◆◆◆IE_02C04 detail◆◆◆

Code name	IE_02C04
Locus	AT4G13480
Template	IT_02C04 (HR2071)
Donor Vector	pDONR207
Forward primer	IPF_02C04 (ggggacaagtttgtacaaaaaagcaggcttcATGGTGGAAGAAGTTTGGAGAAAGGGTC)
Reverse primer	IPR_02C04 (ggggaccactttgtacaagaaagctgggttACAAAATGGAATCACCAAGTTATGGAAATA)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.60%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_02C04_783bp ATGGTGGAAGAAGTTTGGAGAAAGGGTCCATGGACCGCCGAAGAAGACCGTCTTTTGATCGAATACGTCCGTGTTCACGGTGAAGGTCGTTGGAACTCTG TCTCTAAACTCGCAGGATTGAAAAGGAATGGCAAAAGCTGCAGACTAAGATGGGTGAATTACCTTAGACCAGACCTCAAGAGAGGACAGATCACTCCACA TGAAGAAAGTATAATACTTGAGCTACACGCTAAGTGGGGAAATAGGTGGTCAACAATTGCACGTAGTTTACCAGGAAGAACAGACAATGAGATCAAGAAC TATTGGAGAACCCATTTCAAGAAAAAGGCAAAGCCTACGACTAACAATGCGGAGAAGATAAAGAGTCGTCTCCTAAAAAGGCAACACTTCAAGGAACAGA GAGAAATAGAGTTGCAACAAGAACAGCAGTTGTTTCAGTTCGACCAACTCGGTATGAAAAAGATCATCTCTTTACTCGAAGAAAACAATAGCAGTAGCAG TAGCGATGGCGGTGGTGATGTGTTCTATTATCCTGATCAAATAACACATTCACCAAAACCCTTTGGCTATAACTCTAATTCATTAGAGGAGCAGTTACAA GGTAGATTTTCTCCTGTAAACATACCTGATGCTAATACTATGAACGAAGACAATGCCATATGGGACGGGTTTTGGAACATGGATGTTGTAAATGGACATG GTGGGAACTTGGGTGTTGTGGCTGCTACTGCTGCTTGTGGCCCAAGGAAGCCCTATTTCCATAACTTGGTGATTCCATTTTGT >IE_02C04_translated MVEEVWRKGPWTAEEDRLLIEYVRVHGEGRWNSVSKLAGLKRNGKSCRLRWVNYLRPDLKRGQITPHEESIILELHAKWGNRWSTIARSLPGRTDNEIKN YWRTHFKKKAKPTTNNAEKIKSRLLKRQHFKEQREIELQQEQQLFQFDQLGMKKIISLLEENNSSSSSDGGGDVFYYPDQITHSPKPFGYNSNSLEEQLQ GRFSPVNIPDANTMNEDNAIWDGFWNMDVVNGHGGNLGVVAATAACGPRKPYFHNLVIPFC
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 782/783 (99.9%)（アミノ酸配列で260/261 (99.6%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G13 ATGGTGGAAGAAGTTTGGAGAAAGGGTCCATGGACCGCCGAAGAAGACCGTCTTTTGATCGAATACGTCCGTGTTCACGGTGAAGGTCGTTGGAACTCTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02C ATGGTGGAAGAAGTTTGGAGAAAGGGTCCATGGACCGCCGAAGAAGACCGTCTTTTGATCGAATACGTCCGTGTTCACGGTGAAGGTCGTTGGAACTCTG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G13 TCTCTAAACTCGCAGGATTGAAAAGGAATGGCAAAAGCTGCAGACTAAGATGGGTGAATTACCTTAGACCAGACCTCAAGAGAGGACAGATCACTCCACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02C TCTCTAAACTCGCAGGATTGAAAAGGAATGGCAAAAGCTGCAGACTAAGATGGGTGAATTACCTTAGACCAGACCTCAAGAGAGGACAGATCACTCCACA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G13 TGAAGAAAGTATAATACTTGAGCTACACGCTAAGTGGGGAAATAGGTGGTCAACAATTGCACGTAGTTTACCAGGAAGAACAGACAATGAGATCAAGAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02C TGAAGAAAGTATAATACTTGAGCTACACGCTAAGTGGGGAAATAGGTGGTCAACAATTGCACGTAGTTTACCAGGAAGAACAGACAATGAGATCAAGAAC 