| Code name | IE_02C08 | |||
|---|---|---|---|---|
| Locus | AT1G43950 | |||
| Template | IT_02C08 (HR2223) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_02C08 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAAGTGGCAATGTTGTGAATGTAC) | |||
| Reverse primer | IPR_02C08 (ggggaccactttgtacaagaaagctgggttTCTGATACCAACTCGTAACTCTCCAGTCTC) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列88.00%一致 | - | ||
| comment | 1st:Templateの配列とは一致 | |||
| sequence |
>IE_02C08_675bp ATGGAAAGTGGCAATGTTGTGAATGTACAACCTGAATTATCAGGCATAATTGATGGATCCAAGAGCTATATGTACGAGCAGTTATGGAAACTCTGTGCGG GACCTTTGTGTGATATTCCAAAACTTGGAGAAAATGTTTATTACTTTCCTCAAGGCAATATAGAGCTTGTTGATGCATCTACAAGAGAAGAGTTGAATGA GTTACAACCAATTTGTGATCTTCCTTCTAAGCTTCAATGCCGTGTTATTGCAATTCATCTTAAGGTGGAAAACAATAGTGATGAGACTTATGCTGAGATC ACTTTGATGCCAGATACAACTCAAGTTGTAATCCCTACTCAGAGTGAAAATCAATTTAGGCCATTAGTTAATTCCTTTACGAAGGTTTTAACGGCCTCTG ATACAAGCGCTTATGGTGGATTCTTTGTTCCGAAAAAACATGCCATTGAATGTCTCCCTCCACTGGATATGTCTCAGCCTCTACCGGCACAAGAACTTCT TGCTAAAGATCTCCATGGTAATCAATGGAGATTCAGACACAGCTATAGAGGTACACCGCAAAGACATTCGTTAACAACTGGTTGGAATGAATTCACAACA TCGAAAAAGTTGGTTAAAGGAGATGTTATTGTATTCGTTAGGGGAGAGACTGGAGAGTTACGAGTTGGTATCAGA >IE_02C08_translated MESGNVVNVQPELSGIIDGSKSYMYEQLWKLCAGPLCDIPKLGENVYYFPQGNIELVDASTREELNELQPICDLPSKLQCRVIAIHLKVENNSDETYAEI TLMPDTTQVVIPTQSENQFRPLVNSFTKVLTASDTSAYGGFFVPKKHAIECLPPLDMSQPLPAQELLAKDLHGNQWRFRHSYRGTPQRHSLTTGWNEFTT SKKLVKGDVIVFVRGETGELRVGIR |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 622/675 (92.1%)（アミノ酸配列で198/225 (88.0%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT1G43 ATGGAAAGTGGCAATGTTGTGAATGTACAGTCTGAATTATCTGGCATAATTGATGGGTCCAAGAGCTATATGTACGAGCAGTTATGGAAACTCTGTGCGG
::::::::::::::::::::::::::::: :::::::::: :::::::::::::: :::::::::::::::::::::::::::::::::::::::::::
IE_02C ATGGAAAGTGGCAATGTTGTGAATGTACAACCTGAATTATCAGGCATAATTGATGGATCCAAGAGCTATATGTACGAGCAGTTATGGAAACTCTGTGCGG
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT1G43 GACCTTTGTGTGATATTCCAAAACTTGGAGAAAAAGTGTATTACTTTCCTCAAGGCCATATAGAGCTTGTTGAGGCATCTACTAGAGAAGAGTTGAATGA
:::::::::::::::::::::::::::::::::: :: :::::::::::::::::: :::::::::::::::: :::::::: :::::::::::::::::
IE_02C GACCTTTGTGTGATATTCCAAAACTTGGAGAAAATGTTTATTACTTTCCTCAAGGCAATATAGAGCTTGTTGATGCATCTACAAGAGAAGAGTTGAATGA
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270 280 290 300
AT1G43 GTTACAACCAAATTGTGATCTTCCTTCTAAGCTTCAATGTCGTGTTATTGCTATTCATCTTAAGGTGGAAAACAATAGTGATGAGACTTATGTTGAGATT
::::::::::: ::::::::::::::::::::::::::: ::::::::::: :::::::::::::::::::::::::::::::::::::::: ::::::
