◆◆◆IE_04A12 detail◆◆◆

Code nameIE_04A12
LocusAT2G20400
TemplateIT_04A12 (HR1115)
Donor VectorpDONR207
Forward primerIPF_04A12 (ggggacaagtttgtacaaaaaagcaggcttcATGATCCCGAATGATGATGATGATGCAA)
Reverse primerIPR_04A12 (ggggaccactttgtacaagaaagctgggttTTCTTCATTTCTCGGACGCTTTGGCCGCGG)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04A12_1191bp
ATGATCCCGAATGATGATGATGATGCAAATTCTATGAAGAATTATCCGTTAAATGATGATGATGCAAATTCTATGAAGAATTATCCGTTAAATGATGATG
ATGCAAATTCTATGGAGAATTATCCGTTAAGGTCAATTCCGACGGAGCTTTCACACACTTGTTCATTGATACCACCTTCTTTACCAAACCCTTCAGAAGC
AGCAGCAGACATGTCCTTCAATTCAGAACTCAATCAAATCATGGCAAGGCCTTGTGATATGCTCCCTGCCAATGGTGGAGCTGTTGGTCATAACCCTTTT
TTGGAACCAGGATTCAACTGCCCCGAGACAACAGATTGGATTCCCTCTCCACTCCCCCATATTTATTTTCCTTCGGGTTCTCCCAATCTAATAATGGAGG
ATGGTGTCATTGATGAGATTCACAAACAAAGTGACTTGCCACTTTGGTATGACGACTTGATTACCACTGATGAAGATCCACTCATGTCTAGTATCTTGGG
CGATCTTCTCCTTGACACTAATTTCAACTCAGCTTCAAAGGTCCAGCAACCAAGTATGCAATCGCAGATTCAACAACCCCAAGCTGTTCTGCAGCAGCCT
TCTTCTTGTGTGGAATTGCGCCCACTTGATAGGACAGTATCCTCAAACAGCAACAACAATAGCAACAGTAATAATGCAGCAGCAGCAGCTAAGGGACGTA
TGCGTTGGACGCCTGAACTTCATGAGGTTTTTGTTGACGCTGTTAACCAGCTCGGTGGCAGTAATGAAGCAACTCCTAAAGGTGTCCTGAAGCATATGAA
AGTCGAAGGTTTGACTATTTTTCATGTCAAAAGTCATTTGCAGAAATATAGAACAGCTAAATATATACCAGTACCATCAGAAGGTTCGCCGGAGGCAAGG
TTGACACCGCTTGAGCAAATTACATCTGATGATACGAAACGTGGGATAGATATCACTGAGACTCTGCGAATTCAGATGGAACATCAGAAGAAACTGCATG
AGCAGCTTGAGAGTCTAAGAACAATGCAACTTCGGATAGAAGAGCAAGGAAAGGCGCTGTTGATGATGATTGAGAAGCAAAATATGGGTTTCGGCGGACC
AGAACAAGGAGAGAAAACAAGTGCGAAAACGCCTGAAAATGGTTCAGAGGAGTCGGAATCCCCGCGGCCAAAGCGTCCGAGAAATGAAGAA

>IE_04A12_translated
MIPNDDDDANSMKNYPLNDDDANSMKNYPLNDDDANSMENYPLRSIPTELSHTCSLIPPSLPNPSEAAADMSFNSELNQIMARPCDMLPANGGAVGHNPF
LEPGFNCPETTDWIPSPLPHIYFPSGSPNLIMEDGVIDEIHKQSDLPLWYDDLITTDEDPLMSSILGDLLLDTNFNSASKVQQPSMQSQIQQPQAVLQQP
SSCVELRPLDRTVSSNSNNNSNSNNAAAAAKGRMRWTPELHEVFVDAVNQLGGSNEATPKGVLKHMKVEGLTIFHVKSHLQKYRTAKYIPVPSEGSPEAR
LTPLEQITSDDTKRGIDITETLRIQMEHQKKLHEQLESLRTMQLRIEEQGKALLMMIEKQNMGFGGPEQGEKTSAKTPENGSEESESPRPKRPRNEE
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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