◆◆◆IE_03G01 detail◆◆◆

Code name	IE_03G01
Locus	AT3G56530
Template	IT_03G01 (HR3563)
Donor Vector	pDONR207
Forward primer	IPF_03G01 (ggggacaagtttgtacaaaaaagcaggcttcATGGCGATTCCACAAAGAAACAAGCGCA)
Reverse primer	IPR_03G01 (ggggaccactttgtacaagaaagctgggttATGTTTTATCATTCCTTCTGTGTCAAAGAA)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.40%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_03G01_963bp ATGGCGATTCCACAAAGAAACAAGCGCAAAGCAAGATCCTCGCCGGAACGGCTAACACAACCGCCGGAACTACCACACAACTCCGACGTTCCTTCTTCTT CGTCTTCTTCTGCCGCTGATAATTTCTTTTCTTGGTCGACGAAACAGTTTGCTTTCCCGCCAGGTTATAGATTTGTACCAAAGGACCAAGAACTCATCTT TCATTACTTGAAACCCTTTTCGCAAGGCAATAAATGCTCATTGCTCAATGTTCCTATCCATCGTGTGAACATCTACGAGTCAAATCCACAACATCTTTCA GAGAAATATGAGAAGGGTAATGATAAAGATTGGTTCTATATATCGGAGAGGACCAAGACCGGTAAGGCTGGAAGAAGTAATAAACGTGTCGACAATGGAG GATATTGGAGCGCAACTGTAGCTGCCCAAAAGATCAACGCTGGAAATGGCATAGTCGGCTACAAGACTTCACTAGAGTACTATGTTGGGAAACAATCAAA TAGTGTCAAAGGGGATTGGTTAATGCAAGAGTACTGGTTTGAGTCTTCTGATGATAACAACAACGAGAAGGTGAGGGTGGATCATGCTTTGTGCAAGATT TATCTGACTCCAGCGGCAGCAAAGAAGAAGAAAGCTGAGGAAGCAGAGAATGAGAAGCTGAAGAAAGAAGAAGACGTTGAGCAGCTGGATTTGAATCAAC CAGACCAATTGCAGCTGCAACAGCCTCATGATATTGTGTATCAACCGCAGTATTGTTTACTTCCAGAACATCATCAGCCGCAGCCTTTTCCTGATAACTT TTCTGAGTTGATCAGCTTTCAACAGCAACCAGTGATGATCCCAGACGATTTTGAAGATTTCTTGGCTGAATTCACTAAACCACATTCACTGGATGGAGAT GAAGAATTCAACAATTATGGTTTGTTTGAAGGCTTCTTTGACACAGAAGGAATGATAAAACAT >IE_03G01_translated MAIPQRNKRKARSSPERLTQPPELPHNSDVPSSSSSSAADNFFSWSTKQFAFPPGYRFVPKDQELIFHYLKPFSQGNKCSLLNVPIHRVNIYESNPQHLS EKYEKGNDKDWFYISERTKTGKAGRSNKRVDNGGYWSATVAAQKINAGNGIVGYKTSLEYYVGKQSNSVKGDWLMQEYWFESSDDNNNEKVRVDHALCKI YLTPAAAKKKKAEEAENEKLKKEEDVEQLDLNQPDQLQLQQPHDIVYQPQYCLLPEHHQPQPFPDNFSELISFQQQPVMIPDDFEDFLAEFTKPHSLDGD EEFNNYGLFEGFFDTEGMIKH
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 957/963 (99.4%)（アミノ酸配列で319/321 (99.4%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT3G56 ATGGCGATTCCACAAAGAAACAAGCGCAAAGCAAGATCCTCGCCGGAACGGCTAACACAACCGCCGGAACTACCACACAACTCCGACGTTCCTTCTTCTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03G ATGGCGATTCCACAAAGAAACAAGCGCAAAGCAAGATCCTCGCCGGAACGGCTAACACAACCGCCGGAACTACCACACAACTCCGACGTTCCTTCTTCTT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT3G56 CGTCTTCTTCTGCCGCTGATAATTTCTTTTCTTGGTCGACGAAACAGTTTGCTTTCCCGCCAGGTTATAGATTTGTACCAAAGGACCAAGAACTCATCTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03G CGTCTTCTTCTGCCGCTGATAATTTCTTTTCTTGGTCGACGAAACAGTTTGCTTTCCCGCCAGGTTATAGATTTGTACCAAAGGACCAAGAACTCATCTT 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT3G56 TCATTACTTGAAACCCTTTTCGCAAGGCAATAAATGCTCATTGCTCAATGTTCCTATCCATCGTGTGAACATCTACGAGTCAAATCCACAACATCTTTCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03G TCATTACTTGAAACCCTTTTCGCAAGGCAATAAATGCTCATTGCTCAATGTTCCTATCCATCGTGTGAACATCTACGAGTCAAATCCACAACATCTTTCA 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT3G56 GAGAAATATGAGAAGGGTAATGATAAAGATTGGTTCTATATATCGGAGAGGACCAAGACCGGTAAGGCTGGAAGAAGTAATAAACGTGTCGACAATGGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03G GAGAAATATGAGAAGGGTAATGATAAAGATTGGTTCTATATATCGGAGAGGACCAAGACCGGTAAGGCTGGAAGAAGTAATAAACGTGTCGACAATGGAG 