◆◆◆IE_02D05（IE_09A02） detail◆◆◆

Code nameIE_02D05（IE_09A02）
LocusAT5G04150
TemplateIT_02D05 (HR2378)
Donor VectorpDONR207
Forward primerIPF_02D05 (ggggacaagtttgtacaaaaaagcaggcttcATGGAGTATCCATGGCTGCAGTCTCAAG)
Reverse primerIPR_02D05 (ggggaccactttgtacaagaaagctgggttTGATTGGCGTAATCCCAAGAGCATACCATT)
Alignment with template seq.1st配列解析が行えなかったサンプルです。2ndアミノ酸配列100%一致
Alignment with TAIR7CDS-アミノ酸配列100%一致
comment1st:(PCRで目的サイズの増幅が確認できたが、コロニーが得られなかった)→2nd(09A02):100%
sequence 
>IE_02D05_720bp
ATGGAGTATCCATGGCTGCAGTCTCAAGTTCATTCCTTTTCACCTACTCTCCATTTTCCTTCCTTCCTTCATCCTTTAGATGATTCCAAGAGCCATAACA
TCAATCTTCATCATATGAGTCTTAGTCACAGCAATAATACTAACAGTAACAATAACAATTATCAAGAAGAAGATCGAGGAGCGGTGGTTTTGGAGAAGAA
ACTGAATCACAACGCAAGCGAACGAGACCGCCGTAGAAAACTTAACGCCTTGTACTCTTCACTTCGTGCTCTCTTGCCTCTTTCTGATCAAAAGAGGAAG
CTGAGCATTCCTATGACGGTAGCGAGAGTAGTGAAATACATACCAGAGCAGAAGCAAGAACTTCAACGTTTGTCTCGGAGAAAAGAAGAGCTCTTGAAGA
GGATCTCGAGAAAAACTCACCAAGAGCAGCTGAGAAACAAAGCAATGATGGACTCAATAGATTCTTCTTCCTCTCAACGGATCGCAGCAAATTGGCTCAC
TGACACAGAGATTGCTGTCCAGATTGCTACGTCGAAATGGACATCTGTTTCAGACATGTTGCTTAGGTTAGAAGAAAACGGGCTTAATGTCATAAGCGTC
TCTTCTTCCGTTTCTTCCACCGCAAGGATCTTCTACACTCTACATCTTCAGATGAGAGGAGATTGCAAAGTGAGACTGGAGGAACTCATCAATGGTATGC
TCTTGGGATTACGCCAATCA

>IE_02D05_translated
MEYPWLQSQVHSFSPTLHFPSFLHPLDDSKSHNINLHHMSLSHSNNTNSNNNNYQEEDRGAVVLEKKLNHNASERDRRRKLNALYSSLRALLPLSDQKRK
LSIPMTVARVVKYIPEQKQELQRLSRRKEELLKRISRKTHQEQLRNKAMMDSIDSSSSQRIAANWLTDTEIAVQIATSKWTSVSDMLLRLEENGLNVISV
SSSVSSTARIFYTLHLQMRGDCKVRLEELINGMLLGLRQS
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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