| Code name | IE_02D09 | |||
|---|---|---|---|---|
| Locus | AT4G25410 | |||
| Template | IT_02D09 (HR2506) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_02D09 (ggggacaagtttgtacaaaaaagcaggcttcATGGATCCTTATAAGAATCTTAATCCAA) | |||
| Reverse primer | IPR_02D09 (ggggaccactttgtacaagaaagctgggttGTACCCCGTCGAAAGTATCAAATCCTCAAC) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列100%一致 | - | ||
| comment | ||||
| sequence |
>IE_02D09_663bp ATGGATCCTTATAAGAATCTTAATCCAAAAGGTTACCAGAGACAGAGACCGTTTAGCTCAGCCGGCGAGAGTGGCGGCAGCGGCGGCTCCGGTACGGCCC ATGAAACAGATGACAATAAGAAGAAGAAGAAGCTTCTCCACCGCGACATCGAACGCCAAAGAAGACAAGAAATGGCTACACTCTTTGCTACTCTTCGTAC TCACTTACCTCTTAAATACATCAAGGGAAAAAGAGCTGTGTCGGATCATGTAAATGGAGCGGTAAATTTCATTAAGGACACGGAAGCACGGATTAAAGAA CTTAGTGCAAGAAGAGACGAGTTAAGTAGAGAAACCGGCCAAGGATATAAATCGAATCCGGATCCAGGAAAAACTGGATCCGATGTAGGCAAATCGGAGC CGGCGACTGTGATGGTGCAACCACACGTGAGCGGTTTAGAAGTGGTAGTGAGCAGCAACTCCTCAGGCCCCGAAGCTTTGCCACTATCAAAAGTGCTCGA GACAATTCAGGAGAAAGGGCTTGAAGTCATGAGCTCCTTCACTACAAGAGTCAATGATAGGCTCATGCACACTATTCAAGTAGAGGTTAATAGTTTCGGA TGCATAGACTTATTATGGTTGCAGCAGAAGCTAGTTGAGGATTTGATACTTTCGACGGGGTAC >IE_02D09_translated MDPYKNLNPKGYQRQRPFSSAGESGGSGGSGTAHETDDNKKKKKLLHRDIERQRRQEMATLFATLRTHLPLKYIKGKRAVSDHVNGAVNFIKDTEARIKE LSARRDELSRETGQGYKSNPDPGKTGSDVGKSEPATVMVQPHVSGLEVVVSSNSSGPEALPLSKVLETIQEKGLEVMSSFTTRVNDRLMHTIQVEVNSFG CIDLLWLQQKLVEDLILSTGY |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 660/690 (95.7%)（アミノ酸配列で220/230 (95.7%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT4G25 ATGGATCCTTATAAGAATCTTAATCCAAAAGGTTACCAGAGACAGAGACCGTTTAGCTCAGCCGGCGAGAGTGGCGGCAGCGGCGGCTCCGGTACGGCCC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02D ATGGATCCTTATAAGAATCTTAATCCAAAAGGTTACCAGAGACAGAGACCGTTTAGCTCAGCCGGCGAGAGTGGCGGCAGCGGCGGCTCCGGTACGGCCC
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT4G25 ATGAAACAGATGACAATAAGAAGAAGAAGAAGCTTCTCCACCGCGACATCGAACGCCAAAGAAGACAAGAAATGGCTACACTCTTTGCTACTCTTCGTAC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02D ATGAAACAGATGACAATAAGAAGAAGAAGAAGCTTCTCCACCGCGACATCGAACGCCAAAGAAGACAAGAAATGGCTACACTCTTTGCTACTCTTCGTAC
110 120 130 140 150 160 170 180 190 200
210 220 230 240 250 260 270 280 290 300
AT4G25 TCACTTACCTCTTAAATACATCAAGGGAAAAAGAGCTGTGTCGGATCATGTAAATGGAGCGGTAAATTTCATTAAGGACACGGAAGCACGGATTAAAGAA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02D TCACTTACCTCTTAAATACATCAAGGGAAAAAGAGCTGTGTCGGATCATGTAAATGGAGCGGTAAATTTCATTAAGGACACGGAAGCACGGATTAAAGAA
