◆◆◆IE_03H07 detail◆◆◆

Code name	IE_03H07
Locus	AT4G00940
Template	IT_03H07 (HR3768)
Donor Vector	pDONR207
Forward primer	IPF_03H07 (ggggacaagtttgtacaaaaaagcaggcttcATGACTAGTACTAACAATAATTCCTATA)
Reverse primer	IPR_03H07 (ggggaccactttgtacaagaaagctgggttCCATGTTGGTCCACCACTATCACCACCTAG)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列94.60%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_03H07_834bp ATGACTAGTACTAACAATAATTCCTATAACACCATCGTCACAACACAACCACCACCAACAACAACAACAATGGATTCAACAACAGCAACAACTATGATAA TGGATGACGAGAAGAAGTTGATGACGACAATGAGCACTAGGCCGCAAGAACCAAGAAACTGTCCAAGATGCAACTCAAGCAACACCAAGTTTTGTTATTA CAACAACTACAGCTTAGCACAGCCTAGGTACTTGTGTAAGTCTTGTCGGAGATATTGGACTGAAGGTGGCTCTCTCCGTAACGTCCCCGTAGGCGGAGGT TCTAGAAAGAACAAGAAGCTTCCATTTCCTAATTCCTCTACTTCTTCTTCCACCAAGAACCTCCCGGATCTCAACCCTCCTTTCGTCTTCACATCATCAG CTTCATCATCAAACCCTAGCAAGACGCATCAAAACAATAATGACCTCAGCCTATCCTTCTCCTCCCCTATGCAAGACAAGCGAGCTCAAGGGCATTACGG TCATTTCAGTGAGCAAGTTGTGACAGGAGGGCAGAACTGTCTTTTCCAAGCTCCTATGGGAATGATTCAGTTTCGTCAAGAGTATGATCATGAGCACCCC AAAAAGAATCTTGGGTTTTCATTAGACAGGAACGAGGAAGAGATTGGTAATCATGATAACTTCGTTGTTAATGAGGAAGGAAGTAAGATGATGTATCCTT ATGGAGATCATGAAGACCGTCAACAACATCACCATGTGAGACACGATGATGGTAATAAGAAGAGAGAAGGTGGTTCAAGCAATGAGCTATGGAGCGGAAT CATCCTAGGTGGTGATAGTGGTGGACCAACATGG >IE_03H07_translated MTSTNNNSYNTIVTTQPPPTTTTMDSTTATTMIMDDEKKLMTTMSTRPQEPRNCPRCNSSNTKFCYYNNYSLAQPRYLCKSCRRYWTEGGSLRNVPVGGG SRKNKKLPFPNSSTSSSTKNLPDLNPPFVFTSSASSSNPSKTHQNNNDLSLSFSSPMQDKRAQGHYGHFSEQVVTGGQNCLFQAPMGMIQFRQEYDHEHP KKNLGFSLDRNEEEIGNHDNFVVNEEGSKMMYPYGDHEDRQQHHHVRHDDGNKKREGGSSNELWSGIILGGDSGGPTW
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 834/882 (94.6%)（アミノ酸配列で278/294 (94.6%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G00 ATGGACCATCATCAGTATCATCATCATGATCAATACCAACATCAGATGATGACTAGTACTAACAATAATTCCTATAACACCATCGTCACAACACAACCAC :::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H ------------------------------------------------ATGACTAGTACTAACAATAATTCCTATAACACCATCGTCACAACACAACCAC 10 20 30 40 50 110 120 130 140 150 160 170 180 190 200 AT4G00 CACCAACAACAACAACAATGGATTCAACAACAGCAACAACTATGATAATGGATGACGAGAAGAAGTTGATGACGACAATGAGCACTAGGCCGCAAGAACC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H CACCAACAACAACAACAATGGATTCAACAACAGCAACAACTATGATAATGGATGACGAGAAGAAGTTGATGACGACAATGAGCACTAGGCCGCAAGAACC 60 70 80 90 100 110 120 130 140 150 210 220 230 240 250 260 270 280 290 300 AT4G00 AAGAAACTGTCCAAGATGCAACTCAAGCAACACCAAGTTTTGTTATTACAACAACTACAGCTTAGCACAGCCTAGGTACTTGTGTAAGTCTTGTCGGAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H AAGAAACTGTCCAAGATGCAACTCAAGCAACACCAAGTTTTGTTATTACAACAACTACAGCTTAGCACAGCCTAGGTACTTGTGTAAGTCTTGTCGGAGA 160 170 180 190 200 210 220 230 240 250 310 320 330 340 350 360 370 380 390 400 AT4G00 TATTGGACTGAAGGTGGCTCTCTCCGTAACGTCCCCGTAGGCGGAGGTTCTAGAAAGAACAAGAAGCTTCCATTTCCTAATTCCTCTACTTCTTCTTCCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H