◆◆◆IE_02D07 detail◆◆◆

Code name	IE_02D07
Locus	AT2G41690
Template	IT_02D07 (HR2419)
Donor Vector	pDONR207
Forward primer	IPF_02D07 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAGATGCTGGTGAACATTTACGGT)
Reverse primer	IPR_02D07 (ggggaccactttgtacaagaaagctgggttTTCAAGTTTTACTCCGAACAACTTAAGCCC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.20%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_02D07_732bp ATGGAAGATGCTGGTGAACATTTACGGTGTAACGATAACGTTAACGACGAGGAGCGTTTGCCATTGGAGTTTATGATCGGAAAGTCAACATCCACGGCGG AGCTACAGCCGCCTCCACCGTTCTTGGTAAAGACATACAAAGTGGTGGAGGATCCGACGACGGACGGGGTTATATCTTGGAACGAATACGGAACTGGCTT CGTCGTGTGGCAGCCGGCAGAATTCGCTAGAGATCTGTTACCAACACTTTTCAAGCATTGCAACTTCTCTAGCTTCGTTCGCCAGCTCAATACTTACGGT TTTCGAAAAGTAACGACGATAAGATGGGAATTTAGTAATGAGATGTCTCGAAAGGGGCAAAGAGAGCTTATGAGCAATATCCGAAGAAGGAAGAGCCAAC ATTGGTCACACAACAAGTCTAATCACCAGGTTGTACCAACAACAACGATGGTGAATCAAGAAGGTCATCAACGGATTGGGATTGATCATCACCATGAGGA TCAACAGTCTTCCGCCACTTCATCCTCTTTTGTATACACTGCATTACTCGACGAAAACAAATGCTTGAAGAATGAAAACGAGTTATTAAGCTGCGAACTT GGGAAAACCAAGAAGAAATGCAAGCAGCTTATGGAGTTGGTGGAGAGATACAGAGGAGAAGACGAAGATGCAACTGATGAAAGTGATGATGAAGAAGATG AAGGGCTTAAGTTGTTCGGAGTAAAACTTGAA >IE_02D07_translated MEDAGEHLRCNDNVNDEERLPLEFMIGKSTSTAELQPPPPFLVKTYKVVEDPTTDGVISWNEYGTGFVVWQPAEFARDLLPTLFKHCNFSSFVRQLNTYG FRKVTTIRWEFSNEMSRKGQRELMSNIRRRKSQHWSHNKSNHQVVPTTTMVNQEGHQRIGIDHHHEDQQSSATSSSFVYTALLDENKCLKNENELLSCEL GKTKKKCKQLMELVERYRGEDEDATDESDDEEDEGLKLFGVKLE
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 728/732 (99.5%)（アミノ酸配列で242/244 (99.2%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT2G41 ATGGAAGATGCTGGTGAACATTTACGGTGTAACGATAACGTTAACGACGAGGAGCGTTTGCCATTGGAGTTTATGATCGGAAACTCAACATCCACGGCGG ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::: IE_02D ATGGAAGATGCTGGTGAACATTTACGGTGTAACGATAACGTTAACGACGAGGAGCGTTTGCCATTGGAGTTTATGATCGGAAAGTCAACATCCACGGCGG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT2G41 AGCTACAGCCGCCTCCACCGTTCTTGGTAAAGACATACAAAGTGGTGGAGGATCCGACGACGGACGGGGTTATATCTTGGAACGAATACGGAACTGGTTT ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :: IE_02D AGCTACAGCCGCCTCCACCGTTCTTGGTAAAGACATACAAAGTGGTGGAGGATCCGACGACGGACGGGGTTATATCTTGGAACGAATACGGAACTGGCTT 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT2G41 CGTCGTGTGGCAGCCGGCAGAATTCGCTAGAGATCTGTTACCAACACTTTTCAAGCATTGCAACTTCTCTAGCTTCGTTCGCCAGCTCAATACTTACGGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02D