◆◆◆IE_03F01 detail◆◆◆

Code name	IE_03F01
Locus	AT2G42380
Template	IT_03F01 (HR3400)
Donor Vector	pDONR207
Forward primer	IPF_03F01 (ggggacaagtttgtacaaaaaagcaggcttcATGACACAACATTGGCCTGATTTCTCTT)
Reverse primer	IPR_03F01 (ggggaccactttgtacaagaaagctgggttTGAGACATTGAGGAGCTGCTCTGTTTCAAC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列93.10%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_03F01_933bp ATGACACAACATTGGCCTGATTTCTCTTCCCAAAAGCTCTCTCCTTTCTCTACCCCAACCGCAACCGCTGTCGCCACCGCTACAACCACCGTACAAAACC CCTCATGGGTCGACGAATTCCTCGACTTCTCAGCGTCTCGCCGTGGCAACCACCGTCGTTCCATCAGCGACTCTATCGCATTCCTCGAAGCTCCAACAGT CAGCATCGAAGACCACCAATTCGACAGGTTCGATGACGAACAGTTCATGTCGATGTTCACCGACGACGACAACCTTCATAGCAATCCTTCCCATATCAAC AACAAAAATAACAATGTGGGGCCCACGGGATCTTCCTCGAACACATCCACGCCGTCCAATAGCTTCAACGACGATAACAAAGAATTACCACCGTCCGATC ATAACATGAACAATAATATCAACAACAACTATAACGATGAAGTCCAAAGCCAATGCAAGATGGAGCCAGAAGATGGTACGGCGTCGAATAACAATTCCGG TGATAGCTCCGGCAACCGGATTCTCGATCCCAAAAGGGTTAAGAGAATATTAGCAAATCGGCAATCAGCACAGAGATCAAGGGTGAGGAAACTGCAATAC ATATCAGAGCTCGAACGTAGCGTCACTTCGTTGCAGGCGGAAGTGTCAGTGTTATCGCCAAGAGTTGCATTCTTGGATCATCAACGTTTGCTTCTTAACG TTGACAACAGCGCTCTCAAGCAACGAATCGCTGCTTTATCTCAAGACAAGCTTTTCAAAGACGCACATCAAGAAGCATTGAAGAGAGAAATAGAGAGACT TCGACAAGTGTATAATCAACAAAGCCTCACGAATGTGGAAAATGCAAATCATTTATCGGCGACCGGAGCCGGTGCTACTCCGGCCGTCGACATCAAGTCG TCCGTTGAAACAGAGCAGCTCCTCAATGTCTCA >IE_03F01_translated MTQHWPDFSSQKLSPFSTPTATAVATATTTVQNPSWVDEFLDFSASRRGNHRRSISDSIAFLEAPTVSIEDHQFDRFDDEQFMSMFTDDDNLHSNPSHIN NKNNNVGPTGSSSNTSTPSNSFNDDNKELPPSDHNMNNNINNNYNDEVQSQCKMEPEDGTASNNNSGDSSGNRILDPKRVKRILANRQSAQRSRVRKLQY ISELERSVTSLQAEVSVLSPRVAFLDHQRLLLNVDNSALKQRIAALSQDKLFKDAHQEALKREIERLRQVYNQQSLTNVENANHLSATGAGATPAVDIKS SVETEQLLNVS
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 900/963 (93.5%)（アミノ酸配列で299/321 (93.1%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT2G42 ATGGCACAACTCCCTCCTAAAATCCCCAACATGACACAACATTGGCCTGATTTCTCTTCCCAAAAGCTCTCTCCTTTCTCTACCCCAACCGCAACCGCTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03F ------------------------------ATGACACAACATTGGCCTGATTTCTCTTCCCAAAAGCTCTCTCCTTTCTCTACCCCAACCGCAACCGCTG 10 20 30 40 50 60 70 110 120 130 140 150 160 170 180 190 200 AT2G42 TCGCCACCGCTACAACCACCGTACAAAACCCCTCATGGGTCGACGAATTCCTCGACTTCTCAGCGTCTCGCCGTGGCAACCACCGTCGTTCCATCAGCGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03F TCGCCACCGCTACAACCACCGTACAAAACCCCTCATGGGTCGACGAATTCCTCGACTTCTCAGCGTCTCGCCGTGGCAACCACCGTCGTTCCATCAGCGA 80 90 100 110 120 130 140 150 160 170 210 220 230 240 250 260 270 280 290 300 AT2G42 CTCTATCGCATTCCTCGAAGCTCCAACAGTCAGCATCGAAGACCACCAATTCGACAGGTTCGATGACGAACAGTTCATGTCGATGTTCACCGACGACGAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03F CTCTATCGCATTCCTCGAAGCTCCAACAGTCAGCATCGAAGACCACCAATTCGACAGGTTCGATGACGAACAGTTCATGTCGATGTTCACCGACGACGAC 180 190 200 210 220 230 240 250 260 270 310 320 330 340 350 360 370 380 390 400 AT2G42 AACCTTCATAGCAATCCTTCCCATATCAACAACAAAAATAACAATGTGGGGCCCACGGGATCTTCCTCGAACACATCCACGCCGTCCAATAGCTTCAACG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03F AACCTTCATAGCAATCCTTCCCATATCAACAACAAAAATAACAATGTGGGGCCCACGGGATCTTCCTCGAACACATCCACGCCGTCCAATAGCTTCAACG 280 290 300 310 320 330 340 350 360 