◆◆◆IE_03A05 detail◆◆◆

Code name	IE_03A05
Locus	AT4G36730
Template	IT_03A05 (HR0665)
Donor Vector	pDONR207
Forward primer	IPF_03A05 (ggggacaagtttgtacaaaaaagcaggcttcATGGGAACGAGCGAAGACAAGATGCCAT)
Reverse primer	IPR_03A05 (ggggaccactttgtacaagaaagctgggttATTTGTTCCTTCACCATCTTTCGACCCAGC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.40%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_03A05_939bp ATGGGAACGAGCGAAGACAAGATGCCATTTAAGACTACCAAACCAACATCTTCGGCTCAGGAAGTTCCTCCCACACCGTATCCAGATTGGCAAAATTCAA TGCAGGCTTATTATGGCGGAGGAGGTACTCCAAATCCTTTTTTCCCATCCCCAGTTGGATCTCCTAGTCCTCACCCCTATATGTGGGGTGCTCAACACCA TATGATGCCGCCTTATGGCACCCCAGTTCCGTACCCAGCAATGTATCCCCCGGGGGCAGTCTATGCTCATCCTAGCATGCCCATGCCTCCTAATTCTGGT CCTACCAACAAGGAGCCTGCGAAGGACCAAGCTTCTGGCAAGAAGTCAAAGGGGAACTCGAAAAAAAAGGCTGAAGGAGGTGATAAAGCGCTCTCTGGTT CAGGGAACGATGGTGCCTCTCATAGTGATGAAAGTGTCACAGCGGGTTCATCTGATGAAAATGATGAGAATGCCAATCAACAGGGTTCAATTCGAAAGCC AAGCTTTGGACAGATGCTTGCTGACGCAAGTTCTCAAAGTACGACTGGTGAAATCCAAGGTTCGGTGCCCATGAAGCCGGTAGCCCCGGGGACTAATCTG AATATCGGGATGGACTTATGGTCTTCCCAAGCTGGTGTACCAGTGAAGGATGAACGAGAGCTCAAGCGGCAGAAGAGGAAACAATCTAACCGTGAATCCG CTAGGCGGTCTAGATTGCGGAAGCAGGCCGAATGCGAACAACTTCAACAAAGAGTAGAGAGTTTGTCGAACGAGAATCAAAGCCTGAGAGATGAGCTACA GAGACTCTCAAGCGAATGTGATAAGCTCAAGTCTGAGAACAACTCAATCCAGGATGAGTTGCAGAGAGTACTTGGAGCAGAGGCTGTAGCTAATCTAGAA CAGAATGCTGCTGGGTCGAAAGATGGTGAAGGAACAAAT >IE_03A05_translated MGTSEDKMPFKTTKPTSSAQEVPPTPYPDWQNSMQAYYGGGGTPNPFFPSPVGSPSPHPYMWGAQHHMMPPYGTPVPYPAMYPPGAVYAHPSMPMPPNSG PTNKEPAKDQASGKKSKGNSKKKAEGGDKALSGSGNDGASHSDESVTAGSSDENDENANQQGSIRKPSFGQMLADASSQSTTGEIQGSVPMKPVAPGTNL NIGMDLWSSQAGVPVKDERELKRQKRKQSNRESARRSRLRKQAECEQLQQRVESLSNENQSLRDELQRLSSECDKLKSENNSIQDELQRVLGAEAVANLE QNAAGSKDGEGTN
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 939/945 (99.4%)（アミノ酸配列で313/315 (99.4%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G36 ATGGGAACGAGCGAAGACAAGATGCCATTTAAGACTACCAAACCAACATCTTCGGCTCAGGAAGTTCCTCCCACACCGTATCCAGATTGGCAAAATTCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A ATGGGAACGAGCGAAGACAAGATGCCATTTAAGACTACCAAACCAACATCTTCGGCTCAGGAAGTTCCTCCCACACCGTATCCAGATTGGCAAAATTCAA 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G36 TGCAGGCTTATTATGGCGGAGGAGGTACTCCAAATCCTTTTTTCCCATCCCCAGTTGGATCTCCTAGTCCTCACCCCTATATGTGGGGTGCTCAACACCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A TGCAGGCTTATTATGGCGGAGGAGGTACTCCAAATCCTTTTTTCCCATCCCCAGTTGGATCTCCTAGTCCTCACCCCTATATGTGGGGTGCTCAACACCA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G36 TATGATGCCGCCTTATGGCACCCCAGTTCCGTACCCAGCAATGTATCCCCCGGGGGCAGTCTATGCTCATCCTAGCATGCCCATGCCTCCTAATTCTGGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A TATGATGCCGCCTTATGGCACCCCAGTTCCGTACCCAGCAATGTATCCCCCGGGGGCAGTCTATGCTCATCCTAGCATGCCCATGCCTCCTAATTCTGGT 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT4G36 CCTACCAACAAGGAGCCTGCGAAGGACCAAGCTTCTGGCAAGAAGTCAAAGGGGAACTCGAAAAAAAAGGCTGAAGGAGGTGATAAAGCGCTCTCTGGTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A CCTACCAACAAGGAGCCTGCGAAGGACCAAGCTTCTGGCAAGAAGTCAAAGGGGAACTCGAAAAAAAAGGCTGAAGGAGGTGATAAAGCGCTCTCTGGTT 