◆◆◆IE_04B04 detail◆◆◆

Code nameIE_04B04
LocusAT5G04390
TemplateIT_04B04 (HR1234)
Donor VectorpDONR207
Forward primerIPF_04B04 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAGCGTTCGAAGAGGCCACAAAGG)
Reverse primerIPR_04B04 (ggggaccactttgtacaagaaagctgggttGTAGTGGCAATCCACCAAAGTAGGAGCAGA)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04B04_1086bp
ATGGAAGCGTTCGAAGAGGCCACAAAGGAGCAATCTTTGATCCTTAAAGGGAAGCGGACGAAGCGACAACGTCCACAATCTCCTATTCCTTTCTCTATCT
CCCCTCCTATAGTTTCTACCCCGGAAAACAACATGGAAGAAGAATACACTGATCTTGATTCCAAGGACAATGCTTTAGGAAATGATGAGGGCAACCACAA
AAAGGATGGTGTGATCACATCTTCATCTTCATCAGCCTCTTGGTCCTCCCAAAACAATCACACATTGAAGGCAGCAGAAGACGAGGAAGATCAAGACATA
GCCAATTGTTTGATACTCCTTGCCCAAGGACACTCTCTTCCCCACAACAATCACCACTTACCAAACAGCAACAACAACAACACGTATAGATTTACCAGCA
GGAGGTTTCTAGAAACTTCTTCTTCTAACAGTGGTGGAAAAGCCGGTTACTACGTTTATCAGTGCAAGACATGTGACCGGACCTTCCCTTCTTTTCAGGC
TTTAGGTGGCCACAGAGCTAGCCACAAGAAACCTAAAGCCGCCTCTTTTTACTCCAACCTTGACCTCAAGAAGAACACGTATGCAAACGACGCCGTTTCA
CTTGTACACACAACCACAACTGTTTTCAAGAACAATAACAGTAGATCACTTGTCGTGTACGGTAAGGCAAGTAAAAATAAGGTTCATGAATGTGGAATCT
GTGGAGCCGAGTTTACGTCCGGACAAGCCTTAGGTGGCCACATGAGACGGCATAGAGGAGCTGTGGTTGTTCCGGCGGTGATAGCCCCCACCGTGACGGT
GGCCACGGCTGCGGCCAACACGGAGCTATCACTGTCTTCGATGTCGTTCGATCAAATATCGGACGGTCATCAGGATCATCTGGCGATGCCAGCGAAAAAG
AAAGCTAGGACGGTCGTGTCACTGGATTTGGATCTGAATCTACCAGCACCGGAAGATGAGAATCGGGTCAACGGATTCAGCTTTGCTTCAAAGCAAAATC
ACGAACAAGAACATCAACAGAAGAAGCAAAGAGAAGAACCAAAGTCTCTTGTCTTGTCTGCTCCTACTTTGGTGGATTGCCACTAC

>IE_04B04_translated
MEAFEEATKEQSLILKGKRTKRQRPQSPIPFSISPPIVSTPENNMEEEYTDLDSKDNALGNDEGNHKKDGVITSSSSSASWSSQNNHTLKAAEDEEDQDI
ANCLILLAQGHSLPHNNHHLPNSNNNNTYRFTSRRFLETSSSNSGGKAGYYVYQCKTCDRTFPSFQALGGHRASHKKPKAASFYSNLDLKKNTYANDAVS
LVHTTTTVFKNNNSRSLVVYGKASKNKVHECGICGAEFTSGQALGGHMRRHRGAVVVPAVIAPTVTVATAAANTELSLSSMSFDQISDGHQDHLAMPAKK
KARTVVSLDLDLNLPAPEDENRVNGFSFASKQNHEQEHQQKKQREEPKSLVLSAPTLVDCHY
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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