CGTCGTGTGGCAGCCGGCAGAATTCGCTAGAGATCTGTTACCAACACTTTTCAAGCATTGCAACTTCTCTAGCTTCGTTCGCCAGCTCAATACTTACGGT 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT2G41 TTTCGAAAAGTAACGACGATAAGATGGGAATTTAGTAATGAGATGTTTCGAAAGGGGCAAAGAGAGCTTATGAGCAATATCCGAAGAAGGAAGAGCCAAC :::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02D TTTCGAAAAGTAACGACGATAAGATGGGAATTTAGTAATGAGATGTCTCGAAAGGGGCAAAGAGAGCTTATGAGCAATATCCGAAGAAGGAAGAGCCAAC 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT2G41 ATTGGTCACACAACAAGTCTAATCACCAGGTTGTACCAACAACAACGATGGTGAATCAAGAAGGTCATCAACGGATTGGGATTGATCATCACCATGAGGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02D ATTGGTCACACAACAAGTCTAATCACCAGGTTGTACCAACAACAACGATGGTGAATCAAGAAGGTCATCAACGGATTGGGATTGATCATCACCATGAGGA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT2G41 TCAACAGTCTTCCGCCACTTCATCCTCTTTCGTATACACTGCATTACTCGACGAAAACAAATGCTTGAAGAATGAAAACGAGTTATTAAGCTGCGAACTT :::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02D TCAACAGTCTTCCGCCACTTCATCCTCTTTTGTATACACTGCATTACTCGACGAAAACAAATGCTTGAAGAATGAAAACGAGTTATTAAGCTGCGAACTT 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT2G41 GGGAAAACCAAGAAGAAATGCAAGCAGCTTATGGAGTTGGTGGAGAGATACAGAGGAGAAGACGAAGATGCAACTGATGAAAGTGATGATGAAGAAGATG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02D GGGAAAACCAAGAAGAAATGCAAGCAGCTTATGGAGTTGGTGGAGAGATACAGAGGAGAAGACGAAGATGCAACTGATGAAAGTGATGATGAAGAAGATG 610 620 630 640 650 660 670 680 690 700 710 720 730 AT2G41 AAGGGCTTAAGTTGTTCGGAGTAAAACTTGAA :::::::::::::::::::::::::::::::: IE_02D AAGGGCTTAAGTTGTTCGGAGTAAAACTTGAA 710 720 730 10 20 30 40 50 60 70 80 90 100 AT2G41 MEDAGEHLRCNDNVNDEERLPLEFMIGNSTSTAELQPPPPFLVKTYKVVEDPTTDGVISWNEYGTGFVVWQPAEFARDLLPTLFKHCNFSSFVRQLNTYG ::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02D MEDAGEHLRCNDNVNDEERLPLEFMIGKSTSTAELQPPPPFLVKTYKVVEDPTTDGVISWNEYGTGFVVWQPAEFARDLLPTLFKHCNFSSFVRQLNTYG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT2G41 FRKVTTIRWEFSNEMFRKGQRELMSNIRRRKSQHWSHNKSNHQVVPTTTMVNQEGHQRIGIDHHHEDQQSSATSSSFVYTALLDENKCLKNENELLSCEL ::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02D FRKVTTIRWEFSNEMSRKGQRELMSNIRRRKSQHWSHNKSNHQVVPTTTMVNQEGHQRIGIDHHHEDQQSSATSSSFVYTALLDENKCLKNENELLSCEL 110 120 130 140 150 160 170 180 190 200 210 220 230 240 AT2G41 GKTKKKCKQLMELVERYRGEDEDATDESDDEEDEGLKLFGVKLE :::::::::::::::::::::::::::::::::::::::::::: IE_02D GKTKKKCKQLMELVERYRGEDEDATDESDDEEDEGLKLFGVKLE 210 220 230 240

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