◆◆◆IE_02A11 detail◆◆◆

Code name	IE_02A11
Locus	AT4G21080
Template	IT_02A11 (HR1393)
Donor Vector	pDONR207
Forward primer	IPF_02A11 (ggggacaagtttgtacaaaaaagcaggcttcATGGATAACTTGAATGTTTTCGCAAATG)
Reverse primer	IPR_02A11 (ggggaccactttgtacaagaaagctgggttAGGACCATCCTTCTCCAGATGACAAGGATG)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.60%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_02A11_747bp ATGGATAACTTGAATGTTTTCGCAAATGAAGACAATCAAGTGAATGATGTGAAGCTCCCACCACCACCACCTCGAGTGTGTGCAAGGTGTGATTCTGATA ATACTAAATTTTGTTATTACAACAACTACTGTGAGTTTCAGCCACGATACTTCTGCAAGAACTGTCGTAGATACTGGACTCATGGTGGGGCTTTAAGAAA CATACCAATTGGTGGAAGTAGTCGTGCCAAACGGGCAAGGGTAAATCAACCTTCGGTTGCTCGGATGGTTTCTGTTGAGACCCAACGAGGTAACAATCAA CCTTTCTCTAATGTTCAAGAAAACGTTCATCTTGTTGGATCTTTTGGTGCTTCATCTTCATCTTCTGTTGGTGCTGTTGGGAACCTTTTTGGTTCTTTGT ATGATATTCATGGTGGTATGGTAACAAATTTGCATCCAACTCGAACTGTTCGACCAAATCATCGCTTAGCTTTCCATGATGGATCATTTGAGCAAGACTA TTACGATGTTGGGTCCGATAATCTTTTGGTCAACCAACAAGTTGGTGGCTACGGTTATCACATGAATCCAGTGGATCAATTCAAGTGGAACCAGAGCTTC AACAACACTATGAACATGAATTATAATAACGATAGCACTAGTGGAAGTAGCAGAGGATCTGACATGAATGTGAACCATGATAACAAGAAGATCAGATACC GCAACTCTGTGATTATGCATCCTTGTCATCTGGAGAAGGATGGTCCT >IE_02A11_translated MDNLNVFANEDNQVNDVKLPPPPPRVCARCDSDNTKFCYYNNYCEFQPRYFCKNCRRYWTHGGALRNIPIGGSSRAKRARVNQPSVARMVSVETQRGNNQ PFSNVQENVHLVGSFGASSSSSVGAVGNLFGSLYDIHGGMVTNLHPTRTVRPNHRLAFHDGSFEQDYYDVGSDNLLVNQQVGGYGYHMNPVDQFKWNQSF NNTMNMNYNNDSTSGSSRGSDMNVNHDNKKIRYRNSVIMHPCHLEKDGP
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 746/747 (99.9%)（アミノ酸配列で248/249 (99.6%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G21 ATGGATAACTTGAATGTTTTCGCAAATGAAGACAATCAAGTGAATGATGTGAAGCCCCCACCACCACCACCTCGAGTGTGTGCAAGGTGTGATTCTGATA ::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::: IE_02A ATGGATAACTTGAATGTTTTCGCAAATGAAGACAATCAAGTGAATGATGTGAAGCTCCCACCACCACCACCTCGAGTGTGTGCAAGGTGTGATTCTGATA 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G21 ATACTAAATTTTGTTATTACAACAACTACTGTGAGTTTCAGCCACGATACTTCTGCAAGAACTGTCGTAGATACTGGACTCATGGTGGGGCTTTAAGAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02A ATACTAAATTTTGTTATTACAACAACTACTGTGAGTTTCAGCCACGATACTTCTGCAAGAACTGTCGTAGATACTGGACTCATGGTGGGGCTTTAAGAAA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G21 CATACCAATTGGTGGAAGTAGTCGTGCCAAACGGGCAAGGGTAAATCAACCTTCGGTTGCTCGGATGGTTTCTGTTGAGACCCAACGAGGTAACAATCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02A CATACCAATTGGTGGAAGTAGTCGTGCCAAACGGGCAAGGGTAAATCAACCTTCGGTTGCTCGGATGGTTTCTGTTGAGACCCAACGAGGTAACAATCAA 