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT4G36 CAGGGAACGATGGTGCCTCTCATAGTGATGAAAGTGTCACAGCGGGTTCATCTGATGAAAATGATGAGAATGCCAATCAACAGGAACAGGGTTCAATTCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::: IE_03A CAGGGAACGATGGTGCCTCTCATAGTGATGAAAGTGTCACAGCGGGTTCATCTGATGAAAATGATGAGAATGCCAATC------AACAGGGTTCAATTCG 410 420 430 440 450 460 470 480 490 510 520 530 540 550 560 570 580 590 600 AT4G36 AAAGCCAAGCTTTGGACAGATGCTTGCTGACGCAAGTTCTCAAAGTACGACTGGTGAAATCCAAGGTTCGGTGCCCATGAAGCCGGTAGCCCCGGGGACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A AAAGCCAAGCTTTGGACAGATGCTTGCTGACGCAAGTTCTCAAAGTACGACTGGTGAAATCCAAGGTTCGGTGCCCATGAAGCCGGTAGCCCCGGGGACT 500 510 520 530 540 550 560 570 580 590 610 620 630 640 650 660 670 680 690 700 AT4G36 AATCTGAATATCGGGATGGACTTATGGTCTTCCCAAGCTGGTGTACCAGTGAAGGATGAACGAGAGCTCAAGCGGCAGAAGAGGAAACAATCTAACCGTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A AATCTGAATATCGGGATGGACTTATGGTCTTCCCAAGCTGGTGTACCAGTGAAGGATGAACGAGAGCTCAAGCGGCAGAAGAGGAAACAATCTAACCGTG 600 610 620 630 640 650 660 670 680 690 710 720 730 740 750 760 770 780 790 800 AT4G36 AATCCGCTAGGCGGTCTAGATTGCGGAAGCAGGCCGAATGCGAACAACTTCAACAAAGAGTAGAGAGTTTGTCGAACGAGAATCAAAGCCTGAGAGATGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A AATCCGCTAGGCGGTCTAGATTGCGGAAGCAGGCCGAATGCGAACAACTTCAACAAAGAGTAGAGAGTTTGTCGAACGAGAATCAAAGCCTGAGAGATGA 700 710 720 730 740 750 760 770 780 790 810 820 830 840 850 860 870 880 890 900 AT4G36 GCTACAGAGACTCTCAAGCGAATGTGATAAGCTCAAGTCTGAGAACAACTCAATCCAGGATGAGTTGCAGAGAGTACTTGGAGCAGAGGCTGTAGCTAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A GCTACAGAGACTCTCAAGCGAATGTGATAAGCTCAAGTCTGAGAACAACTCAATCCAGGATGAGTTGCAGAGAGTACTTGGAGCAGAGGCTGTAGCTAAT 800 810 820 830 840 850 860 870 880 890 910 920 930 940 AT4G36 CTAGAACAGAATGCTGCTGGGTCGAAAGATGGTGAAGGAACAAAT ::::::::::::::::::::::::::::::::::::::::::::: IE_03A CTAGAACAGAATGCTGCTGGGTCGAAAGATGGTGAAGGAACAAAT 900 910 920 930 10 20 30 40 50 60 70 80 90 100 AT4G36 MGTSEDKMPFKTTKPTSSAQEVPPTPYPDWQNSMQAYYGGGGTPNPFFPSPVGSPSPHPYMWGAQHHMMPPYGTPVPYPAMYPPGAVYAHPSMPMPPNSG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A MGTSEDKMPFKTTKPTSSAQEVPPTPYPDWQNSMQAYYGGGGTPNPFFPSPVGSPSPHPYMWGAQHHMMPPYGTPVPYPAMYPPGAVYAHPSMPMPPNSG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G36 PTNKEPAKDQASGKKSKGNSKKKAEGGDKALSGSGNDGASHSDESVTAGSSDENDENANQQEQGSIRKPSFGQMLADASSQSTTGEIQGSVPMKPVAPGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::: IE_03A PTNKEPAKDQASGKKSKGNSKKKAEGGDKALSGSGNDGASHSDESVTAGSSDENDENANQ--QGSIRKPSFGQMLADASSQSTTGEIQGSVPMKPVAPGT 110 120 130 140 150 160 170 180 190 210 220 230 240 250 260 270 280 290 300 AT4G36 NLNIGMDLWSSQAGVPVKDERELKRQKRKQSNRESARRSRLRKQAECEQLQQRVESLSNENQSLRDELQRLSSECDKLKSENNSIQDELQRVLGAEAVAN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A NLNIGMDLWSSQAGVPVKDERELKRQKRKQSNRESARRSRLRKQAECEQLQQRVESLSNENQSLRDELQRLSSECDKLKSENNSIQDELQRVLGAEAVAN 200 210 220 230 240 250 260 270 280 290 310 AT4G36 LEQNAAGSKDGEGTN ::::::::::::::: IE_03A LEQNAAGSKDGEGTN 300 310

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