◆◆◆IE_03E07 detail◆◆◆

Code name	IE_03E07
Locus	AT1G54330
Template	IT_03E07 (HR3061)
Donor Vector	pDONR207
Forward primer	IPF_03E07 (ggggacaagtttgtacaaaaaagcaggcttcATGAGTTTGCCTCCAGGATTCAGGTTTC)
Reverse primer	IPR_03E07 (ggggaccactttgtacaagaaagctgggttAAGGGAAGCGGGGAAAGTGGCGGAAGAAGA)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.00%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_03E07_894bp ATGAGTTTGCCTCCAGGATTCAGGTTTCATCCCACTGATGAAGAACTGGTGGCTTACTATCTTGATAGGAAGGTCAACGGCCAAGCCATTGAGCTCGAGA TCATCCCAGAAGTTGATCTTTATAAATGCGAGCCATGGGACTTGCCTGAAAAGTCATTTTTGCCGGGAAACGACATGGAATGGTACTTTTACAGCACAAG GGATAAGAAGTATCCAAATGGCTCTAGGACGAACCGTGCGACCCGAGCGGGTTACTGGAAGGCCACGGGGAAAGATCGTACAGTAGAATCAAAGAAGATG AAGATGGGAATGAAGAAGACACTGGTTTATTATAGAGGAAGGGCTCCTCATGGCCTTCGTACTAATTGGGTCATGCATGAATATCGTCTCACGCACGCTC CTTCCTCCTCCTTGAAGGAGTCGTATGCATTGTGCCGAGTGTTTAAGAAGAACATACAAATTCCAAAGAGAAAAGGGGAAGAAGAAGAAGCAGAAGAAGA GAGCACTAGTGTAGGAAAAGAAGAGGAAGAAGAAAAGGAGAAGAAGTGGAGAAAATGTGATGGTAATTATATTGAAGACGAGAGCTTGAAAAGAGCATCC GCGGAGACATCTTCATCAGAGCTAACTCAAGGGGTCCTTTTAGACGAAGCAAACAGCTCATCCATATTTGCTCTTCATTTCTCATCTTCTCTTCTGGACG ATCATGATCATCTTTTCTCAAACTATTCTCATCAGCTTCCATATCATCCTCCTCTTCAACTCCAAGATTTCCCTCAACTTTCTATGAACGAAGCAGAGAT TATGTCAATCCAACAAGACTTTCAATGCAGAGACTCTATGAACGGGACACTTGACGAAATCTTCTCTTCTTCCGCCACTTTCCCCGCTTCCCTT >IE_03E07_translated MSLPPGFRFHPTDEELVAYYLDRKVNGQAIELEIIPEVDLYKCEPWDLPEKSFLPGNDMEWYFYSTRDKKYPNGSRTNRATRAGYWKATGKDRTVESKKM KMGMKKTLVYYRGRAPHGLRTNWVMHEYRLTHAPSSSLKESYALCRVFKKNIQIPKRKGEEEEAEEESTSVGKEEEEEKEKKWRKCDGNYIEDESLKRAS AETSSSELTQGVLLDEANSSSIFALHFSSSLLDDHDHLFSNYSHQLPYHPPLQLQDFPQLSMNEAEIMSIQQDFQCRDSMNGTLDEIFSSSATFPASL
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 894/903 (99.0%)（アミノ酸配列で298/301 (99.0%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT1G54 ATGGCGCCCATGAGTTTGCCTCCAGGATTCAGGTTTCATCCCACTGATGAAGAACTGGTGGCTTACTATCTTGATAGGAAGGTCAACGGCCAAGCCATTG ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E ---------ATGAGTTTGCCTCCAGGATTCAGGTTTCATCCCACTGATGAAGAACTGGTGGCTTACTATCTTGATAGGAAGGTCAACGGCCAAGCCATTG 10 20 30 40 50 60 70 80 90 110 120 130 140 150 160 170 180 190 200 AT1G54 AGCTCGAGATCATCCCAGAAGTTGATCTTTATAAATGCGAGCCATGGGACTTGCCTGAAAAGTCATTTTTGCCGGGAAACGACATGGAATGGTACTTTTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E AGCTCGAGATCATCCCAGAAGTTGATCTTTATAAATGCGAGCCATGGGACTTGCCTGAAAAGTCATTTTTGCCGGGAAACGACATGGAATGGTACTTTTA 100 110 120 130 140 150 160 170 180 190 210 220 230 240 250 260 270 280 290 300 AT1G54 CAGCACAAGGGATAAGAAGTATCCAAATGGCTCTAGGACGAACCGTGCGACCCGAGCGGGTTACTGGAAGGCCACGGGGAAAGATCGTACAGTAGAATCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E CAGCACAAGGGATAAGAAGTATCCAAATGGCTCTAGGACGAACCGTGCGACCCGAGCGGGTTACTGGAAGGCCACGGGGAAAGATCGTACAGTAGAATCA 200 210 220 230 240 250 260 270 280 290 310 320 330 340 350 360 370 380 390 400 AT1G54 AAGAAGATGAAGATGGGAATGAAGAAGACACTGGTTTATTATAGAGGAAGGGCTCCTCATGGCCTTCGTACTAATTGGGTCATGCATGAATATCGTCTCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E