◆◆◆IE_03F08（IE_09A08） detail◆◆◆

Code nameIE_03F08（IE_09A08）
LocusAT4G22810
TemplateIT_03F08 (HR3542)
Donor VectorpDONR207
Forward primerIPF_03F08 (ggggacaagtttgtacaaaaaagcaggcttcATGGATCCAGTACAATCTCATGGATCAC)
Reverse primerIPR_03F08 (ggggaccactttgtacaagaaagctgggttATACGGTGGTCGTCCCGTTGACCAATAAGA)
Alignment with template seq.1st配列解析が行えなかったサンプルです。2ndアミノ酸配列100%一致
Alignment with TAIR7CDS-アミノ酸配列100%一致
comment1st:(PCRで目的サイズの増幅が確認できたが、コロニーが得られなかった)→2nd(09A08):100%
sequence 
>IE_03F08_972bp
ATGGATCCAGTACAATCTCATGGATCACAAAGCTCTCTACCTCCTCCTTTCCACGCAAGAGACTTTCAATTACATCTTCAACAACAGCAACAAGAGTTCT
TCCTCCACCATCACCAGCAACAAAGAAACCAAACCGATGGTGACCAACAAGGAGGATCAGGAGGAAACCGACAAATCAAGATGGATCGTGAAGAGACAAG
CGACAACATAGACAACATAGCTAACAACAGCGGTAGTGAAGGTAAAGACATAGATATACACGGTGGTTCAGGAGAAGGAGGTGGTGGCTCCGGAGGAGAT
CATCAGATGACAAGAAGACCAAGAGGAAGACCAGCGGGATCCAAGAACAAACCAAAACCACCGATTATCATCACACGGGACAGCGCAAACGCGCTTAGAA
CCCACGTGATGGAGATCGGAGATGGCTGCGACTTAGTCGAAAGCGTTGCCACTTTTGCACGAAGACGCCAACGCGGCGTTTGCGTTATGAGCGGTACTGG
AAATGTTACTAACGTCACTATACGTCAGCCTGGATCTCATCCTTCTCCTGGCTCGGTAGTTAGTCTTCACGGAAGGTTCGAGATTCTATCTCTCTCAGGA
TCTTTTCTCCCTCCTCCGGCTCCTCCTACAGCCACCGGATTGAGTGTTTACCTCGCTGGAGGACAAGGACAGGTGGTTGGAGGAAGCGTAGTTGGTCCGT
TGTTATGTGCTGGTCCTGTCGTTGTCATGGCTGCGTCTTTTAGCAATGCGGCGTACGAAAGGTTGCCTTTAGAGGAAGATGAGATGCAGACGCCGGTTCA
TGGCGGAGGAGGAGGAGGATCATTGGAGTCGCCGCCAATGATGGGACAACAACTGCAACATCAGCAACAAGCTATGTCAGGTCATCAAGGGTTACCACCT
AATCTTCTTGGTTCGGTTCAGTTGCAGCAGCAACATGATCAGTCTTATTGGTCAACGGGACGACCACCGTAT

>IE_03F08_translated
MDPVQSHGSQSSLPPPFHARDFQLHLQQQQQEFFLHHHQQQRNQTDGDQQGGSGGNRQIKMDREETSDNIDNIANNSGSEGKDIDIHGGSGEGGGGSGGD
HQMTRRPRGRPAGSKNKPKPPIIITRDSANALRTHVMEIGDGCDLVESVATFARRRQRGVCVMSGTGNVTNVTIRQPGSHPSPGSVVSLHGRFEILSLSG
SFLPPPAPPTATGLSVYLAGGQGQVVGGSVVGPLLCAGPVVVMAASFSNAAYERLPLEEDEMQTPVHGGGGGGSLESPPMMGQQLQHQQQAMSGHQGLPP
NLLGSVQLQQQHDQSYWSTGRPPY
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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