◆◆◆IE_03A08 detail◆◆◆

Code name	IE_03A08
Locus	AT5G17810
Template	IT_03A08 (HR1076)
Donor Vector	pDONR207
Forward primer	IPF_03A08 (ggggacaagtttgtacaaaaaagcaggcttcATGAATCAAGAAGGTGCTTCACATAGCC)
Reverse primer	IPR_03A08 (ggggaccactttgtacaagaaagctgggttTGTCTGTCTCGGTACCAGGAAATAAGTTTG)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列100%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_03A08_804bp ATGAATCAAGAAGGTGCTTCACATAGCCCATCCTCCACTTCCACCGAACCAGTCCGGGCACGTTGGTCACCTAAACCGGAGCAAATCTTGATACTCGAAT CCATCTTCAACAGTGGTACTGTTAACCCACCAAAAGATGAAACGGTGAGGATAAGAAAGATGCTTGAGAAATTCGGTGCTGTGGGAGACGCAAACGTCTT CTACTGGTTTCAAAACCGACGGTCAAGATCTCGCCGGAGACACCGGCAGCTTTTAGCAGCCACCACCGCAGCCGCCACCTCCATAGGAGCTGAAGACCAC CAGCACATGACGGCCATGAGCATGCATCAATATCCTTGCAGCAACAACGAGATTGATTTGGGGTTTGGAAGTTGTAGCAACTTATCAGCTAATTACTTCC TTAATGGATCGTCGTCATCTCAAATCCCTTCCTTTTTCCTCGGCCTCTCTTCTTCAAGTGGTGGATGTGAGAACAACAATGGTATGGAGAATCTCTTCAA AATGTATGGCCATGAATCTGATCATAATCATCAGCAGCAGCATCATAGCTCAAATGCTGCATCAGTTTTAAACCCATCTGATCAAAACTCCAACTCCCAA TACGAACAAGAAGGGTTTATGACGGTGTTTATAAACGGAGTTCCTATGGAAGTAACAAAAGGAGCAATAGACATGAAAACAATGTTCGGTGATGATTCGG TGTTACTTCATTCCTCTGGTCTTCCTCTTCCCACTGATGAGTTTGGTTTCTTGATGCATTCTTTACAACATGGACAAACTTATTTCCTGGTACCGAGACA GACA >IE_03A08_translated MNQEGASHSPSSTSTEPVRARWSPKPEQILILESIFNSGTVNPPKDETVRIRKMLEKFGAVGDANVFYWFQNRRSRSRRRHRQLLAATTAAATSIGAEDH QHMTAMSMHQYPCSNNEIDLGFGSCSNLSANYFLNGSSSSQIPSFFLGLSSSSGGCENNNGMENLFKMYGHESDHNHQQQHHSSNAASVLNPSDQNSNSQ YEQEGFMTVFINGVPMEVTKGAIDMKTMFGDDSVLLHSSGLPLPTDEFGFLMHSLQHGQTYFLVPRQT
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 803/804 (99.9%)（アミノ酸配列で100%）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G17 ATGAATCAAGAAGGTGCTTCACATAGCCCATCCTCCACTTCCACCGAACCAGTCCGGGCACGTTGGTCACCTAAACCGGAGCAAATCTTGATACTCGAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A ATGAATCAAGAAGGTGCTTCACATAGCCCATCCTCCACTTCCACCGAACCAGTCCGGGCACGTTGGTCACCTAAACCGGAGCAAATCTTGATACTCGAAT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G17 CCATCTTCAACAGTGGTACTGTTAACCCACCAAAAGATGAAACGGTGAGGATAAGAAAGATGCTTGAGAAATTCGGTGCTGTGGGAGACGCAAACGTCTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A CCATCTTCAACAGTGGTACTGTTAACCCACCAAAAGATGAAACGGTGAGGATAAGAAAGATGCTTGAGAAATTCGGTGCTGTGGGAGACGCAAACGTCTT 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G17 CTACTGGTTTCAAAACCGACGGTCAAGATCTCGCCGGAGACACCGGCAGCTTTTAGCAGCCACCACCGCAGCCGCCACCTCCATAGGAGCTGAAGACCAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A CTACTGGTTTCAAAACCGACGGTCAAGATCTCGCCGGAGACACCGGCAGCTTTTAGCAGCCACCACCGCAGCCGCCACCTCCATAGGAGCTGAAGACCAC 