◆◆◆IE_03A08 detail◆◆◆

Code name	IE_03A08
Locus	AT5G17810
Template	IT_03A08 (HR1076)
Donor Vector	pDONR207
Forward primer	IPF_03A08 (ggggacaagtttgtacaaaaaagcaggcttcATGAATCAAGAAGGTGCTTCACATAGCC)
Reverse primer	IPR_03A08 (ggggaccactttgtacaagaaagctgggttTGTCTGTCTCGGTACCAGGAAATAAGTTTG)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列100%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_03A08_804bp ATGAATCAAGAAGGTGCTTCACATAGCCCATCCTCCACTTCCACCGAACCAGTCCGGGCACGTTGGTCACCTAAACCGGAGCAAATCTTGATACTCGAAT CCATCTTCAACAGTGGTACTGTTAACCCACCAAAAGATGAAACGGTGAGGATAAGAAAGATGCTTGAGAAATTCGGTGCTGTGGGAGACGCAAACGTCTT CTACTGGTTTCAAAACCGACGGTCAAGATCTCGCCGGAGACACCGGCAGCTTTTAGCAGCCACCACCGCAGCCGCCACCTCCATAGGAGCTGAAGACCAC CAGCACATGACGGCCATGAGCATGCATCAATATCCTTGCAGCAACAACGAGATTGATTTGGGGTTTGGAAGTTGTAGCAACTTATCAGCTAATTACTTCC TTAATGGATCGTCGTCATCTCAAATCCCTTCCTTTTTCCTCGGCCTCTCTTCTTCAAGTGGTGGATGTGAGAACAACAATGGTATGGAGAATCTCTTCAA AATGTATGGCCATGAATCTGATCATAATCATCAGCAGCAGCATCATAGCTCAAATGCTGCATCAGTTTTAAACCCATCTGATCAAAACTCCAACTCCCAA TACGAACAAGAAGGGTTTATGACGGTGTTTATAAACGGAGTTCCTATGGAAGTAACAAAAGGAGCAATAGACATGAAAACAATGTTCGGTGATGATTCGG TGTTACTTCATTCCTCTGGTCTTCCTCTTCCCACTGATGAGTTTGGTTTCTTGATGCATTCTTTACAACATGGACAAACTTATTTCCTGGTACCGAGACA GACA >IE_03A08_translated MNQEGASHSPSSTSTEPVRARWSPKPEQILILESIFNSGTVNPPKDETVRIRKMLEKFGAVGDANVFYWFQNRRSRSRRRHRQLLAATTAAATSIGAEDH QHMTAMSMHQYPCSNNEIDLGFGSCSNLSANYFLNGSSSSQIPSFFLGLSSSSGGCENNNGMENLFKMYGHESDHNHQQQHHSSNAASVLNPSDQNSNSQ YEQEGFMTVFINGVPMEVTKGAIDMKTMFGDDSVLLHSSGLPLPTDEFGFLMHSLQHGQTYFLVPRQT
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1467/1467 (100.0%)（アミノ酸配列で100%）一致しました。 10 20 30 40 50 60 70 80 90 100 AT3G47 ATGCAAAAGCCAACATCAAGTATCTTAAATGTCATAATGGACGGTGGAGACAGCGTCGGAGGAGGAGGAGGAGATGATCACCACCGTCACCTCCACCATC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 ATGCAAAAGCCAACATCAAGTATCTTAAATGTCATAATGGACGGTGGAGACAGCGTCGGAGGAGGAGGAGGAGATGATCACCACCGTCACCTCCACCATC 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT3G47 ACCACCGCCCTACTTTCCCTTTTCAACTACTCGGAAAACACGACCCCGACGACAACCATCAACAACAACCTTCCCCTTCCTCCTCCTCCTCTCTATTTTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 ACCACCGCCCTACTTTCCCTTTTCAACTACTCGGAAAACACGACCCCGACGACAACCATCAACAACAACCTTCCCCTTCCTCCTCCTCCTCTCTATTTTC 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT3G47 TCTCCATCAACACCAACAATTATCTCAATCGCAACCTCAATCGCAATCGCAAAAGTCACAACCGCAGACAACGCAAAAAGAGTTATTACAAACGCAAGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 TCTCCATCAACACCAACAATTATCTCAATCGCAACCTCAATCGCAATCGCAAAAGTCACAACCGCAGACAACGCAAAAAGAGTTATTACAAACGCAAGAG 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT3G47 