370 410 420 430 440 450 460 470 480 490 500 AT2G42 ACGATAACAAAGAATTACCACCGTCCGATCATAACATGAACAATAATATCAACAACAACTATAACGATGAAGTCCAAAGCCAATGCAAGATGGAGCCAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03F ACGATAACAAAGAATTACCACCGTCCGATCATAACATGAACAATAATATCAACAACAACTATAACGATGAAGTCCAAAGCCAATGCAAGATGGAGCCAGA 380 390 400 410 420 430 440 450 460 470 510 520 530 540 550 560 570 580 590 600 AT2G42 AGATGGTACGGCGTCGAATAACAATTCCGGTGATAGCTCCGGCAACCGGATTCTCGATCCCAAAAGGGTTAAGAGAATATTAGCAAATCGGCAATCAGCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03F AGATGGTACGGCGTCGAATAACAATTCCGGTGATAGCTCCGGCAACCGGATTCTCGATCCCAAAAGGGTTAAGAGAATATTAGCAAATCGGCAATCAGCA 480 490 500 510 520 530 540 550 560 570 610 620 630 640 650 660 AT2G42 CAGAGATCAAGGGTGAGGAAACTGCAATACATATCAGAGCTCGAAC---------------------------------TGTTATCGCCAAGAGTTGCAT :::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::: IE_03F CAGAGATCAAGGGTGAGGAAACTGCAATACATATCAGAGCTCGAACGTAGCGTCACTTCGTTGCAGGCGGAAGTGTCAGTGTTATCGCCAAGAGTTGCAT 580 590 600 610 620 630 640 650 660 670 670 680 690 700 710 720 730 740 750 760 AT2G42 TCTTGGATCATCAACGTTTGCTTCTTAACGTTGACAACAGCGCTCTCAAGCAACGAATCGCTGCTTTATCTCAAGACAAGCTTTTCAAAGACGCACATCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03F TCTTGGATCATCAACGTTTGCTTCTTAACGTTGACAACAGCGCTCTCAAGCAACGAATCGCTGCTTTATCTCAAGACAAGCTTTTCAAAGACGCACATCA 680 690 700 710 720 730 740 750 760 770 770 780 790 800 810 820 830 840 850 860 AT2G42 AGAAGCATTGAAGAGAGAAATAGAGAGACTTCGACAAGTGTATAATCAACAAAGCCTCACGAATGTGGAAAATGCAAATCATTTATCGGCGACCGGAGCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03F AGAAGCATTGAAGAGAGAAATAGAGAGACTTCGACAAGTGTATAATCAACAAAGCCTCACGAATGTGGAAAATGCAAATCATTTATCGGCGACCGGAGCC 780 790 800 810 820 830 840 850 860 870 870 880 890 900 910 920 930 AT2G42 GGTGCTACTCCGGCCGTCGACATCAAGTCGTCCGTTGAAACAGAGCAGCTCCTCAATGTCTCA ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03F GGTGCTACTCCGGCCGTCGACATCAAGTCGTCCGTTGAAACAGAGCAGCTCCTCAATGTCTCA 880 890 900 910 920 930 10 20 30 40 50 60 70 80 90 100 AT2G42 MAQLPPKIPNMTQHWPDFSSQKLSPFSTPTATAVATATTTVQNPSWVDEFLDFSASRRGNHRRSISDSIAFLEAPTVSIEDHQFDRFDDEQFMSMFTDDD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03F ----------MTQHWPDFSSQKLSPFSTPTATAVATATTTVQNPSWVDEFLDFSASRRGNHRRSISDSIAFLEAPTVSIEDHQFDRFDDEQFMSMFTDDD 10 20 30 40 50 60 70 80 90 110 120 130 140 150 160 170 180 190 200 AT2G42 NLHSNPSHINNKNNNVGPTGSSSNTSTPSNSFNDDNKELPPSDHNMNNNINNNYNDEVQSQCKMEPEDGTASNNNSGDSSGNRILDPKRVKRILANRQSA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03F NLHSNPSHINNKNNNVGPTGSSSNTSTPSNSFNDDNKELPPSDHNMNNNINNNYNDEVQSQCKMEPEDGTASNNNSGDSSGNRILDPKRVKRILANRQSA 100 110 120 130 140 150 160 170 180 190 210 220 230 240 250 260 270 280 AT2G42 QRSRVRKLQYISELE-----------LLSPRVAFLDHQRLLLNVDNSALKQRIAALSQDKLFKDAHQEALKREIERLRQVYNQQSLTNVENANHLSATGA ::::::::::::::: .::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03F QRSRVRKLQYISELERSVTSLQAEVSVLSPRVAFLDHQRLLLNVDNSALKQRIAALSQDKLFKDAHQEALKREIERLRQVYNQQSLTNVENANHLSATGA 200 210 220 230 240 250 260 270 280 290 290 300 310 AT2G42 GATPAVDIKSSVETEQLLNVS ::::::::::::::::::::: IE_03F GATPAVDIKSSVETEQLLNVS 300 310

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