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT4G21 CCTTTCTCTAATGTTCAAGAAAACGTTCATCTTGTTGGATCTTTTGGTGCTTCATCTTCATCTTCTGTTGGTGCTGTTGGGAACCTTTTTGGTTCTTTGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02A CCTTTCTCTAATGTTCAAGAAAACGTTCATCTTGTTGGATCTTTTGGTGCTTCATCTTCATCTTCTGTTGGTGCTGTTGGGAACCTTTTTGGTTCTTTGT 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT4G21 ATGATATTCATGGTGGTATGGTAACAAATTTGCATCCAACTCGAACTGTTCGACCAAATCATCGCTTAGCTTTCCATGATGGATCATTTGAGCAAGACTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02A ATGATATTCATGGTGGTATGGTAACAAATTTGCATCCAACTCGAACTGTTCGACCAAATCATCGCTTAGCTTTCCATGATGGATCATTTGAGCAAGACTA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT4G21 TTACGATGTTGGGTCCGATAATCTTTTGGTCAACCAACAAGTTGGTGGCTACGGTTATCACATGAATCCAGTGGATCAATTCAAGTGGAACCAGAGCTTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02A TTACGATGTTGGGTCCGATAATCTTTTGGTCAACCAACAAGTTGGTGGCTACGGTTATCACATGAATCCAGTGGATCAATTCAAGTGGAACCAGAGCTTC 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT4G21 AACAACACTATGAACATGAATTATAATAACGATAGCACTAGTGGAAGTAGCAGAGGATCTGACATGAATGTGAACCATGATAACAAGAAGATCAGATACC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02A AACAACACTATGAACATGAATTATAATAACGATAGCACTAGTGGAAGTAGCAGAGGATCTGACATGAATGTGAACCATGATAACAAGAAGATCAGATACC 610 620 630 640 650 660 670 680 690 700 710 720 730 740 AT4G21 GCAACTCTGTGATTATGCATCCTTGTCATCTGGAGAAGGATGGTCCT ::::::::::::::::::::::::::::::::::::::::::::::: IE_02A GCAACTCTGTGATTATGCATCCTTGTCATCTGGAGAAGGATGGTCCT 710 720 730 740 10 20 30 40 50 60 70 80 90 100 AT4G21 MDNLNVFANEDNQVNDVKPPPPPPRVCARCDSDNTKFCYYNNYCEFQPRYFCKNCRRYWTHGGALRNIPIGGSSRAKRARVNQPSVARMVSVETQRGNNQ :::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02A MDNLNVFANEDNQVNDVKLPPPPPRVCARCDSDNTKFCYYNNYCEFQPRYFCKNCRRYWTHGGALRNIPIGGSSRAKRARVNQPSVARMVSVETQRGNNQ 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G21 PFSNVQENVHLVGSFGASSSSSVGAVGNLFGSLYDIHGGMVTNLHPTRTVRPNHRLAFHDGSFEQDYYDVGSDNLLVNQQVGGYGYHMNPVDQFKWNQSF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02A PFSNVQENVHLVGSFGASSSSSVGAVGNLFGSLYDIHGGMVTNLHPTRTVRPNHRLAFHDGSFEQDYYDVGSDNLLVNQQVGGYGYHMNPVDQFKWNQSF 110 120 130 140 150 160 170 180 190 200 210 220 230 240 AT4G21 NNTMNMNYNNDSTSGSSRGSDMNVNHDNKKIRYRNSVIMHPCHLEKDGP ::::::::::::::::::::::::::::::::::::::::::::::::: IE_02A NNTMNMNYNNDSTSGSSRGSDMNVNHDNKKIRYRNSVIMHPCHLEKDGP 210 220 230 240

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