| Code name | IE_01G02 | |||
|---|---|---|---|---|
| Locus | AT5G05120 | |||
| Template | IT_01G02 (HR3493) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_01G02 (ggggacaagtttgtacaaaaaagcaggcttcATGTTGTCCTCGGACTCGAATTACGCTA) | |||
| Reverse primer | IPR_01G02 (ggggaccactttgtacaagaaagctgggttCAGTTTGAGTGAGAGAGAAAGGGAAGAACC) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列100%一致 | - | ||
| comment | 1st:Templateの配列とは一致 | |||
| sequence |
>IE_01G02_603bp ATGTTGTCCTCGGACTCGAATTACGCTAGTGATATTAGCGACGATGCCTCCGCCACCGGATCGATAGAGAATCCTATATACAAATGCAAGTATTGTCCTA GGAAGTTCGATAAAACACAAGCATTAGGTGGTCATCAAAATGCACACAGAAAGGAGAGAGAGGTCGAAAAACAACAAAAAGCATTTTTGGCGCATTTGAA CCGACCAGAACCAGATCTTTACGCGTACTCGTATTCGTATCATCATTCATTTCCTAACCAATACGCACTCCCACCGGGATTTGAACAGCCTCAGTACAAA GTTGATAGATCATACAAGATGTCCATGGTCTACAACCAATATGTGGGATCCTCAAGCTCTAGCTTTGCAGGACTACAAAGTGACCCAAGTCAAGGAATGA ACCAGGATTGGACCTTTACCGGGATCCCATTCCTACCCCAATCTCAACCTCAACCACTATCGTCACCAATATGTTTGGATCTTTGCCTTGGCATTGGTAG CTCCCAAACCCAGCCACAACCTCAAGAACCAAATGATGCAACAGAAGAGATGGATGCTGAGAAAGAAAATGATGGTTCTTCCCTTTCTCTCTCACTCAAA CTG >IE_01G02_translated MLSSDSNYASDISDDASATGSIENPIYKCKYCPRKFDKTQALGGHQNAHRKEREVEKQQKAFLAHLNRPEPDLYAYSYSYHHSFPNQYALPPGFEQPQYK VDRSYKMSMVYNQYVGSSSSSFAGLQSDPSQGMNQDWTFTGIPFLPQSQPQPLSSPICLDLCLGIGSSQTQPQPQEPNDATEEMDAEKENDGSSLSLSLK L |
|||
| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 602/603 (99.8%)（アミノ酸配列で100%）一致しました。
10 20 30 40 50 60 70 80 90 100
AT5G05 ATGTTGTCCTCGGACTCGAATTACGCTAGTGATATTAGCGACGATGCCTCCGCCACCGGATCGATAGAGAATCCTATATACAAATGCAAGTATTGTCCTA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G ATGTTGTCCTCGGACTCGAATTACGCTAGTGATATTAGCGACGATGCCTCCGCCACCGGATCGATAGAGAATCCTATATACAAATGCAAGTATTGTCCTA
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT5G05 GGAAGTTCGATAAAACACAAGCATTAGGTGGTCATCAAAATGCACACAGAAAGGAGAGAGAGGTCGAAAAACAACAAAAAGCATTTTTGGCGCATTTGAA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G GGAAGTTCGATAAAACACAAGCATTAGGTGGTCATCAAAATGCACACAGAAAGGAGAGAGAGGTCGAAAAACAACAAAAAGCATTTTTGGCGCATTTGAA
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270 280 290 300
AT5G05 CCGACCAGAACCAGATCTTTACGCGTACTCGTATTCGTATCATCATTCATTTCCTAACCAATACGCACTCCCACCGGGATTTGAACAGCCTCAGTACAAA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G CCGACCAGAACCAGATCTTTACGCGTACTCGTATTCGTATCATCATTCATTTCCTAACCAATACGCACTCCCACCGGGATTTGAACAGCCTCAGTACAAA
210 220 230 240 250 260 270 280 290 300
310 320 330 340 350 360 370 380 390 400
AT5G05 GTTGATAGATCATACAAGATGTCCATGGTCTACAACCAATATGTGGGATCCTCAAGCTCTAGCTTTGCAGGACTACAAAGTGACCCAAGTCAAGGAATGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G GTTGATAGATCATACAAGATGTCCATGGTCTACAACCAATATGTGGGATCCTCAAGCTCTAGCTTTGCAGGACTACAAAGTGACCCAAGTCAAGGAATGA
310 320 330 340 350 360 370 380 390 400
410 420 430 440 450 460 470 480 490 500
AT5G05 ACCAGGATTGGACCTTTACCGGGATCCCATTCCTACCCCAATCTCAACCTCAACCACTATCGTCACCAATATGTTTGGATCTTTGCCTTGGCATTGGTAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G ACCAGGATTGGACCTTTACCGGGATCCCATTCCTACCCCAATCTCAACCTCAACCACTATCGTCACCAATATGTTTGGATCTTTGCCTTGGCATTGGTAG
410 420 430 440 450 460 470 480 490 500
510 520 530 540 550 560 570 580 590 600
AT5G05 CTCCCAAACCCAACCACAACCTCAAGAACCAAATGATGCAACAGAAGAGATGGATGCTGAGAAAGAAAATGATGGTTCTTCCCTTTCTCTCTCACTCAAA
:::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G CTCCCAAACCCAGCCACAACCTCAAGAACCAAATGATGCAACAGAAGAGATGGATGCTGAGAAAGAAAATGATGGTTCTTCCCTTTCTCTCTCACTCAAA
510 520 530 540 550 560 570 580 590 600
AT5G05 CTG
:::
IE_01G CTG
10 20 30 40 50 60 70 80 90 100
AT5G05 MLSSDSNYASDISDDASATGSIENPIYKCKYCPRKFDKTQALGGHQNAHRKEREVEKQQKAFLAHLNRPEPDLYAYSYSYHHSFPNQYALPPGFEQPQYK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G MLSSDSNYASDISDDASATGSIENPIYKCKYCPRKFDKTQALGGHQNAHRKEREVEKQQKAFLAHLNRPEPDLYAYSYSYHHSFPNQYALPPGFEQPQYK
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT5G05 VDRSYKMSMVYNQYVGSSSSSFAGLQSDPSQGMNQDWTFTGIPFLPQSQPQPLSSPICLDLCLGIGSSQTQPQPQEPNDATEEMDAEKENDGSSLSLSLK
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G VDRSYKMSMVYNQYVGSSSSSFAGLQSDPSQGMNQDWTFTGIPFLPQSQPQPLSSPICLDLCLGIGSSQTQPQPQEPNDATEEMDAEKENDGSSLSLSLK
110 120 130 140 150 160 170 180 190 200
AT5G05 L
:
IE_01G L
|
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