| Code name | IE_01G05 | |||
|---|---|---|---|---|
| Locus | AT1G47655 | |||
| Template | IT_01G05 (HR3521) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_01G05 (ggggacaagtttgtacaaaaaagcaggcttcATGCCGTCGGAACCAAACCAAACCCGAC) | |||
| Reverse primer | IPR_01G05 (ggggaccactttgtacaagaaagctgggttCTTAACATCGTTGGTTTGCATTGAGATCTC) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列100%一致 | - | ||
| comment | 1st:Templateの配列とは一致 | |||
| sequence |
>IE_01G05_627bp ATGCCGTCGGAACCAAACCAAACCCGACCCACCAGAGTTCAGCCCTCAACGGCGGCTTACCCACCGCCAAATCTGGCTGAGCCTCTTCCTTGTCCTCGCT GCAACTCCACCACCACCAAGTTCTGTTACTACAACAACTATAACCTCGCTCAGCCTCGCTACTACTGCAAATCTTGCCGCCGTTACTGGACTCAAGGTGG TACACTCCGTGACGTCCCCGTCGGTGGTGGAACTCGTCGAAGCTCCTCAAAACGTCACCGTTCTTTCTCCACCACTGCCACCTCCTCTTCCTCCTCTTCT TCCGTCATCACCACCACGACACAAGAACCAGCCACGACTGAAGCGAGTCAAACTAAGGTTACTAATTTAATTTCAGGTCATGGAAGCTTTGCTTCTCTGT TAGGTTTAGGAAGTGGAAATGGTGGGTTGGATTACGGGTTTGGGTACGGGTACGGGCTTGAGGAGATGAGTATTGGGTATCTTGGAGATTCTTCCGTAGG AGAGATTCCGGTGGTTGATGGTTGTGGTGGTGACACGTGGCAGATTGGGGAGATTGAAGGTAAAAGTGGGGGAGACAGTTTGATATGGCCTGGTCTTGAG ATCTCAATGCAAACCAACGATGTTAAG >IE_01G05_translated MPSEPNQTRPTRVQPSTAAYPPPNLAEPLPCPRCNSTTTKFCYYNNYNLAQPRYYCKSCRRYWTQGGTLRDVPVGGGTRRSSSKRHRSFSTTATSSSSSS SVITTTTQEPATTEASQTKVTNLISGHGSFASLLGLGSGNGGLDYGFGYGYGLEEMSIGYLGDSSVGEIPVVDGCGGDTWQIGEIEGKSGGDSLIWPGLE ISMQTNDVK |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 626/627 (99.8%)（アミノ酸配列で100%）一致しました。
10 20 30 40 50 60 70 80 90 100
AT1G47 ATGCCGTCGGAACCAAACCAAACCCGACCCACCAGAGTTCAGCCCTCAACGGCGGCTTACCCACCGCCAAATCTGGCTGAGCCTCTTCCTTGTCCTCGCT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G ATGCCGTCGGAACCAAACCAAACCCGACCCACCAGAGTTCAGCCCTCAACGGCGGCTTACCCACCGCCAAATCTGGCTGAGCCTCTTCCTTGTCCTCGCT
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT1G47 GCAACTCCACCACCACCAAGTTCTGTTACTACAACAACTATAACCTCGCTCAGCCTCGCTACTACTGCAAATCTTGCCGCCGTTACTGGACTCAAGGTGG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G GCAACTCCACCACCACCAAGTTCTGTTACTACAACAACTATAACCTCGCTCAGCCTCGCTACTACTGCAAATCTTGCCGCCGTTACTGGACTCAAGGTGG
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270 280 290 300
AT1G47 TACACTCCGTGACGTCCCCGTCGGTGGTGGAACTCGTCGAAGCTCCTCAAAACGTCACCGTTCTTTCTCCACCACTGCCACCTCCTCTTCCTCCTCTTCT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G TACACTCCGTGACGTCCCCGTCGGTGGTGGAACTCGTCGAAGCTCCTCAAAACGTCACCGTTCTTTCTCCACCACTGCCACCTCCTCTTCCTCCTCTTCT
210 220 230 240 250 260 270 280 290 300
310 320 330 340 350 360 370 380 390 400
AT1G47 TCCGTCATCACCACCACGACACAAGAACCAGCCACGACTGAAGCGAGTCAAACTAAGGTTACTAATTTAATTTCAGGTCATGGAAGCTTTGCTTCTCTGT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G TCCGTCATCACCACCACGACACAAGAACCAGCCACGACTGAAGCGAGTCAAACTAAGGTTACTAATTTAATTTCAGGTCATGGAAGCTTTGCTTCTCTGT
310 320 330 340 350 360 370 380 390 400
410 420 430 440 450 460 470 480 490 500
AT1G47 TAGGTTTAGGAAGTGGAAATGGTGGGTTGGATTACGGGTTTGGGTACGGGTACGGGCTTGAGGAGATGAGTATTGGGTATCTTGGAGATTCTTCCGTAGG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G TAGGTTTAGGAAGTGGAAATGGTGGGTTGGATTACGGGTTTGGGTACGGGTACGGGCTTGAGGAGATGAGTATTGGGTATCTTGGAGATTCTTCCGTAGG
410 420 430 440 450 460 470 480 490 500
510 520 530 540 550 560 570 580 590 600
AT1G47 AGAGATTCCGGTGGTTGATGGTTGTGGTGGTGACACGTGGCAGATTGGGGAGATTGAAGGTAAAAGTGGAGGAGACAGTTTGATATGGCCTGGTCTTGAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::
IE_01G AGAGATTCCGGTGGTTGATGGTTGTGGTGGTGACACGTGGCAGATTGGGGAGATTGAAGGTAAAAGTGGGGGAGACAGTTTGATATGGCCTGGTCTTGAG
510 520 530 540 550 560 570 580 590 600
610 620
AT1G47 ATCTCAATGCAAACCAACGATGTTAAG
:::::::::::::::::::::::::::
IE_01G ATCTCAATGCAAACCAACGATGTTAAG
610 620
10 20 30 40 50 60 70 80 90 100
AT1G47 MPSEPNQTRPTRVQPSTAAYPPPNLAEPLPCPRCNSTTTKFCYYNNYNLAQPRYYCKSCRRYWTQGGTLRDVPVGGGTRRSSSKRHRSFSTTATSSSSSS
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G MPSEPNQTRPTRVQPSTAAYPPPNLAEPLPCPRCNSTTTKFCYYNNYNLAQPRYYCKSCRRYWTQGGTLRDVPVGGGTRRSSSKRHRSFSTTATSSSSSS
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT1G47 SVITTTTQEPATTEASQTKVTNLISGHGSFASLLGLGSGNGGLDYGFGYGYGLEEMSIGYLGDSSVGEIPVVDGCGGDTWQIGEIEGKSGGDSLIWPGLE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01G SVITTTTQEPATTEASQTKVTNLISGHGSFASLLGLGSGNGGLDYGFGYGYGLEEMSIGYLGDSSVGEIPVVDGCGGDTWQIGEIEGKSGGDSLIWPGLE
110 120 130 140 150 160 170 180 190 200
AT1G47 ISMQTNDVK
:::::::::
IE_01G ISMQTNDVK
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