| Code name | IE_01D10 | |||
|---|---|---|---|---|
| Locus | AT5G51790 | |||
| Template | IT_01D10 (HR2498) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_01D10 (ggggacaagtttgtacaaaaaagcaggcttcATGAATCCTTCTAACAATCCTAAAAAGA) | |||
| Reverse primer | IPR_01D10 (ggggaccactttgtacaagaaagctgggttGTGCCTAGTCGTGGAAAGTACTAACTGCTC) | |||
| Alignment with template seq. | 1st | アミノ酸配列97.60%一致 | 2nd | アミノ酸配列97.60%一致 |
| Alignment with TAIR7CDS | アミノ酸配列97.60%一致 | アミノ酸配列97.60%一致 | ||
| comment | 1st:(Sequence解析まで行ったが、読み直す必要がある?), 2nd:70%, nt.178-192(15)_del | |||
| sequence |
>IE_01D10_612bp ATGAATCCTTCTAACAATCCTAAAAAGACAAGGCACCAGAGTCATATGCCTCAAGAAAGAGATGAAACGAAGAAGGAGAAGAAGCTTCTCCACCGCAACA TCGAACGACAAAGAAGACAAGAAATGGCGATCCTTTTCGCCTCTCTTCGTAGTCAGCTACCTCTAAAATACATCAAGGGAAAGAGAGCCATGTCAGATCA TGTAAACGGAGCAGTAAGTTTCATCAAAGACACGCAAACAAGGATAAAAGATCTCTCCGCAAGAAGAGACGAGCTAAAGCGAGAGATTGGTGACCCTACT AGTCTAACTGGATCCGGATCCGGATCTGGATCAAGTAGATCGGAACCAGCTAGTGTCATGGTGCAACCGTGTGTGAGCGGCTTCGAGGTGGTTGTGAGCA GCTTAGCATCAGGTCTCGAGGCTTGGCCACTCTCAAGAGTTCTTGAAGTTCTTCACGGGCAAGGACTTGAAGTCATCAGCTCTTTAACAGCACGAGTCAA CGAGAGGCTCATGTACACTATACAAGTCGAGGTAAATAGCTTTGATTGCTTCGACTTAGCTTGGTTGCAGCAGAAGCTAATTGAGCAGTTAGTACTTTCC ACGACTAGGCAC >IE_01D10_translated MNPSNNPKKTRHQSHMPQERDETKKEKKLLHRNIERQRRQEMAILFASLRSQLPLKYIKGKRAMSDHVNGAVSFIKDTQTRIKDLSARRDELKREIGDPT SLTGSGSGSGSSRSEPASVMVQPCVSGFEVVVSSLASGLEAWPLSRVLEVLHGQGLEVISSLTARVNERLMYTIQVEVNSFDCFDLAWLQQKLIEQLVLS TTRH |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 612/627 (97.6%)（アミノ酸配列で204/209 (97.6%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT5G51 ATGAATCCTTCTAACAATCCTAAAAAGACAAGGCACCAGAGTCATATGCCTCAAGAAAGAGATGAAACGAAGAAGGAGAAGAAGCTTCTCCACCGCAACA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01D ATGAATCCTTCTAACAATCCTAAAAAGACAAGGCACCAGAGTCATATGCCTCAAGAAAGAGATGAAACGAAGAAGGAGAAGAAGCTTCTCCACCGCAACA
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT5G51 TCGAACGACAAAGAAGACAAGAAATGGCGATCCTTTTCGCCTCTCTTCGTAGTCAGCTACCTCTAAAATACATCAAGGCTCTTTCTTCGCAGGGAAAGAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::
IE_01D TCGAACGACAAAGAAGACAAGAAATGGCGATCCTTTTCGCCTCTCTTCGTAGTCAGCTACCTCTAAAATACATCA---------------AGGGAAAGAG
110 120 130 140 150 160 170 180
210 220 230 240 250 260 270 280 290 300
AT5G51 AGCCATGTCAGATCATGTAAACGGAGCAGTAAGTTTCATCAAAGACACGCAAACAAGGATAAAAGATCTCTCCGCAAGAAGAGACGAGCTAAAGCGAGAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01D AGCCATGTCAGATCATGTAAACGGAGCAGTAAGTTTCATCAAAGACACGCAAACAAGGATAAAAGATCTCTCCGCAAGAAGAGACGAGCTAAAGCGAGAG
190 200 210 220 230 240 250 260 270 280
310 320 330 340 350 360 370 380 390 400
AT5G51 ATTGGTGACCCTACTAGTCTAACTGGATCCGGATCCGGATCTGGATCAAGTAGATCGGAACCAGCTAGTGTCATGGTGCAACCGTGTGTGAGCGGCTTCG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01D ATTGGTGACCCTACTAGTCTAACTGGATCCGGATCCGGATCTGGATCAAGTAGATCGGAACCAGCTAGTGTCATGGTGCAACCGTGTGTGAGCGGCTTCG
290 300 310 320 330 340 350 360 370 380
410 420 430 440 450 460 470 480 490 500
AT5G51 AGGTGGTTGTGAGCAGCTTAGCATCAGGTCTCGAGGCTTGGCCACTCTCAAGAGTTCTTGAAGTTCTTCACGGGCAAGGACTTGAAGTCATCAGCTCTTT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01D AGGTGGTTGTGAGCAGCTTAGCATCAGGTCTCGAGGCTTGGCCACTCTCAAGAGTTCTTGAAGTTCTTCACGGGCAAGGACTTGAAGTCATCAGCTCTTT
390 400 410 420 430 440 450 460 470 480
510 520 530 540 550 560 570 580 590 600
AT5G51 AACAGCACGAGTCAACGAGAGGCTCATGTACACTATACAAGTCGAGGTAAATAGCTTTGATTGCTTCGACTTAGCTTGGTTGCAGCAGAAGCTAATTGAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01D AACAGCACGAGTCAACGAGAGGCTCATGTACACTATACAAGTCGAGGTAAATAGCTTTGATTGCTTCGACTTAGCTTGGTTGCAGCAGAAGCTAATTGAG
490 500 510 520 530 540 550 560 570 580
610 620
AT5G51 CAGTTAGTACTTTCCACGACTAGGCAC
:::::::::::::::::::::::::::
IE_01D CAGTTAGTACTTTCCACGACTAGGCAC
590 600 610
10 20 30 40 50 60 70 80 90 100
AT5G51 MNPSNNPKKTRHQSHMPQERDETKKEKKLLHRNIERQRRQEMAILFASLRSQLPLKYIKALSSQGKRAMSDHVNGAVSFIKDTQTRIKDLSARRDELKRE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::
IE_01D MNPSNNPKKTRHQSHMPQERDETKKEKKLLHRNIERQRRQEMAILFASLRSQLPLKYIK-----GKRAMSDHVNGAVSFIKDTQTRIKDLSARRDELKRE
10 20 30 40 50 60 70 80 90
110 120 130 140 150 160 170 180 190 200
AT5G51 IGDPTSLTGSGSGSGSSRSEPASVMVQPCVSGFEVVVSSLASGLEAWPLSRVLEVLHGQGLEVISSLTARVNERLMYTIQVEVNSFDCFDLAWLQQKLIE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01D IGDPTSLTGSGSGSGSSRSEPASVMVQPCVSGFEVVVSSLASGLEAWPLSRVLEVLHGQGLEVISSLTARVNERLMYTIQVEVNSFDCFDLAWLQQKLIE
100 110 120 130 140 150 160 170 180 190
AT5G51 QLVLSTTRH
:::::::::
IE_01D QLVLSTTRH
200
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