGTTAAACATAAGAAATCAGGAGCTGAAAAACCGGTTAGCTAATGTTGTTAGTTGT
210 220 230 240 250 260 270 280 290 300
310 320 330 340 350 360 370 380 390 400
AT1G59 GGTAATTTTATATCTAGGGAGAATAACCGGTTGAAAACCGAATCGGTTTGCCTTGAGATAAGGCTTTTAGAGTTGTACCGTTTTTTAGTGGCCATGCAAT
::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01B GGTAATTTTATGTCTAGGGAGAATAACCGGTTGAAAACCGAATCGGTTTGCCTTGAGATAAGGCTTTTAGAGTTGTACCGTTTTTTAGTGGCCATGCAAT
310 320 330 340 350 360 370 380 390 400
410 420 430 440
AT1G59 CGCCAATCTCAACGAGTGTAAATTACATCACAACGCTCGAGATT
::::::::::::::::::::::::::::::::::::::::::::
IE_01B CGCCAATCTCAACGAGTGTAAATTACATCACAACGCTCGAGATT
410 420 430 440
10 20 30 40 50 60 70 80 90 100
AT1G59 MFYTNMETVHNCFNDATITGDEIIDILAFLQSDESDNPSGINEVVPVDDKKRRRTISNRESAKRSRMKKKKRFEELTEEVNRLNIRNQELKNRLANVVSC
:::::::::::::::::::::::::::::::::::::::::::::::::::::::.::::::::::::::::::::::::::::::::::::::::::::
IE_01B MFYTNMETVHNCFNDATITGDEIIDILAFLQSDESDNPSGINEVVPVDDKKRRRTMSNRESAKRSRMKKKKRFEELTEEVNRLNIRNQELKNRLANVVSC
10 20 30 40 50 60 70 80 90 100
110 120 130 140
AT1G59 GNFISRENNRLKTESVCLEIRLLELYRFLVAMQSPISTSVNYITTLEI
:::.::::::::::::::::::::::::::::::::::::::::::::
IE_01B GNFMSRENNRLKTESVCLEIRLLELYRFLVAMQSPISTSVNYITTLEI
110 120 130 140
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