| Code name | IE_01E04 | |||
|---|---|---|---|---|
| Locus | AT1G31050 | |||
| Template | IT_01E04 (HR2600) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_01E04 (ggggacaagtttgtacaaaaaagcaggcttcATGCAGCTGCTAAGCACTCCTTATATGA) | |||
| Reverse primer | IPR_01E04 (ggggaccactttgtacaagaaagctgggttACGATACAAAGAACCTCTATACGTG) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列100%一致 | - | ||
| comment | ||||
| sequence |
>IE_01E04_216bp ATGCAGCTGCTAAGCACTCCTTATATGAAGAATTCATCAATGAAGGATCCATGGGGGGGATGGGACAGAGAAGATCACAACAAAAGGGGACCGAAGCATC TTGATCTAAGGAGTAGAGGGCTTTGTTTGGTTCCTATTTCATATACCCCAATCGCATACCGCGATAACAGTGCAACTGACTACTGGAATCCCACGTATAG AGGTTCTTTGTATCGT >IE_01E04_translated MQLLSTPYMKNSSMKDPWGGWDREDHNKRGPKHLDLRSRGLCLVPISYTPIAYRDNSATDYWNPTYRGSLYR |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 141/837 (16.8%)（アミノ酸配列で15/281 ( 5.3%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT1G31 ATGGACAGGCCTGGTAGAAGCTTAGGAGATCAAATGATGGATCATCATCATCACATAGCATCAAGAAATTCATCAACAACATCAGAATTACCATCATTCG
IE_01E ----------------------------------------------------------------------------------------------------
110 120 130 140 150 160 170 180 190 200
AT1G31 AGCCAGCGTGCCATAACGGTAATGGTAACGGTTGGATCTATGACCCAAATCAAGTTAGGTACGATCAAAGTAGTGACCAACGGCTGTCAAAGTTGACGGA
IE_01E ----------------------------------------------------------------------------------------------------
210 220 230 240 250 260 270 280 290 300
AT1G31 TCTTGTAGGCAAGCACTGGTCAATTGCACCACCGAATAATCCCGACATGAACCATAACCTTCATCATCACTTCGATCATGATCATTCTCAAAACGACGAC
IE_01E ----------------------------------------------------------------------------------------------------
310 320 330 340 350 360 370 380 390 400
AT1G31 ATTTCTATGTACAGACAAGCCTTGGAGGTGAAAAATGAGGAAGATCTTTGTTACAATAATGGCTCAAGTGGTGGTGGTTCCTTGTTCCATGATCCTATAG
IE_01E ----------------------------------------------------------------------------------------------------
410 420 430 440 450 460 470 480 490
AT1G31 AAAGTTCTAGAAGTTTCCTTGATATAAGGTTAAGTAGGCCATTAACGGATATTAATCCGTCATTTAAGCCATGCTTTAAG---GCCTTAAACGTATCCGA
:: : ::: ::: :::: ::::: : :
IE_01E ------------------------------------------------------ATGC--------AGC--TGC--TAAGCACTCCTTATATG-------
10 20
500 510 520 530 540 550 560 570 580 590
AT1G31 GTTTAACAAGAAAGAA--CATCAA--ACGGCATCTCTGGCAGCAGTGAGACTGGGAACAACA-AACGCTGGAAAAAAGAAGAGATGTGAAGAAATTTCCG
::::: :::::: : :: ::: ::: : :: ::: : ::: : :: ::: ::::: :: :::
IE_01E -----------AAGAATTCATCAATGAAGG-ATCCATGGGGGGGATGGGACAGAGAAGATCACAAC-------AAAAGGGGA---------------CCG
30 40 50 60 70 80 90
600 610 620 630 640 650 660 670
AT1G31 A-------TGAGGTTTCAAAGAAGGCCAAGTGCAGT-GAGGGCT-----------CTACACTTTCGCCAGAGAAGGAACTACCCAAAGC-CAAACTTCGA
: ::: :: :: : ::: ::::::: ::: :::: : ::::: :: : :: :: ::
IE_01E AAGCATCTTGA----TCTAA-----------GGAGTAGAGGGCTTTGTTTGGTTCCTA---TTTC-----------ATATACCCCAATCGCATAC--CGC
100 110 120 130 140 150 160
680 690 700 710 720 730 740 750 760
AT1G31 GACAAGATCACGACT--CTACAGCAAATTGTGTCTCCCTTTGGAAAGACTGATACTGCTTCTGTG---CTTCAAGAGGCCATCACTTACATAAATTTTTA
:: :: : : ::: :::: : :: :::: :: ::: : :::: :: : :
IE_01E GATAACAGTGCAACTGACTACTGGAA--------TCCC---------ACGTATAGAGGTTCTTTGTATCGT-----------------------------
170 180 190 200 210
770 780 790 800
AT1G31 TCAAGAGCAAGTTAAGGTAAATTATATACATACACGG
IE_01E -------------------------------------
10 20 30 40 50 60 70 80
AT1G31 MDRPGRSLGDQMMDHHHHIASRNSSTTSELPSFEPACHNGNGNGWI-------------YDPNQVRYDQSSDQRLSKLTDLVGKHWSIAPPNNPDMNHNL
:.. . .::: .. :: : . : : : . ..: .: :: .:
IE_01E ---------MQLLSTPY---MKNSSMKDPWGGWDREDHNKRGPKHLDLRSRGLCLVPISYTPIAYRDNSATD------------YW------NPTYRGSL
10 20 30 40 50 60 70
90 100 110 120 130 140 150 160 170 180
AT1G31 HHHFDHDHSQNDDISMYRQALEVKNEEDLCYNNGSSGGGSLFHDPIESSRSFLDIRLSRPLTDINPSFKPCFKALNVSEFNKKEHQTASLAAVRLGTTNA
.
IE_01E YR--------------------------------------------------------------------------------------------------
190 200 210 220 230 240 250 260
AT1G31 GKKKRCEEISDEVSKKAKCSEGSTLSPEKELPKAKLRDKITTLQQIVSPFGKTDTASVLQEAITYINFYQEQVKVNYIHTR
IE_01E ---------------------------------------------------------------------------------
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