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT3G56 GATATTGGAGCGCAACTGTAGCTGCCCAAAAGATCAACGCTGGAAATGGCATAGTCGGCTACAAGACTTCACTAGAGTACTATGTTGGGAAACAATCAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03G GATATTGGAGCGCAACTGTAGCTGCCCAAAAGATCAACGCTGGAAATGGCATAGTCGGCTACAAGACTTCACTAGAGTACTATGTTGGGAAACAATCAAA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 AT3G56 TAGTGTCAAAGGGGATTGGTTAATGCAAGAGTACTGGTTTGAGTCTTCTGATGATAACAACAACGAGAA------GGTGGATCATGCTTTGTGCAAGATT ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::: IE_03G TAGTGTCAAAGGGGATTGGTTAATGCAAGAGTACTGGTTTGAGTCTTCTGATGATAACAACAACGAGAAGGTGAGGGTGGATCATGCTTTGTGCAAGATT 510 520 530 540 550 560 570 580 590 600 600 610 620 630 640 650 660 670 680 690 AT3G56 TATCTGACTCCAGCGGCAGCAAAGAAGAAGAAAGCTGAGGAAGCAGAGAATGAGAAGCTGAAGAAAGAAGAAGACGTTGAGCAGCTGGATTTGAATCAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03G TATCTGACTCCAGCGGCAGCAAAGAAGAAGAAAGCTGAGGAAGCAGAGAATGAGAAGCTGAAGAAAGAAGAAGACGTTGAGCAGCTGGATTTGAATCAAC 610 620 630 640 650 660 670 680 690 700 700 710 720 730 740 750 760 770 780 790 AT3G56 CAGACCAATTGCAGCTGCAACAGCCTCATGATATTGTGTATCAACCGCAGTATTGTTTACTTCCAGAACATCATCAGCCGCAGCCTTTTCCTGATAACTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03G CAGACCAATTGCAGCTGCAACAGCCTCATGATATTGTGTATCAACCGCAGTATTGTTTACTTCCAGAACATCATCAGCCGCAGCCTTTTCCTGATAACTT 710 720 730 740 750 760 770 780 790 800 800 810 820 830 840 850 860 870 880 890 AT3G56 TTCTGAGTTGATCAGCTTTCAACAGCAACCAGTGATGATCCCAGACGATTTTGAAGATTTCTTGGCTGAATTCACTAAACCACATTCACTGGATGGAGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03G TTCTGAGTTGATCAGCTTTCAACAGCAACCAGTGATGATCCCAGACGATTTTGAAGATTTCTTGGCTGAATTCACTAAACCACATTCACTGGATGGAGAT 810 820 830 840 850 860 870 880 890 900 900 910 920 930 940 950 AT3G56 GAAGAATTCAACAATTATGGTTTGTTTGAAGGCTTCTTTGACACAGAAGGAATGATAAAACAT ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03G GAAGAATTCAACAATTATGGTTTGTTTGAAGGCTTCTTTGACACAGAAGGAATGATAAAACAT 910 920 930 940 950 960 10 20 30 40 50 60 70 80 90 100 AT3G56 MAIPQRNKRKARSSPERLTQPPELPHNSDVPSSSSSSAADNFFSWSTKQFAFPPGYRFVPKDQELIFHYLKPFSQGNKCSLLNVPIHRVNIYESNPQHLS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03G MAIPQRNKRKARSSPERLTQPPELPHNSDVPSSSSSSAADNFFSWSTKQFAFPPGYRFVPKDQELIFHYLKPFSQGNKCSLLNVPIHRVNIYESNPQHLS 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 AT3G56 EKYEKGNDKDWFYISERTKTGKAGRSNKRVDNGGYWSATVAAQKINAGNGIVGYKTSLEYYVGKQSNSVKGDWLMQEYWFESSDDNNNEK--VDHALCKI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::: IE_03G EKYEKGNDKDWFYISERTKTGKAGRSNKRVDNGGYWSATVAAQKINAGNGIVGYKTSLEYYVGKQSNSVKGDWLMQEYWFESSDDNNNEKVRVDHALCKI 110 120 130 140 150 160 170 180 190 200 200 210 220 230 240 250 260 270 280 290 AT3G56 YLTPAAAKKKKAEEAENEKLKKEEDVEQLDLNQPDQLQLQQPHDIVYQPQYCLLPEHHQPQPFPDNFSELISFQQQPVMIPDDFEDFLAEFTKPHSLDGD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03G YLTPAAAKKKKAEEAENEKLKKEEDVEQLDLNQPDQLQLQQPHDIVYQPQYCLLPEHHQPQPFPDNFSELISFQQQPVMIPDDFEDFLAEFTKPHSLDGD 210 220 230 240 250 260 270 280 290 300 300 310 AT3G56 EEFNNYGLFEGFFDTEGMIKH ::::::::::::::::::::: IE_03G EEFNNYGLFEGFFDTEGMIKH 310 320

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