◆◆◆IE_04A09 detail◆◆◆

Code name	IE_04A09
Locus	AT5G06420
Template	IT_04A09 (HR0948)
Donor Vector	pDONR207
Forward primer	IPF_04A09 (ggggacaagtttgtacaaaaaagcaggcttcATGGAACCATTCGTTGTTAAGGAGAACA)
Reverse primer	IPR_04A09 (ggggaccactttgtacaagaaagctgggttCAATCCTTGTTCAGCTTTACTCCGTTCTTC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.50%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_04A09_1134bp ATGGAACCATTCGTTGTTAAGGAGAACATCGTCGCATCCGCTTCTTCGCCGATGAAGAAGCGACGGATCGATCACACTGAGTCAGCTGATGGTTCTGCGA TTAACGCTTCTAACTCTAGTAGCATCGGTGGTAACGATACGGTAATGAACATGGCGGAGTTTGGTAACGACAACTCCAACAATCAGGAGTCTCAACAAGT TTGCACTTTCTTCAAGAAGCCAACGAAAAGTAAAAACATAAGGAAAAGAACCATTGACGCTGATGAAGAAGACGGAGATTCGAAAAGCGAAAGCTCTATT TTACAAAATCTAAAGAAAGTTGCAAAACCCGATAGCAACTTGTACTTTTCTTCTGGACCATCTACTAGAACGAGTGGAGCTCCTGAGAGACCCGTTTTTC ACTACGATTCATCCAAGGAAATCCAGGTTCAGAACGACAGTGGAGCAACAGCGACCCTTGAAACTGAAACCGACTTCAATCAAGACGCTCGAGCTATCCG TGAAAGAGTTCTTAAAAAAGCAGACCATGCATTGAAGGGGAATAAGAAAAAGGCTTCAGATGAGAAGCTGTACAAAGGAATTCATGGATATACAGATCAC AAAGCTGGGTTTAGAAGAGAACAAACAATCTCGAGCGAGAAAGCTGAAGGCTCACACGGGCCTTTAAGAGCTTCTGCTCACATCAGAGTATCGGCTAGAT TCGATTACCAGCCAGACATTTGCAAGGATTACAAGGAAACCGGATACTGTGGATATGGAGATTCGTGTAAGTTCTTGCATGACCGTGGGGATTACAAGCC GGGATGGCAGATAGAGAAAGAGTGGGAAGAGGCAGAGAAAGTTAGGAAGAGAAATAAAGCTATGGGAGTTGAGGATGACGATGATGAGGCTGACAAGGAT AGCGACGAAGACGAAAATGCATTGCCCTTTGCTTGCTTCATTTGCAGGGACCCTTTTCTTGATCCAGTTGTCACCAAATGCAAGCATTACTTCTGTGAGC ATTGTGCTTTAAAGCATCACACGAAGAACAAGAAATGCTTTGTGTGTAACCAACCAACAATGGGGATTTTCAATGCAGCACATGAGATCAAGAAGAGAAT GGCTGAAGAACGGAGTAAAGCTGAACAAGGATTG >IE_04A09_translated MEPFVVKENIVASASSPMKKRRIDHTESADGSAINASNSSSIGGNDTVMNMAEFGNDNSNNQESQQVCTFFKKPTKSKNIRKRTIDADEEDGDSKSESSI LQNLKKVAKPDSNLYFSSGPSTRTSGAPERPVFHYDSSKEIQVQNDSGATATLETETDFNQDARAIRERVLKKADHALKGNKKKASDEKLYKGIHGYTDH KAGFRREQTISSEKAEGSHGPLRASAHIRVSARFDYQPDICKDYKETGYCGYGDSCKFLHDRGDYKPGWQIEKEWEEAEKVRKRNKAMGVEDDDDEADKD SDEDENALPFACFICRDPFLDPVVTKCKHYFCEHCALKHHTKNKKCFVCNQPTMGIFNAAHEIKKRMAEERSKAEQGL
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1131/1134 (99.7%)（アミノ酸配列で376/378 (99.5%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G06 ATGGAACCATTCGTTGTTAAGGAGAACATCGTCGCATCCGCTTCTTCGCCGATGAAGAAGCGACGGATCGATCACACTGAGTCAGCTGATGGTTCTGCGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A ATGGAACCATTCGTTGTTAAGGAGAACATCGTCGCATCCGCTTCTTCGCCGATGAAGAAGCGACGGATCGATCACACTGAGTCAGCTGATGGTTCTGCGA 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G06 TTAACGCTTCTAACTCTAGTAGCATCGGTGGTAACGATACGGTGATGAACATGGCGGAGTTTGGTAACGACAACTCCAACAATCAGGAGTCTCAACAAGT ::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A TTAACGCTTCTAACTCTAGTAGCATCGGTGGTAACGATACGGTAATGAACATGGCGGAGTTTGGTAACGACAACTCCAACAATCAGGAGTCTCAACAAGT 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G06 TTGCACTTTCTTCAAGAAGCCAACGAAAAGTAAAAACATAAGGAAAAGAACCATTGACGCTGATGAAGAAGACGGAGATTCGAAAAGCGAAAGCTCTATT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A TTGCACTTTCTTCAAGAAGCCAACGAAAAGTAAAAACATAAGGAAAAGAACCATTGACGCTGATGAAGAAGACGGAGATTCGAAAAGCGAAAGCTCTATT 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G06 TTACAAAATCTAAAGAAAGTTGCAAAACCCGATAGCAACTTGTACTTTTCTTCTGGACCATCTACTAGAACGAGTGGAGCTCCTGAGAGACCCGTTTTTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A TTACAAAATCTAAAGAAAGTTGCAAAACCCGATAGCAACTTGTACTTTTCTTCTGGACCATCTACTAGAACGAGTGGAGCTCCTGAGAGACCCGTTTTTC 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G06 ACTACGATTCATCCAAGGAAATCCAGGTTCAGAACGACAGTGGAGCAACAGCGACCCTTGAAACTGAAACCGACTTCAATCAAGACGCTCGAGCTATCCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A