GAATCTGCGGTGGTGGCAGCTAAAAAGCCACCGTTGAAACGAGCGTCGACGAAAGACCGACACACGAAAGTAGACGGAAGAGGGAGGAGAATAAGGATGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 GAATCTGCGGTGGTGGCAGCTAAAAAGCCACCGTTGAAACGAGCGTCGACGAAAGACCGACACACGAAAGTAGACGGAAGAGGGAGGAGAATAAGGATGC 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT3G47 CGGCGTTATGTGCAGCTAGGGTTTTTCAGCTAACGCGAGAGCTAGGTCATAAATCCGACGGTGAGACAATAGAGTGGCTTCTTCAACAAGCTGAACCATC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 CGGCGTTATGTGCAGCTAGGGTTTTTCAGCTAACGCGAGAGCTAGGTCATAAATCCGACGGTGAGACAATAGAGTGGCTTCTTCAACAAGCTGAACCATC 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT3G47 TGTAATCGCCGCCACCGGAACCGGAACAATCCCGGCGAATTTCACTTCTTTAAACATCTCTCTCCGTTCTTCAGGCTCTTCCATGTCTCTTCCTTCTCAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 TGTAATCGCCGCCACCGGAACCGGAACAATCCCGGCGAATTTCACTTCTTTAAACATCTCTCTCCGTTCTTCAGGCTCTTCCATGTCTCTTCCTTCTCAT 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT3G47 TTCCGCTCCGCCGCTTCCACTTTTAGCCCTAATAACATATTTTCTCCGGCGATGCTTCAACAACAACAACAACAACAACGTGGTGGTGGTGTTGGGTTTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 TTCCGCTCCGCCGCTTCCACTTTTAGCCCTAATAACATATTTTCTCCGGCGATGCTTCAACAACAACAACAACAACAACGTGGTGGTGGTGTTGGGTTTC 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT3G47 ATCATCCCCATCTACAGGGACGTGCACCTACGTCGTCTTTGTTTCCTGGTATTGATAACTTCACACCAACGACGTCGTTTTTGAACTTTCATAATCCAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 ATCATCCCCATCTACAGGGACGTGCACCTACGTCGTCTTTGTTTCCTGGTATTGATAACTTCACACCAACGACGTCGTTTTTGAACTTTCATAATCCAAC 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT3G47 AAAGCAAGAAGGAGATCAAGATTCTGAAGAGTTAAACTCGGAGAAGAAAAGAAGAATCCAAACGACGTCGGATTTGCATCAACAACAACAACAACACCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 AAAGCAAGAAGGAGATCAAGATTCTGAAGAGTTAAACTCGGAGAAGAAAAGAAGAATCCAAACGACGTCGGATTTGCATCAACAACAACAACAACACCAA 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT3G47 CATGATCAAATCGGAGGATATACACTTCAATCTAGCAACAGTGGATCTACAGCCACCGCAGCCGCCGCGCAACAAATACCGGGAAATTTCTGGATGGTTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 CATGATCAAATCGGAGGATATACACTTCAATCTAGCAACAGTGGATCTACAGCCACCGCAGCCGCCGCGCAACAAATACCGGGAAATTTCTGGATGGTTG 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT3G47 CGGCGGCTGCGGCTGCAGGTGGTGGTGGTGGTAATAACAACCAAACAGGTGGTCTTATGACAGCTTCTATTGGTACTGGTGGCGGCGGTGGAGAGCCTGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 CGGCGGCTGCGGCTGCAGGTGGTGGTGGTGGTAATAACAACCAAACAGGTGGTCTTATGACAGCTTCTATTGGTACTGGTGGCGGCGGTGGAGAGCCTGT 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT3G47 TTGGACGTTTCCTTCCATTAACACGGCAGCGGCAGCGTTATATAGAAGTGGCGTTTCGGGCGTTCCAAGCGGCGCGGTTTCTAGCGGTTTACATTTTATG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 