◆◆◆IE_03F10 detail◆◆◆

Code nameIE_03F10
LocusAT1G60380
TemplateIT_03F10 (HR3557)
Donor VectorpDONR207
Forward primerIPF_03F10 (ggggacaagtttgtacaaaaaagcaggcttcATGGCGGACACACTGCTCAACGCAGAAG)
Reverse primerIPR_03F10 (ggggaccactttgtacaagaaagctgggttGCTAGTTATCCTCGGTAAACACTTTACCTT)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_03F10_954bp
ATGGCGGACACACTGCTCAACGCAGAAGACGAAGTAATAATCTCACGTTATCTGAAGCCTATGATCGTTAACAGAGTATCATGGCCTGATCTCTTCATCG
AAGACGCAGACGTGTTCAACAAGGATCCATATGTGAAGTTCCATGCTGAGATCCCTAGCTTCGTGATCGTTAAACCACGAACAAAGGCTTGTGGTAAAAC
CGATGGATGTGATTCGGGTTGCTGGAGGATCATTGGTCGTGATAAGCTGATAAAGTCGGAGGAGACTGGTAAGATTCTAGGGTTCAAGAAGATACTCAAG
TTCTGCCTAAAGTGGAAACCTAGAGAATACAAGAGAAGTTTGGTAATGGAAGAGTATAGGCTTACCAATAACTTCAACTGGAAGCAAGATCATGTGATTT
GCAAGATTCGGCTTTTGTTTGAAGCAGAAATTAGTTTCTTGCTAGCCAAGCATTTCTACACTACATCAGACTCACTTCCTCGAAATGTGCTGTTGCCAGC
TTATGGATTCTGTTCACCAGATAAACAAGAGGAGGACGAATTTTATCCGGTGACGATAATGATTTCAGAAGGAAAAGATTGGCCTAGCTACGTTACCAAC
AACGTGTATTGTCTGCATCCATCGGAGCTTGTGAATGTTCACGATGGGAAGTTTCATGATAACGGAATCTGCATCTTCGCTAACAGGACTTGTGGTGTAA
CCGATAAATGCAATGAAGGTTACTGGAAGATTAAGCACCGTGAGAAGCTGATCATGTCACGGTACGGGCAGACCATTGGTTGGAAGAAAGTTTTTCAGTT
TTATGAAACGGAGAAAGAAAGACATTTTGGTAATGGAGAAGAAGTGAAGGTAACTTGGACTCTAAAAGAGTATAGGCTTACCAGAAAAATGAACAAGAAT
AAAGTGGTGTGCGTTATCAAGTATAAGGTAAAGTGTTTACCGAGGATAACTAGC

>IE_03F10_translated
MADTLLNAEDEVIISRYLKPMIVNRVSWPDLFIEDADVFNKDPYVKFHAEIPSFVIVKPRTKACGKTDGCDSGCWRIIGRDKLIKSEETGKILGFKKILK
FCLKWKPREYKRSLVMEEYRLTNNFNWKQDHVICKIRLLFEAEISFLLAKHFYTTSDSLPRNVLLPAYGFCSPDKQEEDEFYPVTIMISEGKDWPSYVTN
NVYCLHPSELVNVHDGKFHDNGICIFANRTCGVTDKCNEGYWKIKHREKLIMSRYGQTIGWKKVFQFYETEKERHFGNGEEVKVTWTLKEYRLTRKMNKN
KVVCVIKYKVKCLPRITS
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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