◆◆◆IE_02B12 detail◆◆◆

Code name	IE_02B12
Locus	AT4G35900
Template	IT_02B12 (HR1935)
Donor Vector	pDONR207
Forward primer	IPF_02B12 (ggggacaagtttgtacaaaaaagcaggcttcATGTTGTCATCAGCTAAGCATCAGAGAA)
Reverse primer	IPR_02B12 (ggggaccactttgtacaagaaagctgggttAAATGGAGCTGTGGAAGACCGTTGAAGTGT)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列82.80%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_02B12_855bp ATGTTGTCATCAGCTAAGCATCAGAGAAACCATAGACTCTCTGCTACAAACAAGAACCAGACTCTCACCAAAGTTTCTTCCATTTCATCCTCATCACCAT CGTCTTCTTCTTCATCATCATCAACCTCATCATCATCTCCTTTACCTTCTCAAGACTCTCAAGCCCAGAAGAGATCTCTTGTCACCATGGAAGAAGTTTG GAATGACATCAACCTTGCTTCCATCCACCACCTAAACCGACACAGCCCTCATCCACAACACAACCACGAGCCAAGGTTCAGGGGCCAAAACCACCACAAC CAAAACCCTAACTCAATCTTCCAAGATTTTCTCAAAGGATCTTTGAACCAGGAACCAGCACCCACAAGCCAGACCACGGGTTCTGCGCCTAATGGCGATT CCACCACGGTCACTGTTCTTTACAGCTCTCCTTTTCCACCTCCTGCAACTGTTCTGAGCTTGAATTCCGGCGCTGGCTTCGAGTTTCTCGATAACCAAGA TCCTCTTGTTACCTCAAACTCTAATCTTCATACCCACCATCACCTCTCAAACGCTCATGCCTTCAACACCTCTTTCGAGGCTCTGGTTCCATCCAGTTCT TTTGGTAAGAAAAGAGGCCAAGATTCCAATGAAGGTTCAGGGAATAGAAGACATAAGCGTATGATCAAGAACAGAGAATCTGCAGCTCGTTCCCGCGCTA GGAAACAGGCTTATACAAACGAGTTAGAACTTGAAGTTGCTCACTTGCAGGCAGAAAATGCAAGACTCAAGAGACAACAAGATCAGTTAAAAATGGCTGC AGCAATTCAGCAACCCAAAAAGAACACACTTCAACGGTCTTCCACAGCTCCATTT >IE_02B12_translated MLSSAKHQRNHRLSATNKNQTLTKVSSISSSSPSSSSSSSSTSSSSPLPSQDSQAQKRSLVTMEEVWNDINLASIHHLNRHSPHPQHNHEPRFRGQNHHN QNPNSIFQDFLKGSLNQEPAPTSQTTGSAPNGDSTTVTVLYSSPFPPPATVLSLNSGAGFEFLDNQDPLVTSNSNLHTHHHLSNAHAFNTSFEALVPSSS FGKKRGQDSNEGSGNRRHKRMIKNRESAARSRARKQAYTNELELEVAHLQAENARLKRQQDQLKMAAAIQQPKKNTLQRSSTAPF
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 731/859 (85.1%)（アミノ酸配列で237/291 (81.4%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G35 ATGTTGTCATCAGCTAAGCATCAGAGAAACCATAGACTCTCTGCTACAAACAAGAACCAGACTCTCACCAAAGTTTCTTCCATTTCATCCTCATCACCAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02B ATGTTGTCATCAGCTAAGCATCAGAGAAACCATAGACTCTCTGCTACAAACAAGAACCAGACTCTCACCAAAGTTTCTTCCATTTCATCCTCATCACCAT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G35 CGTCTTCTTCTTCATCATCATCAACCTCATCATCATCTCCTTTACCTTCTCAAGACTCTCAAGCCCAGAAGAGATCTCTTGTCACCATGGAAGAAGTTTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02B CGTCTTCTTCTTCATCATCATCAACCTCATCATCATCTCCTTTACCTTCTCAAGACTCTCAAGCCCAGAAGAGATCTCTTGTCACCATGGAAGAAGTTTG 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G35 GAATGACATCAACCTTGCTTCCATCCACCACCTAAACCGACACAGCCCTCATCCACAACACAACCACGAGCCAAGGTTCAGGGGCCAAAACCACCACAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02B GAATGACATCAACCTTGCTTCCATCCACCACCTAAACCGACACAGCCCTCATCCACAACACAACCACGAGCCAAGGTTCAGGGGCCAAAACCACCACAAC 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT4G35 