◆◆◆IE_03H01 detail◆◆◆

Code name	IE_03H01
Locus	AT4G01280
Template	IT_03H01 (HR3718)
Donor Vector	pDONR207
Forward primer	IPF_03H01 (ggggacaagtttgtacaaaaaagcaggcttcATGGTGTCCGTAAACCCTAGACCTAAGG)
Reverse primer	IPR_03H01 (ggggaccactttgtacaagaaagctgggttTTTCAAAGCTTTAGCGCTGTATGATGATAT)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.70%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_03H01_909bp ATGGTGTCCGTAAACCCTAGACCTAAGGGTTTTCCAGTTTTCGATTCCTCGAATATGAGTTTACCAAGCTCCGATGGATTTGGTTCGATTCCGGCCACGG GACGGACCAGTACGGTGTCGTTTTCTGAGGATCCGACGACGAAGATTCGGAAGCCGTACACAATCAAGAAGTCGAGAGAGAATTGGACAGATCAAGAGCA CGATAAATTTCTAGAAGCTCTTCACTTATTCGATAGGGATTGGAAGAAAATAGAAGCCTTTGTTGGATCAAAAACAGTAGTTCAGATACGAAGCCACGCT CAGAAATACTTTCTCAAAGTTCAGAAGAGTGGTGCTAACGAACATCTTCCACCTCCTCGACCTAAGAGGAAAGCGAGTCATCCTTATCCTATAAAGGCTC CTAAAAATGTTGCTTATACCTCTCTCCCGTCTTCGAGTACATTACCGTTGCTTGAGCCTGGTTATTTGTATAGCTCTGATTCGAAGTCATTGATGGGAAA CCAGGCTGTTTGTGCATCTACCTCTTCTTCGTGGAATCATGAATCGACAAATCTGCCAAAACCGGTGATTGAAGTAGAGGAACCGGGAGTCTCGGCCACG GCTCCTCTCCCAAATAATCGCTGCAGACAGGAAGATACAGAGAGGGTACGAGCAGTGACAAAGCCAAATAACGAAGAAAGTTGTGAAAAGCCACATAGAG TGATGCCGAATTTTGCTGAAGTTTACAGCTTCATTGGAAGTGTCTTCGATCCCAACACATCAGGCCACCTCCAGAGATTAAAGCAGATGGATCCAATAAA TATGGAAACGGTTCTTTTACTGATGCAAAACCTGTCTGTAAATCTGACAAGTCCCGAGTTTGCAGAGCAAAGGAGGTTGATATCATCATACAGCGCTAAA GCTTTGAAA >IE_03H01_translated MVSVNPRPKGFPVFDSSNMSLPSSDGFGSIPATGRTSTVSFSEDPTTKIRKPYTIKKSRENWTDQEHDKFLEALHLFDRDWKKIEAFVGSKTVVQIRSHA QKYFLKVQKSGANEHLPPPRPKRKASHPYPIKAPKNVAYTSLPSSSTLPLLEPGYLYSSDSKSLMGNQAVCASTSSSWNHESTNLPKPVIEVEEPGVSAT APLPNNRCRQEDTERVRAVTKPNNEESCEKPHRVMPNFAEVYSFIGSVFDPNTSGHLQRLKQMDPINMETVLLLMQNLSVNLTSPEFAEQRRLISSYSAK ALK
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 906/909 (99.7%)（アミノ酸配列で302/303 (99.7%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G01 ATGGTGTCCGTAAACCCTAGACCTAAGGGTTTTCCAGTTTTCGATTCCTCGAATATGAGTTTACCAAGCTCCGATGGATTTGGTTCGATTCCGGCCACGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H ATGGTGTCCGTAAACCCTAGACCTAAGGGTTTTCCAGTTTTCGATTCCTCGAATATGAGTTTACCAAGCTCCGATGGATTTGGTTCGATTCCGGCCACGG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G01 GACGGACCAGTACGGTGTCGTTTTCTGAGGATCCGACGACGAAGATTCGGAAGCCGTACACAATCAAGAAGTCGAGAGAGAATTGGACAGATCAAGAGCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H GACGGACCAGTACGGTGTCGTTTTCTGAGGATCCGACGACGAAGATTCGGAAGCCGTACACAATCAAGAAGTCGAGAGAGAATTGGACAGATCAAGAGCA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G01 CGATAAATTTCTAGAAGCTCTTCACTTATTCGATAGGGATTGGAAGAAAATAGAAGCCTTTGTTGGATCAAAAACAGTAGTTCAGATACGAAGCCACGCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H CGATAAATTTCTAGAAGCTCTTCACTTATTCGATAGGGATTGGAAGAAAATAGAAGCCTTTGTTGGATCAAAAACAGTAGTTCAGATACGAAGCCACGCT 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT4G01 CAGAAATACTTTCTCAAAGTTCAGAAGAGTGGTGCTAACGAACATCTTCCACCTCCTCGACCTAAGAGGAAAGCGAGTCATCCTTATCCTATAAAGGCTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H