◆◆◆IE_03B09 detail◆◆◆

Code name	IE_03B09
Locus	AT3G28470
Template	IT_03B09 (HR1796)
Donor Vector	pDONR207
Forward primer	IPF_03B09 (ggggacaagtttgtacaaaaaagcaggcttcATGGGAAGACCTCCTTGTTGTGACAAGT)
Reverse primer	IPR_03B09 (ggggaccactttgtacaagaaagctgggttATAATCGAAATCATTCAAGAGTTGAGGAAA)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.70%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_03B09_951bp ATGGGAAGACCTCCTTGTTGTGACAAGTCCAATGTCAAGAAAGGTCTCTGGACCGAGGAAGAAGACGCTAAGATCCTTGCTTATGTTGCTATCCATGGTG TAGGAAACTGGAGCTTGATCCCCAAAAAAGCAGGTCTGAATCGATGTGGAAAGAGCTGTAGACTAAGATGGACTAATTACTTAAGACCTGACCTTAAACA TGACAGCTTCTCTACCCAAGAAGAAGAGCTTATCATTGAGTGTCATAGAGCCATTGGCAGCAGGTGGTCTTCCATTGCACGAAAGCTTCCAGGAAGAGCG GATAATGATGTGAAGAATCACTGGAACACAAAGCTGAAGAAGAAGCTGATGAAAATGGGGATAGACCCGGTGACTCATAAACCGGTTTCTCAACTCCTTG CAGAATTCAGAAACATTAGCGGCCATGGAAATGCATCCTTCAAAACAGAACCATCTAACAACTCTATACTCACACAATCCAACTCAGCTTGGGAAATGAT GAGAAACACAACAACAAACCATGAGAGTTATTACACCAACTCTCCAATGATGTTTACAAATTCCTCTGAGTACCAAACTACTCCATTTCATTTCTATAGC CATCCAAATCATCTGCTCAATGGAACCACATCTTCATGCTCTTCCTCATCATCTTCTACTAGTATCACTCAGCCAAACCAAGTACCTCAAACACCGGTTA CTAACTTCTACTGGAGCGATTTCCTTCTCTCGGACCCGGTTCCTCAAGTAGTGGGATCCTCAGCTACTAGCGACCTCACTTTTACGCAGAACGAACATCA TTTCAACATCGAAGCCGAATACATCTCTCAAAACATCGATTCAAAGGCCTCGGGAACATGTCATTCCGCGAGTTCCTTCGTTGACGAAATACTAGATAAA GACCAAGAGATGTTGTCACAGTTTCCTCAACTCTTGAATGATTTCGATTAT >IE_03B09_translated MGRPPCCDKSNVKKGLWTEEEDAKILAYVAIHGVGNWSLIPKKAGLNRCGKSCRLRWTNYLRPDLKHDSFSTQEEELIIECHRAIGSRWSSIARKLPGRA DNDVKNHWNTKLKKKLMKMGIDPVTHKPVSQLLAEFRNISGHGNASFKTEPSNNSILTQSNSAWEMMRNTTTNHESYYTNSPMMFTNSSEYQTTPFHFYS HPNHLLNGTTSSCSSSSSSTSITQPNQVPQTPVTNFYWSDFLLSDPVPQVVGSSATSDLTFTQNEHHFNIEAEYISQNIDSKASGTCHSASSFVDEILDK DQEMLSQFPQLLNDFDY
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 950/951 (99.9%)（アミノ酸配列で316/317 (99.7%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT3G28 ATGGGAAGACCTCCTTGTTGTGACAAGTCCAATGTCAAGAAAGGTCTCTGGACCGAGGAAGAAGACGCTAAGATCCTTGCTTATGTTGCTATCCATGGTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B ATGGGAAGACCTCCTTGTTGTGACAAGTCCAATGTCAAGAAAGGTCTCTGGACCGAGGAAGAAGACGCTAAGATCCTTGCTTATGTTGCTATCCATGGTG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT3G28 TAGGAAACTGGAGCTTGATCCCCAAAAAAGCAGGTCTGAATCGATGTGGAAAGAGCTGTAGACTAAGATGGACTAATTACTTAAGACCTGACCTTAAACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B TAGGAAACTGGAGCTTGATCCCCAAAAAAGCAGGTCTGAATCGATGTGGAAAGAGCTGTAGACTAAGATGGACTAATTACTTAAGACCTGACCTTAAACA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT3G28 TGACAGCTTCTCTACCCAAGAAGAAGAGCTTATCATTGAGTGTCATAGAGCCATTGGCAGCAGGTGGTCTTCCATTGCACGAAAGCTTCCAGGAAGAACG ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :: IE_03B TGACAGCTTCTCTACCCAAGAAGAAGAGCTTATCATTGAGTGTCATAGAGCCATTGGCAGCAGGTGGTCTTCCATTGCACGAAAGCTTCCAGGAAGAGCG 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT3G28 GATAATGATGTGAAGAATCACTGGAACACAAAGCTGAAGAAGAAGCTGATGAAAATGGGGATAGACCCGGTGACTCATAAACCGGTTTCTCAACTCCTTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B