◆◆◆IE_02F02 detail◆◆◆

Code name	IE_02F02
Locus	AT5G62320
Template	IT_02F02 (HR2720)
Donor Vector	pDONR207
Forward primer	IPF_02F02 (ggggacaagtttgtacaaaaaagcaggcttcATGGGTGGTCGTAAACCATGTTGTGATG)
Reverse primer	IPR_02F02 (ggggaccactttgtacaagaaagctgggttAACATCGAAACATCCAAGTTCTAAATCTTC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.60%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_02F02_735bp ATGGGTGGTCGTAAACCATGTTGTGATGAGGTTGGATTAAGAAAGGGTCCATGGACAGTGGAAGAAGATGGGAAACTAGTTGATTTCTTAAGGGCACGTG GCAACTGCGGTGGTGGTGGAGGAGGATGGTGCTGGAGAGACGTGCCAAAACTGGCGGGGCTAAGGAGGTGTGGCAAAAGTTGCCGTCTCCGGTGGACTAA TTATCTCCGGCCAGATCTCAAGAGAGGTCTTTTTACTGAAGAAGAAATCCAACTAGTCATTGATCTTCATGCTCGCCTTGGCAATAGATGGTCGAAGATT GCAGTGGAGTTACCAGGAAGAACAGACAACGATATCAAAAATTATTGGAACACTCATATAAAGAGGAAGCTTATAAGAATGGGTATTGATCCAAACACAC ATCGTCGATTTGACCAACAAAAAGTCAACGAGGAGGAAACGATATTGGTCAACGATCCAAAGCCTCTGTCTGAGACCGAGGCATCTGTTGCTTTGAAGAA TGACACGTCAGCAGTGTTATCAGGAAATCTAAACCAATTGGCTGACGTGGACGGTGATGATCAGCCGTGGAGCTTTCTAATGGAAAATGACGAAGGAGGA GGTGGCGACGCCGCCGGAGAGCTTACGATGCTATTGTCCGGTGACATTACGTCATCATGTTCTTCTTCGTCATCTTTGTGGATGAAGTATGGAGAATTCG GATACGAAGATTTAGAACTTGGATGTTTCGATGTT >IE_02F02_translated MGGRKPCCDEVGLRKGPWTVEEDGKLVDFLRARGNCGGGGGGWCWRDVPKLAGLRRCGKSCRLRWTNYLRPDLKRGLFTEEEIQLVIDLHARLGNRWSKI AVELPGRTDNDIKNYWNTHIKRKLIRMGIDPNTHRRFDQQKVNEEETILVNDPKPLSETEASVALKNDTSAVLSGNLNQLADVDGDDQPWSFLMENDEGG GGDAAGELTMLLSGDITSSCSSSSSLWMKYGEFGYEDLELGCFDV
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 734/735 (99.9%)（アミノ酸配列で244/245 (99.6%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G62 ATGGGTGGTCGTAAACCATGTTGTGATGAGGTTGGATTAAGAAAGGGTCCATGGACAGTGGAAGAAGATGGGAAACTAGTTGATTTCTTAAGGGCACGTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02F ATGGGTGGTCGTAAACCATGTTGTGATGAGGTTGGATTAAGAAAGGGTCCATGGACAGTGGAAGAAGATGGGAAACTAGTTGATTTCTTAAGGGCACGTG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G62 GCAACTGCGGTGGTGGTGGAGGAGGATGGTGCTGGAGAGACGTGCCAAAACTGGCGGGGCTAAGGAGGTGTGGCAAAAGTTGCCGTCTCCGGTGGACTAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02F GCAACTGCGGTGGTGGTGGAGGAGGATGGTGCTGGAGAGACGTGCCAAAACTGGCGGGGCTAAGGAGGTGTGGCAAAAGTTGCCGTCTCCGGTGGACTAA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G62 TTATCTCCGGCCAGATCTCAAGAGAGGTCTTTTTACTGAAGAAGAAATCCAACTAGTCATTGATCTTCATGCTCGCCTTGGCAATAGATGGTCGAAGATT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02F