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT4G13 TATTGGAGAACCCATTTCAAGAAAAAGGCAAAGCCTACGACTAACAATGCGGAGAAGATAAAGAGTCGTCTCCTAAAAAGGCAACACTTCAAGGAACAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02C TATTGGAGAACCCATTTCAAGAAAAAGGCAAAGCCTACGACTAACAATGCGGAGAAGATAAAGAGTCGTCTCCTAAAAAGGCAACACTTCAAGGAACAGA 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT4G13 GAGAAATAGAGTTGCAACAAGAACAGCAGTTGTTTCAGTTCGACCAACTCGGTATGAAAAAGATCATCTCTTTACTCGAAGAAAACAATAGCAGTAGCAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02C GAGAAATAGAGTTGCAACAAGAACAGCAGTTGTTTCAGTTCGACCAACTCGGTATGAAAAAGATCATCTCTTTACTCGAAGAAAACAATAGCAGTAGCAG 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT4G13 TAGCGATGGCGGTGGTGATGTGTTCTATTATCCTGATCAAATAACACATTCATCAAAACCCTTTGGCTATAACTCTAATTCATTAGAGGAGCAGTTACAA :::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::: IE_02C TAGCGATGGCGGTGGTGATGTGTTCTATTATCCTGATCAAATAACACATTCACCAAAACCCTTTGGCTATAACTCTAATTCATTAGAGGAGCAGTTACAA 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT4G13 GGTAGATTTTCTCCTGTAAACATACCTGATGCTAATACTATGAACGAAGACAATGCCATATGGGACGGGTTTTGGAACATGGATGTTGTAAATGGACATG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02C GGTAGATTTTCTCCTGTAAACATACCTGATGCTAATACTATGAACGAAGACAATGCCATATGGGACGGGTTTTGGAACATGGATGTTGTAAATGGACATG 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 AT4G13 GTGGGAACTTGGGTGTTGTGGCTGCTACTGCTGCTTGTGGCCCAAGGAAGCCCTATTTCCATAACTTGGTGATTCCATTTTGT ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02C GTGGGAACTTGGGTGTTGTGGCTGCTACTGCTGCTTGTGGCCCAAGGAAGCCCTATTTCCATAACTTGGTGATTCCATTTTGT 710 720 730 740 750 760 770 780 10 20 30 40 50 60 70 80 90 100 AT4G13 MVEEVWRKGPWTAEEDRLLIEYVRVHGEGRWNSVSKLAGLKRNGKSCRLRWVNYLRPDLKRGQITPHEESIILELHAKWGNRWSTIARSLPGRTDNEIKN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02C MVEEVWRKGPWTAEEDRLLIEYVRVHGEGRWNSVSKLAGLKRNGKSCRLRWVNYLRPDLKRGQITPHEESIILELHAKWGNRWSTIARSLPGRTDNEIKN 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G13 YWRTHFKKKAKPTTNNAEKIKSRLLKRQHFKEQREIELQQEQQLFQFDQLGMKKIISLLEENNSSSSSDGGGDVFYYPDQITHSSKPFGYNSNSLEEQLQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::: IE_02C YWRTHFKKKAKPTTNNAEKIKSRLLKRQHFKEQREIELQQEQQLFQFDQLGMKKIISLLEENNSSSSSDGGGDVFYYPDQITHSPKPFGYNSNSLEEQLQ 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 AT4G13 GRFSPVNIPDANTMNEDNAIWDGFWNMDVVNGHGGNLGVVAATAACGPRKPYFHNLVIPFC ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02C GRFSPVNIPDANTMNEDNAIWDGFWNMDVVNGHGGNLGVVAATAACGPRKPYFHNLVIPFC 210 220 230 240 250 260

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