210 220 230 240 250 260 270 280 290 300
310 320 330 340 350 360 370 380 390 400
AT4G25 CTTAGTGCAAGAAGAGACGAGTTAAGTAGAGAAACCGGCCAAGGATATAAATCGAATCCGGATCCAGGAAAAACTGGATCCGATGTAGGCAAATCGGAGC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02D CTTAGTGCAAGAAGAGACGAGTTAAGTAGAGAAACCGGCCAAGGATATAAATCGAATCCGGATCCAGGAAAAACTGGATCCGATGTAGGCAAATCGGAGC
310 320 330 340 350 360 370 380 390 400
410 420 430 440 450 460 470 480 490 500
AT4G25 CGGCGACTGTGATGGTGCAACCACACGTGAGCGGTTTAGAAGTGGTAGTGAGCAGCAACTCCTCAGGCCCCGAAGCTTTGCCACTATCAAAAGTGCTCGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02D CGGCGACTGTGATGGTGCAACCACACGTGAGCGGTTTAGAAGTGGTAGTGAGCAGCAACTCCTCAGGCCCCGAAGCTTTGCCACTATCAAAAGTGCTCGA
410 420 430 440 450 460 470 480 490 500
510 520 530 540 550 560 570 580 590 600
AT4G25 GACAATTCAGGAGAAAGGGCTTGAAGTCATGAGCTCCTTCACTACAAGAGTCAATGATAGGCTCATGCACACTATTCAAGTAGAGGTTAATAGTTTCGGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02D GACAATTCAGGAGAAAGGGCTTGAAGTCATGAGCTCCTTCACTACAAGAGTCAATGATAGGCTCATGCACACTATTCAAGTAGAGGTTAATAGTTTCGGA
510 520 530 540 550 560 570 580 590 600
610 620 630 640 650 660 670 680 690
AT4G25 TGCATAGACTTATTATGGTTGCAGCAGAAGCTAGTTGAGGATTTGATACTTTCGACGGGAAAAAATCAAAGTCTTCTTGAAATTTCGTTA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :
IE_02D TGCATAGACTTATTATGGTTGCAGCAGAAGCTAGTTGAGGATTTGATACTTTCGACGGGGTAC---------------------------
610 620 630 640 650 660
10 20 30 40 50 60 70 80 90 100
AT4G25 MDPYKNLNPKGYQRQRPFSSAGESGGSGGSGTAHETDDNKKKKKLLHRDIERQRRQEMATLFATLRTHLPLKYIKGKRAVSDHVNGAVNFIKDTEARIKE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02D MDPYKNLNPKGYQRQRPFSSAGESGGSGGSGTAHETDDNKKKKKLLHRDIERQRRQEMATLFATLRTHLPLKYIKGKRAVSDHVNGAVNFIKDTEARIKE
10 20 30 40 50 60 70 80 90 100
110 120 130 140 150 160 170 180 190 200
AT4G25 LSARRDELSRETGQGYKSNPDPGKTGSDVGKSEPATVMVQPHVSGLEVVVSSNSSGPEALPLSKVLETIQEKGLEVMSSFTTRVNDRLMHTIQVEVNSFG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02D LSARRDELSRETGQGYKSNPDPGKTGSDVGKSEPATVMVQPHVSGLEVVVSSNSSGPEALPLSKVLETIQEKGLEVMSSFTTRVNDRLMHTIQVEVNSFG
110 120 130 140 150 160 170 180 190 200
210 220 230
AT4G25 CIDLLWLQQKLVEDLILSTGKNQSLLEISL
::::::::::::::::::::
IE_02D CIDLLWLQQKLVEDLILSTGY---------
210 220
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