◆◆◆IE_04A05 detail◆◆◆

Code nameIE_04A05
LocusAT1G77920
TemplateIT_04A05 (HR0609)
Donor VectorpDONR207
Forward primerIPF_04A05 (ggggacaagtttgtacaaaaaagcaggcttcATGATGAGTTCTTCTTCTCCAACACAAC)
Reverse primerIPR_04A05 (ggggaccactttgtacaagaaagctgggttAGTTGGTTCTTGTGGACGAGCTGCCCAAAG)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04A05_1104bp
ATGATGAGTTCTTCTTCTCCAACACAACTTGCATCTTTAAGAGACATGGGAATCTATGAGCCATTTCAACAAATTGTCGGTTGGGGAAATGTTTTCAAAT
CTGATATCAATGATCATAGTCCCAATACTGCTACTTCCTCTATTATTCAGGTTGATCCTAGAATTGATGATCACAACAACAACATCAAGATAAATTATGA
TTCTTCTCATAACCAGATCGAAGCAGAACAACCTTCTAGTAATGATAATCAAGATGATGATGGCAGGATTCATGATAAGATGAAACGGCGTTTAGCGCAG
AACCGAGAAGCGGCTCGCAAAAGTCGTTTGAGAAAGAAGGCTTATGTTCAGCAGTTAGAGGAAAGCCGGTTAAAGTTATCGCAGTTAGAGCAAGAACTCG
AAAAGGTTAAGCAGCAGGGCCATTTAGGACCATCTGGGAGTATTAACACAGGGATTGCATCATTTGAGATGGAATATTCACACTGGCTACAAGAACAAAG
CAGAAGAGTTAGCGAACTACGAACAGCGCTTCAATCTCATATAAGCGACATAGAACTCAAGATGCTAGTAGAGAGTTGCTTGAACCATTACGCTAATCTT
TTCCAAATGAAATCCGATGCAGCAAAAGCCGATGTTTTCTACTTGATATCGGGAATGTGGCGAACTTCAACCGAAAGATTCTTCCAATGGATTGGAGGGT
TTCGTCCATCCGAACTTTTAAACGTTGTGATGCCTTATCTTCAACCATTAACGGATCAACAAATCTTGGAAGTGAGAAACCTCCAACAATCATCACAACA
AGCAGAGGATGCTCTGTCTCAAGGGATTGATAAACTTCAACAGAGTTTAGCTGAAAGCATTGTGATTGATGCGGTTATCGAGTCCACGCATTATCCCACT
CACATGGCTGCAGCTATAGAGAATCTTCAAGCATTAGAAGGATTTGTGAATCAAGCAGATCATCTGAGGCAACAAACTTTGCAACAAATGGCGAAGATCT
TAACGACAAGACAATCGGCTCGAGGTTTACTAGCTTTAGGAGAGTATCTTCATAGACTTCGTGCTCTTAGTTCTCTTTGGGCAGCTCGTCCACAAGAACC
AACT

>IE_04A05_translated
MMSSSSPTQLASLRDMGIYEPFQQIVGWGNVFKSDINDHSPNTATSSIIQVDPRIDDHNNNIKINYDSSHNQIEAEQPSSNDNQDDDGRIHDKMKRRLAQ
NREAARKSRLRKKAYVQQLEESRLKLSQLEQELEKVKQQGHLGPSGSINTGIASFEMEYSHWLQEQSRRVSELRTALQSHISDIELKMLVESCLNHYANL
FQMKSDAAKADVFYLISGMWRTSTERFFQWIGGFRPSELLNVVMPYLQPLTDQQILEVRNLQQSSQQAEDALSQGIDKLQQSLAESIVIDAVIESTHYPT
HMAAAIENLQALEGFVNQADHLRQQTLQQMAKILTTRQSARGLLALGEYLHRLRALSSLWAARPQEPT
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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