CAAAACCCTAACTCAATCTTCCAAGATTTTCTCAAAGGATCTTTGAACCAGGAACCAGCACCCACAAGCCAGACCACGGGTTCTGCGCCTAATGGCGATT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02B CAAAACCCTAACTCAATCTTCCAAGATTTTCTCAAAGGATCTTTGAACCAGGAACCAGCACCCACAAGCCAGACCACGGGTTCTGCGCCTAATGGCGATT 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT4G35 CCACCACGGTCACTGTTCTTTACAGCTCTCCTTTTCCACCTCCTGCAACTGTTCTGAGCTTGAATTCCGGCGCTGGCTTCGAGTTTCTCGATAACCAAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02B CCACCACGGTCACTGTTCTTTACAGCTCTCCTTTTCCACCTCCTGCAACTGTTCTGAGCTTGAATTCCGGCGCTGGCTTCGAGTTTCTCGATAACCAAGA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT4G35 TCCTCTTGTTACCTCAAACTCTAATCTTCATACCCACCATCACCTCTCAAACGCTCATGCCTTCAACACCTCTTTCGAGGCTCTGGTTCCATCCAGTTCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02B TCCTCTTGTTACCTCAAACTCTAATCTTCATACCCACCATCACCTCTCAAACGCTCATGCCTTCAACACCTCTTTCGAGGCTCTGGTTCCATCCAGTTCT 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT4G35 TTTGGTAAGAAAAGAGGCCAAGATTCCAATGAAGGTTCAGGGAATAGAAGACATAAGCGTATGATCAAGAACAGAGAATCTGCAGCTCGTTCCCGCGCTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02B TTTGGTAAGAAAAGAGGCCAAGATTCCAATGAAGGTTCAGGGAATAGAAGACATAAGCGTATGATCAAGAACAGAGAATCTGCAGCTCGTTCCCGCGCTA 610 620 630 640 650 660 670 680 690 700 710 720 730 AT4G35 GGAAACAGGAATGCTTATACAAACGAGTTAGAACT----------------------------------------------------------------- :::::::: ::::::::::::::::::::::: IE_02B GGAAACAG----GCTTATACAAACGAGTTAGAACTTGAAGTTGCTCACTTGCAGGCAGAAAATGCAAGACTCAAGAGACAACAAGATCAGTTAAAAATGG 710 720 730 740 750 760 770 780 790 AT4G35 ----------------------------------------------------------- IE_02B CTGCAGCAATTCAGCAACCCAAAAAGAACACACTTCAACGGTCTTCCACAGCTCCATTT 800 810 820 830 840 850 10 20 30 40 50 60 70 80 90 100 AT4G35 MLSSAKHQRNHRLSATNKNQTLTKVSSISSSSPSSSSSSSSTSSSSPLPSQDSQAQKRSLVTMEEVWNDINLASIHHLNRHSPHPQHNHEPRFRGQNHHN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02B MLSSAKHQRNHRLSATNKNQTLTKVSSISSSSPSSSSSSSSTSSSSPLPSQDSQAQKRSLVTMEEVWNDINLASIHHLNRHSPHPQHNHEPRFRGQNHHN 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G35 QNPNSIFQDFLKGSLNQEPAPTSQTTGSAPNGDSTTVTVLYSSPFPPPATVLSLNSGAGFEFLDNQDPLVTSNSNLHTHHHLSNAHAFNTSFEALVPSSS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02B QNPNSIFQDFLKGSLNQEPAPTSQTTGSAPNGDSTTVTVLYSSPFPPPATVLSLNSGAGFEFLDNQDPLVTSNSNLHTHHHLSNAHAFNTSFEALVPSSS 110 120 130 140 150 160 170 180 190 200 210 220 230 240 AT4G35 FGKKRGQDSNEGSGNRRHKRMIKNRESAARSRARKQECLYKRVRT---------------------------------------------- :::::::::::::::::::::::::::::::::::: : IE_02B FGKKRGQDSNEGSGNRRHKRMIKNRESAARSRARKQ------AYTNELELEVAHLQAENARLKRQQDQLKMAAAIQQPKKNTLQRSSTAPF 210 220 230 240 250 260 270 280

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