◆◆◆IE_03B11 detail◆◆◆

Code name	IE_03B11
Locus	AT1G25340
Template	IT_03B11 (HR1990)
Donor Vector	pDONR207
Forward primer	IPF_03B11 (ggggacaagtttgtacaaaaaagcaggcttcATGTCAAATATAACAAAGAAGAAGTGTA)
Reverse primer	IPR_03B11 (ggggaccactttgtacaagaaagctgggttATTAAAGTGTGCATACTCGTTAAACTGCCA)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.60%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_03B11_849bp ATGTCAAATATAACAAAGAAGAAGTGTAATGGAAATGAAGAGGGTGCAGAGCAGAGGAAAGGGCCTTGGACACTCGAGGAAGACACTCTTCTCACCAATT ACATTTCCCATAACGGTGAGGGCCGATGGAATCTGCTCGCTAAATCTTCTGGGCTAAAGAGAGCAGGAAAAAGTTGTAGATTGAGATGGTTGAATTACCT TAAACCCGACATAAAGCGTGGGAATCTCACTCCTCGAGAACAACTTTTAATCCTTGAGCTCCATTCTAAATGGGGTAATAGGTGGTCAAAAATTTCGAAG TATTTACCAGGAAGAACAGACAACGATATCAAAAACTACTGGAGAACTAGAGTCCAGAAACAAGCACGCCAGCTCAACATAGATTCCAATAGCCACAAGT TCATAGAAGTTGTTCGTAGCTTTTGGTTTCCAAGACTGATCAACGAGATTAAAGACAACTCATACACCAACAATATTAAAGCTAATGCTCCTGATTTACT TGGACCAATTTTACGAGACAGCAAAGATTTGGGTTTCAACAACATGGATTGTTCCACTTCCATGTCAGAAGATCTCAAGAAAACTTCACAATTCATGGAT TTTTCTGATCTTGAAACCACAATGTCCTTGGAAGGATCACGAGGGGGTAGTAGTCAATGTGTGAGTGAGGTTTATAGCTCCTTCCCTTGCCTAGAGGAGG AGTACATGGTGGCCGTTATGGGCAGTTCAGACATTTCAGCATTGCATGATTGTCACGTGGCTGATTCCAAGTACGAGGATGATGTGACACAAGATCTAAT GTGGAACATGGATGACATTTGGCAGTTTAACGAGTATGCACACTTTAAT >IE_03B11_translated MSNITKKKCNGNEEGAEQRKGPWTLEEDTLLTNYISHNGEGRWNLLAKSSGLKRAGKSCRLRWLNYLKPDIKRGNLTPREQLLILELHSKWGNRWSKISK YLPGRTDNDIKNYWRTRVQKQARQLNIDSNSHKFIEVVRSFWFPRLINEIKDNSYTNNIKANAPDLLGPILRDSKDLGFNNMDCSTSMSEDLKKTSQFMD FSDLETTMSLEGSRGGSSQCVSEVYSSFPCLEEEYMVAVMGSSDISALHDCHVADSKYEDDVTQDLMWNMDDIWQFNEYAHFN
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 847/849 (99.8%)（アミノ酸配列で282/283 (99.6%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT1G25 ATGTCAAATATAACAAAGAAGAAGTGTAATGGAAATGAAGAGGGTGCAGAGCAGAGGAAAGGGCCTTGGACACTCGAGGAAGACACTCTTCTCACCAATT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B ATGTCAAATATAACAAAGAAGAAGTGTAATGGAAATGAAGAGGGTGCAGAGCAGAGGAAAGGGCCTTGGACACTCGAGGAAGACACTCTTCTCACCAATT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G25 ACATTTCCCATAACGGTGAAGGCCGATGGAATCTGCTCGCTAAATCTTCTGGGCTAAAGAGAGCAGGAAAAAGTTGTAGATTGAGATGGTTGAATTACCT ::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B ACATTTCCCATAACGGTGAGGGCCGATGGAATCTGCTCGCTAAATCTTCTGGGCTAAAGAGAGCAGGAAAAAGTTGTAGATTGAGATGGTTGAATTACCT 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT1G25 TAAACCCGACATAAAGCGTGGGAATCTCACTCCTCAAGAACAACTTTTAATCCTTGAGCTCCATTCTAAATGGGGTAATAGGTGGTCAAAAATTTCGAAG ::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B TAAACCCGACATAAAGCGTGGGAATCTCACTCCTCGAGAACAACTTTTAATCCTTGAGCTCCATTCTAAATGGGGTAATAGGTGGTCAAAAATTTCGAAG 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT1G25 