◆◆◆IE_03E12 detail◆◆◆

Code name	IE_03E12
Locus	AT5G15150
Template	IT_03E12 (HR3371)
Donor Vector	pDONR207
Forward primer	IPF_03E12 (ggggacaagtttgtacaaaaaagcaggcttcATGGCCTTTCCTCAACATGGCTTCATGT)
Reverse primer	IPR_03E12 (ggggaccactttgtacaagaaagctgggttATTGAACTGATGATGATTGTGATGTTGTTG)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列93.30%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_03E12_879bp ATGGCCTTTCCTCAACATGGCTTCATGTTTCAGCAACTCCATGAAGACAATGCTCATCACTTACCTTCCCCAACCTCTCTCCCCTCTTGTCCTCCTCATC TCTTCTATGGAGGAGGAGGAAACTACATGATGAACAGATCAATGTCGTTCACGGGAGTATCAGATCATCATCATCTAACTCAAAAAAGCCCGACAACAAC GAACAACATGAATGATCAAGACCAGGTAGGCGAGGAAGACAACCTATCAGACGATGGGTCACATATGATGTTAGGAGAGAAGAAGAAGAGGCTGAATTTA GAGCAAGTTAGGGCATTAGAGAAGAGCTTCGAGCTAGGGAACAAGCTGGAGCCAGAGAGGAAGATGCAGCTTGCTAAGGCTTTAGGATTGCAGCCTAGGC AGATTGCAATATGGTTTCAGAACAGGAGAGCTAGGTGGAAGACAAAGCAGCTCGAGAGAGACTATGATTCTCTCAAAAAACAGTTCGATGTTTTGAAATC GGACAATGATTCTCTTCTTGCCCACAACAAGAAACTACATGCTGAGTTAGTGGCATTGAAGAAACACGACCGTAAAGAATCAGCAAAGATCAAGAGAGAA TTCGCAGAAGCTTCATGGAGCAACAATGGAAGCACAGAAAATAATCATAACAATAATAGTTCAGACGCTAATCATGTGAGCATGATCAAAGATCTTTTCC CTTCTTCGATCCGATCCGCGACCGCGACCACGACCTCTACCCATATTGATCATCAGATTGTTCAAGATCAAGATCAAGGGTTTTGCAATATGTTCAATGG TATTGATGAAACGACGTCGGCTAGTTATTGGGCATGGCCTGACCAGCAGCAACAACATCACAATCATCATCAGTTCAAT >IE_03E12_translated MAFPQHGFMFQQLHEDNAHHLPSPTSLPSCPPHLFYGGGGNYMMNRSMSFTGVSDHHHLTQKSPTTTNNMNDQDQVGEEDNLSDDGSHMMLGEKKKRLNL EQVRALEKSFELGNKLEPERKMQLAKALGLQPRQIAIWFQNRRARWKTKQLERDYDSLKKQFDVLKSDNDSLLAHNKKLHAELVALKKHDRKESAKIKRE FAEASWSNNGSTENNHNNNSSDANHVSMIKDLFPSSIRSATATTTSTHIDHQIVQDQDQGFCNMFNGIDETTSASYWAWPDQQQQHHNHHQFN
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 879/942 (93.3%)（アミノ酸配列で293/314 (93.3%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G15 ATGTATATGTATGAGGAGGAAAGGAACAATATCAATAATAATCAAGAAGGACTGAGATTGGAGATGGCCTTTCCTCAACATGGCTTCATGTTTCAGCAAC ::::::::::::::::::::::::::::::::::::: IE_03E ---------------------------------------------------------------ATGGCCTTTCCTCAACATGGCTTCATGTTTCAGCAAC 10 20 30 110 120 130 140 150 160 170 180 190 200 AT5G15 TCCATGAAGACAATGCTCATCACTTACCTTCCCCAACCTCTCTCCCCTCTTGTCCTCCTCATCTCTTCTATGGAGGAGGAGGAAACTACATGATGAACAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E TCCATGAAGACAATGCTCATCACTTACCTTCCCCAACCTCTCTCCCCTCTTGTCCTCCTCATCTCTTCTATGGAGGAGGAGGAAACTACATGATGAACAG 40 50 60 70 80 90 100 110 120 130 210 220 230 240 250 260 270 280 290 300 AT5G15 ATCAATGTCGTTCACGGGAGTATCAGATCATCATCATCTAACTCAAAAAAGCCCGACAACAACGAACAACATGAATGATCAAGACCAGGTAGGCGAGGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E ATCAATGTCGTTCACGGGAGTATCAGATCATCATCATCTAACTCAAAAAAGCCCGACAACAACGAACAACATGAATGATCAAGACCAGGTAGGCGAGGAA 140 150 160 170 180 190 200 210 220 230 310 320 330 340 350 360 370 380 390 400 AT5G15 GACAACCTATCAGACGATGGGTCACATATGATGTTAGGAGAGAAGAAGAAGAGGCTGAATTTAGAGCAAGTTAGGGCATTAGAGAAGAGCTTCGAGCTAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E GACAACCTATCAGACGATGGGTCACATATGATGTTAGGAGAGAAGAAGAAGAGGCTGAATTTAGAGCAAGTTAGGGCATTAGAGAAGAGCTTCGAGCTAG 240 250 260 270 280 290 300 