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G17 CAGCACATGACGGCCATGAGCATGCATCAATATCCTTGCAGCAACAACGAGATTGATTTGGGGTTTGGAAGTTGTAGCAACTTATCAGCTAATTACTTCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A CAGCACATGACGGCCATGAGCATGCATCAATATCCTTGCAGCAACAACGAGATTGATTTGGGGTTTGGAAGTTGTAGCAACTTATCAGCTAATTACTTCC 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G17 TTAATGGATCGTCGTCATCTCAAATCCCTTCCTTTTTCCTCGGCCTCTCTTCTTCAAGTGGTGGGTGTGAGAACAACAATGGTATGGAGAATCTCTTCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::: IE_03A TTAATGGATCGTCGTCATCTCAAATCCCTTCCTTTTTCCTCGGCCTCTCTTCTTCAAGTGGTGGATGTGAGAACAACAATGGTATGGAGAATCTCTTCAA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G17 AATGTATGGCCATGAATCTGATCATAATCATCAGCAGCAGCATCATAGCTCAAATGCTGCATCAGTTTTAAACCCATCTGATCAAAACTCCAACTCCCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A AATGTATGGCCATGAATCTGATCATAATCATCAGCAGCAGCATCATAGCTCAAATGCTGCATCAGTTTTAAACCCATCTGATCAAAACTCCAACTCCCAA 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G17 TACGAACAAGAAGGGTTTATGACGGTGTTTATAAACGGAGTTCCTATGGAAGTAACAAAAGGAGCAATAGACATGAAAACAATGTTCGGTGATGATTCGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A TACGAACAAGAAGGGTTTATGACGGTGTTTATAAACGGAGTTCCTATGGAAGTAACAAAAGGAGCAATAGACATGAAAACAATGTTCGGTGATGATTCGG 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT5G17 TGTTACTTCATTCCTCTGGTCTTCCTCTTCCCACTGATGAGTTTGGTTTCTTGATGCATTCTTTACAACATGGACAAACTTATTTCCTGGTACCGAGACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A TGTTACTTCATTCCTCTGGTCTTCCTCTTCCCACTGATGAGTTTGGTTTCTTGATGCATTCTTTACAACATGGACAAACTTATTTCCTGGTACCGAGACA 710 720 730 740 750 760 770 780 790 800 AT5G17 GACA :::: IE_03A GACA 10 20 30 40 50 60 70 80 90 100 AT5G17 MNQEGASHSPSSTSTEPVRARWSPKPEQILILESIFNSGTVNPPKDETVRIRKMLEKFGAVGDANVFYWFQNRRSRSRRRHRQLLAATTAAATSIGAEDH :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A MNQEGASHSPSSTSTEPVRARWSPKPEQILILESIFNSGTVNPPKDETVRIRKMLEKFGAVGDANVFYWFQNRRSRSRRRHRQLLAATTAAATSIGAEDH 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G17 QHMTAMSMHQYPCSNNEIDLGFGSCSNLSANYFLNGSSSSQIPSFFLGLSSSSGGCENNNGMENLFKMYGHESDHNHQQQHHSSNAASVLNPSDQNSNSQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A QHMTAMSMHQYPCSNNEIDLGFGSCSNLSANYFLNGSSSSQIPSFFLGLSSSSGGCENNNGMENLFKMYGHESDHNHQQQHHSSNAASVLNPSDQNSNSQ 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 AT5G17 YEQEGFMTVFINGVPMEVTKGAIDMKTMFGDDSVLLHSSGLPLPTDEFGFLMHSLQHGQTYFLVPRQT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03A YEQEGFMTVFINGVPMEVTKGAIDMKTMFGDDSVLLHSSGLPLPTDEFGFLMHSLQHGQTYFLVPRQT 210 220 230 240 250 260

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