| Code name | IE_01H06 | |||
|---|---|---|---|---|
| Locus | AT1G77570 | |||
| Template | IT_01H06 (HR3744) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_01H06 (ggggacaagtttgtacaaaaaagcaggcttcATGGGTTACAATTTCTACATGAGAGTGT) | |||
| Reverse primer | IPR_01H06 (ggggaccactttgtacaagaaagctgggttAATTTGCAAGTGTTGGAAGAGAAACTCGAC) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列85.00%一致 | - | ||
| comment | 1st:Templateの配列とは一致 | |||
| sequence |
>IE_01H06_375bp ATGGGTTACAATTTCTACATGAGAGTGTATGAGGTTGTTGATGATGCTTCAACGGATGCAATCATATCGTGGAGCGAAAGCAACAACAGTTTCATTATCT GGAATGTTGGAGAGTTTTACAGAAGGATTCTGCCTAAATATGTAGACTTGGGCACAAACCTCTCACGGTTTTTCTCCAACCTTCGTTCTCATGGGTTCAA AATAGTTAAAGGAAGAACTGGAGTATTGGAATTTGGACATGAAGATTTTATCAGAGATAAACTGGAGCTTATGAAGAAGATGGTTAGCGATAAACGTAAA GCAAGGAAAGCTGCTAAATCCAAAGCAAGGAAAGCTAGAGTTCAAGTCGAGTTTCTCTTCCAACACTTGCAAATT >IE_01H06_translated MGYNFYMRVYEVVDDASTDAIISWSESNNSFIIWNVGEFYRRILPKYVDLGTNLSRFFSNLRSHGFKIVKGRTGVLEFGHEDFIRDKLELMKKMVSDKRK ARKAAKSKARKARVQVEFLFQHLQI |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 375/441 (85.0%)（アミノ酸配列で125/147 (85.0%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT1G77 ATGCTAAAATCTCGAAACCCTAATTATTCTAAGATTCTAATTTGTGAAGTTTGTGAGGTTTTAGGGATGGGTTACAATTTCTACATGAGAGTGTATGAGG
::::::::::::::::::::::::::::::::::
IE_01H ------------------------------------------------------------------ATGGGTTACAATTTCTACATGAGAGTGTATGAGG
10 20 30
110 120 130 140 150 160 170 180 190 200
AT1G77 TTGTTGATGATGCTTCAACGGATGCAATCATATCGTGGAGCGAAAGCAACAACAGTTTCATTATCTGGAATGTTGGAGAGTTTTACAGAAGGATTCTGCC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01H TTGTTGATGATGCTTCAACGGATGCAATCATATCGTGGAGCGAAAGCAACAACAGTTTCATTATCTGGAATGTTGGAGAGTTTTACAGAAGGATTCTGCC
40 50 60 70 80 90 100 110 120 130
210 220 230 240 250 260 270 280 290 300
AT1G77 TAAATATGTAGACTTGGGCACAAACCTCTCACGGTTTTTCTCCAACCTTCGTTCTCATGGGTTCAAAATAGTTAAAGGAAGAACTGGAGTATTGGAATTT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01H TAAATATGTAGACTTGGGCACAAACCTCTCACGGTTTTTCTCCAACCTTCGTTCTCATGGGTTCAAAATAGTTAAAGGAAGAACTGGAGTATTGGAATTT
140 150 160 170 180 190 200 210 220 230
310 320 330 340 350 360 370 380 390 400
AT1G77 GGACATGAAGATTTTATCAGAGATAAACTGGAGCTTATGAAGAAGATGGTTAGCGATAAACGTAAAGCAAGGAAAGCTGCTAAATCCAAAGCAAGGAAAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01H GGACATGAAGATTTTATCAGAGATAAACTGGAGCTTATGAAGAAGATGGTTAGCGATAAACGTAAAGCAAGGAAAGCTGCTAAATCCAAAGCAAGGAAAG
240 250 260 270 280 290 300 310 320 330
410 420 430 440
AT1G77 CTAGAGTTCAAGTCGAGTTTCTCTTCCAACACTTGCAAATT
:::::::::::::::::::::::::::::::::::::::::
IE_01H CTAGAGTTCAAGTCGAGTTTCTCTTCCAACACTTGCAAATT
340 350 360 370
10 20 30 40 50 60 70 80 90 100
AT1G77 MLKSRNPNYSKILICEVCEVLGMGYNFYMRVYEVVDDASTDAIISWSESNNSFIIWNVGEFYRRILPKYVDLGTNLSRFFSNLRSHGFKIVKGRTGVLEF
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_01H ----------------------MGYNFYMRVYEVVDDASTDAIISWSESNNSFIIWNVGEFYRRILPKYVDLGTNLSRFFSNLRSHGFKIVKGRTGVLEF
10 20 30 40 50 60 70
110 120 130 140
AT1G77 GHEDFIRDKLELMKKMVSDKRKARKAAKSKARKARVQVEFLFQHLQI
:::::::::::::::::::::::::::::::::::::::::::::::
IE_01H GHEDFIRDKLELMKKMVSDKRKARKAAKSKARKARVQVEFLFQHLQI
80 90 100 110 120
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