◆◆◆IE_04A02 detail◆◆◆

Code nameIE_04A02
LocusAT2G46270
TemplateIT_04A02 (HR0161)
Donor VectorpDONR207
Forward primerIPF_04A02 (ggggacaagtttgtacaaaaaagcaggcttcATGGGAAATAGCAGCGAGGAACCAAAGC)
Reverse primerIPR_04A02 (ggggaccactttgtacaagaaagctgggttGCCTGCAGCTACTGCTTTAGCTCGAGGCTT)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04A02_1146bp
ATGGGAAATAGCAGCGAGGAACCAAAGCCTCCTACCAAATCAGATAAACCATCTTCACCCCCGGTGGATCAAACAAATGTTCATGTCTACCCTGATTGGG
CAGCTATGCAGGCATATTATGGTCCAAGAGTAGCAATGCCTCCTTATTACAATTCAGCTATGGCTGCATCTGGTCATCCTCCTCCTCCTTACATGTGGAA
TCCTCAGCATATGATGTCACCATATGGAGCACCCTATGCTGCTGTTTATCCTCATGGAGGAGGAGTTTACGCTCATCCCGGTATTCCCATGGGATCACTG
CCTCAAGGTCAAAAGGATCCACCTTTAACAACTCCGGGGACGCTTTTGAGCATCGACACTCCTACTAAATCTACAGGGAACACAGACAATGGATTGATGA
AGAAGCTGAAAGAGTTTGATGGGCTTGCTATGTCTCTAGGAAATGGGAATCCTGAAAATGGTGCAGATGAACATAAACGATCACGGAACAGCTCAGAAAC
TGATGGTTCTACTGATGGAAGTGATGGGAATACAACTGGGGCAGATGAACCGAAACTTAAAAGAAGTCGAGAGGGAACTCCAACAAAAGATGGGAAACAA
TTGGTTCAAGCTAGCTCATTTCATTCTGTTTCTCCGTCAAGTGGTGATACCGGCGTAAAACTCATTCAAGGATCTGGAGCTATACTCTCTCCTGGTGTAA
GTGCAAATTCCAACCCCTTCATGTCACAATCTTTAGCCATGGTTCCTCCTGAAACTTGGCTTCAGAACGAGAGAGAACTGAAACGGGAGCGAAGGAAACA
GTCTAATAGAGAATCTGCTAGAAGGTCAAGATTAAGGAAACAGGCCGAGACAGAAGAACTTGCTAGGAAAGTGGAAGCCTTGACAGCCGAAAACATGGCA
TTAAGATCTGAACTAAACCAACTTAATGAGAAATCTGATAAACTAAGAGGAGCAAATGCAACCTTGTTGGACAAACTGAAATGCTCGGAACCCGAAAAGA
GAGTCCCCGCAAATATGTTGTCTAGAGTTAAGAACTCAGGAGCTGGAGATAAGAACAAGAACCAAGGAGACAATGATTCTAACTCTACAAGCAAATTGCA
TCAACTGCTCGATACGAAGCCTCGAGCTAAAGCAGTAGCTGCAGGC

>IE_04A02_translated
MGNSSEEPKPPTKSDKPSSPPVDQTNVHVYPDWAAMQAYYGPRVAMPPYYNSAMAASGHPPPPYMWNPQHMMSPYGAPYAAVYPHGGGVYAHPGIPMGSL
PQGQKDPPLTTPGTLLSIDTPTKSTGNTDNGLMKKLKEFDGLAMSLGNGNPENGADEHKRSRNSSETDGSTDGSDGNTTGADEPKLKRSREGTPTKDGKQ
LVQASSFHSVSPSSGDTGVKLIQGSGAILSPGVSANSNPFMSQSLAMVPPETWLQNERELKRERRKQSNRESARRSRLRKQAETEELARKVEALTAENMA
LRSELNQLNEKSDKLRGANATLLDKLKCSEPEKRVPANMLSRVKNSGAGDKNKNQGDNDSNSTSKLHQLLDTKPRAKAVAAG
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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