TTATCTCCGGCCAGATCTCAAGAGAGGTCTTTTTACTGAAGAAGAAATCCAACTAGTCATTGATCTTCATGCTCGCCTTGGCAATAGATGGTCGAAGATT 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G62 GCAGTGGAGTTACCAGGAAGAACAGACAACGATATCAAAAATTATTGGAACACTCATATAAAGAGGAAGCTTATAAGAATGGGTATTGATCCAAACACAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02F GCAGTGGAGTTACCAGGAAGAACAGACAACGATATCAAAAATTATTGGAACACTCATATAAAGAGGAAGCTTATAAGAATGGGTATTGATCCAAACACAC 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G62 ATCGTCGATTTGACCAACAAAAAGTCAACGAGGAGGAAACGATATTGGTCAACGATCCAAAGCCTCTGTCTGAGACCGAGGTATCTGTTGCTTTGAAGAA ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::: IE_02F ATCGTCGATTTGACCAACAAAAAGTCAACGAGGAGGAAACGATATTGGTCAACGATCCAAAGCCTCTGTCTGAGACCGAGGCATCTGTTGCTTTGAAGAA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G62 TGACACGTCAGCAGTGTTATCAGGAAATCTAAACCAATTGGCTGACGTGGACGGTGATGATCAGCCGTGGAGCTTTCTAATGGAAAATGACGAAGGAGGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02F TGACACGTCAGCAGTGTTATCAGGAAATCTAAACCAATTGGCTGACGTGGACGGTGATGATCAGCCGTGGAGCTTTCTAATGGAAAATGACGAAGGAGGA 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G62 GGTGGCGACGCCGCCGGAGAGCTTACGATGCTATTGTCCGGTGACATTACGTCATCATGTTCTTCTTCGTCATCTTTGTGGATGAAGTATGGAGAATTCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02F GGTGGCGACGCCGCCGGAGAGCTTACGATGCTATTGTCCGGTGACATTACGTCATCATGTTCTTCTTCGTCATCTTTGTGGATGAAGTATGGAGAATTCG 610 620 630 640 650 660 670 680 690 700 710 720 730 AT5G62 GATACGAAGATTTAGAACTTGGATGTTTCGATGTT ::::::::::::::::::::::::::::::::::: IE_02F GATACGAAGATTTAGAACTTGGATGTTTCGATGTT 710 720 730 10 20 30 40 50 60 70 80 90 100 AT5G62 MGGRKPCCDEVGLRKGPWTVEEDGKLVDFLRARGNCGGGGGGWCWRDVPKLAGLRRCGKSCRLRWTNYLRPDLKRGLFTEEEIQLVIDLHARLGNRWSKI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_02F MGGRKPCCDEVGLRKGPWTVEEDGKLVDFLRARGNCGGGGGGWCWRDVPKLAGLRRCGKSCRLRWTNYLRPDLKRGLFTEEEIQLVIDLHARLGNRWSKI 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G62 AVELPGRTDNDIKNYWNTHIKRKLIRMGIDPNTHRRFDQQKVNEEETILVNDPKPLSETEVSVALKNDTSAVLSGNLNQLADVDGDDQPWSFLMENDEGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::: IE_02F AVELPGRTDNDIKNYWNTHIKRKLIRMGIDPNTHRRFDQQKVNEEETILVNDPKPLSETEASVALKNDTSAVLSGNLNQLADVDGDDQPWSFLMENDEGG 110 120 130 140 150 160 170 180 190 200 210 220 230 240 AT5G62 GGDAAGELTMLLSGDITSSCSSSSSLWMKYGEFGYEDLELGCFDV ::::::::::::::::::::::::::::::::::::::::::::: IE_02F GGDAAGELTMLLSGDITSSCSSSSSLWMKYGEFGYEDLELGCFDV 210 220 230 240

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