GATAATGATGTGAAGAATCACTGGAACACAAAGCTGAAGAAGAAGCTGATGAAAATGGGGATAGACCCGGTGACTCATAAACCGGTTTCTCAACTCCTTG 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT3G28 CAGAATTCAGAAACATTAGCGGCCATGGAAATGCATCCTTCAAAACAGAACCATCTAACAACTCTATACTCACACAATCCAACTCAGCTTGGGAAATGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B CAGAATTCAGAAACATTAGCGGCCATGGAAATGCATCCTTCAAAACAGAACCATCTAACAACTCTATACTCACACAATCCAACTCAGCTTGGGAAATGAT 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT3G28 GAGAAACACAACAACAAACCATGAGAGTTATTACACCAACTCTCCAATGATGTTTACAAATTCCTCTGAGTACCAAACTACTCCATTTCATTTCTATAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B GAGAAACACAACAACAAACCATGAGAGTTATTACACCAACTCTCCAATGATGTTTACAAATTCCTCTGAGTACCAAACTACTCCATTTCATTTCTATAGC 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT3G28 CATCCAAATCATCTGCTCAATGGAACCACATCTTCATGCTCTTCCTCATCATCTTCTACTAGTATCACTCAGCCAAACCAAGTACCTCAAACACCGGTTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B CATCCAAATCATCTGCTCAATGGAACCACATCTTCATGCTCTTCCTCATCATCTTCTACTAGTATCACTCAGCCAAACCAAGTACCTCAAACACCGGTTA 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT3G28 CTAACTTCTACTGGAGCGATTTCCTTCTCTCGGACCCGGTTCCTCAAGTAGTGGGATCCTCAGCTACTAGCGACCTCACTTTTACGCAGAACGAACATCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B CTAACTTCTACTGGAGCGATTTCCTTCTCTCGGACCCGGTTCCTCAAGTAGTGGGATCCTCAGCTACTAGCGACCTCACTTTTACGCAGAACGAACATCA 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT3G28 TTTCAACATCGAAGCCGAATACATCTCTCAAAACATCGATTCAAAGGCCTCGGGAACATGTCATTCCGCGAGTTCCTTCGTTGACGAAATACTAGATAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B TTTCAACATCGAAGCCGAATACATCTCTCAAAACATCGATTCAAAGGCCTCGGGAACATGTCATTCCGCGAGTTCCTTCGTTGACGAAATACTAGATAAA 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 AT3G28 GACCAAGAGATGTTGTCACAGTTTCCTCAACTCTTGAATGATTTCGATTAT ::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B GACCAAGAGATGTTGTCACAGTTTCCTCAACTCTTGAATGATTTCGATTAT 910 920 930 940 950 10 20 30 40 50 60 70 80 90 100 AT3G28 MGRPPCCDKSNVKKGLWTEEEDAKILAYVAIHGVGNWSLIPKKAGLNRCGKSCRLRWTNYLRPDLKHDSFSTQEEELIIECHRAIGSRWSSIARKLPGRT ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B MGRPPCCDKSNVKKGLWTEEEDAKILAYVAIHGVGNWSLIPKKAGLNRCGKSCRLRWTNYLRPDLKHDSFSTQEEELIIECHRAIGSRWSSIARKLPGRA 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT3G28 DNDVKNHWNTKLKKKLMKMGIDPVTHKPVSQLLAEFRNISGHGNASFKTEPSNNSILTQSNSAWEMMRNTTTNHESYYTNSPMMFTNSSEYQTTPFHFYS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B DNDVKNHWNTKLKKKLMKMGIDPVTHKPVSQLLAEFRNISGHGNASFKTEPSNNSILTQSNSAWEMMRNTTTNHESYYTNSPMMFTNSSEYQTTPFHFYS 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT3G28 HPNHLLNGTTSSCSSSSSSTSITQPNQVPQTPVTNFYWSDFLLSDPVPQVVGSSATSDLTFTQNEHHFNIEAEYISQNIDSKASGTCHSASSFVDEILDK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B HPNHLLNGTTSSCSSSSSSTSITQPNQVPQTPVTNFYWSDFLLSDPVPQVVGSSATSDLTFTQNEHHFNIEAEYISQNIDSKASGTCHSASSFVDEILDK 210 220 230 240 250 260 270 280 290 300 310 AT3G28 DQEMLSQFPQLLNDFDY ::::::::::::::::: IE_03B DQEMLSQFPQLLNDFDY 310

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