| Code name | IE_02F03ï¼IE_09A03ï¼ | |||
|---|---|---|---|---|
| Locus | AT2G25820 | |||
| Template | IT_02F03 (HR2726) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_02F03 (ggggacaagtttgtacaaaaaagcaggcttcATGGCAGCGCGTGCCCATGATGTTGCGG) | |||
| Reverse primer | IPR_02F03 (ggggaccactttgtacaagaaagctgggttCTCATAATTCCACAAAAAGCTTTCTTTGAG) | |||
| Alignment with template seq. | 1st | é å解æãè¡ããªãã£ããµã³ãã«ã§ãã | 2nd | ã¢ããé ¸é å100%ä¸è´ |
| Alignment with TAIR7CDS | - | ã¢ããé ¸é å97.60%ä¸è´ | ||
| comment | 1st:(PCRã§ç®çãµã¤ãºã®å¢å¹ ã確èªã§ããããã³ããã¼ãå¾ãããªãã£ã)→2nd(09A03):Templateã®é åã¨ã¯ä¸è´ | |||
| sequence |
>IE_02F03_498bp ATGGCAGCGCGTGCCCATGATGTTGCGGCATTGAGTATCAAAGGAAGTTCCGCAATCCTTAACTTCCCTGAGCTCGCGGATTTTCTGCCAAGACCAGTCT CGCTCAGCCAACAGGATATCCAGGCCGCAGCCGCCGAAGCCGCTCTTATGGATTTCAAAACTGTACCATTCCATCTTCAGGATGACTCAACGCCGTTGCA AACTAGGTGTGATACTGAGAAGATCGAAAAGTGGTCATCCTCATCGTCCTCAGCCTCATCCTCATCCTCATCTTCGTCCTCGTCCTCATCATCTATGCTT TCGGGGGAGCTAGGAGATATTGTGGAGTTGCCGAGTCTTGAAAACAATGTAAAATACGATTGTGCGCTGTATGACTCGTTGGAGGGGCTGGTGTCGATGC CCCCATGGTTAGATGCTACCGAAAATGATTTTAGGTATGGAGATGATTCGGTACTGTTGGACCCATGTCTCAAAGAAAGCTTTTTGTGGAATTATGAG >IE_02F03_translated MAARAHDVAALSIKGSSAILNFPELADFLPRPVSLSQQDIQAAAAEAALMDFKTVPFHLQDDSTPLQTRCDTEKIEKWSSSSSSASSSSSSSSSSSSSML SGELGDIVELPSLENNVKYDCALYDSLEGLVSMPPWLDATENDFRYGDDSVLLDPCLKESFLWNYE |
|||
| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 498/675 (73.8%)（アミノ酸配列で166/225 (73.8%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT2G25 ATGGTGGATTCTCATGGCTCCGACACGGAATGTTCCTCCAAGAAGAAAAAGGAGAAAACGAAAGAAAAGGGGGTATATCGTGGGGCTCGCATGAGGAGCT
IE_02F ----------------------------------------------------------------------------------------------------
110 120 130 140 150 160 170 180 190 200
AT2G25 GGGGGAAATGGGTCTCGGAGATTCGGGAGCCCCGTAAGAAATCAAGAATCTGGCTCGGGACTTTCCCCACGGCGGAGATGGCAGCGCGTGCCCATGATGT
:::::::::::::::::::::::
IE_02F -----------------------------------------------------------------------------ATGGCAGCGCGTGCCCATGATGT
10 20
210 220 230 240 250 260 270 280 290 300
AT2G25 TGCGGCATTGAGTATCAAAGGAAGTTCCGCAATCCTTAACTTCCCTGAGCTCGCGGATTTTCTGCCAAGACCAGTCTCGCTCAGCCAACAGGATATCCAG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02F TGCGGCATTGAGTATCAAAGGAAGTTCCGCAATCCTTAACTTCCCTGAGCTCGCGGATTTTCTGCCAAGACCAGTCTCGCTCAGCCAACAGGATATCCAG
30 40 50 60 70 80 90 100 110 120
310 320 330 340 350 360 370 380 390 400
AT2G25 GCCGCAGCCGCCGAAGCCGCTCTTATGGATTTCAAAACTGTACCATTCCATCTTCAGGATGACTCAACGCCGTTGCAAACTAGGTGTGATACTGAGAAGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02F GCCGCAGCCGCCGAAGCCGCTCTTATGGATTTCAAAACTGTACCATTCCATCTTCAGGATGACTCAACGCCGTTGCAAACTAGGTGTGATACTGAGAAGA
130 140 150 160 170 180 190 200 210 220
410 420 430 440 450 460 470 480 490 500
AT2G25 TCGAAAAGTGGTCATCCTCATCGTCCTCAGCCTCATCCTCATCCTCATCTTCGTCCTCGTCCTCATCATCTATGCTTTCGGGGGAGCTAGGAGATATTGT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02F TCGAAAAGTGGTCATCCTCATCGTCCTCAGCCTCATCCTCATCCTCATCTTCGTCCTCGTCCTCATCATCTATGCTTTCGGGGGAGCTAGGAGATATTGT
230 240 250 260 270 280 290 300 310 320
510 520 530 540 550 560 570 580 590 600
AT2G25 GGAGTTGCCGAGTCTTGAAAACAATGTAAAATACGATTGTGCGCTGTATGACTCGTTGGAGGGGCTGGTGTCGATGCCCCCATGGTTAGATGCTACCGAA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02F GGAGTTGCCGAGTCTTGAAAACAATGTAAAATACGATTGTGCGCTGTATGACTCGTTGGAGGGGCTGGTGTCGATGCCCCCATGGTTAGATGCTACCGAA
330 340 350 360 370 380 390 400 410 420
610 620 630 640 650 660 670
AT2G25 AATGATTTTAGGTATGGAGATGATTCGGTACTGTTGGACCCATGTCTCAAAGAAAGCTTTTTGTGGAATTATGAG
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02F AATGATTTTAGGTATGGAGATGATTCGGTACTGTTGGACCCATGTCTCAAAGAAAGCTTTTTGTGGAATTATGAG
430 440 450 460 470 480 490
10 20 30 40 50 60 70 80 90 100
AT2G25 MVDSHGSDTECSSKKKKEKTKEKGVYRGARMRSWGKWVSEIREPRKKSRIWLGTFPTAEMAARAHDVAALSIKGSSAILNFPELADFLPRPVSLSQQDIQ
:::::::::::::::::::::::::::::::::::::::::
IE_02F -----------------------------------------------------------MAARAHDVAALSIKGSSAILNFPELADFLPRPVSLSQQDIQ
10 20 30 40
110 120 130 140 150 160 170 180 190 200
AT2G25 AAAAEAALMDFKTVPFHLQDDSTPLQTRCDTEKIEKWSSSSSSASSSSSSSSSSSSSMLSGELGDIVELPSLENNVKYDCALYDSLEGLVSMPPWLDATE
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_02F AAAAEAALMDFKTVPFHLQDDSTPLQTRCDTEKIEKWSSSSSSASSSSSSSSSSSSSMLSGELGDIVELPSLENNVKYDCALYDSLEGLVSMPPWLDATE
50 60 70 80 90 100 110 120 130 140
210 220
AT2G25 NDFRYGDDSVLLDPCLKESFLWNYE
:::::::::::::::::::::::::
IE_02F NDFRYGDDSVLLDPCLKESFLWNYE
150 160
|
|||