IE_02C GTTACAACCAATTTGTGATCTTCCTTCTAAGCTTCAATGCCGTGTTATTGCAATTCATCTTAAGGTGGAAAACAATAGTGATGAGACTTATGCTGAGATC
210 220 230 240 250 260 270 280 290 300
310 320 330 340 350 360 370 380 390 400
AT1G43 ACTTTGATGCCAGATACAACTCAAGTTGTAATCCCTACTGAGAATGAAAATCAATTTAGGCCAATAGTTAATTCCTTTACAAAGGTTTTAACGGCTTCAG
::::::::::::::::::::::::::::::::::::::: ::: ::::::::::::::::::: :::::::::::::::: :::::::::::::: :: :
IE_02C ACTTTGATGCCAGATACAACTCAAGTTGTAATCCCTACTCAGAGTGAAAATCAATTTAGGCCATTAGTTAATTCCTTTACGAAGGTTTTAACGGCCTCTG
310 320 330 340 350 360 370 380 390 400
410 420 430 440 450 460 470 480 490 500
AT1G43 ATACAAGCGCTCAAGGTGAATTCTCTGTTCCATGTAAACATGCCATTGAATGTCTCCCTCCGCTGGATATGTCTCAACCTATACCGGCACAAGAACTTAT
::::::::::: : :::: ::::: :::::: :::::::::::::::::::::::::: :::::::::::::: ::: ::::::::::::::::: :
IE_02C ATACAAGCGCTTATGGTGGATTCTTTGTTCCGAAAAAACATGCCATTGAATGTCTCCCTCCACTGGATATGTCTCAGCCTCTACCGGCACAAGAACTTCT
410 420 430 440 450 460 470 480 490 500
510 520 530 540 550 560 570 580 590
AT1G43 TGCTATAGATCTCCATGGTAATCAATGGAGATTCAAACACAGCTATAGA-GTACCCCGCGGAGAC--------ACAACTGGTTGGAATGCATTCACAACA
::::: ::::::::::::::::::::::::::::: ::::::::::::: :::: :::: :::: :::::::::::::::: ::::::::::
IE_02C TGCTAAAGATCTCCATGGTAATCAATGGAGATTCAGACACAGCTATAGAGGTACACCGCAAAGACATTCGTTAACAACTGGTTGGAATGAATTCACAACA
510 520 530 540 550 560 570 580 590 600
600 610 620 630 640 650 660
AT1G43 TCAAAAAAATTGGTTGTAGGGGATGTTATTGTATTCGCTAGGGGAGAGACTGGAGAGTTACGAGTTGGTATCAGA
:: ::::: :::::: ::: :::::::::::::::: :::::::::::::::::::::::::::::::::::::
IE_02C TCGAAAAAGTTGGTTAAAGGAGATGTTATTGTATTCGTTAGGGGAGAGACTGGAGAGTTACGAGTTGGTATCAGA
610 620 630 640 650 660 670
10 20 30 40 50 60 70 80 90 100
AT1G43 MESGNVVNVQSELSGIIDGSKSYMYEQLWKLCAGPLCDIPKLGEKVYYFPQGHIELVEASTREELNELQPNCDLPSKLQCRVIAIHLKVENNSDETYVEI
:::::::::: ::::::::::::::::::::::::::::::::: :::::::.::::.:::::::::::: :::::::::::::::::::::::::: ::
IE_02C MESGNVVNVQPELSGIIDGSKSYMYEQLWKLCAGPLCDIPKLGENVYYFPQGNIELVDASTREELNELQPICDLPSKLQCRVIAIHLKVENNSDETYAEI
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190
AT1G43 TLMPDTTQVVIPTENENQFRPIVNSFTKVLTASDTSAQGEFSVPCKHAIECLPPLDMSQPIPAQELIAIDLHGNQWRFKHSYR-VPRGD--TTGWNAFTT
:::::::::::::..::::::.::::::::::::::: : : :: :::::::::::::::.:::::.: :::::::::.:::: :. ::::: :::
IE_02C TLMPDTTQVVIPTQSENQFRPLVNSFTKVLTASDTSAYGGFFVPKKHAIECLPPLDMSQPLPAQELLAKDLHGNQWRFRHSYRGTPQRHSLTTGWNEFTT
110 120 130 140 150 160 170 180 190 200
200 210 220
AT1G43 SKKLVVGDVIVFARGETGELRVGIR
::::: :::::: ::::::::::::
IE_02C SKKLVKGDVIVFVRGETGELRVGIR
210 220
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