ACTACGATTCATCCAAGGAAATCCAGGTTCAGAACGACAGTGGAGCAACAGCGACCCTTGAAACTGAAACCGACTTCAATCAAGACGCTCGAGCTATCCG 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G06 TGAAAGAGTTCTTAAAAAAGCAGACCATGCATTGAAGGGGAATAAGAAAAAGGCTTCAGATGAGAAGCTGTACAAAGGAATTCATGGATATACAGATCAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A TGAAAGAGTTCTTAAAAAAGCAGACCATGCATTGAAGGGGAATAAGAAAAAGGCTTCAGATGAGAAGCTGTACAAAGGAATTCATGGATATACAGATCAC 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G06 AAAGCTGGGTTTAGAAGAGAACAAACAATCTCGAGCGAGAAAGCTGGAGGCTCACACGGGCCTTTAAGAGCTTCTGCTCACATCAGAGTATCGGCTAGAT :::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A AAAGCTGGGTTTAGAAGAGAACAAACAATCTCGAGCGAGAAAGCTGAAGGCTCACACGGGCCTTTAAGAGCTTCTGCTCACATCAGAGTATCGGCTAGAT 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT5G06 TCGATTACCAGCCAGACATTTGCAAGGATTACAAGGAAACCGGATACTGTGGATATGGAGATTCGTGTAAGTTCTTGCATGACCGTGGGGATTACAAGCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A TCGATTACCAGCCAGACATTTGCAAGGATTACAAGGAAACCGGATACTGTGGATATGGAGATTCGTGTAAGTTCTTGCATGACCGTGGGGATTACAAGCC 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT5G06 GGGATGGCAGATAGAGAAAGAGTGGGAAGAGGCAGAGAAAGTTAGGAAGAGAAATAAAGCTATGGGAGTTGAGGATGACGATGATGAGGCTGACAAGGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A GGGATGGCAGATAGAGAAAGAGTGGGAAGAGGCAGAGAAAGTTAGGAAGAGAAATAAAGCTATGGGAGTTGAGGATGACGATGATGAGGCTGACAAGGAT 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT5G06 AGCGACGAAGACGAAAATGCATTGCCCTTTGCTTGCTTCATTTGCAGGGAGCCTTTTCTTGATCCAGTTGTCACCAAATGCAAGCATTACTTCTGTGAGC :::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A AGCGACGAAGACGAAAATGCATTGCCCTTTGCTTGCTTCATTTGCAGGGACCCTTTTCTTGATCCAGTTGTCACCAAATGCAAGCATTACTTCTGTGAGC 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT5G06 ATTGTGCTTTAAAGCATCACACGAAGAACAAGAAATGCTTTGTGTGTAACCAACCAACAATGGGGATTTTCAATGCAGCACATGAGATCAAGAAGAGAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A ATTGTGCTTTAAAGCATCACACGAAGAACAAGAAATGCTTTGTGTGTAACCAACCAACAATGGGGATTTTCAATGCAGCACATGAGATCAAGAAGAGAAT 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 AT5G06 GGCTGAAGAACGGAGTAAAGCTGAACAAGGATTG :::::::::::::::::::::::::::::::::: IE_04A GGCTGAAGAACGGAGTAAAGCTGAACAAGGATTG 1110 1120 1130 10 20 30 40 50 60 70 80 90 100 AT5G06 MEPFVVKENIVASASSPMKKRRIDHTESADGSAINASNSSSIGGNDTVMNMAEFGNDNSNNQESQQVCTFFKKPTKSKNIRKRTIDADEEDGDSKSESSI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A MEPFVVKENIVASASSPMKKRRIDHTESADGSAINASNSSSIGGNDTVMNMAEFGNDNSNNQESQQVCTFFKKPTKSKNIRKRTIDADEEDGDSKSESSI 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G06 LQNLKKVAKPDSNLYFSSGPSTRTSGAPERPVFHYDSSKEIQVQNDSGATATLETETDFNQDARAIRERVLKKADHALKGNKKKASDEKLYKGIHGYTDH :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A LQNLKKVAKPDSNLYFSSGPSTRTSGAPERPVFHYDSSKEIQVQNDSGATATLETETDFNQDARAIRERVLKKADHALKGNKKKASDEKLYKGIHGYTDH 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G06 KAGFRREQTISSEKAGGSHGPLRASAHIRVSARFDYQPDICKDYKETGYCGYGDSCKFLHDRGDYKPGWQIEKEWEEAEKVRKRNKAMGVEDDDDEADKD ::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A KAGFRREQTISSEKAEGSHGPLRASAHIRVSARFDYQPDICKDYKETGYCGYGDSCKFLHDRGDYKPGWQIEKEWEEAEKVRKRNKAMGVEDDDDEADKD 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 AT5G06 SDEDENALPFACFICREPFLDPVVTKCKHYFCEHCALKHHTKNKKCFVCNQPTMGIFNAAHEIKKRMAEERSKAEQGL ::::::::::::::::.::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04A SDEDENALPFACFICRDPFLDPVVTKCKHYFCEHCALKHHTKNKKCFVCNQPTMGIFNAAHEIKKRMAEERSKAEQGL 310 320 330 340 350 360 370

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