| Code name | IE_03F11 | |||
|---|---|---|---|---|
| Locus | AT2G43000 | |||
| Template | IT_03F11 (HR3559) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_03F11 (ggggacaagtttgtacaaaaaagcaggcttcATGAGTGGCGAAGGTAACTTAGGTAAGG) | |||
| Reverse primer | IPR_03F11 (ggggaccactttgtacaagaaagctgggttGGGTTTAGTGTTGCCATCTATAACAGATCT) | |||
| Alignment with template seq. | 1st | 配列解析が行えなかったサンプルです。 | 2nd | アミノ酸配列100%一致 |
| Alignment with TAIR7CDS | - | アミノ酸配列100%一致 | ||
| comment | 1st:(Sequence解析まで行ったが、読み直す必要がある?) | |||
| sequence |
>IE_03F11_825bp ATGAGTGGCGAAGGTAACTTAGGTAAGGATCATGAAGAAGAAAACGAAGCACCACTTCCTGGGTTCAGGTTTCATCCGACGGATGAAGAGCTTTTAGGAT ACTATCTTCGAAGAAAAGTAGAGAACAAAACCATCAAACTCGAACTTATCAAACAGATCGATATCTATAAGTACGATCCTTGGGATCTTCCAAGAGTGAG CAGCGTCGGAGAAAAGGAGTGGTACTTCTTCTGCATGAGAGGTAGGAAATACAGGAATAGCGTTCGACCAAACCGAGTGACCGGTTCAGGTTTCTGGAAA GCCACTGGTATTGATAAACCGGTTTACTCCAATCTTGACTGTGTTGGTCTCAAGAAATCTCTGGTTTACTATCTTGGTTCAGCCGGTAAAGGCACCAAAA CCGATTGGATGATGCATGAATTCCGCCTCCCCTCCACCACGAAAACCGACTCTCCAGCTCAACAAGCAGAGGTATGGACACTTTGCAGAATCTTCAAACG AGTCACATCTCAAAGAAACCCAACCATCTTACCACCAAACCGAAAACCGGTTATCACTTTAACCGACACTTGTTCTAAGACCAGCAGCTTAGATTCCGAC CACACGAGCCACCGTACAGTAGATTCCATGTCCCACGAGCCGCCGCTTCCACAGCCACAGAATCCTTATTGGAACCAACATATAGTTGGTTTTAATCAAC CGACATATACTGGTAATGATAATAACCTCCTGATGAGTTTCTGGAACGGCAACGGTGGAGATTTCATAGGAGACTCAGCAAGTTGGGATGAACTTAGATC TGTTATAGATGGCAACACTAAACCC >IE_03F11_translated MSGEGNLGKDHEEENEAPLPGFRFHPTDEELLGYYLRRKVENKTIKLELIKQIDIYKYDPWDLPRVSSVGEKEWYFFCMRGRKYRNSVRPNRVTGSGFWK ATGIDKPVYSNLDCVGLKKSLVYYLGSAGKGTKTDWMMHEFRLPSTTKTDSPAQQAEVWTLCRIFKRVTSQRNPTILPPNRKPVITLTDTCSKTSSLDSD HTSHRTVDSMSHEPPLPQPQNPYWNQHIVGFNQPTYTGNDNNLLMSFWNGNGGDFIGDSASWDELRSVIDGNTKP |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 100%一致しました。 |
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