| Code name | IE_01C12 | |||
|---|---|---|---|---|
| Locus | AT5G52600 | |||
| Template | IT_01C12 (HR2199) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_01C12 (ggggacaagtttgtacaaaaaagcaggcttcATGGAATGCAAAAGAGAAGAAGGGAAGT) | |||
| Reverse primer | IPR_01C12 (ggggaccactttgtacaagaaagctgggttAAGCAGAGGAAAGAAATCGGTAAAGGAAAA) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列99.50%一致 | - | ||
| comment | 1st:Templateの配列とは一致 | |||
| sequence |
>IE_01C12_603bp ATGGAATGCAAAAGAGAAGAAGGGAAGTCTTACGTGAAGAGAGGGTTGTGGAAACCAGAAGAAGATATGATATTAAAAAGCTATGTTGAGACTCATGGTG AAGGAAACTGGGCAGACATTTCTCGTAGATCCGGGTTGAAGAGAGGAGGAAAAAGCTGTAGGCAGAGATGGAAGAACTATCTAAGACCAAATATCAAAAG AGGAAGCATGTCACCACAAGAACAAGACCTTATCATCCGCATGCATAAGCTTCTTGGAAACAGATGGTCGTTGATCGCTGGTCGCCTTCCAGGTCGTACT GACAATGAAGTGAAGAACTACTGGAATACTCATTTGAACAAGAAACCTAATTCCCGAAGACAGAATGCACCTGAATCAATCGTCGGCGCCACTCCTTTCA CGGATAAGCCAGTTATGTCTACAGAACTGAGAAGAAGCCATGGAGAAGGAGGAGAAGAGGAGAGCAATACCTGGATGGAGGAGACCAACCACTTTGGCTA TGACGTCCACGTAGGATCTCCCTTGCCACTTATTTCCCACTACCCAGACAACACTCTCGTGTTTGACCCATGTTTTTCCTTTACCGATTTCTTTCCTCTG CTT >IE_01C12_translated MECKREEGKSYVKRGLWKPEEDMILKSYVETHGEGNWADISRRSGLKRGGKSCRQRWKNYLRPNIKRGSMSPQEQDLIIRMHKLLGNRWSLIAGRLPGRT DNEVKNYWNTHLNKKPNSRRQNAPESIVGATPFTDKPVMSTELRRSHGEGGEEESNTWMEETNHFGYDVHVGSPLPLISHYPDNTLVFDPCFSFTDFFPL L |
|||
| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 602/603 (99.8%)（アミノ酸配列で200/201 (99.5%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT5G52 ATGGAATGCAAAAGAGAAGAAGGGAAGTCTTACGTGAAGAGAGGGTTGTGGAAACCAGAAGAAGATATGATATTAAAAAGCTATGTTGAGACTCATGGTG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01C ATGGAATGCAAAAGAGAAGAAGGGAAGTCTTACGTGAAGAGAGGGTTGTGGAAACCAGAAGAAGATATGATATTAAAAAGCTATGTTGAGACTCATGGTG
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT5G52 AAGGAAACTGGGCAGACATTTCTCGTAGATCCGGGTTGAAGAGAGGAGGAAAAAGCTGTAGGCTGAGATGGAAGAACTATCTAAGACCAAATATCAAAAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::
IE_01C AAGGAAACTGGGCAGACATTTCTCGTAGATCCGGGTTGAAGAGAGGAGGAAAAAGCTGTAGGCAGAGATGGAAGAACTATCTAAGACCAAATATCAAAAG
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270 280 290 300
AT5G52 AGGAAGCATGTCACCACAAGAACAAGACCTTATCATCCGCATGCATAAGCTTCTTGGAAACAGATGGTCGTTGATCGCTGGTCGCCTTCCAGGTCGTACT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01C AGGAAGCATGTCACCACAAGAACAAGACCTTATCATCCGCATGCATAAGCTTCTTGGAAACAGATGGTCGTTGATCGCTGGTCGCCTTCCAGGTCGTACT
210 220 230 240 250 260 270 280 290 300
310 320 330 340 350 360 370 380 390 400
AT5G52 GACAATGAAGTGAAGAACTACTGGAATACTCATTTGAACAAGAAACCTAATTCCCGAAGACAGAATGCACCTGAATCAATCGTCGGCGCCACTCCTTTCA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01C GACAATGAAGTGAAGAACTACTGGAATACTCATTTGAACAAGAAACCTAATTCCCGAAGACAGAATGCACCTGAATCAATCGTCGGCGCCACTCCTTTCA
310 320 330 340 350 360 370 380 390 400
410 420 430 440 450 460 470 480 490 500
AT5G52 CGGATAAGCCAGTTATGTCTACAGAACTGAGAAGAAGCCATGGAGAAGGAGGAGAAGAGGAGAGCAATACCTGGATGGAGGAGACCAACCACTTTGGCTA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01C CGGATAAGCCAGTTATGTCTACAGAACTGAGAAGAAGCCATGGAGAAGGAGGAGAAGAGGAGAGCAATACCTGGATGGAGGAGACCAACCACTTTGGCTA
410 420 430 440 450 460 470 480 490 500
510 520 530 540 550 560 570 580 590 600
AT5G52 TGACGTCCACGTAGGATCTCCCTTGCCACTTATTTCCCACTACCCAGACAACACTCTCGTGTTTGACCCATGTTTTTCCTTTACCGATTTCTTTCCTCTG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01C TGACGTCCACGTAGGATCTCCCTTGCCACTTATTTCCCACTACCCAGACAACACTCTCGTGTTTGACCCATGTTTTTCCTTTACCGATTTCTTTCCTCTG
510 520 530 540 550 560 570 580 590 600
AT5G52 CTT
:::
IE_01C CTT
10 20 30 40 50 60 70 80 90 100
AT5G52 MECKREEGKSYVKRGLWKPEEDMILKSYVETHGEGNWADISRRSGLKRGGKSCRLRWKNYLRPNIKRGSMSPQEQDLIIRMHKLLGNRWSLIAGRLPGRT
:::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::
IE_01C MECKREEGKSYVKRGLWKPEEDMILKSYVETHGEGNWADISRRSGLKRGGKSCRQRWKNYLRPNIKRGSMSPQEQDLIIRMHKLLGNRWSLIAGRLPGRT
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT5G52 DNEVKNYWNTHLNKKPNSRRQNAPESIVGATPFTDKPVMSTELRRSHGEGGEEESNTWMEETNHFGYDVHVGSPLPLISHYPDNTLVFDPCFSFTDFFPL
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01C DNEVKNYWNTHLNKKPNSRRQNAPESIVGATPFTDKPVMSTELRRSHGEGGEEESNTWMEETNHFGYDVHVGSPLPLISHYPDNTLVFDPCFSFTDFFPL
110 120 130 140 150 160 170 180 190 200
AT5G52 L
:
IE_01C L
|
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