| Code name | IE_01D05 | |||
|---|---|---|---|---|
| Locus | AT1G12540 | |||
| Template | IT_01D05 (HR2362) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_01D05 (ggggacaagtttgtacaaaaaagcaggcttcATGACATTAAGCGAAATCATGAAAGGAG) | |||
| Reverse primer | IPR_01D05 (ggggaccactttgtacaagaaagctgggttGCGAGTAGTGACTCTTGAGGTTCCAATTTT) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列98.00%一致 | - | ||
| comment | 1st:Templateの配列とは一致 | |||
| sequence |
>IE_01D05_594bp ATGACATTAAGCGAAATCATGAAAGGAGACGATGAGCCGAAGAACAAGAGAGCTAAACATAAAGAGCTTGAGAGACAAAGAAGGCAAGAAAACACATCTC TATTCAAGATTCTAAGATATTTATTGCCATCTCAATACATAAAGGGTAAACGTTCCTCGGCAGATCACGTTCTAGAAGCAGTGAATTACATCAAAGACTT ACAGAAGAAGATCAAAGAGGTCAGCGAAAAAAGAGATAGAATCAAGAGATCTATTACTCATCCATCATCAACAGGAGAATTTTCAATAAGATCATTAGCA TCATCAACTTGTACTTGTGTTGGAGACACGCACATTGCTGTTGTGGTTAGGCCTTGTTTGATCGGACTAGAGATCGTAGTAAGTTGTTGTAACAGACACG AATCTTGTCTATCAAGTGTTCTTCAACTCCTGGCTCAAGAGCAATGCTTTAATATTGTTAGTTGCATCTCAACTAGACTACACCAAAGATTCATACACAC CATTGCTTCTGAGGTCGAGGAGGGAATAGAGGTTTATTTCTCAGAGCTTCAAGAAAAGATAATCAAAATTGGAACCTCAAGAGTCACTACTCGC >IE_01D05_translated MTLSEIMKGDDEPKNKRAKHKELERQRRQENTSLFKILRYLLPSQYIKGKRSSADHVLEAVNYIKDLQKKIKEVSEKRDRIKRSITHPSSTGEFSIRSLA SSTCTCVGDTHIAVVVRPCLIGLEIVVSCCNRHESCLSSVLQLLAQEQCFNIVSCISTRLHQRFIHTIASEVEEGIEVYFSELQEKIIKIGTSRVTTR |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 590/594 (99.3%)（アミノ酸配列で194/198 (98.0%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT1G12 ATGACATTAAGCGAAATCATGAAAGGAGACGATGAGCCGAAGAACAAGAGAGCTAAACATAAAGAGCTTGAGAGACAAAGAAGGCAAGAAAACACATCTC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01D ATGACATTAAGCGAAATCATGAAAGGAGACGATGAGCCGAAGAACAAGAGAGCTAAACATAAAGAGCTTGAGAGACAAAGAAGGCAAGAAAACACATCTC
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT1G12 TATTCAAGATTCTAAGATATTTATTGCCATCTCAATACATAAAGGGTAAACGTTCCTCGGCAGATCACGTTCTAGAAGCAGTGAATTACATCAAAGACTT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01D TATTCAAGATTCTAAGATATTTATTGCCATCTCAATACATAAAGGGTAAACGTTCCTCGGCAGATCACGTTCTAGAAGCAGTGAATTACATCAAAGACTT
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270 280 290 300
AT1G12 ACAGAAGAAGATCAAAGAGGTCAGCGAAAAAAGAGATAGAATCAAGAGATCTATTACTCATCCATCATCAAGAGGAGAATTTTCAATAAGATCATTAGCA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::
IE_01D ACAGAAGAAGATCAAAGAGGTCAGCGAAAAAAGAGATAGAATCAAGAGATCTATTACTCATCCATCATCAACAGGAGAATTTTCAATAAGATCATTAGCA
210 220 230 240 250 260 270 280 290 300
310 320 330 340 350 360 370 380 390 400
AT1G12 TCATCAACTTGTTCTTGTGTTGGAGACACGAACATTGCTGTTGTGGTTAGGCCTTGTTTGATCGGACTAGAGATCGTAGTAAGTTGTTGTAACAGACACG
:::::::::::: ::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01D TCATCAACTTGTACTTGTGTTGGAGACACGCACATTGCTGTTGTGGTTAGGCCTTGTTTGATCGGACTAGAGATCGTAGTAAGTTGTTGTAACAGACACG
310 320 330 340 350 360 370 380 390 400
410 420 430 440 450 460 470 480 490 500
AT1G12 AATCTTGTCTATCAAGTGTTCTTCAACTCCTGGCTCAAGAGCAATGCTTTAATATTGTTAGTTGCATCTCAACTAGACTACACCAAGGATTCATACACAC
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::
IE_01D AATCTTGTCTATCAAGTGTTCTTCAACTCCTGGCTCAAGAGCAATGCTTTAATATTGTTAGTTGCATCTCAACTAGACTACACCAAAGATTCATACACAC
410 420 430 440 450 460 470 480 490 500
510 520 530 540 550 560 570 580 590
AT1G12 CATTGCTTCTGAGGTCGAGGAGGGAATAGAGGTTTATTTCTCAGAGCTTCAAGAAAAGATAATCAAAATTGGAACCTCAAGAGTCACTACTCGC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01D CATTGCTTCTGAGGTCGAGGAGGGAATAGAGGTTTATTTCTCAGAGCTTCAAGAAAAGATAATCAAAATTGGAACCTCAAGAGTCACTACTCGC
510 520 530 540 550 560 570 580 590
10 20 30 40 50 60 70 80 90 100
AT1G12 MTLSEIMKGDDEPKNKRAKHKELERQRRQENTSLFKILRYLLPSQYIKGKRSSADHVLEAVNYIKDLQKKIKEVSEKRDRIKRSITHPSSRGEFSIRSLA
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::
IE_01D MTLSEIMKGDDEPKNKRAKHKELERQRRQENTSLFKILRYLLPSQYIKGKRSSADHVLEAVNYIKDLQKKIKEVSEKRDRIKRSITHPSSTGEFSIRSLA
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190
AT1G12 SSTCSCVGDTNIAVVVRPCLIGLEIVVSCCNRHESCLSSVLQLLAQEQCFNIVSCISTRLHQGFIHTIASEVEEGIEVYFSELQEKIIKIGTSRVTTR
::::.:::::.::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::
IE_01D SSTCTCVGDTHIAVVVRPCLIGLEIVVSCCNRHESCLSSVLQLLAQEQCFNIVSCISTRLHQRFIHTIASEVEEGIEVYFSELQEKIIKIGTSRVTTR
110 120 130 140 150 160 170 180 190
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