310 320 330 410 420 430 440 450 460 470 480 490 500 AT5G15 GGAACAAGCTGGAGCCAGAGAGGAAGATGCAGCTTGCTAAGGCTTTAGGATTGCAGCCTAGGCAGATTGCAATATGGTTTCAGAACAGGAGAGCTAGGTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E GGAACAAGCTGGAGCCAGAGAGGAAGATGCAGCTTGCTAAGGCTTTAGGATTGCAGCCTAGGCAGATTGCAATATGGTTTCAGAACAGGAGAGCTAGGTG 340 350 360 370 380 390 400 410 420 430 510 520 530 540 550 560 570 580 590 600 AT5G15 GAAGACAAAGCAGCTCGAGAGAGACTATGATTCTCTCAAAAAACAGTTCGATGTTTTGAAATCGGACAATGATTCTCTTCTTGCCCACAACAAGAAACTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E GAAGACAAAGCAGCTCGAGAGAGACTATGATTCTCTCAAAAAACAGTTCGATGTTTTGAAATCGGACAATGATTCTCTTCTTGCCCACAACAAGAAACTA 440 450 460 470 480 490 500 510 520 530 610 620 630 640 650 660 670 680 690 700 AT5G15 CATGCTGAGTTAGTGGCATTGAAGAAACACGACCGTAAAGAATCAGCAAAGATCAAGAGAGAATTCGCAGAAGCTTCATGGAGCAACAATGGAAGCACAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E CATGCTGAGTTAGTGGCATTGAAGAAACACGACCGTAAAGAATCAGCAAAGATCAAGAGAGAATTCGCAGAAGCTTCATGGAGCAACAATGGAAGCACAG 540 550 560 570 580 590 600 610 620 630 710 720 730 740 750 760 770 780 790 800 AT5G15 AAAATAATCATAACAATAATAGTTCAGACGCTAATCATGTGAGCATGATCAAAGATCTTTTCCCTTCTTCGATCCGATCCGCGACCGCGACCACGACCTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E AAAATAATCATAACAATAATAGTTCAGACGCTAATCATGTGAGCATGATCAAAGATCTTTTCCCTTCTTCGATCCGATCCGCGACCGCGACCACGACCTC 640 650 660 670 680 690 700 710 720 730 810 820 830 840 850 860 870 880 890 900 AT5G15 TACCCATATTGATCATCAGATTGTTCAAGATCAAGATCAAGGGTTTTGCAATATGTTCAATGGTATTGATGAAACGACGTCGGCTAGTTATTGGGCATGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E TACCCATATTGATCATCAGATTGTTCAAGATCAAGATCAAGGGTTTTGCAATATGTTCAATGGTATTGATGAAACGACGTCGGCTAGTTATTGGGCATGG 740 750 760 770 780 790 800 810 820 830 910 920 930 940 AT5G15 CCTGACCAGCAGCAACAACATCACAATCATCATCAGTTCAAT :::::::::::::::::::::::::::::::::::::::::: IE_03E CCTGACCAGCAGCAACAACATCACAATCATCATCAGTTCAAT 840 850 860 870 10 20 30 40 50 60 70 80 90 100 AT5G15 MYMYEEERNNINNNQEGLRLEMAFPQHGFMFQQLHEDNAHHLPSPTSLPSCPPHLFYGGGGNYMMNRSMSFTGVSDHHHLTQKSPTTTNNMNDQDQVGEE ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E ---------------------MAFPQHGFMFQQLHEDNAHHLPSPTSLPSCPPHLFYGGGGNYMMNRSMSFTGVSDHHHLTQKSPTTTNNMNDQDQVGEE 10 20 30 40 50 60 70 110 120 130 140 150 160 170 180 190 200 AT5G15 DNLSDDGSHMMLGEKKKRLNLEQVRALEKSFELGNKLEPERKMQLAKALGLQPRQIAIWFQNRRARWKTKQLERDYDSLKKQFDVLKSDNDSLLAHNKKL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E DNLSDDGSHMMLGEKKKRLNLEQVRALEKSFELGNKLEPERKMQLAKALGLQPRQIAIWFQNRRARWKTKQLERDYDSLKKQFDVLKSDNDSLLAHNKKL 80 90 100 110 120 130 140 150 160 170 210 220 230 240 250 260 270 280 290 300 AT5G15 HAELVALKKHDRKESAKIKREFAEASWSNNGSTENNHNNNSSDANHVSMIKDLFPSSIRSATATTTSTHIDHQIVQDQDQGFCNMFNGIDETTSASYWAW :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03E HAELVALKKHDRKESAKIKREFAEASWSNNGSTENNHNNNSSDANHVSMIKDLFPSSIRSATATTTSTHIDHQIVQDQDQGFCNMFNGIDETTSASYWAW 180 190 200 210 220 230 240 250 260 270 310 AT5G15 PDQQQQHHNHHQFN :::::::::::::: IE_03E PDQQQQHHNHHQFN 280 290

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