TATTGGACTGAAGGTGGCTCTCTCCGTAACGTCCCCGTAGGCGGAGGTTCTAGAAAGAACAAGAAGCTTCCATTTCCTAATTCCTCTACTTCTTCTTCCA 260 270 280 290 300 310 320 330 340 350 410 420 430 440 450 460 470 480 490 500 AT4G00 CCAAGAACCTCCCGGATCTCAACCCTCCTTTCGTCTTCACATCATCAGCTTCATCATCAAACCCTAGCAAGACGCATCAAAACAATAATGACCTCAGCCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H CCAAGAACCTCCCGGATCTCAACCCTCCTTTCGTCTTCACATCATCAGCTTCATCATCAAACCCTAGCAAGACGCATCAAAACAATAATGACCTCAGCCT 360 370 380 390 400 410 420 430 440 450 510 520 530 540 550 560 570 580 590 600 AT4G00 ATCCTTCTCCTCCCCTATGCAAGACAAGCGAGCTCAAGGGCATTACGGTCATTTCAGTGAGCAAGTTGTGACAGGAGGGCAGAACTGTCTTTTCCAAGCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H ATCCTTCTCCTCCCCTATGCAAGACAAGCGAGCTCAAGGGCATTACGGTCATTTCAGTGAGCAAGTTGTGACAGGAGGGCAGAACTGTCTTTTCCAAGCT 460 470 480 490 500 510 520 530 540 550 610 620 630 640 650 660 670 680 690 700 AT4G00 CCTATGGGAATGATTCAGTTTCGTCAAGAGTATGATCATGAGCACCCCAAAAAGAATCTTGGGTTTTCATTAGACAGGAACGAGGAAGAGATTGGTAATC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H CCTATGGGAATGATTCAGTTTCGTCAAGAGTATGATCATGAGCACCCCAAAAAGAATCTTGGGTTTTCATTAGACAGGAACGAGGAAGAGATTGGTAATC 560 570 580 590 600 610 620 630 640 650 710 720 730 740 750 760 770 780 790 800 AT4G00 ATGATAACTTCGTTGTTAATGAGGAAGGAAGTAAGATGATGTATCCTTATGGAGATCATGAAGACCGTCAACAACATCACCATGTGAGACACGATGATGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H ATGATAACTTCGTTGTTAATGAGGAAGGAAGTAAGATGATGTATCCTTATGGAGATCATGAAGACCGTCAACAACATCACCATGTGAGACACGATGATGG 660 670 680 690 700 710 720 730 740 750 810 820 830 840 850 860 870 880 AT4G00 TAATAAGAAGAGAGAAGGTGGTTCAAGCAATGAGCTATGGAGCGGAATCATCCTAGGTGGTGATAGTGGTGGACCAACATGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H TAATAAGAAGAGAGAAGGTGGTTCAAGCAATGAGCTATGGAGCGGAATCATCCTAGGTGGTGATAGTGGTGGACCAACATGG 760 770 780 790 800 810 820 830 10 20 30 40 50 60 70 80 90 100 AT4G00 MDHHQYHHHDQYQHQMMTSTNNNSYNTIVTTQPPPTTTTMDSTTATTMIMDDEKKLMTTMSTRPQEPRNCPRCNSSNTKFCYYNNYSLAQPRYLCKSCRR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H ----------------MTSTNNNSYNTIVTTQPPPTTTTMDSTTATTMIMDDEKKLMTTMSTRPQEPRNCPRCNSSNTKFCYYNNYSLAQPRYLCKSCRR 10 20 30 40 50 60 70 80 110 120 130 140 150 160 170 180 190 200 AT4G00 YWTEGGSLRNVPVGGGSRKNKKLPFPNSSTSSSTKNLPDLNPPFVFTSSASSSNPSKTHQNNNDLSLSFSSPMQDKRAQGHYGHFSEQVVTGGQNCLFQA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H YWTEGGSLRNVPVGGGSRKNKKLPFPNSSTSSSTKNLPDLNPPFVFTSSASSSNPSKTHQNNNDLSLSFSSPMQDKRAQGHYGHFSEQVVTGGQNCLFQA 90 100 110 120 130 140 150 160 170 180 210 220 230 240 250 260 270 280 290 AT4G00 PMGMIQFRQEYDHEHPKKNLGFSLDRNEEEIGNHDNFVVNEEGSKMMYPYGDHEDRQQHHHVRHDDGNKKREGGSSNELWSGIILGGDSGGPTW :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_03H PMGMIQFRQEYDHEHPKKNLGFSLDRNEEEIGNHDNFVVNEEGSKMMYPYGDHEDRQQHHHVRHDDGNKKREGGSSNELWSGIILGGDSGGPTW 190 200 210 220 230 240 250 260 270

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