TTGGACGTTTCCTTCCATTAACACGGCAGCGGCAGCGTTATATAGAAGTGGCGTTTCGGGCGTTCCAAGCGGCGCGGTTTCTAGCGGTTTACATTTTATG 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT3G47 AATTTCGCAGCGCCAATGGCATTTCTTACTGGACAACAACAGCTAGCAACAACTAGTAATCATGAGATTAATGAAGATAGTAATAATAATGAAGGAGGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 AATTTCGCAGCGCCAATGGCATTTCTTACTGGACAACAACAGCTAGCAACAACTAGTAATCATGAGATTAATGAAGATAGTAATAATAATGAAGGAGGAA 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT3G47 GAAGTGACGGTGGTGGTGATCATCATAATACACAGAGACATCATCATCATCAACAACAACATCATCATAATATTCTCTCCGGCTTGAACCAGTACGGACG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 GAAGTGACGGTGGTGGTGATCATCATAATACACAGAGACATCATCATCATCAACAACAACATCATCATAATATTCTCTCCGGCTTGAACCAGTACGGACG 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 AT3G47 GCAAGTTTCCGGCGACTCTCAAGCTAGTGGATCACTTGGAGGTGGTGATGAGGAGGATCAGCAAGAT ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 GCAAGTTTCCGGCGACTCTCAAGCTAGTGGATCACTTGGAGGTGGTGATGAGGAGGATCAGCAAGAT 1410 1420 1430 1440 1450 1460 10 20 30 40 50 60 70 80 90 100 AT3G47 MQKPTSSILNVIMDGGDSVGGGGGDDHHRHLHHHHRPTFPFQLLGKHDPDDNHQQQPSPSSSSSLFSLHQHQQLSQSQPQSQSQKSQPQTTQKELLQTQE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 MQKPTSSILNVIMDGGDSVGGGGGDDHHRHLHHHHRPTFPFQLLGKHDPDDNHQQQPSPSSSSSLFSLHQHQQLSQSQPQSQSQKSQPQTTQKELLQTQE 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT3G47 ESAVVAAKKPPLKRASTKDRHTKVDGRGRRIRMPALCAARVFQLTRELGHKSDGETIEWLLQQAEPSVIAATGTGTIPANFTSLNISLRSSGSSMSLPSH :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 ESAVVAAKKPPLKRASTKDRHTKVDGRGRRIRMPALCAARVFQLTRELGHKSDGETIEWLLQQAEPSVIAATGTGTIPANFTSLNISLRSSGSSMSLPSH 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT3G47 FRSAASTFSPNNIFSPAMLQQQQQQQRGGGVGFHHPHLQGRAPTSSLFPGIDNFTPTTSFLNFHNPTKQEGDQDSEELNSEKKRRIQTTSDLHQQQQQHQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 FRSAASTFSPNNIFSPAMLQQQQQQQRGGGVGFHHPHLQGRAPTSSLFPGIDNFTPTTSFLNFHNPTKQEGDQDSEELNSEKKRRIQTTSDLHQQQQQHQ 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT3G47 HDQIGGYTLQSSNSGSTATAAAAQQIPGNFWMVAAAAAAGGGGGNNNQTGGLMTASIGTGGGGGEPVWTFPSINTAAAALYRSGVSGVPSGAVSSGLHFM :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 HDQIGGYTLQSSNSGSTATAAAAQQIPGNFWMVAAAAAAGGGGGNNNQTGGLMTASIGTGGGGGEPVWTFPSINTAAAALYRSGVSGVPSGAVSSGLHFM 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 AT3G47 NFAAPMAFLTGQQQLATTSNHEINEDSNNNEGGRSDGGGDHHNTQRHHHHQQQHHHNILSGLNQYGRQVSGDSQASGSLGGGDEEDQQD ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: RE_100 NFAAPMAFLTGQQQLATTSNHEINEDSNNNEGGRSDGGGDHHNTQRHHHHQQQHHHNILSGLNQYGRQVSGDSQASGSLGGGDEEDQQD 410 420 430 440 450 460 470 480

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