AAGAAGATGAAGATGGGAATGAAGAAGACACTGGTTTATTATAGAGGAAGGGCTCCTCATGGCCTTCGTACTAATTGGGTCATGCATGAATATCGTCTCA 300 310 320 330 340 350 360 370 380 390 410 420 430 440 450 460 470 480 490 500 AT1G54 CGCACGCTCCTTCCTCCTCCTTGAAGGAGTCGTATGCATTGTGCCGAGTGTTTAAGAAGAACATACAAATTCCAAAGAGAAAAGGGGAAGAAGAAGAAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E CGCACGCTCCTTCCTCCTCCTTGAAGGAGTCGTATGCATTGTGCCGAGTGTTTAAGAAGAACATACAAATTCCAAAGAGAAAAGGGGAAGAAGAAGAAGC 400 410 420 430 440 450 460 470 480 490 510 520 530 540 550 560 570 580 590 600 AT1G54 AGAAGAAGAGAGCACTAGTGTAGGAAAAGAAGAGGAAGAAGAAAAGGAGAAGAAGTGGAGAAAATGTGATGGTAATTATATTGAAGACGAGAGCTTGAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E AGAAGAAGAGAGCACTAGTGTAGGAAAAGAAGAGGAAGAAGAAAAGGAGAAGAAGTGGAGAAAATGTGATGGTAATTATATTGAAGACGAGAGCTTGAAA 500 510 520 530 540 550 560 570 580 590 610 620 630 640 650 660 670 680 690 700 AT1G54 AGAGCATCCGCGGAGACATCTTCATCAGAGCTAACTCAAGGGGTCCTTTTAGACGAAGCAAACAGCTCATCCATATTTGCTCTTCATTTCTCATCTTCTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E AGAGCATCCGCGGAGACATCTTCATCAGAGCTAACTCAAGGGGTCCTTTTAGACGAAGCAAACAGCTCATCCATATTTGCTCTTCATTTCTCATCTTCTC 600 610 620 630 640 650 660 670 680 690 710 720 730 740 750 760 770 780 790 800 AT1G54 TTCTGGACGATCATGATCATCTTTTCTCAAACTATTCTCATCAGCTTCCATATCATCCTCCTCTTCAACTCCAAGATTTCCCTCAACTTTCTATGAACGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E TTCTGGACGATCATGATCATCTTTTCTCAAACTATTCTCATCAGCTTCCATATCATCCTCCTCTTCAACTCCAAGATTTCCCTCAACTTTCTATGAACGA 700 710 720 730 740 750 760 770 780 790 810 820 830 840 850 860 870 880 890 900 AT1G54 AGCAGAGATTATGTCAATCCAACAAGACTTTCAATGCAGAGACTCTATGAACGGGACACTTGACGAAATCTTCTCTTCTTCCGCCACTTTCCCCGCTTCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E AGCAGAGATTATGTCAATCCAACAAGACTTTCAATGCAGAGACTCTATGAACGGGACACTTGACGAAATCTTCTCTTCTTCCGCCACTTTCCCCGCTTCC 800 810 820 830 840 850 860 870 880 890 AT1G54 CTT ::: IE_03E CTT 10 20 30 40 50 60 70 80 90 100 AT1G54 MAPMSLPPGFRFHPTDEELVAYYLDRKVNGQAIELEIIPEVDLYKCEPWDLPEKSFLPGNDMEWYFYSTRDKKYPNGSRTNRATRAGYWKATGKDRTVES ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E ---MSLPPGFRFHPTDEELVAYYLDRKVNGQAIELEIIPEVDLYKCEPWDLPEKSFLPGNDMEWYFYSTRDKKYPNGSRTNRATRAGYWKATGKDRTVES 10 20 30 40 50 60 70 80 90 110 120 130 140 150 160 170 180 190 200 AT1G54 KKMKMGMKKTLVYYRGRAPHGLRTNWVMHEYRLTHAPSSSLKESYALCRVFKKNIQIPKRKGEEEEAEEESTSVGKEEEEEKEKKWRKCDGNYIEDESLK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E KKMKMGMKKTLVYYRGRAPHGLRTNWVMHEYRLTHAPSSSLKESYALCRVFKKNIQIPKRKGEEEEAEEESTSVGKEEEEEKEKKWRKCDGNYIEDESLK 100 110 120 130 140 150 160 170 180 190 210 220 230 240 250 260 270 280 290 300 AT1G54 RASAETSSSELTQGVLLDEANSSSIFALHFSSSLLDDHDHLFSNYSHQLPYHPPLQLQDFPQLSMNEAEIMSIQQDFQCRDSMNGTLDEIFSSSATFPAS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E RASAETSSSELTQGVLLDEANSSSIFALHFSSSLLDDHDHLFSNYSHQLPYHPPLQLQDFPQLSMNEAEIMSIQQDFQCRDSMNGTLDEIFSSSATFPAS 200 210 220 230 240 250 260 270 280 290 AT1G54 L : IE_03E L

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