TATTTACCAGGAAGAACAGACAACGATATCAAAAACTACTGGAGAACTAGAGTCCAGAAACAAGCACGCCAGCTCAACATAGATTCCAATAGCCACAAGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B TATTTACCAGGAAGAACAGACAACGATATCAAAAACTACTGGAGAACTAGAGTCCAGAAACAAGCACGCCAGCTCAACATAGATTCCAATAGCCACAAGT 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT1G25 TCATAGAAGTTGTTCGTAGCTTTTGGTTTCCAAGACTGATCAACGAGATTAAAGACAACTCATACACCAACAATATTAAAGCTAATGCTCCTGATTTACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B TCATAGAAGTTGTTCGTAGCTTTTGGTTTCCAAGACTGATCAACGAGATTAAAGACAACTCATACACCAACAATATTAAAGCTAATGCTCCTGATTTACT 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT1G25 TGGACCAATTTTACGAGACAGCAAAGATTTGGGTTTCAACAACATGGATTGTTCCACTTCCATGTCAGAAGATCTCAAGAAAACTTCACAATTCATGGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B TGGACCAATTTTACGAGACAGCAAAGATTTGGGTTTCAACAACATGGATTGTTCCACTTCCATGTCAGAAGATCTCAAGAAAACTTCACAATTCATGGAT 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT1G25 TTTTCTGATCTTGAAACCACAATGTCCTTGGAAGGATCACGAGGGGGTAGTAGTCAATGTGTGAGTGAGGTTTATAGCTCCTTCCCTTGCCTAGAGGAGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B TTTTCTGATCTTGAAACCACAATGTCCTTGGAAGGATCACGAGGGGGTAGTAGTCAATGTGTGAGTGAGGTTTATAGCTCCTTCCCTTGCCTAGAGGAGG 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT1G25 AGTACATGGTGGCCGTTATGGGCAGTTCAGACATTTCAGCATTGCATGATTGTCACGTGGCTGATTCCAAGTACGAGGATGATGTGACACAAGATCTAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B AGTACATGGTGGCCGTTATGGGCAGTTCAGACATTTCAGCATTGCATGATTGTCACGTGGCTGATTCCAAGTACGAGGATGATGTGACACAAGATCTAAT 710 720 730 740 750 760 770 780 790 800 810 820 830 840 AT1G25 GTGGAACATGGATGACATTTGGCAGTTTAACGAGTATGCACACTTTAAT ::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B GTGGAACATGGATGACATTTGGCAGTTTAACGAGTATGCACACTTTAAT 810 820 830 840 10 20 30 40 50 60 70 80 90 100 AT1G25 MSNITKKKCNGNEEGAEQRKGPWTLEEDTLLTNYISHNGEGRWNLLAKSSGLKRAGKSCRLRWLNYLKPDIKRGNLTPQEQLLILELHSKWGNRWSKISK ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::.::::::::::::::::::::: IE_03B MSNITKKKCNGNEEGAEQRKGPWTLEEDTLLTNYISHNGEGRWNLLAKSSGLKRAGKSCRLRWLNYLKPDIKRGNLTPREQLLILELHSKWGNRWSKISK 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G25 YLPGRTDNDIKNYWRTRVQKQARQLNIDSNSHKFIEVVRSFWFPRLINEIKDNSYTNNIKANAPDLLGPILRDSKDLGFNNMDCSTSMSEDLKKTSQFMD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B YLPGRTDNDIKNYWRTRVQKQARQLNIDSNSHKFIEVVRSFWFPRLINEIKDNSYTNNIKANAPDLLGPILRDSKDLGFNNMDCSTSMSEDLKKTSQFMD 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 AT1G25 FSDLETTMSLEGSRGGSSQCVSEVYSSFPCLEEEYMVAVMGSSDISALHDCHVADSKYEDDVTQDLMWNMDDIWQFNEYAHFN ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03B FSDLETTMSLEGSRGGSSQCVSEVYSSFPCLEEEYMVAVMGSSDISALHDCHVADSKYEDDVTQDLMWNMDDIWQFNEYAHFN 210 220 230 240 250 260 270 280

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