CAGAAATACTTTCTCAAAGTTCAGAAGAGTGGTGCTAACGAACATCTTCCACCTCCTCGACCTAAGAGGAAAGCGAGTCATCCTTATCCTATAAAGGCTC 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT4G01 CTAAAAATGTTGCTTATACCTCTCTCCCGTCTTCGAGTACATTACCGTTGCTTGAGCCTGGTTATTTGTATAGCTCTGATTCGAAGTCATTGATGGGAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H CTAAAAATGTTGCTTATACCTCTCTCCCGTCTTCGAGTACATTACCGTTGCTTGAGCCTGGTTATTTGTATAGCTCTGATTCGAAGTCATTGATGGGAAA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 AT4G01 CCAGGCTGTTTGTGCATCTACCTCTTCTTCGTGGAATCATGAATCGACAAATCTGCCAAAACCGGTGATTGA---AGAGGAACCGGGAGTCTCGGCCACG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::: IE_03H CCAGGCTGTTTGTGCATCTACCTCTTCTTCGTGGAATCATGAATCGACAAATCTGCCAAAACCGGTGATTGAAGTAGAGGAACCGGGAGTCTCGGCCACG 510 520 530 540 550 560 570 580 590 600 600 610 620 630 640 650 660 670 680 690 AT4G01 GCTCCTCTCCCAAATAATCGCTGCAGACAGGAAGATACAGAGAGGGTACGAGCAGTGACAAAGCCAAATAACGAAGAAAGTTGTGAAAAGCCACATAGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H GCTCCTCTCCCAAATAATCGCTGCAGACAGGAAGATACAGAGAGGGTACGAGCAGTGACAAAGCCAAATAACGAAGAAAGTTGTGAAAAGCCACATAGAG 610 620 630 640 650 660 670 680 690 700 700 710 720 730 740 750 760 770 780 790 AT4G01 TGATGCCGAATTTTGCTGAAGTTTACAGCTTCATTGGAAGTGTCTTCGATCCCAACACATCAGGCCACCTCCAGAGATTAAAGCAGATGGATCCAATAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H TGATGCCGAATTTTGCTGAAGTTTACAGCTTCATTGGAAGTGTCTTCGATCCCAACACATCAGGCCACCTCCAGAGATTAAAGCAGATGGATCCAATAAA 710 720 730 740 750 760 770 780 790 800 800 810 820 830 840 850 860 870 880 890 AT4G01 TATGGAAACGGTTCTTTTACTGATGCAAAACCTGTCTGTAAATCTGACAAGTCCCGAGTTTGCAGAGCAAAGGAGGTTGATATCATCATACAGCGCTAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H TATGGAAACGGTTCTTTTACTGATGCAAAACCTGTCTGTAAATCTGACAAGTCCCGAGTTTGCAGAGCAAAGGAGGTTGATATCATCATACAGCGCTAAA 810 820 830 840 850 860 870 880 890 900 900 AT4G01 GCTTTGAAA ::::::::: IE_03H GCTTTGAAA 10 20 30 40 50 60 70 80 90 100 AT4G01 MVSVNPRPKGFPVFDSSNMSLPSSDGFGSIPATGRTSTVSFSEDPTTKIRKPYTIKKSRENWTDQEHDKFLEALHLFDRDWKKIEAFVGSKTVVQIRSHA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H MVSVNPRPKGFPVFDSSNMSLPSSDGFGSIPATGRTSTVSFSEDPTTKIRKPYTIKKSRENWTDQEHDKFLEALHLFDRDWKKIEAFVGSKTVVQIRSHA 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 AT4G01 QKYFLKVQKSGANEHLPPPRPKRKASHPYPIKAPKNVAYTSLPSSSTLPLLEPGYLYSSDSKSLMGNQAVCASTSSSWNHESTNLPKPVIE-EEPGVSAT ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::: IE_03H QKYFLKVQKSGANEHLPPPRPKRKASHPYPIKAPKNVAYTSLPSSSTLPLLEPGYLYSSDSKSLMGNQAVCASTSSSWNHESTNLPKPVIEVEEPGVSAT 110 120 130 140 150 160 170 180 190 200 200 210 220 230 240 250 260 270 280 290 AT4G01 APLPNNRCRQEDTERVRAVTKPNNEESCEKPHRVMPNFAEVYSFIGSVFDPNTSGHLQRLKQMDPINMETVLLLMQNLSVNLTSPEFAEQRRLISSYSAK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H APLPNNRCRQEDTERVRAVTKPNNEESCEKPHRVMPNFAEVYSFIGSVFDPNTSGHLQRLKQMDPINMETVLLLMQNLSVNLTSPEFAEQRRLISSYSAK 210 220 230 240 250 260 270 280 290 300 300 AT4G01 ALK ::: IE_03H ALK

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